Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Gene expression of fatty acid binding protein genes and its relationship with fat deposition of Thai native crossbreed chickens

  • Tunim, Supanon (Department of Animal Science, Faculty of Agriculture, Khon Kaen University) ;
  • Phasuk, Yupin (Department of Animal Science, Faculty of Agriculture, Khon Kaen University) ;
  • Aggrey, Samuel E. (NutriGenomics Laboratory, Department of Poultry Science, University of Georgia) ;
  • Duangjinda, Monchai (Department of Animal Science, Faculty of Agriculture, Khon Kaen University)
  • Received : 2020.01.14
  • Accepted : 2020.04.06
  • Published : 2021.04.01
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Abstract

Objective: The objectives of this study were to investigate the relationship between the mRNA expression of adipocyte type fatty acid binding protein (A-FABP) and heart type FABP (H-FABP) in Thai native chicken crossbreeds and evaluate the level of exotic inclusion in native chicken that will improve growth while maintaining its relatively low carcass fat. Methods: The fat deposition traits and mRNA expression of A-FABP and H-FABP were evaluated at 6, 8, 10, and 12 weeks of age in 4 chicken breeds (n = 8/breed/wk) (100% Chee breed [CH] [100% Thai native chicken background], CH male and broiler female [Kaimook e-san1; KM1] [50% CH background], broiler male and KM1 female [Kaimook e-san2; KM2] [25% CH background], and broiler [BR]) using abdominal fat (ABF) and muscular tissues. Results: The BR breed was only evaluated at 6 weeks of age. At week 6, the CH breed had a significantly lower A-FABP expression in ABF and intramuscular fat (IF) compared with the other breeds. At 8 to 12 weeks, the KM2 groups showed significant upregulation (p<0.05) of A-FABP in both ABF and IF compared to the CH and KM1 groups. The expression of H-FABP did not follow any consistent pattern in both ABF and IF across the different ages. Conclusion: Some level of crossbreeding CH chickens can be done to improve growth rate while maintaining their low ABF and IF. The expression level of A-FABP correlate with most fat traits. There was no consistency of H-FABP expression across breed. A-FABPs is involved in fat deposition, genetic markers in these genes could be used in marker assisted studies to select against excessive fat accumulation.

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References

  1. World Health Organization. World health statistics 2018: monitoring health for the SDGs, sustainable development goals. World Health Organization; 2018.
  2. International Diabetes Federation. Global guideline for type 2 diabetes. Brussel, Belgium: International Diabetes Federation; 2005.
  3. Ahmad Nizar NN, Nazrim Marikkar JM, Hashim DM. Differentiation of lard, chicken fat, beef fat and mutton fat by GCMS and EA-IRMS techniques. J Oleo Sci 2013;62:459-64. https://doi.org/10.5650/jos.62.459
  4. Jaturasitha S, Srikanchai T, Kreuzer M, Wicke M. Differences in carcass and meat characteristics between chicken indigenous to northern Thailand (Black-boned and Thai native) and imported extensive breeds (Bresse and Rhode Island Red). Poult Sci 2008;87:160-9. https://doi.org/10.3382/ps.2006-00398
  5. Zerehdaran S, Vereijken ALJ, van Arendonk JAM, van der Waaijt EH. Estimation of genetic parameters for fat deposition and carcass traits in broilers. Poult Sci 2004;83:521-5. https://doi.org/10.1093/ps/83.4.521
  6. Wang Q, Guan T, Li H, Bernlohr DA. A novel polymorphism in the chicken adipocyte fatty acid-binding protein gene (FABP4) that alters ligand-binding and correlates with fatness. Comp Biochem Physiol B Biochem Mol Biol 2009;154:298-302. https://doi.org/10.1016/j.cbpb.2009.07.002
  7. Liu ZW, Fan HL, Liu XF, et al. Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice. Genet Mol Res 2015;14:2742-9. https://doi.org/10.4238/2015.March.31.4
  8. Wang Y, Shu D, Li L, Qu H, Yang C, Zhu Q. Identification of single nucleotide polymorphism of H-FABP gene and its association with fatness traits in chickens. Asian-Australas J Anim Sci 2007;20:1812-9. https://doi.org/10.5713/ajas.2007.1812
  9. Tyra M, Zak G. Analysis of relationships between fattening and slaughter performance of pigs and the level of intramuscular fat (IMF) in longissimus dorsi muscle. Ann Anim Sci 2012;12:169-78. https://doi.org/10.2478/v10220-012-0014-6
  10. van der Horst DJ, van Doorn JM, Passier PCCM, Vork MM, Glatz JFC. Role of fatty acid-binding protein in lipid metabolism of insect flight muscle. Mol Cell Biochem 1993;123:145-52. https://doi.org/10.1007/BF01076486
  11. Furuhashi M, Hotamisligil GS. Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nat Rev Drug Discov 2008;7:489-503. https://doi.org/10.1038/nrd2589
  12. Shi H, Wang Q, Wang Y, et al. Adipocyte fatty acid-binding protein: an important gene related to lipid metabolism in chicken adipocytes. Comp Biochem Physiol B Biochem Mol Biol 2010;157:357-63. https://doi.org/10.1016/j.cbpb.2010.08.005
  13. Li DL, Chen JL, Wen J, Zhao GP, Zheng MQ, Liu C. Growth, carcase and meat traits and gene expression in chickens divergently selected for intramuscular fat content. Br Poult Sci 2013;54:183-9. https://doi.org/10.1080/00071668.2013.771392
  14. Teltathum T, Mekchay S. Proteome changes in Thai indigenous chicken muscle during growth period. Int J Biol Sci 2009;5:679-85. https://doi.org/10.7150/ijbs.5.679
  15. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  16. Havenstein GB, Ferket PR, Grimes JL, Qureshi MA, Nestor KE. Comparison of the performance of 1966- versus 2003-type Turkeys when fed representative 1966 and 2003 Turkey diets: growth rate, livability, and feed conversion. Poult Sci 2007;86:232-40. https://doi.org/10.1093/ps/86.2.232
  17. Chambers JR. Genetics of growth and meat production in chickens. In: Crawford RD, editor. Poultry breeding and genetics. Amsterdam, Netherlands: Elsevier; 1990. pp. 599-643.
  18. Yin HD, Gilbert ER, Chen SY, et al. Effect of hybridization on carcass traits and meat quality of Erlang mountainous chickens. Asian-Australas J Anim Sci 2013;26:1504-10. https://doi.org/10.5713/ajas.2013.13097
  19. Promket D, Ruangwittayanusorn K, Somchan T. The study of carcass yields and meat quality in crossbred native chicken (Chee). Agric Agric Sci Procedia 2016;11:84-9. https://doi.org/10.1016/j.aaspro.2016.12.014
  20. Hidayat C, Iskandar S, Sartika T, Wardhani T. Growth response of improved native breeds of chicken to diets differed in energy and protein content. J Ilmu Ternak Dan Vet 2016;21:174-81. https://doi.org/10.14334/jitv.v21i3.1397
  21. Michal JJ, Zhang ZW, Gaskins CT, Jiang Z. The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu × Limousin F2 crosses. Anim Genet 2006;37:400-2. https://doi.org/10.1111/j.1365-2052.2006.01464.x
  22. Chmurzynska A, Mackowski M, Szydlowski M, et al. Polymorphism of intronic microsatellites in the A-FABP and LEPR genes and its association with productive traits in the pig. J Anim Feed Sci 2004;13:615-24.
  23. Li WJ, Li HB, Chen JL, Zhao GP, Zheng MQ, Wen J. Gene expression of heart- and adipocyte-fatty acid-binding protein and correlation with intramuscular fat in Chinese chickens. Anim Biotechnol 2008;19:190-4. https://doi.org/10.1080/10495390802058319
  24. Wang Y, He J, Yang W, et al. Correlation between heart-type fatty acid-binding protein gene polymorphism and mRNA expression with intramuscular fat in Baicheng-oil chicken. Asian-Australas J Anim Sci 2015;28:1380-7. https://doi.org/10.5713/ajas.14.0886
  25. Chen QM, Wang H, Zeng YQ, Chen W. Developmental changes and effect on intramuscular fat content of H-FABP and A-FABP mRNA expression in pigs. J Appl Genet 2013;54:119-23. https://doi.org/10.1007/s13353-012-0122-0
  26. Ye MH, Chen JL, Zhao GP, Zheng MQ, Wen J. Associations of A-FABP and H-FABP markers with the content of intramuscular fat in Beijing-You chicken. Anim Biotechnol 2009;21:14-24. https://doi.org/10.1080/10495390903328116
  27. Guo X, Tang R, Wang W, Liu D, Wang K. Effects of dietary protein/carbohydrate ratio on fat deposition and gene expression of peroxisome proliferator activated receptor γ and heart fatty acid-binding protein of finishing pigs. Livest Sci 2011;140:111-6. https://doi.org/10.1016/j.livsci.2011.02.016
  28. Zhang YJ, Liu YQ, Song J, Cheng SY, Dong LX. Effects of dietary energy level on the transcription of the H-FABP gene in different tissues of sheep. Small Rumin Res 2013;115:29-33. https://doi.org/10.1016/j.smallrumres.2013.09.007
  29. Shang Z, Guo L, Wang N, Shi H, Wang Y, Li H. Oleate promotes differentiation of chicken primary preadipocytes in vitro. Biosci Rep 2014;34:e00093. https://doi.org/10.1042/BSR20130120
  30. Fenwick MA, Fitzpatrick R, Kenny DA, et al. Interrelationships between negative energy balance (NEB) and IGF regulation in liver of lactating dairy cows. Domest Anim Endocrinol 2008;34:31-44. https://doi.org/10.1016/j.domaniend.2006.10.002

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