• Title/Summary/Keyword: cross alignment

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Genotypic Variations among Human Caliciviruses in Korea: 1987-1994 (한국에 산재하는 사람 Caliciviruses의 다양한 유전자군: 1987-1994년)

  • Nam, Ki-Bum;Kim, Ji-Aee;Yang, Jai-Myung;Kim, Kyung-Hee
    • The Journal of Korean Society of Virology
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    • v.27 no.2
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    • pp.185-195
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    • 1997
  • Sequence comparison of the RNA-dependent RNA polymerase of human caliciviruses (HuCVs) from Korean children with gastroenteritis revealed significant genetic variation among them. cDNA clones were produced from the HuCVs collected from pediatric population during a period of 1987-1994. The application of reverse transcription-polymerase chain reaction (RT-PCR) using primers directed to the RNA-dependent RNA polymerase region within ORF1 of Norwalk virus (NV) showed that 13.7% of HuCVs yielded PCR products of similar size to the NV prototype, NV8FIIa/68/US, with exceptions of HuCV 185/87/Korea and HuCV 1115/90/Korea. Computer analyses showed that the PCR products had a continuous protein encoding frame on the positive strand, and contained GLPSG and YGDD amino acid motifs at the predicted distance from primers. Alignment of the amino acid sequences of HuCVs with previously published sequences for Snow Mountain agent (SMA), NV, and Sapporo/82/Japan indicated that these strains can be divided into four major genogroups. There were 10 (45%) SMA-like CVs, one (4.5%) NV-like HuCVs, two (9%) Sapporo-like HuCVs, and nine (41%) unidentified HuCVs. This fourth genogroup should be investigated further. HuCV 185/87/Korea and HuCV 1115/90/Korea, Sapporo-like CVs, were genetically distinct from previously characterized HuCVs and more closely related to known animal CVs. One of the animal CV-like strain, HuCV 185/87/Korea, showed nucleotide and amino acid homology of only 67% and 73% with the prototype Sapporo/82/Japan. Further characterization of animal and human CV genomes and studies of possible cross-transmission of CVs from animals to humans are likely to be beneficial in understanding the epidemiology of HuCVs.

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Characteristics of Symmetric-Shape Parts Shearing on Micro NCT (마이크로 NCT에 의한 대칭형상구멍의 전단특성)

  • Hong N. P.;Kim B. H.;Chang I. B.;Kim H. Y.;Oh S. I.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2002.02a
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    • pp.285-291
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    • 2002
  • The shearing process for the sheet metal is normally used in the precision elements such as a frame of TFT-LCD or lead frame of If chips. In these precision elements, the burr formation prevents the system assembly and needs the additional burr removing process. In this paper, we developed the small size NC punching system which has an aligning kinematics between the rectangular shaped punch and die. The punch is driven by an ai cylinder and the sheet metal is moving on the X-Y table system which is driven by two stewing motors. The microprocessor control the whole system and communicate with the monitoring PC by RS232C serial communication protocol. The graphic user interface program in PC monitors nil control the punching system. The cross shaped joint hinge supports the punching die and positioned by two differential screws, whose are installed in perpendicular directions. The aligning between the punch and die is performed using the sheets of half thickness(0.1mm Brass) of the real process for the frame of the TFT-LCD. Using half thickness Brass, the burr formation is magnified and we can decide the aligning direction more easily then using the real thickness(0.2mm) Aluminum. In this paper, the aligning results are measured manually using the SEM photographs and we hope to make the automated aligning procedures using some kinds of image processing techniques.

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Highway Maintenance Cost Optimization Using GSIS (지형정보를 이용한 도로의 최적 유지관리 비용 산정)

  • 강인준;이준석
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.20 no.4
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    • pp.367-374
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    • 2002
  • Maintainability of highways is an important problem that is considered in the planning steps of a highway development process. A number of asset management systems have been developed to precisely predict maintenance and pavement expenditures for better decision making, But these systems are not helpful in reducing maintenance costs. Optimization of some highway design characteristics in the planning phases may reduce maintenance costs over the life cycle of highway. The formulations for initial and maintenance costs have been developed based on which design variables can be chosen to minimize these costs, focusing on the sideslope in cut and fill sections. Maintenance cost has been represented as a function of sideslope, width of highway cross section, and annual average daily traffic. A real geographic database of between Chung joo and Sang joo city in Choong buk was used and it is presented to investigate the sensitivities of maintenance cost and soil characteristics in selecting alignments. In this study, we present that maintenance cost and soil characteristics are important considerations in alignment optimization.

RETRIEVAL OF LOCAL INTERPLANETARY DUST EMISSIVITY BY ASTRO-F

  • HONG S. S.;KWON S. M.;PYO J.;UENO M.;ISHIGURO M.;USUI F.;WEINBERG J. L.
    • Journal of The Korean Astronomical Society
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    • v.37 no.4
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    • pp.159-169
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    • 2004
  • This is a proposal to probe local part of the interplanetary dust (IPD) cloud complex and retrieve mean volume emissivity of the local IPDs at mid-infrared wavelengths. This will be done by monitoring, with Infrared Camera (IRC) aboard the ASTRO-F, the annual modulation of the zodiacal emission. In pointing mode of the ASTRO-F mission the spacecraft can make attitude maneuvering over approximately ${\pm}1^{\circ}$ range centered at solar elongation $90^{\circ}$ in the ecliptic plane. The attitude maneuvering combined with high sensitivity of the IRC will provide us with a unique opportunity observationally to take derivatives of the zodiacal emission brightness with respect to the solar elongation. From the resulting differential of the brightness over the ${\pm}1^{\circ}$ range, one can directly determine the mean volume emissivity of the local IPDs with a sufficient accuracy to de-modulate the annual emissivity variations due to the Earth's elliptical motion and the dis-alignment of the maximum IPD density plane with respect to the ecliptic. The non-zero eccentricity ($e_{\oplus}$= 0.0167) of the Earth's orbit combined with the sensitive temperature dependence of the Planck function would bring modulations of amplitude at least $3.34\%$ to the zodiacal emission brightness at mid-infrared wavelengths, with which one may determine the IPD temperature T(r) and mean number density n(r) as functions of heliocentric distance r. This will in turn fix the power-law exponent $\delta$ in the relation $T(r) = T_o(r/r_o)^{-\delta}$ for the dust temperature and v in $n(r) = n_o(r/r_o)^-v$ for the density. We discuss how one may de-couple the notorious degeneracy of cross-section, density, reference temperature $T_o$ and exponent $\delta$.

Health-related self-cognition and physical performance status of a elementary school children (일부 초등학교 고학년 학생들의 신체적 건강에 대한 자가-인식과 운동수행 상태)

  • Kim, Suhn-Yeop
    • The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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    • v.10 no.2
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    • pp.57-72
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    • 2004
  • Background : The part to be very important that we make the physical development rightly at elementary school student days. Objective : This study investigated the relation of a physical health status which itself recognizes and an exercise performance situation out for an elementary school students. Methods : This cross-sectional study examined health and exercise performance status of 1,032 elementary school children. To examine children's a physical injury experience and injury occurrence characteristic and a personal computer utility condition, a body alignment status of the student which parents recognize and the relation of a fatigue and concentration level was an investigation with other factors. Results: An average age of the children was the 10.95 yr and an average weight was the 39.02 kg and an average height was the 145.17cm. The student to be experiencing currently an pain occurrence was 192(19.07%). It was most high to the grade 6(23.33%) compared with different grade(p<.01). Physical pain occurrence rate did not exist by gender(p>.05). There is most an pain occurrence region the ankle(38.0%). They had a 27.19% an physical injury experience of the students. A injury rate was revealed that were high as were the high grade(grade 6, 36.72%)(p<.01). A reason of the injury rate was high in a collision at the body(19.84%), falling down(18.68%) sequence at a traffic accident(22.96%)(p<.05). There were the significant relation to the school year the reason of the injury (p<.05). Currently there was the student to be taking special exercises a 57.97%. This that they have the relation with exercise with physical pain students to recognize 23.8%. The significant relation did not exist in the physical exercise execution and pain occurrence on the body(p>.05).

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Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

  • Lee, Song Hee;Jung, Hwa Jin;Hong, Seung-Beom;Choi, Jong In;Ryu, Jae-San
    • Mycobiology
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    • v.48 no.4
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    • pp.313-320
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    • 2020
  • In Pleurotus sp., green mold, which is considered a major epidemic, is caused by several Trichoderma species. To develop a rapid molecular marker specific for Trichoderma spp. that potentially cause green mold, eleven Trichoderma species were collected from mushroom farms and the Korean Agricultural Culture Collection (KACC). A dominant fungal isolate from a green mold-infected substrate was identified as Trichoderma pleuroticola based on the sequences of its internal transcribed spacer (ITS) and translation elongation factor 1-α (tef1) genes. In artificial inoculation tests, all Trichoderma spp., including T. atroviride, T. cf. virens, T. citrinoviride, T. harzianum, T. koningii, T. longibrachiatum, T. pleurotum, and T. pleuroticola, showed pathogenicity to some extent, and the observed symptoms were soaked mycelia with a red-brown pigment and retarded mycelium regeneration. A molecular marker was developed for the rapid detection of wide range of Trichoderma spp. based on the DNA sequence alignment of the ITS1 and ITS2 regions of Trichoderma spp. The developed primer set detected only Trichoderma spp., and no cross reactivity with edible mushrooms was observed. The detection limits for the PCR assay of T. harzianum (KACC40558), T. pleurotum (KACC44537), and T. pleuroticola (CAF-TP3) were found to be 500, 50, and 5 fg, respectively, and the detection limit for the pathogen-to-host ratio was approximately 1:10,000 (wt/wt).

Clinical presentation and specific stabilizing exercise management in Lumbar segmental instability (요추분절의 불안정성에 대한 임상적 소개와 안정성 운동관리)

  • Jung Yeon-Woo;Bae Sung-Soo
    • The Journal of Korean Physical Therapy
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    • v.15 no.1
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    • pp.155-170
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    • 2003
  • Lumbar segmental instability is considered to represent a significant sub-group within the chronic low back pain population. This condition has a unique clinical presentation that displays its symptoms and movement dysfunction within the neutral zone of the motion segment. The loosening of the motion segment secondary to injury and associated dysfunction of the local muscle system renders it biomechanically vulnerable in the neutral zone. There in evidence of muscle dysfunction related to the control of the movement system. There is a clear link between reduced proprioceptive input, altered slow motor unit recruitment and the development of chronic pain states. Dysfunction in the global and local muscle systems in presented to support the development of a system of classification of muscle function and development of dysfunction related to musculoskeletal pain. The global muscles control range of movement and alignment, and evidence of dysfunction is presented in terms of imbalance in recruitment and length between the global stability muscles and the global mobility muscles. The local stability muscles demonstrate evidence of failure of aeequate segmental control in terms of allowing excessive uncontrolled translation or specific loss of cross-sectional area at the site of pathology Motor recruitment deficits present as altered timing and patterns of recruitment. The evidence of local and global dysfunction allows the development of an integrated model of movement dysfunction. The clinical diagnosis of this chronic low back pain condition is based on the report of pain and the observation of movement dysfunction within the neutral zone and the associated finding of excessive intervertebral motion at the symptomatic level. Four different clinical patterns are described based on the directional nature of the injury and the manifestation of the patient's symptoms and motor dysfunction. A specific stabilizing exercise intervention based on a motor learning model in proposed and evidence for the efficacy of the approach provided.

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Effects of Traffic Islands on Traffic Accidents Reduction (교통섬 설치로 인한 교통사고 감소 효과)

  • Gang, Dong-Su;Lee, Su-Beom;Kim, Yong-Seok;Hong, Ji-Yeon
    • Journal of Korean Society of Transportation
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    • v.28 no.2
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    • pp.21-32
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    • 2010
  • A traffic island, typical channelization facilities, is widely applied to newly road designs and black spots improvement projects as traffic safety measures. However positive effects of a traffic island for traffic safety have not been reported specifically. Accordingly this study analyzed the accidents reduction effectiveness and economics of installing traffic islands under various roadways and traffic conditions. For this purpose normality test, paired t-test and Wilcoxon signed rank test were applied to this study based on traffic accidents, roadways and traffic environments data for 3 years before and after improvement at 54 intersections. The results showed that most of traffic accidents were reduced significantly after installing traffic islands except when traffic islands were installed in outside of cities and farming regions where use efficiency is low, curve sections with poor visibility, poor alignment sections with acceleration/deceleration lanes, road sections where occurs conflicts frequently due to did not install of acceleration/deceleration lanes, and road sections where cross with single lane road.

Compact Infrared/Visible Laser Transmitter Featuring an Extended Detectable Trajectory

  • Kim, Haeng-In;Lee, Hong-Shik;Lee, Sang-Shin
    • Journal of the Optical Society of Korea
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    • v.16 no.4
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    • pp.331-335
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    • 2012
  • A miniaturized laser beam transmitter, in which a visible laser module at ${\lambda}$=650 nm is precisely stacked upon an infrared (IR) module at ${\lambda}$=905 nm, has been proposed and constructed to provide an IR collimated beam in conjunction with a collinear monitoring visible beam. In particular, the IR beam is selectively dispersed through a perforated sheet diffuser, so as to create a rapidly diverging close-range beam in addition to a highly defined long-range beam simultaneously. The complementary close-range beam plays a role in mitigating the blind region in the vicinity of the transmitter, which is inevitably missed by the main long-range beam, thereby uniformly extending the transmitter's effective trajectory that is sensed by a receiver. The proposed transmitter was designed through numerical simulations and then fabricated by incorporating a diffuser sheet, perforated with an aperture of 2 mm. For the manufactured transmitter, the IR long-range beam was observed to have divergences of ~2.3 and 1.6 mrad in the fast and slow axes, respectively, while the short-range beam yielded a divergence of ~24 mrad. The angular alignment between the long-range IR and visible beams was as accurate as ~0.5 mrad. According to an outdoor feasibility test involving a receiver, the combination of the IR long- and short-range beams was proven to achieve a nearly uniform trajectory over a distance ranging up to ~600 m, with an average detectable cross-section of ${\sim}60{\times}80cm^2$.

DEVELOPMENT OF LIGHTWEIGHT OPTICAL TELESCOPE KIT USING ALUMINUM PROFILE AND ISOGRID STRUCTURE

  • Park, Woojin;Lee, Sunwoo;Han, Jimin;Ahn, Hojae;Ji, Tae-Geun;Kim, Changgon;Kim, Dohoon;Lee, Sumin;Kim, Young-Jae;Kim, Geon-Hee;Kim, Junghyun;Kim, Ilhoon;Pak, Soojong
    • Journal of The Korean Astronomical Society
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    • v.55 no.1
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    • pp.11-22
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    • 2022
  • We introduce the Transformable Reflective Telescope (TRT) kit that applies an aluminum profile as a base plate for precise, stable, and lightweight optical system. It has been utilized for optical surface measurements, developing alignment and baffle systems, observing celestial objects, and various educational purposes through Research & Education projects. We upgraded the TRT kit using the aluminum profile and truss and isogrid structures for a high-end optical test device that can be used for prototyping of precision telescopes or satellite optical systems. Thanks to the substantial aluminum profile and lightweight design, mechanical deformation by self-weight is reduced to maximum 67.5 ㎛, which is an acceptable misalignment error compared to its tolerance limits. From the analysis results of non-linear vibration simulations, we have verified that the kit survives in harsh vibration environments. The primary mirror and secondary mirror modules are precisely aligned within 50 ㎛ positioning error using the high accuracy surface finished aluminum profile and optomechanical parts. The cross laser module helps to align the secondary mirror to fine-tune the optical system. The TRT kit with the precision aluminum mirror guarantees high quality optical performance of 5.53 ㎛ Full Width at Half Maximum (FWHM) at the field center.