• Title/Summary/Keyword: crtI

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Molecular Cloning and Co-Expression of Phytoene Synthase Gene from Kocuria gwangalliensis in Escherichia coli

  • Seo, Yong Bae;Choi, Seong-Seok;Lee, Jong Kyu;Kim, Nan-Hee;Choi, Mi Jin;Kim, Jong-Myoung;Jeong, Tae Hyug;Nam, Soo-Wan;Lim, Han Kyu;Kim, Gun-Do
    • Journal of Microbiology and Biotechnology
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    • v.25 no.11
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    • pp.1801-1809
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    • 2015
  • A phytoene synthase gene, crtB, was isolated from Kocuria gwangalliensis. The crtB with 1,092 bp full-length has a coding sequence of 948 bp and encodes a 316-amino-acids protein. The deduced amino acid sequence showed a 70.9% identity with a putative phytoene synthase from K. rhizophila. An expression plasmid, pCcrtB, containing the crtB gene was constructed, and E. coli cells containing this plasmid produced the recombinant protein of approximately 34kDa , corresponding to the molecular mass of phytoene synthase. Biosynthesis of lycopene was confirmed when the plasmid pCcrtB was co-transformed into E. coli containing pRScrtEI carrying the crtE and crtI genes encoding lycopene biosynthetic pathway enzymes. The results obtained from this study will provide a base of knowledge about the phytoene synthase of K. gwangalliensis and can be applied to the production of carotenoids in a non-carotenoidproducing host.

Carbohydrate Antigen 19-9 Levels Associated with Pathological Responses to Preoperative Chemoradiotherapy in Rectal Cancer

  • Yeo, Seung-Gu;Kim, Dae Yong;Kim, Tae Hyun;Kim, Sun Young;Baek, Ji Yeon;Chang, Hee Jin;Park, Ji Won;Oh, Jae Hwan
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.13
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    • pp.5383-5387
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    • 2014
  • Purpose: To investigate whether pretreatment serum carbohydrate antigen 19-9 (CA 19-9) levels are associated with pathological responses to preoperative chemoradiotherapy (CRT) in patients with rectal cancer. Materials and Methods: In total, 260 patients with locally advanced rectal cancer (cT3-4NanyM0) who underwent preoperative CRT and radical surgery were analyzed retrospectively. CRT consisted of 50.4 Gy pelvic radiotherapy and concurrent chemotherapy. Radical surgery was performed at a median of 7 weeks after CRT completion. Pathological CRT response criteria included downstaging (ypStage 0-I) and ypT0-1. A discrimination threshold of CA 19-9 level was determined using a receiver operating characteristics analysis. Results: The median CA 19-9 level was 8.0 (1.0-648.0) U/mL. Downstaging occurred in 94 (36.2%) patients and ypT0-1 in 50 (19.2%). The calculated optimal threshold CA 19-9 level was 10.2 U/mL for downstaging and 9.0 U/mL for ypT0-1. On multivariate analysis, CA 19-9 (${\leq}9.0U/mL$) was significantly associated with downstaging (odds ratio, 2.089; 95% confidence interval, 1.189-3.669; P=0.010) or ypT0-1 (OR, 2.207; 95%CI, 1.079-4.512; P=0.030), independent of clinical stage or carcinoembryonic antigen. Conclusions: This study firstly showed a significant association of pretreatment serum CA 19-9 levels with pathological CRT responses of rectal cancer. The CA 19-9 level is suggested to be valuable in predicting CRT responses of rectal cancer cases before treatment.

Enhanced Production of Astaxanthin by Metabolic Engineered Isoprenoid Pathway in Escherichia coli (대장균에서 이소프레노이드 생합성 경로의 대사공학적 개량에 의한 아스타잔틴의 생산성 향상)

  • Lee, Jae-Hyung;Seo, Yong-Bae;Kim, Young-Tae
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1764-1770
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    • 2008
  • The goal of this study is to increase production of astaxanthin in recombinant Escherichia coli by engineered isoprenoid pathway. We have previously reported structural and functional analysis of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis. The carotenoid biosynthesis gene cluster involved in astaxanthin production contained six carotenogenic genes (crtW, crtZ, crtY, crtI, crtB, and crtE genes) and recombinant E. coli harboring six carotenogenic genes from P. haeundaensis produced 400 ${\mu}g$/g dry cell weight (DCW) of astaxanthin. In order to increase production of astaxanthin in recombinant E. coli, we have cloned 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (lytB), farnesyl diphosphate (FPP) synthase (ispA), and isopentenyl (IPP) diphossphate isomerase (idi) in the isoprenoid pathway from E. coli and coexpressed these genes in recombinant E. coli harboring the astaxanthin biosynthesis genes. This engineered E. coli strain containing both isoprenoid pathway gene and astaxanthin biosynthesis gene cluster produced 1,200 ${\mu}g$/g DCW of astaxanthin, resulting 3-fold increased production of astaxanthin.

Metabolic Engineering of Deinococcus radiodurans for the Production of Phytoene

  • Jeong, Sun-Wook;Kang, Chang Keun;Choi, Yong Jun
    • Journal of Microbiology and Biotechnology
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    • v.28 no.10
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    • pp.1691-1699
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    • 2018
  • A metabolically-engineered Deinococcus radiodurans R1 strain capable of producing phytoene, a colorless $C_{40}$ carotenoid and a promising antioxidant, has been developed. To make this base strain, first, the crtI gene encoding phytoene desaturase was deleted to block the conversion of phytoene to other carotenoids such as lycopene and ${\gamma}$-carotene. This engineered strain produced $0.413{\pm}0.023mg/l$ of phytoene from 10 g/l of fructose. Further enhanced production of phytoene up to $4.46{\pm}0.19mg/l$ was achieved by overexpressing the crtB gene encoding phytoene synthase and the dxs genes encoding 1-deoxy-$\text\tiny{D}$-xylulose-5-phosphate synthase gene, and by deleting the crtD gene. High cell-density culture of our final engineered strain allowed production of $10.3{\pm}0.85mg/l$ of phytoene with the yield and productivity of $1.04{\pm}0.05mg/g$ and $0.143{\pm}0.012mg/l/h$, respectively, from 10 g/l of fructose. Furthermore, the antioxidant potential of phytoene produced by the final engineered strain was confirmed by in vitro DPPH radical-scavenging assay.

Theoretical Analysis of the High Voltage Generator System for CRT Displays (CRT 디스플레이용 고전압 발생기 시스템의 이론적해석)

  • 지철근;박지식
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.36 no.11
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    • pp.826-835
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    • 1987
  • In this paper, the horizontal output and flyback transformer (FBT) stages during the retrace time are represented by a new simplified equivalent circuit. The circuit analysis is performed by means of this equivalent circuit and the computed results are compared with those of experiment The results show that for fixed other circuit parameters, the high voltage regulation depends upon the coupling factor of transformer, the V-I characteristics and the charge storage of the high voltage rectifying diodes. The computed output voltages are found to be about 3.9 to 5.8% higher than the measurements and these are probably due to the neglection of the transformer losses. These acceptable errors show that the newly suggested equivalent circuit is reasonable.

Evaluation of Various Escherichia coli Strains for Enhanced Lycopene Production

  • Jun Ren;Junhao Shen;Thi Duc Thai;Min-gyun Kim;Seung Ho Lee;Wonseop Lim;Dokyun Na
    • Journal of Microbiology and Biotechnology
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    • v.33 no.7
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    • pp.973-979
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    • 2023
  • Lycopene is a carotenoid widely used as a food and feed supplement due to its antioxidant, anti-inflammatory, and anti-cancer functions. Various metabolic engineering strategies have been implemented for high lycopene production in Escherichia coli, and for this purpose it was essential to select and develop an E. coli strain with the highest potency. In this study, we evaluated 16 E. coli strains to determine the best lycopene production host by introducing a lycopene biosynthetic pathway (crtE, crtB, and crtI genes cloned from Deinococcus wulumuqiensis R12 and dxs, dxr, ispA, and idi genes cloned from E. coli). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic engineering, and further, that MG1655 is a potent host for producing lycopene and other carotenoids with the same lycopene biosynthetic pathway.

Design and testing large FOV retinal displays on the basis holographic combiner

  • Gan, Mikhail A.;Gan, Iacov M.;Tchertkov, Alexander S.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.442-445
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    • 2004
  • We report principles and results of design large FOV retinal display systems by software WinDEMOS and software for computer testing display systems TEDiS. We discuss results design head mounted and head up display. As combiner we are used volume interference recorded or synthetic HOE, and as the sours of the image high resolution transmitting or reflective liquid crystal matrixes on silicon (LCOS) or CRT.

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The Anti-inflammatory Effect of Cheongyeonsan for Allergic Rhinitis (알러지성 비염에 대한 청연산 추출물의 항염증효과)

  • Kim, Jeong Won;Ahn, Sang Hyun;Kim, Ki Bong
    • The Journal of Pediatrics of Korean Medicine
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    • v.32 no.1
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    • pp.19-29
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    • 2018
  • Objectives This study investigated the effects of Cheongyeonsan for allergic rhinitis. Methods First, the GS/MS was used to analyze the effects of Cheongyeonsan by measuring inflammatory markers. Second, 3 groups of 10 6-week-old BALB/c mice were divided into Ctrl (no treatment), ARE (allergic rhinitis-induced without treatment), and CRT (allergic rhinitis-induced after Cheongyeonsan treatment) groups. Ovalbumin (OVA) was used as an antigen to induce allergic rhinitis and sensitization was performed by intraperitoneal injection of 0.1% OVA solution 21, 14, and 7 days before the onset of allergic rhinitis. Allergic rhinitis was induced by dropping OVA solution on the nasal cavity of each mouse for 5 days after the last sensitization. Seven days after the first induction, second induction was introduced by the same method. After making the section, MMP-9, substance P, $TNF-{\alpha}$, $NF-{\kappa}B$ p65, COX-2, iNOS and Nrf, apoptotic cells were observed by Masson trichrome staining, immunohistochemical staining, TUNEL of nasal mucosal tissues of each group. Results GC/MS results showed undecanoic acid. Masson trichrome results showed that the CRT group had less respiratory epithelial damage. Immunohistochemical staining showed CRT group had 58% decrease in MMP-9, 61% decrease in substance P, 55% decrease in $TNF-{\alpha}$, 38% decrease in $NF-{\kappa}B$ p65, 53% decrease in COX-2, 54% decrease in iNOS, 87% increase in Nrf compared to those of the ARE group. TUNEL showed a positive reaction of 84% increase in apoptotic cells greater than that of the ARE group. Conclusions Cheongyeonsan alleviates nasal mucosal damage and reduces inflammatory mediators from allergic rhinitis-induced mice.

A Development of Distributed Parallel Processing algorithm for Power Flow analysis (유기EL과 무기EL에 관한 연구)

  • Lee, Han-Sung
    • Proceedings of the KIEE Conference
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    • 2001.07e
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    • pp.67-71
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    • 2001
  • Nowdays, we can communicate using Information Technology such as internet, personal computer, mobile phone etc. To protect global environment, it is also reqired to invent products efficiently reduce energy consumption. here, I studied organic EL and inorganic EL because organic EL display is appropriate device as light, thin, energy-saying display following CRT, LCD. As a result, I realized that we are supposed to study more on invention, efficiency and mass-production of materials. Comparing with another display, however, it would be marketable in few years, considering short history of its full-scaled studies.

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