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http://dx.doi.org/10.4489/KJM.2002.30.2.136

A Studies on the Chemical Composition and in vitro Biological Activities of a Hot Water Extracts of Gastrodia elata  

Kang, Tae-Su (Department of Food Science and Biotechnology, Chungbuk Provincial University of Science and Technology)
Kong, Young-Jun (Regional Crop Development Experiment Station, Kangwon Provincial Agricultural Research and Extention Services)
Kwon, Hye-Jeong (Department of Food Science and Biotechnology, Chungbuk Provincial University of Science and Technology)
Choi, Byoung-Kon (Department of Food Science and Biotechnology, Chungbuk Provincial University of Science and Technology)
Hong, Jung-Gi (Department of Food Science and Biotechnology, Chungbuk Provincial University of Science and Technology)
Park, Yong-Kil (Chonma Industrial Co.)
Publication Information
The Korean Journal of Mycology / v.30, no.2, 2002 , pp. 136-141 More about this Journal
Abstract
A hot water extract was prepared from the artificially grown Gastrodia elata to investigate its chemical composition and various in vitro biological activities as an effort to develop G. elata as health/functional food materials. The contents of crude protein, ash, fat, fiber, moisture and total sugar were 5.4, 2.6, 3.6, 3.3, 8.1 and 77% (w/w), respectively. The extract of G. elata had greater amount of potassium (1,150 mg/100 g) than phosphorus (300 mg/100 g). Dose-dependence against human carcinoma (Hep3B, MCF-7, A549 and AGS) were observed from 0.2 mg/ml to 1.0 mg/ml. Especially, the treatment of 1.0 mg/ml extracts showed the highest cytotoxicity with 83% against gastric carcinoma (AGS). The extracts showed weak antimicrobial activities against Bacillus subtilis and Pseudomonas aeruginosa, but practically no antimicrobial activity against the other microorganisms tested. The effect of ${\alpha}$-glucosidase inhibition was 64% at the concentration of 1.0 mg/ml. The inhibitory effect of angiotensin converting enzyme (ACE) of the extract in the range of $0.2{\sim}1.0mg/ml$ showed $63{\sim}89%$, and the highest ACE inhibition was 89% at the concentration of 1.0 mg/ml of extracts. The highest activity of glutathion S-transferase (GST) was 221 % at the concentration of 1.0 mg/ml of the G. elata extracts. These results suggest that G. elata may be used as health/functional food materials.
Keywords
Biological activity; Composition; Gastrodia elata; In vitro;
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