The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
권호 표지
DOI QR코드

DOI QR Code

The Anti-inflammatory Effect of Cheongyeonsan for Allergic Rhinitis

알러지성 비염에 대한 청연산 추출물의 항염증효과

  • Kim, Jeong Won (School of Korean Medicine, Pusan National University) ;
  • Ahn, Sang Hyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Kim, Ki Bong (School of Korean Medicine, Pusan National University)
  • 김정원 (부산대학교 한의학전문대학원) ;
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 김기봉 (부산대학교 한의학전문대학원)
  • Received : 2018.01.16
  • Accepted : 2018.02.06
  • Published : 2018.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives This study investigated the effects of Cheongyeonsan for allergic rhinitis. Methods First, the GS/MS was used to analyze the effects of Cheongyeonsan by measuring inflammatory markers. Second, 3 groups of 10 6-week-old BALB/c mice were divided into Ctrl (no treatment), ARE (allergic rhinitis-induced without treatment), and CRT (allergic rhinitis-induced after Cheongyeonsan treatment) groups. Ovalbumin (OVA) was used as an antigen to induce allergic rhinitis and sensitization was performed by intraperitoneal injection of 0.1% OVA solution 21, 14, and 7 days before the onset of allergic rhinitis. Allergic rhinitis was induced by dropping OVA solution on the nasal cavity of each mouse for 5 days after the last sensitization. Seven days after the first induction, second induction was introduced by the same method. After making the section, MMP-9, substance P, $TNF-{\alpha}$, $NF-{\kappa}B$ p65, COX-2, iNOS and Nrf, apoptotic cells were observed by Masson trichrome staining, immunohistochemical staining, TUNEL of nasal mucosal tissues of each group. Results GC/MS results showed undecanoic acid. Masson trichrome results showed that the CRT group had less respiratory epithelial damage. Immunohistochemical staining showed CRT group had 58% decrease in MMP-9, 61% decrease in substance P, 55% decrease in $TNF-{\alpha}$, 38% decrease in $NF-{\kappa}B$ p65, 53% decrease in COX-2, 54% decrease in iNOS, 87% increase in Nrf compared to those of the ARE group. TUNEL showed a positive reaction of 84% increase in apoptotic cells greater than that of the ARE group. Conclusions Cheongyeonsan alleviates nasal mucosal damage and reduces inflammatory mediators from allergic rhinitis-induced mice.

Keywords

References

  1. Skoner DP. Allergic rhinitis: definition, epidemiology, pathophysiology, detection, and diagnosis. J Allergy Clin Immunol. 2001;108(1):S2-8. https://doi.org/10.1067/mai.2001.115569
  2. Lee JY, Kang KH, Kim MJ. The efficacy of alternative therapy of life quality improvement of allergic rhinits patients: by using ear acupuncture. J Kor Soc Esthe Cosm. 2011;6(2):107-22.
  3. Lee JG, Cho WJ, Hwang SY, Jee SY. The quality of life in patients with allergic rhinitis who visit Daegu oriental hospital of Daegu Haany university. J Korean Med Ophthalmol Otolaryngol Dermatol. 2007;20(3):191-203.
  4. Kim YE, Jeong UM, Lee DH. A survey on treatment trend for allergic rhinitis in Korean medicine clinic. J Korean Med Ophthalmol Otolaryngol Dermatol. 2017;30(4):75-96. https://doi.org/10.6114/JKOOD.2017.30.4.075
  5. Seidman MD, Gurgel RK, Lin SY, Schwartz SR, Baroody FM, Bonner JR, Dawson DE, Dykewicz MS, Hackell JM, Han JK, Ishman LS, Krouse HJ, Malekzadeh S, Mims JW, Omole FS, Reddy WD, Wallace DV, Walsh SA, Warren BE, Wilson MN, Nnacheta LC. Clinical practice guideline: allergic rhinitis. J Otolaryngol Head Neck Surg. 2015;152(1S):S1-43.
  6. Jeung CW, Jo HG, Kim HH, Song MY. Current update on allergic rhinitis for Korean medicine management. J Korean Med Ophthalmol Otolaryngol Dermatol. 2016;29(4):95-113. https://doi.org/10.6114/jkood.2016.29.4.095
  7. Curhan SG, Shargorodsky J, Eavey R, Curhan GC. Analgesic use and the risk of hearing loss in women. Am J Epidemiol. 2012;176(6):544-54. https://doi.org/10.1093/aje/kws146
  8. Curhan SG, Eavey R, Shargorodsky J, Curhan GC. Analgesic use and the risk of hearing loss in men. Am J Med. 2010;123(3):231-7. https://doi.org/10.1016/j.amjmed.2009.08.006
  9. Park SE, Hong SU. The treatment effect of Younggamgangmisinhayin-tang through control of Th 2 cell differentiation in allergic rhinitis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2008;21(1):83-95.
  10. Park JH, Hong SU. The effects of Hyunggaeyungyo-tang of suppression of iNOS production on mice with allergic rhinitis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2012;25(1):12-21. https://doi.org/10.6114/jkood.2012.25.1.012
  11. Kim DH, Hong SU. The effect of Bangpungtongsungsan on allergic rhinitis of obese mouse fed high fat diet. J Korean Med Ophthalmol Otolaryngol Dermatol. 2016;29(1):1-15. https://doi.org/10.6114/jkood.2016.29.1.001
  12. Jeung CW, Jo HG, Kim HH, Song MY. Current update on allergic rhinitis for Korean medicine management. J Korean Med Ophthalmol Otolaryngol Dermatol. 2016;29(4):95-113. https://doi.org/10.6114/jkood.2016.29.4.095
  13. Jang BH, Choi IH, Kim KS, Kim HT, Kim KJ, Kim MH, Park JS, Ko SG. Current status of allergic rhinitis patients in Korean medicine hospitals - exploratory study based on electronic medical records of 3 hospitals. J Korean Med Ophthalmol Otolaryngol Dermatol. 2014;27(1):117-29. https://doi.org/10.6114/jkood.2014.27.1.117
  14. Kim NK, Lee DH, Seo ES, Choi EJ, Jang BH, Ko SG. Treatment packages of persistent allergic rhinitis for developing PRCT protocols: an expert survey. Kor J Orient Prev Med Soc. 2013;17(3):143-53.
  15. Ozaki Y, Rui J, Tang YT. Antiinflammatory effect of Forsythia Suspensa VAHL and its active principle. Biol Pharm Bull. 2000;23(3):365-7. https://doi.org/10.1248/bpb.23.365
  16. Hwang MK, Choi YJ, Kim MJ, Lee B, Jung HS, Sohn YJ. The inhibitory effects of GHJ on allergic inflammatory response in human mast cells (HMC-1). J Herb Formula Sci. 2017;25(2):167-77. https://doi.org/10.14374/HFS.2017.25.2.167
  17. Lee JS, Hong YH. An immunohistochemical study for the T and B lymphocyte on the mucosa of allergic rhinitis. Chung-Ang J Med. 1993;19(3):351-60.
  18. Amin K. The role of mast cells in allergic inflammation. Respir Med. 2012;106(1):9-14. https://doi.org/10.1016/j.rmed.2011.09.007
  19. Yong VW, Power C, Forsyth P, Edwards DR. Metalloproteinases in biology and pathology of the nervous system. Nat Rev Neurosci. 2001;2(7):502-11. https://doi.org/10.1038/35081571
  20. Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev. 1997;77(4):1033-79. https://doi.org/10.1152/physrev.1997.77.4.1033
  21. Kim JH, Kim EJ. Effect of anti-oxidant, anti-inflammatory and anti-invasive of PMA-induced matrix metalloproteinase (MMP-2) and MMP-9 activities of water extract and solvent fractions of Saururus Chinensis. J Life Sci. 2016;26(5):584-91. https://doi.org/10.5352/JLS.2016.26.5.584
  22. Burrage PS, Mix KS, Brinckerhoff CE. Matrix metalloproteinases: role in arthritis. Front Biosci. 2006;11:529-43. https://doi.org/10.2741/1817
  23. Yong VW, Power C, Forsyth P, Edwards DR. Metalloproteinases in biology and pathology of the nervous system. Nat Rev Neurosci. 2001;2(7):502-11. https://doi.org/10.1038/35081571
  24. Power C, Henry S, Del Bigio MR, Larsen PH, Corbett D, Imai Y, Yong VW, Peeling J. Intracerebral hemorrhage induces macrophage activation and matrix metalloproteinases. Ann Neurol. 2003;53(6):731-42. https://doi.org/10.1002/ana.10553
  25. Thu NB, Trung TN, Ha DT, Khoi NM, Than NV, Soulinho T, Nam NH, Phuong TT, Bae KH. Articles: Zanthoxylum rhetsa stem bark extract inhibits LPS-induced COX-2 and iNOS expression in RAW 264.7 cells via the NF-kB inactivation. Nat Prod Sci. 2010;16(4): 265-70.
  26. Shin SM, Yang JH, Ki SH. Role of the Nrf2-ARE pathway in liver diseases. Oxid Med Cell Longev. 2013;2013:763257.
  27. Kim NH, Park HJ, Kim IS. Auranofin downregulates nuclear $factor-{\kappa}B$ activation via Nrf2-independent mechanism. J Life Sci. 2010;20(12):1772-6. https://doi.org/10.5352/JLS.2010.20.12.1772
  28. Dong Z, Saikumar P, Weinberg JM, Venkatachalam MA. Calcium in cell injury and death. Annu Rev Pathol. 2006;1:405-34. https://doi.org/10.1146/annurev.pathol.1.110304.100218