Korean Journal Plant Pathology (한국식물병리학회지)

The Korean Society of Plant Pathology (한국식물병리학회)
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An Effective Detection of Potato Virus Y Using RT-PCR Technique

RT-PCR 기법을 이용한 효과적인 감자바이러스 Y의 검정

  • Joung, Young-Hee (Plant Tissue Culture Unit, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Jeon, Jae-Heung (Plant Tissue Culture Unit, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Choi, Kyung-Hwa (Plant Tissue Culture Unit, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Kim, Hyun-Soon (Plant Tissue Culture Unit, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Yi, Yong-Sub (Plant Tissue Culture Unit, Korea Research Institute of Bioscience & Biotechnology (KRIBB)) ;
  • Joung, Hyouk (Plant Tissue Culture Unit, Korea Research Institute of Bioscience & Biotechnology (KRIBB))
  • 정영희 (생명공학연구소 식물조직배양 연구 Unit) ;
  • 전재홍 (생명공학연구소 식물조직배양 연구 Unit) ;
  • 최경화 (생명공학연구소 식물조직배양 연구 Unit) ;
  • 김현순 (생명공학연구소 식물조직배양 연구 Unit) ;
  • 이용섭 (생명공학연구소 식물조직배양 연구 Unit) ;
  • 정혁 (생명공학연구소 식물조직배양 연구 Unit)
  • Published : 1997.08.01
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Abstract

A PT-PCR (reverse transcription-polymerase chain reaction) diagnostic method for potato virus Y (PVY) was developed using primer pair derived from conserved region of coat protein genes of several PVY strains, A 764 bp PCR product was detected from several lines of potato cv. Atlantic. We could prove that the 764 bp DNA fragment was indeed the PVY gene by sequencing analysis. PVY detection method using RT-PCR technique was about tuber tissue.

여러 계통의 PVY 외피단백질 유전자간에 상동성이 높은 부위에 위치한 primer 쌍을 이용하여 RT-PCR 방법으로 PVY 검정법을 개발하였는데 여러 line의 대서품종으로부터 764 bp의 PCR 산물이 합성되었고 이 절편을 sequencing한 결가 PVY의 유전자임을 확인하였다. 싹과 괴경 조직에서 RT-PCR에 의한 PVY의 검정의 민감도는 ELISA 방법보다 약 1,000배정도 높았다.

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