• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.161-165
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• 1969

Since Guha and Maheshwari's works on the induction of haploid plant from the cultured anther of Datura innoxia an her culture has become a big topic among geneticists and breeders. Presen paper is the summarized report on the anther culture method based on the author's recent researches.

• The Journal of the Korea Contents Association
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• v.11 no.6
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• pp.438-448
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• 2011

The purpose of this paper is to enrich storytelling contents for culture and tourism industry and provide the implication for carrying out governmental policy by estimating economic value of storytelling using a Contingent Valuation Method. The empirical result shows that the economic value of storytelling development in culture and tourism industry is annually estimated 16,000 won per person, resulting from logit model and WTP mean method. The willingness-to-pay, degree of support and perception of the policy and culture contents expenditure have meaningful effects on probability of supporting and participating in execution of the policy. The findings imply that the economic value of storytelling development highly increases as the interest and expenditure of culture contents increase. Futhermore, informing storytelling related policy and business to the people will increase economic value of fostering storytelling-based culture and tourism.

• The Korean Journal of Archival Studies
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• no.24
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• pp.173-214
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• 2010

Intangible Culture Heritage can show its face only by representation of people in no form. Accordingly, if there are not people who represent Intangible Culture Heritage or records which is about Intangible Culture Heritage, we can see its appearance any more. Now on, Intangible Culture Heritage preservation policy of Korea is incomplete, so polices that Intangible Culture Heritage keeps on its values permanently, are essential. This study starting from these critical mind, suggests documentation method of Gangneung Danoje. This study designs documentation plans of Intangible Culture Heritage considering whore its whole lifecyle that produced, keened, used, preserved. To document the Gangneung Danoje, the target of documentation is selected and divided as ceremony, transmit from generation to generation, related data. the ceremony and transmit from generation to generation of Gangneung Danoje are recognized by building the business process, so records that produces whenever business act produce, subjects who produce the records, the spot where records are produced. A related data is not the target of documentation produced as continual business, but something to worthy of keeping as records is selected. Through investigation of the ceremony, transmit from generation to generation, and related data, this study selects objects to documentation and suggests method of documentation and subjects who document records.

• Journal of the Korean Society of Food Culture
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• v.25 no.4
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• pp.400-409
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• 2010

This study investigated the food and dietary educational content in primary, middle and high school textbooks in order to provide fundamental data for the development of educational programs on Korean traditional food culture. The research objects consisted of 51 kinds of textbooks (15 kinds of primary school textbooks, 29 kinds of middle school home economics textbooks and 7 kinds of high school home economics textbooks), and the contents related to food and dietary education were counted and analyzed. The content analysis was performed using two categories: application method and subject matter. Application method included texts, cases, visual aids (pictures, photos, illustrations, chart, etc.) and activities, whereas subject matter consisted of seven types (well balanced nutrition and health, understanding of food and nutrition, cooking principles, cooking lessons, traditional foods and culture, others). The results of the application method in primary school textbooks show that visual aids were the most common in all six grades. For the subject matter, 'understanding of food and nutrition' was most abundant in primary school textbooks while 'well balanced nutrition and health' accounted for a large part of the contents in middle school textbooks. However, the contents regarding traditional foods and culture were insufficient in primary and middle school textbooks. These results suggest that educational contents on traditional foods and culture should be added to primary and middle school textbooks and covered in various subjects. Furthermore, high school 'home economics' contents need to emphasize comprehensive food and dietary education and adjust to 'science & technology for life'.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.560-567
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• 2008

An efficient method to produce water-soluble polysaccharides from Lentinus lepideus is described. The productivity of both endopolysaccharides (PPS) and exopolysaccharides (EPS) was compared under various culture conditions. The effect of treating their own PPS and EPS on immune cytokine production was also studied in relation to culture factors. High yield production of EPS required a moderate culture temperature $(25^{\circ}C)$ as well as long culture period (16-20 days). In contrast, PPS production required a high culture temperature $(30^{\circ}C)$ and short culture period (8 days). Most of the carbon sources did not affect polysaccharides and mycelial production except for sucrose. Immune cytokine levels in the EPS treatment varied among carbon sources or culture periods. PPS did not appear to affect much on the production of cytokines, regardless of the culturing factors, except for the culture period. These results suggest that the optimal culture conditions for L. lepideus vary according to culture purposes, and different culture conditions should be used for different targets including mycelial biomass, EPS, and PPS. Whereas the immunomodulating activitiy of EPS appeared to be affected by culture conditions in L. lepideus, that of PPS did not.

• Journal of Korean Academy of Nursing Administration
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• v.17 no.1
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• pp.88-95
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• 2011

Purpose: This study was done to identify the effect of nursing organizational culture on empowerment as perceived by new clinical nurses. Method: For the objective a sample of 175 new nurses from secondary/tertiary hospitals having not less than 250 beds located in B city was selected to complete a questionnaire. The collected data were analyzed using descriptive statistics, t-test, One-way ANOVA, Scheffe's test, Pearson correlation coefficients and simultaneous multiple regression with the SPSS 14.0 software program. Results: As for nursing organizational culture as perceived by the respondent, relation-oriented culture had the highest average score ($3.60{\pm}.63$) out of a possible 5 points followed by hierarch-oriented culture, innovation-oriented culture and task-oriented culture with $3.45{\pm}.30$, $3.19{\pm}.53$ and $3.05{\pm}.46$, respectively. The respondents' level of perceived empowerment was $3.06{\pm}.41$. The factors influencing empowerment according to nursing organizational culture were innovation-oriented and relation-oriented culture in that order. These 2 variables were statistically significant predictors and explained 48.6% of the variance in empowerment. Conclusions: The results of this study indicate that emphasis should be given to nursing organizational culture strengthened by innovationoriented and relation-oriented culture, rather than task-oriented and hierarch-oriented culture, in order to foster harmonious empowerment among nurses at all levels.

• Journal of Korean language education
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• v.29 no.1
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• pp.53-84
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• 2018

The main purpose of this study is to investigate foreigners' perception regarding Korean business culture and Korean business culture education. People who participated in this study are six foreign workers after studying at the university or graduate school in Korea. A phenomenological qualitative research method was used in this study in order to elicit life experiences from foreign workers who were working in Korea business companies. Therefore, their experience regarding Korean business culture and business culture education were measured by in-depth interviews. This study shows that nun-chi, vertical relationships, and impatience of Koreans are the most difficult culture to foreign workers. They also feel difficulties regarding Koreans' lack of understanding about other cultures. Moreover, these were not difficult components within school life; rather, the most difficult aspects were that they have never learned about the Korean business culture before getting a job. The results show that international students who want to work in Korea companies need to study about the culture of Korean business and revealed the desirability to learn. These results can be used as a foundation for understanding some contents and criterion of Korean business culture and education.

• Journal of Microbiology and Biotechnology
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• v.28 no.1
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• pp.32-39
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• 2018

Samples of Laru (a fermentation starter) obtained from the upper part of Borneo Island were analyzed for their lactic acid bacteria (LAB) and fungal diversity using both a culture-independent method (PCR-DGGE) and culture-dependent methods (SDS-PAGE and MALDI-TOF MS). Pediococcus pentosaceus, Lactobacillus brevis, Saccharomycopsis fibuligera, Hyphopichia burtonii, and Kodamaea ohmeri were detected by all three methods. In addition, Weissella cibaria, Weissella paramesenteroides, Leuconostoc citreum, Leuconostoc mesenteroides, Lactococcus lactis, Rhizopus oryzae/Amylomyces rouxii, Mucor indicus, and Candida intermedia were detected by PCR-DGGE. In contrast, Lactobacillus fermentum, Lactobacillus plantarum, Pichia anomala, Candida parapsilosis, and Candida orthopsilosis were detected only by the culture-dependent methods. Our results indicate that the culture-independent method can be used to determine whether multiple laru samples originated from the same manufacturing region; however, using the culture-independent and the two culture-dependent approaches in combination provides a more comprehensive overview of the laru microbiota.

• Animal cells and systems
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• v.13 no.3
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• pp.267-274
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• 2009

The dissociative culture technique of Aplysia neuron is one of the key methods that have been used for studies of cellular and molecular mechanisms of neuronal functioning. However, despite the advantages this method offers as an experimental model, its technical efficiency has had room for improvement. In this study, we examined certain putative factors that might affect the culture quality. The effects of neuronal damage induced by physical injuries, heat shock, and surface protein degradation were evaluated along with the correlation between the culture quality and animal behavior. As a result, we found that physical injury can be a critical factor that affects culture quality, whereas the heat shock and surface protein degradation had negligible effect on it. In addition, we discovered that siphon retraction time was not a good measurement for healthy neurons. Based on these findings, we suggest here an improved method in which the degree of physical injury is reduced by means of multiple protease treatment.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.219-224
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• 1997

A PT-PCR (reverse transcription-polymerase chain reaction) diagnostic method for potato virus Y (PVY) was developed using primer pair derived from conserved region of coat protein genes of several PVY strains, A 764 bp PCR product was detected from several lines of potato cv. Atlantic. We could prove that the 764 bp DNA fragment was indeed the PVY gene by sequencing analysis. PVY detection method using RT-PCR technique was about tuber tissue.