• Applied Biological Chemistry
• /
• v.30 no.1
• /
• pp.31-39
• /
• 1987

This study has been investigated the effect of Ganordema lucidum extract on Saccharomyces cerevisiae growth and physiology. Sacch. cerevisiae was inoculated in Hayduck solution medium which were added 0, 0.1, 0.5, 1.0% extracts of G. lucidum and fermented at $30^{\circ}C$ for 5 days respectively. Some results about cell number, alcohol content and carbon dioxide products during fermentation are as follows: $CO_2$ evolution of yeasts by addition of extract of G. lucidum was more increased than control after the fermentation for 120 hours. It was the most abundant by addition of 1.0% extract of pot-culture G. lucidum. The cell number of yeasts during the fermentation w as more increased than control by addition of extract of G. lucidum. It was by addition of extract of pot-culture G. lucidum that the cell number of yeasts was more increased than by each addition of extract of wood-culture G. lucidum and G. lucidum. Dry weight of yeasts was systematically increased in addition of extract of pot 0.5%>pot 1.0%>wild 1.0%>wood 1.0%=wood 0.5%>wild 0.5%>wild 0.1%>pot 0.1%>wood 0.1%>control in order. It was by addition of extract of pot-culture G. lucidum that. the dry weight of yeasts was more increased than by addition of woodculture G. lucidum and wild G. lucidum. Alcohol quantity by addition of extract of G. lucidum was increased more than 3 times after the fermentation for 72 hours compared with control but there was no any difference among them after the fermentation for 120 hours. The rate of sugar-consumption and fermentation of yeast by addition of extract of G. lucidum was highly increased during the early fermentation. As times went, there was no difference among them during the subsequent fermentation.

• Journal of Mushroom
• /
• v.3 no.1
• /
• pp.24-30
• /
• 2005

To study the cultural characteristics and wood rotting ability of the secondary mycellia of Armillaria mellea, it was cultivated on the various media. The optimal mycelial growth condition was 20~27 and pH 5.0~6.5 on PDB. A. mellea grew well on MEA, PDA and GP. Lactose and mannitol as carbon sources and glutamic acid as nitrogen sources were found to be effective as additives. A. mellea employed in this study have the characteristics of white rot types. Pine and oak wood were selected as candidates for sawdust substrate.

• 보존과학연구
• /
• s.30
• /
• pp.31-46
• /
• 2009

In the study, we confirmed morphological traits and calculated the Risk Class of 25 mould species, including Tyromyces palustris, through decomposition rate of substrate for characteristic confirmation of injured microorganisms. We received the injured microorganisms in wood and it was pure cultivated. We observed growth traits and decomposition rate of substrate. Also, it allows the calculation of Risk Class to be worried in the case of growth in wooden cultural properties. As a result, Tyromyces palustris, representative of brown-rot, has the characteristics about decomposition rate of substrate - Cellulose ; more active(+++), Lignin ; more active(+++), Xylan ; more active(+++), Starch ; active(++). We classified Tyromcyes palustris as a first Risk Class and as a second Risk Class about Schizophyllum commune et al(9 species, See table 1. We look forward to offering standard data base for evaluating Risk Class as a matter of applying preventive conservation in wooden cultural properties through our study.

• Journal of Mushroom
• /
• v.11 no.2
• /
• pp.87-91
• /
• 2013

When No. 42 strain of Pleurotus ostreatus was cultivated at five different media, MnP and Lac but no LiP activity was detected throughout the culture period in the media. The production of MnP and Lac by No. 42 strain of Pleurotus ostreatus were correlated with wheat bran composition in the medium. In the liquid culture, maximum production of MnP and Lac were observed in the medium contained glucose-peptone- yeast-wheat bran(GPYW). However, in solid medium, maximum production of MnP was observed in wood meal-wheat bran(WMW) medium, but that of Lac was observed in wheat bran(W) medium.

• Journal of Ginseng Research
• /
• v.30 no.3
• /
• pp.172-180
• /
• 2006

The purpose of this study is classifying types of American ginseng and estimating their production cost and revenue by the types. Usually, the American ginseng can be classified as 4 different types; wild ginseng(WG), wild simulated ginseng(WSG), woods grown ginseng(WGG), and field cultivated ginseng(FCG). This paper estimates costs and benefits for FCG, WGG, and WSG per acre. The WGG & WSG are produced under the tree at mountain while the FCG is produced at large scale farm with machinery. Annual profit for the FCG is $2,222 while that of the WGG and the WSG are $2,759 and $3,799 per acre. Although quantity produced per acre for the WGG and WSG(600lbs and 160lbs) are much smaller than that of the FCG(3,000lbs), prices per pound for the WGG and WSG($125, 375$) are higher than that of the FCG($24). In addition, production costs for the WGG and WSG are lower than that of the FCG because of the costs for seeds, shadow facility, and chemicals are different by the types of production.

• The Korean Journal of Mycology
• /
• v.49 no.4
• /
• pp.433-440
• /
• 2021

The fungal genus Trametes is globally distributed and comprises various wood-decay species, including the well-known medicinal mushroom Trametes versicolor, a popular remedy in traditional Asian medicine. Trametes species produce antioxidants, which have a wide range of health benefits. The pressent study evaluated seven indigenous Trametes species from Korea, which were cultivated in three different media (dextrose-yeast extract, DY; malt extract-yeast extract, MY; malt extract broth, MEB) and tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. We found that the medium consumption rate did not significantly differ between the media and among the strains (72-76%). However, the T. versicolor strains had a relatively low consumption rate (14-65%). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) tests demonstrated that culture filtrates of T. cf. junipericola, T. orientalis, T. suaveolens, and T. versicolor possessed antioxidant activity against damage from free radicals. In particular, T. cf. junipericola (DY) and T. versicolor (MY) had >80% activity in the DPPH and ABTS assays, compared with that of the positive control (ascorbic acid). Thus, our study identified promising candidates with substantial antioxidant activity among the indigenous strains of Trametes spp. from Korea.

• Journal of the Korean Society of Industry Convergence
• /
• v.24 no.3
• /
• pp.271-281
• /
• 2021

Pinus densiflora and Larix kaempferi forests not only extended the plantation management area but also cultivated to the sustainable wood supply chain during the last 5-years. These prescriptions are primarily done by manual operation activities, particularly in young tree tending. Two types of tending activities, brashing by brush cutter(BB) and pruning by pruning shear and saw(PP), were selected for the investigate of musculoskeletal disorders' level and postural risks using Ovako Working Posture Analysis System (OWAS). The postural risk indexes (BB and PP) ranged from 115 to 125 and 102 to 105, respectively. There is no significant difference between the species. The young tree tending operations had low postural risks. The results may be used as basic data to develop technical guideline for safe young trees.

• Journal of Sasang Constitutional Medicine
• /
• v.18 no.1
• /
• pp.22-29
• /
• 2006

1. Objectives and Methods The original insect of $Q\'{\i}C\'{a}o$ is described as the larvae of Holotrichia diomphalia Bates (Scarabaeidae) in oriental medicine literatures. Traditionally $Q\'{\i}C\'{a}o$ inhabit on rotting wood, compost, and organic debris of soil or thatched roofs. They have the very important distinguishing mark that wriggle along on their back through rotting wood, compost etc. and they never do inflict the living crops. but, Holotrichia diomphalia Bates as the $Q\'{\i}C\'{a}o$ is not right because of many questions, such as Holotrichia are the harmful insects eat into farm products, and those don't match well with korean farmers' moods if their cultivated lands are turned over for collecting $Q\'{\i}C\'{a}o$ larvae, and especially they don't go on using their back. 2. Results and Conclusions Accordingly, in the conclusion of this subject it is reported that the original insect of $Q\'{\i}C\'{a}o$ is the larvae of Korean Cetoniidae(Coleoptera; Scarabaeoidea) insects including Genus Protaetia Burmeister(Protaetia orientalis submarmorea Burmeister, P.brevitarsis seulensis Kolbe, etc.) and the larvae of Korean Cetoniinae insects. Also, those were the original larvae of $Q\'{\i}C\'{a}o$ that were applied to not only 'DongEuiBoGam' but also 'Dongyi Suse Bowon prescriptions'. Those feed on rotting wood, compost, and organic debris of soil or thatched roofs.

• Korean Journal of Medicinal Crop Science
• /
• v.14 no.2
• /
• pp.63-69
• /
• 2006

The methanolic (MeOH) extract of A. fruticosa bark, which showed immune-regulatory activities, was separated to purify an active compared by means of a multi-stage column chromatography. This resulted in the isolation and characterization of an isoflavone glycoside named 4', $6-Dimethoxyisoflavone-7-O-{\beta}-D-glucopyranoside$. Immuno-regulatory activities of the crude extract of Amorpha fruticosa LINNE bark were compared with that of an isoflavone glycoside (4', $6-dimethoxyisoflavone-7-O-{\beta}-D-glucopyranoside$). The crude methanolic extract of A. fruticosa and purified single compound showed 16% of relatively low cytotoxicity at a maximum concentration of 1.0 g/L in cultivated normal human lung cell line (HEL299). Cell growth of human T cells was increased up to 15%, 0.5 g/L of the crude extract added group. This was higher than a single compound added one. On the other hand, specific production rates of IL-6 and $TNF-{\alpha}$ from T cell were higher in the purified compound treat group ($0.82{\times}10^{-4}\;pg/cell$ and $1.08{\times}10^{-4}\;pg/cell$, respectively), compared to 0.5 g/L of the crude extract added group ($0.65{\times}10^{-4}\;pg/cell$ and $0.84{\times}10^{-4}\;pg/cell$, respectively). In addition, the growth of NK-92MI cells incubated with the crude extract was higher up to 56% over the cells grown with a single compound (0.5 g/L). In overall, the crude extract showed relatively higher immuno-regulatory activities compared with a single compound, probably due to the synergic effect given by other substances existed in the crude extract. Even though the siolated compound stimulated higher secretion of cytokines from human T cells.

• The Korean Journal of Mycology
• /
• v.46 no.1
• /
• pp.34-42
• /
• 2018

Tricholoma matsutake (Pine mushroom) is expensive, and its artificial cultivation has been tried in several countries. Until date, the only successful cultivation of artificial pine mushroom in pine forests uses matsutake-infected pine trees. The National Institute of Forest Science in Korea has been restudying this method since 2000. Success in fruit production and reproduction was achieved in 2010 and 2017, respectively, in the same locale. The successes proved that pine mushrooms could be cultivated artificially in the field using matsutake-infected pine trees. The fruiting of pine mushroom in October 2010 occurred 6 years, 6 months after the transplantation of matsutake-infected pine trees. Five pine mushrooms reoccurred in September 2017, 13 years, 5 months and 15 years, 5 months after the transplantation of the respective matsutake-infected pine trees. The distance between the matsutake-infected pine tree and the pine mushrooms was 12 cm at 6.6 years, 90~115 cm at 13.5 years, and 95 cm at 15.5 years. Fruiting bodies occurred 13~16 days after the underground temperature declined to below $19^{\circ}C$. In conclusion, the use of matsutake-infected pine trees remains the only way to artificially cultivate pine mushrooms. More knowledge of the environmental factors affecting matsutake fruiting would be beneficial.