• Korean Journal of Medicinal Crop Science
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• v.16 no.6
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• pp.446-454
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• 2008

An advanced extraction method by ultrasonic extraction with applied solid phase extraction (SPE) has been developed for the determination of simultaneous eight major ginsenosides, namely ginsenosides Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, and Rd in the root of Panax ginseng. Four extraction methods including n-BuOH reflux extraction (Method A), 70% EtOH reflux extraction (Method B), 50% MeOH reflux extraction with SPE (Method C), and 50% MeOH ultrasonication with SPE clean-up process (Method D) were investigated for the determination of eight major ginsenosides. Total contents of ginsenosides were highest by extraction of Method C as $2.408{\pm}0.011%$. However, Method D was evaluated as relatively simpler and more efficient method due to short extraction time, small solvent consumption and less expensive, compared to conservative reflux method. Ginsenosides were also satisfactorily separated with good resolution and the accuracy range was between 1.05 and 4.06% as relative standard deviation (RSD) by Method D. SPE condition and HPLC condition were further optimized for determination of eight major ginsenosides by the ultrasonic extraction method. Conclusively, ultrasonic extraction of 2 g sample of ginseng using ultrasonic bath and 1 loading for SPE was evaluated as proper condition for extraction of ginseng.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.2
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• pp.112-119
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• 2018

Heat stress during the ripening stage reduces the yield and quality of rice. Considering the adverse effects of global warming, it is necessary to breed heat stress tolerant rice cultivars and analyze their stress tolerance characteristics. We investigated the ripening characteristics and antioxidant enzyme activity of Ilmi under heat stress condition during the ripening stage. Ripening rate, 1000 brown grain weight, and rice quality of Ilmi were not significantly changed by heat stress during the ripening stage. Leaf chlorophyll, chlorophyll a/b ratio, and malondialdehyde (MDA) contents of Ilmi leaves were also less changed than those of Ilpum. These results strongly suggested that Ilmi has heat tolerance characteristics during the ripening stage. Analysis of antioxidant enzyme activity of Ilmi revealed that peroxidase (POX) activity was significantly higher than that of Ilpum, and showed a significant correlation with the change in chlorophyll a/b ratio and hydrogen peroxide content of flag leaves of Ilmi. These data suggest that the high POX enzyme activity of Ilmi could be considered one of its major heat tolerance characteristics.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.60-60
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• 2017

It is important to measure the gas exchange activity of the crops in canopy scale to understand the process of biomass production and yield formation. Thermal imaging of the canopy surface temperature is a powerful tool to detect the gas exchange activity of the crop canopy. The simultaneous measurement of the canopy temperature and the meteorological data enables us to calculate the canopy diffusive conductance ($g_c$) based on the heat flux model (Monteith et al. 1973, Horie et al. 2006). It is, however, difficult to realize the long-term and continuous monitoring of $g_c$ due to the occurrence of the calculation error caused by the fluctuation of the environmental condition. This is partly because the model assumption is too simple to describe the meteorological and aerodynamic conditions of the crop canopy in the field condition. Here we report the novel method of the direct measurement of the aerodynamic resistance ($r_a$) of the crop canopy, which enables us the stable and continuous measurement of the gas exchange capacity of the crop plants. The modified heat balance model shows the improved performance to quantify $g_c$ under the fluctuating meteorological condition in the field. The relationship between $g_c$ and biomass production of rice and soybean varieties is also discussed in the presentation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.5
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• pp.395-400
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• 2001

We investigated the variations of the flowering response to daylength in Perilla crop (var. frutescens). Seventeen accessions of Perilla crop and one accession of weedy type of var. crispa from China, Korea and Japan were cultivated under three daylength conditions, i.e., short-days, natural daylength and long-days. Most accessions of Perilla crop from China, Korea and Japan were divided into three types, early maturing type, intermediate maturing type and late maturing type by their natural flowering habit. In most of the accessions used, the flowering habit was significantly accelerated by short-day conditions and was delayed by long-day conditions. All the accessions of Perilla crops flowered within 57 days under the 10 hrs light treatment, whereas they did not flower at all even at 170 days after sowing under the 16 hrs light treatment. Thus, this finding suggested that there is a relationship between the types of flowering response to daylength and the geographical distribution which determines the planting season in traditional cultivation practices of Perilla crops. Positive correlation was observed between days to flowering and plant height or internode number in both the short-day and natural daylength conditions. Whereas, correlation was negative between days to flowering and inflorescence length or floret number in natural daylength condition, but it was positive in the short-day condition. Therefore, the daylength condition is considered as the most important environmental factor for flowering habit and morphological characters of Perilla crops. Flowering habit is considered as an important key character for the study of geographical differentiation of Perilla crop in East Asia.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.3
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• pp.240-244
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• 1993

Yacon(Polymina sonchifolia POEPP), an indigenous Andean natural resource food plant, was imported as a new root crop from New Zealand in 1986. However the chemical composition and planting system of it have not been research in Korea. The experimental results for the optimum planting densities and mulching effect are as follows. Height of the main stem of yacon was grown linear from July to October, is showed more fast growth as density increased, and showed highest in 70 ${\times}$ 55cm density. The tiller was bubed 3 month after planting but there was no significant difference among mulching, non-mulching condition and planting density. The fresh weight of root of mulching condition was heavier than that in non-mulching condition and 70 ${\times}$ 40cm mulching condition. In mulching treatment, number of roots in dense planting were more than that in sparse planting. The effect of mulching was not shown significantly in root diameter and root length.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.4
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• pp.327-332
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• 2010

This study was conducted to overcome the deliciousness by sex reversal in hemp plants. When female plants grown under long day length condition were transferred to short day length condition in 3th and 6th leaf stage, a few male flowers were formed and the gap of flowering days between sex reversed male and normal female flowers were around 10 days and 3 days, respectively. Although the flowering amounts were small, this short gaps of flowering days were enough for artificial pollination. When female plants grown under long day length condition were transferred to short day length condition just after treatment of silver nitrate in the vegetative stage, a great amount of male flower was formed and the gap of flowering days between sex reversed male and normal female flowers were only 1 to 3 days. The increased sex reversed male flowers and induced short gap of flowering days were adequate for natural wind pollination. When female seeds were cultivated, seed yield was 141 kg/10a, while it was 96 kg/10a when normal seeds cultivated.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.s01
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• pp.26-32
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• 1989

The analytic principles of GC and MS were explained in relation to plant hormone analyses and the characteristics of two instruments were compared. The selection of column, condition of measurement and the method of ionization to get a good spectrum were also briefly described. Finally, the pre-treatment of sample by solvent extraction method to remove the unnecessary part of sample and the synthetic method, especially reagents and reaction condition, for the preparation of ether or ester derivative which can be easily vaporized in GC were explained.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.296-297
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• 2000

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.430-435
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• 2012

Low silage corn yield due to bad soil physical properties in the paddy field converted to upland condition is the chief obstacles to expanding the area for silage maize production. The effect of subsoiling (subsoiling to depth 40 cm) on soil physical properties and silage corn yield were investigated in the first year of paddy field converted to upland condition in 2010 and 2011, respectively. Soil compaction was loosened much particularly at depth 25~35cm as much as 1~1.5 MPa and soil bulk density and porosity at depth 15~30 cm are improved by subsoiling. Maize growth was increased by subsoiling, particularly in kernel number per ear which increased ear weight. Total digestible nutrients (TDN) yield of silage maize was increased as much as 19 and 39% in 2010 and 2011, respectively showing that yield increase according to subsoiling was higher when maize growth was prohibited much by excess-moisture injury due to heavy rain in 2011.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.114-122
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• 2015

Soybean is very useful crop to supply vegetable protein for human. However, cultivation arear of this economically important crop is gradually diminished in upland field. Hence, cultivation area of soybean is increased in paddy field. During the growth duration of soybean, excessive moisture injury is serious problem for sustainable production and supply. We investigated protein expression according to different period of seed soaking and germination after seed soaking. For comparison on expression of protein according to different condition, we performed two-dimensional electrophoresis. After electrophoresis analysis, we selected differentially expressed protein spots according to different condition such as soaking period and germination after soaking to identify protein function by using MALDI-TOF. Results revealed that pattern of expression of protein according to soaking period and germination after soaking were generally not different in major spots. However, degree of expression of protein in some protein spots was increased in accordance with decrease of soaking period. Especially, in Hwangkeum-Kong, Danyeop-Kon, and Pecking, the degree of expression of protein was remarkably increased for 4 days after soaking. But, according to germination after soaking, degree of expression of protein in germinated seeds of all cultivars was higher than un-germinated seeds. In results of MALDI-TOF analysis, specific proteins were identified by different soaking period such as Allergen Gly m Bd 28K, P24 oleosin isoform B. Also, in accordance with germination, degree of protein expression of the related protein, Gibberellin was increased in un-germinated seeds of Iksan-Kong. In ungerminated seeds of Sinpaldal-kong, proteins were identified as down-regulated by soaking such as ATP binding and Inhibitor II', proteinase.