Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Extraction and Preprocessing Methods for Ginsenosides Analysis of Panax ginseng C.A. Mayer

인삼의 진세노사이드 분석을 위한 추출 및 전처리법

  • Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hyun, Dong-Yun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Young-Ock (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Sung-Woo (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Young-Chang (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Son, Yeong-Deck (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Min-Jeong (KT&G Central Research Institute) ;
  • Park, Chung-Berm (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Ho-Ki (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cha, Seon-Woo (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA) ;
  • Song, Kyung-Sik (College of Agriculture and Life Science, Kyungpook National University)
  • 김금숙 (농진청 국립원예특작과학원 인삼특작부) ;
  • 현동윤 (농진청 국립원예특작과학원 인삼특작부) ;
  • 김영옥 (농진청 국립원예특작과학원 인삼특작부) ;
  • 이성우 (농진청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농진청 국립원예특작과학원 인삼특작부) ;
  • 이승은 (농진청 국립원예특작과학원 인삼특작부) ;
  • 손영득 (농진청 국립원예특작과학원 인삼특작부) ;
  • 이민정 (KT&G중앙연구원) ;
  • 박충범 (농진청 국립원예특작과학원 인삼특작부) ;
  • 박호기 (농진청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농진청 국립원예특작과학원 인삼특작부) ;
  • 송경식 (경북대학교 생명과학대학)
  • Published : 2008.12.30
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Abstract

An advanced extraction method by ultrasonic extraction with applied solid phase extraction (SPE) has been developed for the determination of simultaneous eight major ginsenosides, namely ginsenosides Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, and Rd in the root of Panax ginseng. Four extraction methods including n-BuOH reflux extraction (Method A), 70% EtOH reflux extraction (Method B), 50% MeOH reflux extraction with SPE (Method C), and 50% MeOH ultrasonication with SPE clean-up process (Method D) were investigated for the determination of eight major ginsenosides. Total contents of ginsenosides were highest by extraction of Method C as $2.408{\pm}0.011%$. However, Method D was evaluated as relatively simpler and more efficient method due to short extraction time, small solvent consumption and less expensive, compared to conservative reflux method. Ginsenosides were also satisfactorily separated with good resolution and the accuracy range was between 1.05 and 4.06% as relative standard deviation (RSD) by Method D. SPE condition and HPLC condition were further optimized for determination of eight major ginsenosides by the ultrasonic extraction method. Conclusively, ultrasonic extraction of 2 g sample of ginseng using ultrasonic bath and 1 loading for SPE was evaluated as proper condition for extraction of ginseng.

Keywords

References

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