• Asian-Australasian Journal of Animal Sciences
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• v.9 no.5
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• pp.509-513
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• 1996

The data analysed consisted of body weight records at birth, and at 3, 6, 9 and 12 months of age of 549 half Friesian $\times$ Bunaji crossbred heifers collected over a twenty-five year period (1965-1989). Least squares $means{\pm}s.e$. of body weights at birth, 3, 6, 9 and 12 months of age were $26.7{\pm}1.3$, $72.4{\pm}4.5$, $112.9{\pm}6.9$, $147.2{\pm}9.2$ and $182.1{\pm}11.1kg$, respectively. Year of birth was highly significant (p < 0.01) in affecting body weights at all ages, while the effect of month of birth was not. Seasonal influence on birth weight and body weights at 3 and 6 months of age was significant (p < 0.05). Phenotypic correlations between body weights at all ages were positive and highly significant (p < 0.01), ranging from 0.30 to 0.79. The results of this study showed that the beneficial effect of crossbreeding Friesian with Bunaji cattle was reflected in the growth performance of the $F_1$ crosses, since they grew faster than the indigenous Bunaji from brith to yearling age. The study also indicated that heifer selection for yearling body weight can be done early on the basis of weights at 3 and 6 months of age.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.5
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• pp.612-619
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• 2015

Current study aimed to evaluate the performance of bulls (1/2 Purun$\tilde{a}$ vs 1/2 Canchim) slaughtered at two ages and three different weights. One hundred and thirteen bulls were divided into two slaughter ages (16 and 22 months) and three different slaughter weights (light, 422 kg; medium, 470 kg; and heavy, 550 kg). The body weight was higher for bulls slaughtered at 16 months. Daily gain, carcass weight and dressing were higher for bulls slaughtered at 16 months. Feed intake was higher for bulls slaughtered at 22 months although feed efficiency was better for bulls slaughtered at 16 months. Carcass characteristics were better for bulls slaughtered at 16 months. The percentages of muscle, fat and bone and meat characteristics were similar between two slaughter ages. Feed intake and animal performance was lower for lighter animals. Feed conversion and carcass dressing were similar in the three slaughter weights. Muscle percentage was higher for heavier animals but fat and bone percentages were lower. Slaughter weight had no effect on meat characteristics.

• Korean Journal of Poultry Science
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• v.48 no.2
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• pp.59-67
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• 2021

This study investigated the morphological features, growth, and meat yield performance of Pekin (P), Nageswari (N), and their reciprocal F₁ crossbreds (P♂×N♀ and N♂×P♀). A total of 301-day-old ducklings were reared in four different pens up to 20 weeks of age under intensive management conditions. Feeding and management practices were similar for all individuals throughout the experimental period. The morphology and plumage pattern of F₁ crossbreds were similar to those of indigenous Nageswari ducks because of the dominant inheritance of the extended Black allele (E locus). Genotype had significant differences (P<0.05) among the four genotypes in morphometric measurements, except wing and shank length. Growth performance was highly significant among the four genotypes (P<0.001) from one-day to 12 weeks of age. The average live weights of P, N, P♂×N♀ and N♂×P♀ crossbred genotypes at 12 weeks of age were 2038.35±29.74, 1542.44±33.61, 1851.85±28.59 and 1691.08±27.80 g, respectively. Meat yield parameters varied significantly (P<0.05) among the different genotypes for all studied traits, except for liver and gizzard weight. Moreover, no significant differences (P>0.05) were observed between P and P♂×N♀ crossbred for important meat yield traits such as hot carcass weight, dressing%, back half weight, drumstick with thigh weight and breast meat weight. Remarkably, the P♂×N♀ crossbreed possesses 50% native inheritance, which contributes to better adaptation in a hot-humid environment. Our results revealed that the P♂×N♀ genotype could be suitable for higher meat production with better adaptability in the agro-climatic conditions of Bangladesh.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.10
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• pp.1491-1500
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• 2019

Objective: European pigs have been imported to improve the economically important traits of Thai pigs by crossbreeding and was finally completely replaced. Currently Thai indigenous pigs are particularly kept in a small population. Therefore, indigenous pigs risk losing their genetic diversity and identity. Thus, this study was conducted to perform large-scale genetic diversity and phylogenetic analyses on the many pig breeds available in Thailand. Methods: Genetic diversity and phylogenetics analyses of 222 pigs belonging to Thai native pigs (TNP), Thai wild boars (TWB), European commercial pigs, commercial crossbred pigs, and Chinese indigenous pigs were investigated by genotyping using 26 microsatellite markers. Results: The results showed that Thai pig populations had a high genetic diversity with mean total and effective ($N_e$) number of alleles of 14.59 and 3.71, respectively, and expected heterozygosity ($H_e$) across loci (0.710). The polymorphic information content per locus ranged between 0.651 and 0.914 leading to an average value above all loci of 0.789, and private alleles were found in six populations. The higher $H_e$ compared to observed heterozygosity ($H_o$) in TNP, TWB, and the commercial pigs indicated some inbreeding within a population. The Nei's genetic distance, mean $F_{ST}$ estimates, neighbour-joining tree of populations and individual, as well as multidimensional analysis indicated close genetic relationship between Thai indigenous pigs and some Chinese pigs, and they are distinctly different from European pigs. Conclusion: Our study reveals a close genetic relationship between TNP and Chinese pigs. The genetic introgression from European breeds is found in some TNP populations, and signs of genetic erosion are shown. Private alleles found in this study should be taken into consideration for the breeding program. The genetic information from this study will be a benefit for both conservation and utilization of Thai pig genetic resources.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8
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• pp.1069-1076
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• 2019

Objective: Yunnan is not only a frontier zone that connects China with South and Southeast Asia, but also represents an admixture zone between taurine (Bos taurus) and zebu (Bos indicus) cattle. The purpose of this study is to understand the level of genomic diversity and the extent of admixture in each Yunnan native cattle breed. Methods: All 120 individuals were genotyped using Illumina BovineHD BeadChip (777,962 single nucleotide polymorphisms [SNPs]). Quality control and genomic diversity indexes were calculated using PLINK software. The principal component analysis (PCA) was assessed using SMARTPCA program implemented in EIGENSOFT software. The ADMIXTURE software was used to reveal admixture patterns among breeds. Results: A total of 604,630 SNPs was obtained after quality control procedures. Among six breeds, the highest level of mean heterozygosity was found in Zhaotong cattle from Northeastern Yunnan, whereas the lowest level of heterozygosity was detected in Dehong humped cattle from Western Yunnan. The PCA based on a pruned dataset of 233,788 SNPs clearly separated Dehong humped cattle (supposed to be a pure zebu breed) from other five breeds. The admixture analysis further revealed two clusters (K = 2 with the lowest cross validation error), corresponding to taurine and zebu cattle lineages. All six breeds except for Dehong humped cattle showed different degrees of admixture between taurine and zebu cattle. As expected, Dehong humped cattle showed no signature of taurine cattle influence. Conclusion: Overall, considerable genomic diversity was found in six Yunnan native cattle breeds except for Dehong humped cattle from Western Yunnan. Dehong humped cattle is a pure zebu breed, while other five breeds had admixed origins with different extents of admixture between taurine and zebu cattle. Such admixture by crossbreeding between zebu and taurine cattle facilitated the spread of zebu cattle from tropical and subtropical regions to other highland regions in Yunnan.

• Animal Bioscience
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• v.34 no.4
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• pp.751-758
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• 2021

Objective: The objectives of this study were to investigate the relationship between the mRNA expression of adipocyte type fatty acid binding protein (A-FABP) and heart type FABP (H-FABP) in Thai native chicken crossbreeds and evaluate the level of exotic inclusion in native chicken that will improve growth while maintaining its relatively low carcass fat. Methods: The fat deposition traits and mRNA expression of A-FABP and H-FABP were evaluated at 6, 8, 10, and 12 weeks of age in 4 chicken breeds (n = 8/breed/wk) (100% Chee breed [CH] [100% Thai native chicken background], CH male and broiler female [Kaimook e-san1; KM1] [50% CH background], broiler male and KM1 female [Kaimook e-san2; KM2] [25% CH background], and broiler [BR]) using abdominal fat (ABF) and muscular tissues. Results: The BR breed was only evaluated at 6 weeks of age. At week 6, the CH breed had a significantly lower A-FABP expression in ABF and intramuscular fat (IF) compared with the other breeds. At 8 to 12 weeks, the KM2 groups showed significant upregulation (p<0.05) of A-FABP in both ABF and IF compared to the CH and KM1 groups. The expression of H-FABP did not follow any consistent pattern in both ABF and IF across the different ages. Conclusion: Some level of crossbreeding CH chickens can be done to improve growth rate while maintaining their low ABF and IF. The expression level of A-FABP correlate with most fat traits. There was no consistency of H-FABP expression across breed. A-FABPs is involved in fat deposition, genetic markers in these genes could be used in marker assisted studies to select against excessive fat accumulation.

• Korean Journal of Plant Taxonomy
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• v.52 no.4
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• pp.226-234
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• 2022

The study aims are to examine the characteristics of artificial and natural hybrids between Viola albida var. albida (= albida, from below) and V. albida var. chaerophylloides (= chaerophylloides, from below), and to confirm if hybrids could be fertile and make populations in their native habitats. The 1st filial (= F₁, from below) leaf shape produced by artificial crossing between albida and chaerophylloides was the same as that of V. albida var. takahashii (= takahashii, from below), and F₁ bore also both chasmogamous and cleistogamous flowers. F₁ seed number was 9.6 per cleistogamous pods, which was remarkably less than the average of 38.2 for albida and chaerophylloides, but the germination rate was all similar. The leaf type of self-crossed 2nd offsprings (= F₂, from below) showed all leaf types found in the Viola albida complex, but the ratio of chaerophylloides leaf type was relatively low. Individuals whose F₂ leaf type was restored to albida produced an average of 31.4 seeds per capsule, meaning that fertility was restored. On the other hand, individuals of F₂ takahashii leaf type come to fruition a low average of 10.4 seeds per capsule, which is similar to that of takahashii. The results of crossbreeding experiment, where is their native habitats, were similar to that of laboratory. Both albida and chaerophylloids in Mt. Bulmyeong distribute extensively, but takahashii make a small population only in places where albida and chaerophylloides grow together. Summarizing the above results is suggesting that the speciation of takahashii was done by hybrid between albida and chaerophylloides, and these have been maintained with relatively small population by cleistogamous capsules.

• Journal of Animal Science and Technology
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• v.65 no.1
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• pp.160-174
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• 2023

The purpose of this study was to compare marbling score, meat quality, juiciness, sarcomere length, and skeletal muscle satellite cell (SMSC) growth and related gene expression between Woori black pig (WB) and the Landrace, Yorkshire, and Duroc (LYD) crossbreed at different body weights (b.w.). WB was developed to improve meat quality and growth efficiency by crossbreeding Duroc with Korean native black pig. A total of 24 pigs were sacrificed when their b.w. reached about 50, 75, 100, and 120 kg. SMSC were isolated from the femoris muscles, and muscle and adipose tissues were sampled from the middle and the subcutaneous part of the femoris of hind legs, respectively. Expression levels of genes including Myoblast determination protein 1 (MyoD), Paired box gene 3 (Pax3), Myosin heavy chain (MyHC), and Myogenin, which are responsible for the growth and development of SMSC, were higher in LYD than the WB. Muscle growth inhibitor myostatin (MSTN), however, was expressed more in WB compared to LYD (p < 0.01). Numbers of SMSC extracted from femoris muscle of LYD at 50, 75, 100, and 120 kg b.w. were 8.5 ± 0.223, 8.6 ± 0.245, 7.2 ± 0.249, and 10.9 ± 0.795, and those from WB were 6.2 ± 0.32, 6.2 ± 0.374, 5.3 ± 0.423, and 17.1 ± 0.315, respectively. Expression of adipogenic genes in adipose tissue including CCAAT/enhancer-binding protein (CEBP)-β, peroxisome proliferator activated receptor (PPAR)-γ, and fatty acid synthase (FASN), were greater in WB when compared with LYD (p < 0.01). Results from the current study suggest that different muscle cell numbers between 2 different breeds might be affected by related gene expression and this warrants further investigation on other growth factors regulating animal growth and development.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.382-390
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• 2015

This work was conducted to investigate the performance and meat characteristics of commercial Korean native duck (KND). A total of 180 1-d-old ducklings of 2-way crossbreds from A and B lines (from National Institute of Animal Science) were used in this work and divided into 4 groups (3 replicates/group, 15 birds/replicate). The four groups were 4 crossbreds as AA (A line [♀]${\times}$A line [♂]), AB (A line [♀]${\times}$B line [♂]), BB (Pure line B strains) and BA (B strains [♀]${\times}$A strain [♂]). Ducks were fed diets based on corn-soybean meal for 0 to 3 wk (22.4% crude protein [CP], 2,945 kcal/kg metabolizable energy [ME]) and 3 to 8 wk (18.4% CP, 3,047 kcal/kg ME). As a result of this study, average body weight of 4 crossbreds were 625, 1,617, 2,466, and 2,836 g at 2, 4, 6, and 8 weeks, respectively, and significantly increased over the period of time (p<0.05). Body weight of BB group was greater than other crossbreds at the age of 6 weeks (p<0.05). There was a significant difference in weekly body weight gains (p<0.05), which were 573, 991, 850, and 371 g at 2, 4, 6, and 8 weeks old, respectively. Uniformity of 4 crossbreds was 84.9%, 80.5%, and 72.5% at 6, 7, and 8 weeks, respectively, and there was no difference among crossbreds. Body weight gain of BB crossbred was highest among crossbreds (p<0.05). Weekly feed intake significantly increased with weeks as 669, 1,839, 2,812, and 3,381 g at 2, 4, 6, and 8 weeks respectively (p<0.05). Feed intakes of AA and BB crossbreds were higher at 2 to 4 weeks old than others and that of BB crossbred was highest at 4 to 6 weeks old (p<0.05). Weekly feed conversion ratios were 1.17, 1.86, 3.32, and 9.37 at 0 to 2, 2 to 4, 4 to 6, and 6 to 8 weeks old, respectively, and it increased with age (p<0.05). There was no significant difference in feed conversion ratio among crossbreds. Carcass yields of 4 crossbreds were 73.6%, 71.6%, 73.5%, and 71.7%, respectively, so there was no significant difference among crossbreds. There was no difference in wing, neck, breast and leg ratios among crossbreds. However, back ratios of 4 crossbreds were 17.6%, 18.0%, 15.8%, and 17.6%, respectively, and back ratio of BB was the highest among crossbreds. Finally, these results may provide the basic data on the production, carcass quality, fatty acid and amino acid composition of commercial KND with 2-way crossbreeding.

• Korean Journal of Poultry Science
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• v.48 no.3
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• pp.101-110
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• 2021

In this study, we performed a cross-combination test using offspring from two paternal and five maternal parent stock lines to produce a new synthetic breed of Korean native commercial chickens. The survival rate, body weight, feed intake, and feed efficiency were measured for 10 crosses, with 837 Hanhyup-3 chickens used as a control. Most chickens had brown-based feathers, and there was little difference in color phenotypes among combinations. The average survival rate of the combinations was 94.9%, which was significantly different from that of Hanhyup-3 at 75.3% (P<0.01). There was significant differences in body weight among the combinations at all weeks of age (P<0.01). At 10 weeks of age, the FHHY combination was the heaviest with 2,300 g, while the weight of Hanhyup-3 was 1,922 g. In addition, the weight difference between the male and female chickens was 458 g. As a result of regression analysis, the duration needed to reach 1.9 kg was about 61 days for the FHFY, FHHY, and HFFY combinations, and 70 days for Hanhyup-3, exhibiting a 9-day difference. The average feed conversion ratio of the combinations until 10 weeks was 2.42, which was significantly different from that of Hanhyup-3 at 2.97 (P<0.01). In conclusion, FHHY, FHFY, and HFFY were determined to be the best new breed combinations based on production capacity. When these combinations are used as new commercial breeds, they are expected to increase the average weight by 18% and improve feed efficiency by 19%, as compared to the existing Hanhyup-3 breed.