A hot water extract was prepared from the artificially grown Gastrodia elata to investigate its chemical composition and various in vitro biological activities as an effort to develop G. elata as health/functional food materials. The contents of crude protein, ash, fat, fiber, moisture and total sugar were 5.4, 2.6, 3.6, 3.3, 8.1 and 77% (w/w), respectively. The extract of G. elata had greater amount of potassium (1,150 mg/100 g) than phosphorus (300 mg/100 g). Dose-dependence against human carcinoma (Hep3B, MCF-7, A549 and AGS) were observed from 0.2 mg/ml to 1.0 mg/ml. Especially, the treatment of 1.0 mg/ml extracts showed the highest cytotoxicity with 83% against gastric carcinoma (AGS). The extracts showed weak antimicrobial activities against Bacillus subtilis and Pseudomonas aeruginosa, but practically no antimicrobial activity against the other microorganisms tested. The effect of ${\alpha}$-glucosidase inhibition was 64% at the concentration of 1.0 mg/ml. The inhibitory effect of angiotensin converting enzyme (ACE) of the extract in the range of $0.2{\sim}1.0mg/ml$ showed $63{\sim}89%$, and the highest ACE inhibition was 89% at the concentration of 1.0 mg/ml of extracts. The highest activity of glutathion S-transferase (GST) was 221 % at the concentration of 1.0 mg/ml of the G. elata extracts. These results suggest that G. elata may be used as health/functional food materials.
LEE Eung-Ho;CHUNG Seung-Yong;KIM Soo-Hyeun;RYU Byeong-Ho;HA Jin-Hwan;OH Hoo-Gyu;SUNG Nak-Ju;YANG Syng-Tack
Korean Journal of Fisheries and Aquatic Sciences
/
v.8
no.2
/
pp.90-100
/
1975
The estimation of the pre-processing condition of oyster is of great importance for distributors and processors. This study was attempted to establish the basic data for evaluating the processing suitability of oyster, which is the most important shellfish for domestic use and export. The data were analysed by measuring the condition index, chemical composition and heavy metal content of oysters. In order to eliminate the manual work that has to be done on a tightly closed oyster shell and avoid shrinkage in the oyster meat which is attendant on the steaming process, chemical means to open oyster were examined. finding the method of pretreatment of polyphosphate for frozen oysters were attempted to improve the product quality. The prevention of undesirable color change of the canned oyster meat is another problem to solve. The important results are as follows : 1. The ratio of meat volume and meat weight to the holding capacity by shells may be useful as an index to measure the condition index of oysters. 2. As a whole, monthly changes of moisture and fat content in oysters were reversely correlated. Protein content slightly decreased from April and rapidly decreased in July, and again rapidly increased in August but from September to November decreased slightly. In April, the content of glycogen was 4 percent. From this period to September, glycogen was rapidly decreased. From July to September, it was only 0. 7 to 1 percent but increased from October. There were little seasonal changes in pH value. The pH value of oyster meat was 6.0 to 6.2. The crude ash content was slightly decreased from June to August. 3. The range of monthly change of heavy metal content are as follows: Total mercury was 0 to 0.019 ppm, cadmium was 0.026 to 0.053 ppm, copper was 0.111 to 0.594 ppm, and lead_was 0.061 to 0.581 ppm. 4. By the results of condition index, chemical composition and heavy metal content of oysters, the suitable harvest season as raw materials for processing was the end of December to the end of May of next year. 5. The pretreatment of 10 percent polyphosphate in 5 percent salt solution of oyster meat appeared effective to reduce thawing drip during cold storage. 6. The pretreatment of $Na_2EDTA$ and BHA did not show the color prevention effect to the canned oyster meat during storage. 7. Magnesium chloride affected to open the valves of oysters.
PARK Yeung-Ho;LEE Eung-Ho;LEE Kang-Ho;PYEUN Jae-Hyeung;RYU Hong-Soo;CHOI Su-An;KIM Seun-Bong
Korean Journal of Fisheries and Aquatic Sciences
/
v.12
no.3
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pp.191-200
/
1979
For the use of antarctic krill as a fond protein source its compositional characteristics were investigated as the first part of the work includes other subjects such as processing of drill paste, concentrates, and fermented or seasoned product. In general composition of fresh frozen and preboiled frozen krill on board, the contents of crude fat and free amino nitrogen were higher in the former than in the latter which contained a high amount of ash. VBN was rather high as much as 37.6 and $26.4\;mg\%$ in both fresh frozen and preboiled krill. The pH of drill homogenates was 7.1 to 7.2 in both cases. Such a low pH might be attributed to a long term storage and temperature fluctuations during frequent transshipping. The amino acid competition of fresh frozen krill meat showed relatively high amount of glutamic acid, aspartic acid, lysine, proline, and leucine while methionine, histidine, serine, tyrosine, and phenylalanine were lower. Among the essential amino acids lysine and leucine were higher and methionine was lower. In tile composition of free amino acid proline, lysing, arginine, and alanine were higher comparatively to the contents of histidine, aspartic acid, serine, and threonine. It is noteworthy for nutritional qualification that tile essential amino acids particularly as lysine were abundant similarly to that of fishes. Heavy metal contents of krill meat 0.039 to 0.048 ppm as Hg, 0.06 to 0.11 ppm as Pb, less than 0.32 ppm as Zn, 0.008 to 0.012 ppm as Cd, 0.61 to 0.68 ppm as Fe, 0.87 to 1.37 ppm as Cu, and nondetective as Cr. A high Cu content seems to be resulted by tile blood pigment of crustacea. The ratio,1 of edible portion to non-edible portion were 37:63 in fresh frozen and 42:58 in preboiled frozen krill respectively. Release of drip after thawing was more in fresh frozen than in preboiled frozen drill marking $36\%$ and $24\%$ of both respectively.
A study was carried out to examine the effects of dietary kaolin on the performance, feed and nutrient efficiency, meat quality and feeding environment using 200 day-old growing ducts. Four replicates of 10 birds each was assigned to diets containing 0(C), 1 (T1), 2(T2), 3(T3) and $4(T4)\%$ of kaolin. Body weight gain in T1 and T2 were higher than in $C(p<0.05)$ whereas weight gain in T3 and T4 were not significantly different from the control. Feed intake and feed conversion ratio tended to increased as the level of dietary kaolin increased, but without significant differences among treatments. Mortality of growing ducks during 6 week period was not significantly different among treatments. Dressing percentage was highest in T1 and lowest in T4 $(p<0.05)$. The utilizability of nutrients except for crude fat Increased$(p<0.05)$, as the level of dietary kaolin increased. Moisture content and $NH_3$ gas emission of excreta was not significantly influenced by dietary kaolin. $H_2S$ gas emission of excreta in T2, T3 and T4 was lower compare to that in C and $T1(p<0.05)$. Cholesterol content in breast and thigh meat was not different among treatments. Values for pH, WHC, TBARS, POV and Cooking loss of breast and thigh meat were not significantly different or did not show any trend among treatments.
The purpose of this study was to monitor physicochemical changes of olive flounder (Paralichthys olivaceus) muscle by iced water pre-treatment. Moisture content, crude fat content, nucleotide content, texture (hardness and toughness), and rigor mortis were assessed. The sensory evaluation was performed with a nine-point hedonic test. K-values, a parameter of fish flesh freshness, were also calculated from the content of nucleotides and their corresponding decomposition products. Pre-treatment of flounder flesh with iced water was found to be fresher compared to the control, as determined by a difference in the K-values. Iced water pre-treatment hastened postmortem stiffness, as judged from the rigor index, and increased inosine monophosphate (IMP), which is known to be a savory taste compound, more quickly as adenosine triphosphate (ATP) degradation proceeded.
Kang, Geunho;Ham, Hyoung-Joo;Seong, Pil-Nam;Cho, Soohyun;Moon, Sungsil;Park, Kyoungmi;Kang, Sun Mun;Park, Beom-Young
Korean Journal of Poultry Science
/
v.41
no.2
/
pp.77-85
/
2014
This study was conducted to investigate the effect of duck and pork meat mixing ratio on quality characteristics of emulsion type sausage at $4^{\circ}C$ during 5 weeks. Treatments on the basis of the meat content were subjected to 100% duck meat (T1), 100% pork meat (T2), 50% duck meat + 50% pork meat (T3), 40% duck meat + 60% pork meat (T4), and 30% duck meat + 70% pork meat (T5). The moisture content was significantly (p<0.05) higher in TI sample but significantly (p<0.05) lower in T2 sample than those in comparison to the other treatments. Crude protein and fat content were significantly (p<0.05) higher in T2 sample compared to the other treatments. CIE $L^*$value was significantly (p<0.05) lower in T1 sample than those of other treatments until 5 weeks of cold storage. CIE $a^*$value was significantly (p<0.05) higher in T1 sample but significantly (p<0.05) lower in T2 sample than those in comparison to the other treatments until 5 weeks of cold storage. Hardness was significantly (p<0.05) higher in T4 sample compared to the other treatments during all cold storage. Cohesiveness was significantly (p<0.05) higher in T5 sample compared to the other treatments until 2 weeks of cold storage. The results of sensory evaluation showed that the meat flavor, taste and texture were significantly (p<0.05) lower in T5 sample compared to the other treatments whereas no difference among treatments except T5 sample. Overall acceptability was significantly (p<0.05) lower in T5 sample compared to the other treatments. Therefore, these results suggested that the ratio of 40% duck meat and 60% pork meat is appropriate levels for hardness and palatability when manufacturing emulsion type sausage with duck meat.
The composition of fatty acids in bakery products from 35 bakeries in Seoul was analyzed by GCFID. The contents of crude fat in bakery products were represented 9.54-44.30% in pastries, 14.67-41.22% in cookies and 7.63-28.15% in whipped cream cakes. The total saturated fatty acid content in pastries, cookies and whipped cream cakes were 12.8-75.1 %, 8.7-81.6% and 50.2-85.9% respectively. The proportion of trans fatty acids in pastries ranged from 0.00 to 3.37%. Cookies and whipped cream cakes were represented varying amount of trans fatty acid with highest value of 4.55% in cookies and 2.13% in whipped cream cakes. Total estimated daily intakes of fatty acids from pastries and whipped cream cakes were as follows: 1.2 g/day/person for saturated fatty acids and 0.014 g/day/person for trans fatty acids.
This experiment was conducted to determine the effects of dietary copper levels on performance, carcass characteristics, and economical analysis in Hanwoo steers. Fifteen Hanwoo steers weighing about 300kg were randomly allotted into one of three treatments. The three treatments consisted of Control(7mg Cu/kg feed), TI(12mg Cu/kg feed) and TII(17mg Cu/kg feed). Copper was formulated with concentrate from copper sulfate($CuSO_4$) and animals were fed the diets supplemented with copper from 300kg untill about 630 kg of body weight. Dietary copper levels did not affect daily weight gain, feed intake and feed efficiency during the whole feeding period. Dressing percentage, rib eye area and backfat thickness were not different between animals fed the diets supplemented with copper. Animals fed the diets supplemented with copper received the same B grade in yield grade. Animals fed the diets supplemented with copper received same marbling score. Beef color, fat color, texture and maturity were not affected by Cu supplementation in diet. Animals received the same first grade between treatments in quality grade. Animals fed the diet supplemented with 12mg of copper per kg diet was higher in profit by 13.3% than animals fed the control diet. According to these results, it may be concluded that performance, yield and quality grade were not affected by the Cu levels of 7 to 17mg per kg feed but crude income was enhanced when Hanwoo steers were fed the diet containing 12mg Cu/kg feed.
This study was conducted to investigate the effect of single or mixed supplementation of bacterial and fungal phytase using 45-wk-old 450 Hy-Line Brown laying hens housed in individual cages for 12-wk period. The birds were reallocated to have similar egg productivity by examining the egg production for one wk before starting the experiment. Two sources of phytase, bacterial (BP) and fungal (FP), were used either in single or mixture to determine the effects of these phytase. Five dietary treatments consisted of control (BP 0, FP 0), T1 (BP 300, FP 0), T2 (BP 300, FP 300), T3 (BP 300, FP 3000), and T4 (BP 0, FP 3000). The DPU was used for phytase activity in this experiment. The nonphytate phosphorus (NPP) content of control was 0.30%, and those of phytase treatments were set to 60% of the Control. Experimental diets were fed ad libitum throughout the experimental period. The lighting schedule of 17L7D was employed. The egg production was not different between control and bacterial phytase treatments, but the T4 showed significantly low productivity compared to control (P<0.05). No difference was found in average egg weight among all treatments. The daily egg mass did not show any statistical differences among all treatments: however, it was significantly low in T4 compared to ther control during the latter half of the experiment (P<0.05). No significant difference was found among treatments in terms of feed intake, feed conversion and egg quality. The digestibilities of dry matter, crude protein, and fat digestibility were similar regardless of the treatments. No significant trends were detected in Ca and P availability. In conclusion, the BP level of 300 DPU contributed to achieve 40% reduction of recommended nonphytate phosphorus addition. The synergistic effect of bacterial and fungal phytase was not confirmed.
The experiment was conducted to find out the nutritive value and microbial status of different wholesale cuts of goat carcass. The meat sample (4 cuts from each animal $\times$ 3 different age groups $\times$ 3 animals in each group = 36 samples) was obtained from 1-, 2-, and 3-year aged goats. The whole sale cuts were shoulder, rack, loin and leg of each goat carcass. To assess the quality of meat sample, the general appearance, color, smell, juiciness, proximate composition, pH, total bacteria, coliform bacteria, and yeast were studied. The mean pH value of different cuts ranges from 5.65-5.69 didn't differ significantly, but due to age differences the pH values (5.59-5.74) differed significantly (p < 0.01). The values of juiciness in different ages ranged from 32.24-42.10% which differed significantly (p < 0.01). The marbling of the cuts of rack portion was more pronounced than that of other cuts. The ranges of crude protein (CP) content of goat carcass (20.78-27.71%) differed significantly (p < 0.01) and leg portion contained higher CP than other portion. Fat contents of different cuts ranged from 2.66-11.47% differed significantly (P < 0.01). The moisture content of the carcass differed significantly which ranged from 69.20-73.31%. The ash content of the cuts of 1-year aged groups (0.99 $\pm$ 0.13%) was higher than that of other age groups and differed significantly (P < 0.01). The calcium (Ca) content did not differ significantly. The phosphorus (P) content was higher in one year old goat (0.15 $\pm$ 0.03%) than that of the goats of other ages. The total viable count (TVC) content of microorganisms ranging from 5.05-5.15 log cfu/g at different ages did not differ significantly. The coliform count (CC) of different cuts differed significantly (P < 0.01) which ranged from 2.56-3.05 log cfu/g; it also differed significantly (P < 0.05) in different ages (2.79-2.84 log cfu/g) and was higher in 1 year old goat carcass. The yeast count differed significantly in different cuts (P < 0.01) and ages (P < 0.05). From the study it is concluded that the age and different wholesale cuts have direct influence on quality of goat carcass.
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