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http://dx.doi.org/10.4014/jmb.1505.05035

Molecular Cloning and Co-Expression of Phytoene Synthase Gene from Kocuria gwangalliensis in Escherichia coli  

Seo, Yong Bae (Institute of Fisheries Science, College of Fisheries Science, Pukyong National University)
Choi, Seong-Seok (Department of Microbiology, Pukyong National University)
Lee, Jong Kyu (Department of Microbiology, Pukyong National University)
Kim, Nan-Hee (Department of Microbiology, Pukyong National University)
Choi, Mi Jin (Department of Marine Biomaterials and Aquaculture, Pukyong National University)
Kim, Jong-Myoung (Department of Marine Biomaterials and Aquaculture, Pukyong National University)
Jeong, Tae Hyug (Department of Marine and Fisheries Resources, Mokpo National University)
Nam, Soo-Wan (Department of Biotechnology and Bioengineering, Dong-Eui University)
Lim, Han Kyu (Department of Marine and Fisheries Resources, Mokpo National University)
Kim, Gun-Do (Department of Microbiology, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.11, 2015 , pp. 1801-1809 More about this Journal
Abstract
A phytoene synthase gene, crtB, was isolated from Kocuria gwangalliensis. The crtB with 1,092 bp full-length has a coding sequence of 948 bp and encodes a 316-amino-acids protein. The deduced amino acid sequence showed a 70.9% identity with a putative phytoene synthase from K. rhizophila. An expression plasmid, pCcrtB, containing the crtB gene was constructed, and E. coli cells containing this plasmid produced the recombinant protein of approximately 34kDa , corresponding to the molecular mass of phytoene synthase. Biosynthesis of lycopene was confirmed when the plasmid pCcrtB was co-transformed into E. coli containing pRScrtEI carrying the crtE and crtI genes encoding lycopene biosynthetic pathway enzymes. The results obtained from this study will provide a base of knowledge about the phytoene synthase of K. gwangalliensis and can be applied to the production of carotenoids in a non-carotenoidproducing host.
Keywords
Kocuria gwangalliensis; carotenoid; phytoene; lycopene; phytoene synthase;
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