Browse > Article
http://dx.doi.org/10.5352/JLS.2008.18.12.1764

Enhanced Production of Astaxanthin by Metabolic Engineered Isoprenoid Pathway in Escherichia coli  

Lee, Jae-Hyung (Basic Science Research Institute, Pukyong National University)
Seo, Yong-Bae (Department of Microbiology, Pukyong National University)
Kim, Young-Tae (Department of Microbiology, Pukyong National University)
Publication Information
Journal of Life Science / v.18, no.12, 2008 , pp. 1764-1770 More about this Journal
Abstract
The goal of this study is to increase production of astaxanthin in recombinant Escherichia coli by engineered isoprenoid pathway. We have previously reported structural and functional analysis of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis. The carotenoid biosynthesis gene cluster involved in astaxanthin production contained six carotenogenic genes (crtW, crtZ, crtY, crtI, crtB, and crtE genes) and recombinant E. coli harboring six carotenogenic genes from P. haeundaensis produced 400 ${\mu}g$/g dry cell weight (DCW) of astaxanthin. In order to increase production of astaxanthin in recombinant E. coli, we have cloned 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (lytB), farnesyl diphosphate (FPP) synthase (ispA), and isopentenyl (IPP) diphossphate isomerase (idi) in the isoprenoid pathway from E. coli and coexpressed these genes in recombinant E. coli harboring the astaxanthin biosynthesis genes. This engineered E. coli strain containing both isoprenoid pathway gene and astaxanthin biosynthesis gene cluster produced 1,200 ${\mu}g$/g DCW of astaxanthin, resulting 3-fold increased production of astaxanthin.
Keywords
Astaxanthin; carotenoid; isoprenoid; Paracoccus haeundaensis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Tarshis, L. C., M. Yan, C. D. Poulter and J. C. Sacchettini. 1994. Crystal structure of recombinant farnesyl diphosphate synthase at 2.6-Å resolution. Biochemistry 33, 10871-10877.   DOI   ScienceOn
2 Yoon, S. W., Y. M. Lee, J. E. Kim, S. H. Lee, J. H. Lee, J. Y. Kim, K. H. Jung, Y. C. Shin, J. D. Keasling and S. W. Kim. 2006. Enhanced lycopene production in Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate. Biotechnol. Bioeng. 94, 1025-1032.   DOI   ScienceOn
3 Yokoyama, A., H. Izumida and W. Miki. 1994. Production of astaxanthin and 4-ketozeaxanthin by the marine bacterium, Agrobacterium aurantiacum. Biosci. Biotechnol. Biochem. 58, 1842-1844.   DOI
4 Lee, P. C., B. N. Mijts and C. Schmidt-Dannert. 2004. Investigation of factors influencing production of the monocyclic carotenoid torulene in metabolically engineered Escherichia coli. Appl. Microbiol. Biotechnol. 65, 538-546.
5 Misawa, N., Y. Satomi, K. Kondo, A. Yokoyama, S. Kajiwara, T. Saito, T. Ohtani and W. Miki. 1995. Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177, 6575-6584.   DOI
6 Miller, M., W. M. Yoneyama and M. Soneda. 1976. Phaffia, a new yeast genus in the Deuteromycotina (Blastomycetes). Int. J. Syst. Bacteriol. 26, 286-291.   DOI
7 Neuman, I., H. Nahum and A. Ben-Amotz. 2000. Reduction of exercise-induced asthma oxidative stress by lycopene, a natural antioxidant. Allergy 55, 1184-1189.   DOI   ScienceOn
8 Murtaugh, M. A., K. N. Ma, J. Benson, K. Curtin, B. Caan and M. L. Slattery. 2004. Antioxidants, carotenoids and risk of rectal cancer. Am. J. Epidemiol. 159, 32-41.   DOI   ScienceOn
9 Starks, C. M., K. Back, J. Chappell and J. P. Noel. 1997. Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase. Science 277, 1815-1820.   DOI   ScienceOn
10 Rohmer, M. 1999. The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants. Nat. Prod. Rep. 16, 565-574.   DOI   ScienceOn
11 Tao, L., L. W. Wagner, P. E. Rouvière and Q. Cheng. 2006. Metabolic engineering for synthesis of aryl carotenoids in Rhodococcus. Appl. Microbiol. Biotechnol. 70, 222-228.   DOI   ScienceOn
12 Wendt, K. U., K. Poralla and G. E. Schulz. 1997. Structure and function of a squalene cyclase. Science 277, 1811-1815.   DOI   ScienceOn
13 Lee, J. H., Y. B. Seo, S. Y. Jeong, S. W. Nam and Y. T. Kim. 2007. Functional analysis of combinations in astaxanthin biosynthesis genes from Paracoccus haeundaensis. Biotecnol. Bioprocess Eng. 12, 312-317.   과학기술학회마을   DOI   ScienceOn
14 Tsubokura, A., H. Yoneda and H. Mizuta, 1999. Paracoccus carotinifaciens sp. nov., a new aerobic Gram‐negative astaxanthin‐ producing bacterium. Int. J. Syst. Bacteriol. 49, 277-282.   DOI   ScienceOn
15 Lee, J. H., Y. S. Kim, T. J. Choi, W. J. Lee and Y. T. Kim. 2004. Paracoccus haeundaensis sp. nov., a Gram-negative, halophilic, astaxanthin-producing bacterium. Int. J. Syst. Evol. Microbiol. 54, 1699-1702.   DOI   ScienceOn
16 Kurihara, H., H. Koda, S. Asami, Y. Kiso and T. Tanaka. 2002. Contribution of the antioxidative property of astaxanthin to its protective effect on the promotion of cancer metastasis in mice treated with restraint stress. Life Sci. 70, 2509-2520.   DOI   ScienceOn
17 Lee, J. H. and Y. T. Kim. 2006. Functional expression of the astaxanthin biosynthesis genes from a marine bacterium, Paracoccus haeundaensis. Biotechnol. Lett. 28, 1167-1173.   DOI   ScienceOn
18 Lee, J. H. and Y. T. Kim. 2006. Cloning and characterization of the astaxanthin biosynthesis gene cluster from the marine bacterium Paracoccus haeundaensis. Gene 370, 86-95.   DOI   ScienceOn
19 Lesburg, C. A., G, Zhai, D. E. Cane and D. W. Christianson. 1997. Crystal structure of pentalenene synthase: Mechanistic insights on terpenoid cyclization reactions in biology. Science 277, 1820-1824.   DOI   ScienceOn
20 Maateer, S., A. Coulson, N. Mclennan and M. Masters. 2001. The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid bioysynthesis. J. Bacteriol. 183, 7403-7407.   DOI   ScienceOn
21 Johnson, E. A. and G. H. An. 1991. Astaxanthin from microbial sources. Crit. Rev. Biotechnol. 11, 297-326.   DOI
22 Amar, E. C., V. Kiron, S. Satoh and T. Watanabe. 2004. Enhancement of innate immunity in rainbow trout (Oncorhynchus mykiss Walbaum) associated with dietary intake of carotenoids from natural products. Fish Shellfish Immunol. 16, 527-537.   DOI   ScienceOn
23 Alper, H., Y. S. Jin, J. F. Moxley and G. Stephanopoulos. 2005. Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli. Metab. Eng. 7, 155-164.   DOI   ScienceOn
24 Hix, L. M., S. F. Lockwood and J. S. Bertram. 2004. Bioactive carotenoids: potent antioxidants and regulators of gene expression. RedoxRep. 9, 181-191.
25 Johnson, E. A., D. B. Schuman and G. H. An. 1989. Isolation of mutants with increase astaxanthin content. Appl. Environ. Microbiol. 55, 116-124.
26 Giovannucci, E., A. Ascherio, E. B. Rimm, M. J. Stampfer, G. A. Colditz and W. C. Willett. 1995. Intake of carotenoids and retinol in relation to risk of prostate cancer. J. Natl. Cancer. Inst. 87, 1767-1776.   DOI
27 Harker, M., J. Hirschberg and A. Oren. 1998. Paracoccus marcusii sp. nov., an orange Gram‐negative coccus. Int. J. Syst. Bacteriol. 48, 543-548.   DOI   ScienceOn
28 Chew, B. P. and J. S. Park. 2004. Carotenoid action on the immune esponse. J. Nutr. 134, 257S-261S.   DOI
29 Fujisaki, S., H. Hara, Y. Nishimura, K. Horiuchi and T. Nishino. 1990. Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli. J. Biochem. 108, 995-1000.   DOI
30 Bubrick, P. 1991. Production of astaxanthin from Haematococcus. Bioresour. Technol. 38, 237-239.   DOI   ScienceOn
31 Wang, C. W., M. K. Oh and J. C. Liao. 1999. Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli. Biotechnol. Bioeng. 62, 235-241.   DOI   ScienceOn
32 Fujita, T., M. Satake, T. Watanabe, C. Kitajima, W. Miki, K. Yamaguchi and S. Konosu. 1983. Pigmentation of cultured red sea bream with astaxanthin diester purified from krill oil. Nippon Suisan Gakkaishi. 49, 1855-1865.   DOI
33 Ralley, L., E. M. Enfissi, N. Misawa, W. Schuch, P. M. Bramley and P. D. Fraser. 2004. Metabolic engineering of ketocarotenoid formation in higher plants. Plant J. 39, 477-486.   DOI   ScienceOn
34 Bertram, J. S. and A. L. Vine. 2005. Cancer prevention by retinoids and carotenoids: independent action on a common target. Biochim. Biophys. Acta. 1740, 170-178.   DOI   ScienceOn