• Korean Journal of Environmental Agriculture
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• v.32 no.2
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• pp.95-101
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• 2013

BACKGROUND: Rice (Oryza sativa) is the most important staple food of over half the world's population. This study was conducted to evaluate the possible impact of transgenic rice cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere of GM and non-GM rice cultivation soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with GM and non-GM rice were similar to each other, and there was no significant difference between GM and non-GM rice. Dominant bacterial phyla in the rhizosphere soils cultivated with GM and non-GM rice were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in GM and non-GM rice cultivated soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed similar patterns, but didn't show significant difference to each other. DNAs were isolated from soils cultivating GM and non-GM rice and analyzed for persistence of inserted gene in the soil by using PCR. The PCR analysis revealed that there were no amplified protox gene in soil DNA. CONCLUSION(S): These data suggest that transgenic rice does not have a significant impact on soil microbial communities, although continued research may be necessary.

• Korean Journal of Environmental Agriculture
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• v.21 no.2
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• pp.156-161
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• 2002

Two different cDNA clones for superoxide dismutase (SOD) were isolated from an weedy rice variety (Oryza sativa, cv. Bhutan14Ad) and were introduced into a cultivated rice variety (Oryza sativa, cv. Nakdong) in order to develop the environmental stress-resistant rice plants. Sequence analysis of the cloned cDNAS indicated that the deduced amino acid sequence of SOD-A is 88.4% identical to that of SOD-B. Furthermore, the nucleotide sequence of SOD-A is 99.3% identical to that of a Cu/Zn SOD gene of Oryza sativa (GenBank accession No. L36320). The nueleotide sequence of SOD-B was identical to that of the previously published SOD gene (Accession No. D01000). A cultivated rice variety, Nakdong-byeo, was transformed with chimeric SOD genes containing a actin promoter of rice and pin2 terminator using a particle bombardment technique. Transformed calli were selected on an selection medium containing phosphinothricin (PPT). Transgenic rice plants were regenerated from the PPT-resistant calli. PCR analysis with genomic DNAs from transgenic plants revealed that transgenes are introduced into rice genome.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.254-258
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• 1998

Distribution and occurrence of weedy rice in Kyonggi region were surveyed in 1996. Weedy rice was observed in 1368 fields (54.9%) of total 2490 fields. Almost two thirds of paddy fields in northern mountainous region were contaminated by weedy rice and more severe contamination, three forths of paddy fields, was observed in suburban regions. In those regions, occurrence of weedy rice was greater than those in north-eastern inland and south-western plain regions. The occurrence of weedy rice was higher in water seeding cultivation (66.7%) than other cultivation methods. The number of weedy rice per 10a was 756.7 plants in direct seeding on dry paddy and 379.4 plants in water seeding. The occurrence of weedy rice was higher in fields planted by farmer's seeds than that of paddy fields cultivated by certified seeds, and the longer the farmer's seeds being used, the more weedy rice occurred in paddy field.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.3
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• pp.218-222
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• 1982

It has been ascertained by a few researchers that soil salinities of the saline paddy fields have some effects upon the root development of the rice plants. But much is not known about the root formations of the rice plants in different varieties cultivated at the saline paddy field. Therefore this experiments were conducted to study the salinity tolerance of rice plants via variety test. Tested varieties were Akibare, Jinheung, Milyang #30, Josaeng Tongil, Suweon #264 and Suweon #287. And testing methods were cultivation experiment at the saline paddy field and leaf-cutting method at the laboratory. The results of cultivation experiment showed Milyang #30 and Josaeng Tongil were superior to others in root formation, and those of leaf-cutting method revealed that Josaeng Tongil and Mi1yang #30 were also superior to others in root formation and root length.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.829-835
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• 2012

The cultivation of genetically modified (GM) crops has increased due to their economic and agronomic advantages. Before commercialization of GM crops, however, we must assess the potential risks of GM crops on human health and environment. The aim of this study was to investigate the possible impact of Bt rice on the soil microbial community. Microbial communities were isolated from the rhizosphere soil cultivated with Bt rice and Nakdong, parental cultivar and were subjected to be analyzed using both culture-dependent and molecular methods. The total counts of bacteria, fungi, and actinomycetes in the rhizosphere of transgenic and conventional rice were not significantly different. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that the bacterial community structures during cultural periods were very similar each other. Analysis of dominant isolates in the rhizosphere cultivated with Bt and Nakdong rice showed that the dominant isolates from the soil of Bt rice and Nakdong belonged to the Proteobacteria, Cloroflexi, Actinobacteria, Firmicutes, and Acidobacteria. These results indicate that the Bt rice has no significant impact on the soil microbial communities during cultivation period. Further study remains to be investigated whether the residue of Bt rice effect on the soil environment.

• Journal of Food Hygiene and Safety
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• v.25 no.2
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• pp.143-147
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• 2010

Chronic exposure to Arsenic (As) causes significant human health effects including various cancers. Total As concentrations from 300 polished rice samples cultivated near the mining areas in Korea were analyzed to estimate a probabilistic assessment of human health risk from As-contaminated rice. The mean of total As concentrations in rice was 0.09 mg/kg and lognormal distribution model was set for total As concentrations. Human health risk for As in rice was estimated using gender-specific rice consumption data and average daily dose (ADD). While cancer risk (CR) and hazard quotient (HQ) were calculated using oral cancer slope factor (OCSF) and Reference dose (RfD) suggested by the U.S. EPA. Mean of CR posed by total As was 2.16 (for male) and 1.83 (for female) per 10,000. The HQ for general population from rice cultivated near the mining areas in Korea was below 1 as the $50^{th}$ percentile of general population. However, less than 10% of general population consuming rice cultivated near the mining areas would exceed 1.0. This result is similar with those from each gender-specific group.

• Journal of Soil and Groundwater Environment
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• v.22 no.6
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• pp.12-21
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• 2017

A pot experiment was conducted to investigate the transfer of As and cationic heavy metals (Fe, Mn, Zn, Cd and Pb) from soil to rice plant in soil condition with submerged and drained. During the ninety-day monitoring period for soil solution, solubility of reducible elements such as As, Fe and Mn in submerged condition were higher than that of Zn. On the contrary, concentration of Zn in drained condition was higher than that of reducible elements. The concentration of As, Cd, Pb and Zn in rice plant (root, stem, leaf and grain) showed similar pattern with soil solution. The As concentration in each part of rice plant, which cultivated in drained condition, measured 56%~94% lower than those in submerged condition. However, the contents of cationic heavy metals (Cd, Pb and Zn) were represented the opposite result with As. These results are due to mobility of As and cationic heavy metals under different soil drainage conditions which represent oxidation and reduction. Thus soil drainage control can be used as acceptable passive treatment methods to reduce transfer of inorganic contaminants from soil to rice plant. However more detailed examination on soil condition conversion is needed, because yield of rice was decreased when it cultivated in drained condition only. It also needed when soil is contaminated by As and cationic heavy metal because single drainage condition cannot reduce transfer of both kinds of contaminants all.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.71-78
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• 1969

To study the varietal differences of vegetative organs, yield and yield components along the periodical changes of rice variety in korea, this experiment was conducted in 1968. Eighteen used varieties were grouped into 6 varietal groups by the cultivated period as follow.: 1) Group I - Korean local varieties cultivated about 1907, 2) Group II - introduced varieties cultivated in 1907 - 1930, 3) Group III - introduced varieties cultivated in 1930-1960, 4) Group IV - improved varieties cultivated in 1930-1960, 5) Group V - introduced varieties cultivated after 1960 and 6) Group VI - improved varieties cultivated after 1960. 1. Varietal Group I showed the longest leaves, the largest leaf area, the lowest value of leaf area index and the highest value of flag leaf angle. Along the periodical changes of rice varieties the leaf length, the total leaf area and the flag leaf angle were decreased, and the leaf area index was increased. 2. Varietal Group I showed higher value of the length and the weight of leaf sheath than those of Group II, III, IV, V and VI. The length of every internode was longer in Group I than in the other varietal groups. The weight of every internode was shown higher value in Group 1. The length and the weight of every internode were decreased along the periodical changes of rice variety. 3. Varietal Group I and Group III showed the higher value of culm length, panicle length and the number of grain per panicle length and groups. Group IV, V and VI had the higher value of the number of panicle, the maturing rate and the 1, 000 grains weight. Varietal Group V and VI which are cultivated in recent years showed shorter culm and panicle length, higher value of the maturing rate and the 1, 000 grains weight, and intermediate in the number of panicle and the number of grain per panicle. 4. The lowest value of straw weight was found in Group I. The highest value of grain yield was shown in Group VI, intermediate in Group II, III, IV and V and lowest in Group I. Grain-straw ratio was shown highest in Group VI.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.479-484
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• 1999

Rice crop has relatively short growing season during the summer in Korea and, therefore, it is often difficult to acquire cloud-free imagery on time. This study was attempt to define the temporal characteristics of radar backscattering observed from satellite L-band SAR data on different growing stages of rice crop. Six scenes of multi-temporal JERS SAR data were obtained from the transplanting season to the harvesting month of October. Six layers of multi-temporal SAR data were registered on a common geographic coordinate system. Using topographic maps, field collected data, and Landsat TM data, several sample rice fields were delineated from the imagery and their relative radar backscatters were calculated by using a set of reference targets. The temporal pattern of radar backscattering was very distinctive by the growing stage of rice crop. It was also separable between two types of rice fields having different cultivation practices. Considering the temporal characteristics of radar backscattering observed from the study, it is obvious that a certain date of the growing season can be more effective to delineate the exact area of the cultivated rice crop field.

• Korean Journal of Agricultural Science
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• v.42 no.1
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• pp.1-5
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• 2015

Drought is one of the major abiotic constraints limiting quantity and quality of rice grain. In order to elucidate the effects of drought on chemical compositions of rice grain, seedlings were cultivated in a rainout shelter controlled with well-watered or water-deficit conditions. After harvesting, the key components including proximates, amino acids, fatty acids, minerals, and vitamins of rice grains were analyzed. Drought stress increased the amounts of methionine, phenylalanine, linoleic acid, linolenic acid, calcium, potassium, vitamin B1, and vitamin E in rice grains, whereas it decreased the contents of lignoceric acid. Particularly, drought stress caused a marked increase in vitamin E content. These results indicate drought significantly influence the chemical compositions of rice grains.