Characterization of Protein Function and Differential Protein Expression in Soybean under Soaking Condition

Soybean is very useful crop to supply vegetable protein for human. However, cultivation arear of this economically important crop is gradually diminished in upland field. Hence, cultivation area of soybean is increased in paddy field. During the growth duration of soybean, excessive moisture injury is serious problem for sustainable production and supply. We investigated protein expression according to different period of seed soaking and germination after seed soaking. For comparison on expression of protein according to different condition, we performed two-dimensional electrophoresis. After electrophoresis analysis, we selected differentially expressed protein spots according to different condition such as soaking period and germination after soaking to identify protein function by using MALDI-TOF. Results revealed that pattern of expression of protein according to soaking period and germination after soaking were generally not different in major spots. However, degree of expression of protein in some protein spots was increased in accordance with decrease of soaking period. Especially, in Hwangkeum-Kong, Danyeop-Kon, and Pecking, the degree of expression of protein was remarkably increased for 4 days after soaking. But, according to germination after soaking, degree of expression of protein in germinated seeds of all cultivars was higher than un-germinated seeds. In results of MALDI-TOF analysis, specific proteins were identified by different soaking period such as Allergen Gly m Bd 28K, P24 oleosin isoform B. Also, in accordance with germination, degree of protein expression of the related protein, Gibberellin was increased in un-germinated seeds of Iksan-Kong. In ungerminated seeds of Sinpaldal-kong, proteins were identified as down-regulated by soaking such as ATP binding and Inhibitor II', proteinase.

Proteomics를 이용한 콩의 발아 전 침종처리에 따른 단백질 발현 양상 비교 분석

침종 기간과 침종 후 발아의 유무에 따른 단백질 발현을 이차원전기영동을 이용하여 단백질 발현양상을 확인하고 비교 분석한 결과, 침종 기간과 침종 후 발아의 유무에 따른 단백질 발현 양상은 전반적으로 매우 유사하였으며, 주요 단백질의 발현에는 차이가 없었다. 침종 기간에 따른 품종별 단백질 발현 양상은 침종 기간이 길어짐에 따라 단백질 발현정도가 점차 증가되는 것을 확인할 수 있었다. 공시품종들 중 황금콩, 단엽콩, Peking이 다른 품종들에 비하여 침종 4일후에 단백질 발현정도가 급격히 증가하는 것을 알 수 있었다. 침종 후 발아 유무에 따른 단백질 발현양상은 소수의 단백질 spot들을 제외하고는 전반적으로 모든 공시 품종들에서 발아한 종자에서의 단백질 발현정도는 미발아 종자에서보다 높게 확인되었다.