• Korean journal of applied entomology
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• v.54 no.1
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• pp.7-15
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• 2015

CpBV (Cotesia plutellae bracovirus) is a polydnavirus and encodes a cystatin (CpBV-CST1) gene. Its overexpression suppresses insect immunity and alters insect developmental processes. This study aimed to construct a genetically modified (GM) tobacco to further explore the physiological function of the viral cystatin and to apply to control insect pests. To this end, the transgenic tobacco lines were screened in expression of the target gene and assessed in insecticidal activity. A recombinant vector (pBI121-CST) was prepared and used to transform a bacterium, Agrobacterium tumefasciens. The transformed bacteria were used to generate transgenic tobacco lines, which were induced to grow callus and resulted in about 92% of shoot regeneration. The regenerated plants were screened by PCR analysis to confirm the insertion of the target gene in the plant genome. In addition, the expression of the target gene was assessed in the regenerated plants by quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis showed that the transgenic line plant expressed the target gene about 17 times more than the control tobacco, indicating a stable insertion and expression of the target gene in the transgenic tobacco line. The insecticidal activity was then analyzed using the screened transgenic tobacco lines against the teneral 1st instar larvae of the oriental tobacco budworm, Helicoverpa assulta. Though there was a variation in the insecticidal efficacy among transgenic lines, T9 and T12 lines exhibited more than 95% mortality at 7 days after feeding treatment. These results suggest that CpBV-CST1 is a useful genetic resource to be used to generate GM crop against insect pests.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.218-222
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• 2006

This experiment was conducted to investigate the effects of climatic factors varied due to the type of plastic house, cultural season and location in the house on the growth of cucumber plants grown by nutrient solution. There were two growing periods, summer culture and retarding culture, two types of plastic houses, 1-2W type house and post-less house. Air temperature, relative humidity and amount of solar radiation in the plastic houses were measured. Also, dry weight of leaves and stems, plant height, number of leaves per plant, leaf area per plant and fresh weight of fruits per plant were observed. Plant growth analysis were conducted and interrelationships between climatic factors and physiological characteristics were investigated. The results were as follows. There were no differences between the type of plastic houses in the average air temperature and average relative humidity in the plastic house, but amount of solar radiation in 1-2W type house was significantly higher than that of postless house. Daily cumulative solar radiation were highest in southwest side of 1-2W type house and northwest side of postless house. Plant height and number of leaves per plant were higher in summer culture than retarding culture, while leaf area per plant was higher in retarding culture than summer culture. Relative growth rate (RGR) showed highly significantly positive correlations with net assimilation rate (WAR) and leaf area ratio (LAR). Contribution of NAR to RGR was much higher than that of LAR. Crop growth rate (CGR) showed highly significantly positive correlations with leaf area index (LAI). It appeared that increase of LAI was important to increase productivity of cucumber. Average daily air temperature for the whole growing period showed highly significantly positive correlations with RGR and NAR. Furthermore, cumulative solar radiation for the whole growing period in retarded culture showed significantly positive correlation with RGR and NAR.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.782-789
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• 2013

Carrot (Daucus carota L. var. sativa) is one of the most widely used crops in the world and is nutritionally important crop. However, seed-hair which is generated in epidermal cell of seeds causes the difficulty of the seedling process, because of the seed germination and absorption inhibitions. For these reasons, carrot seeds are commercialized after mechanical hair removal process. However, in this process, various damage and seed loss occur and breeding of hairless-seed carrot cultivar is needed to overcome these various weaknesses and additional seed production costs. In this study, cDNA libraries using 2 combinations, which were composed of short-hair seed CT-ATR 615 OP 666-13 & long-hair seed CT-ATR 615 OP 671-9, and short-hair seed CT-SMR 616 OP 659-1 & long-hair seed CT-SMR 616 OP 677-14, were constructed and EST sequences of each individuals were analyzed to reveal carrot seed-hair characteristics. Firstly, analyzed EST sequences were classified into FunCat functional categories. As a result, significant differences have been identified in metabolism category, protein folding and stabilization, protein binding, C-compound binding category from both of two combinations. Secondly, several candidate EST sequences related to seed trichome differentiation and cellulose biosynthetic process were selected based on GO data of EST sequences. These differences based on FunCat categories and candidate EST obtained by GO data analysis are thought to be involved in the formation of carrot seed hair. Finally, 741 SSR sites and 33 SNP sites were identified from analyzed EST sequences of two combinations. Then we designed SNP and SSR primer sets to develop molecular markers. These molecular markers will be used for classification of carrot cultivars and study seed-hair characteristic.

• Journal of Bio-Environment Control
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• v.24 no.2
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• pp.93-99
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• 2015

The purpose of this study was to determine the appropriate temperature for water curtain in greenhouses equipped with recirculated water curtain system. The study analyzed the changes in air temperature in non-heated greenhouses for strawberry cultivation based on outdoor temperature, water curtain temperature and night time. Three greenhouse units were used for this study: The first unit was assigned as a control (no water curtain system), two other greenhouses were equipped with recirculated water curtain system with water curtain temperatures of $10^{\circ}C$ and $15^{\circ}C$, respectively. Analysis showed that the indoor temperatures were directly correlated with the outdoor temperature in all experimental greenhouses. Heat insulating effect of $15^{\circ}C$ water curtain was increased by $1.3^{\circ}C$ compared to that in $10^{\circ}C$ water curtain system. The $15^{\circ}C$ water curtain treatment showed the highest average temperature and less temperature variation in comparison with control and $10^{\circ}C$ water curtain treatment. To maintain indoor temperature at $5^{\circ}C$, water curtain temperature of $10^{\circ}C$ was suitable when outdoor minimum and average temperatures were -1.3 and $1.5^{\circ}C$, and water curtain temperature of $15^{\circ}C$ was suitable when outdoor minimum and average temperatures were -4.7 and $-0.2^{\circ}C$, respectively. The highest temperature in greenhouses according to measurements in different periods of night time was observed after sunset (18:30-20:30), and the lowest temperature before sunrise (05:00-07:00). Water curtain maintained a target indoor temperature by acting as a layer of heat transfer insulator which decreased heat loss from greenhouses. Therefore, water temperature in recirculating water curtain systems should be determined by considering outdoor temperatures, changes in temperature at different periods of night time, and cultivated crop.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.510-523
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• 2020

This study analyzed groundwater quality in hydroponic cultivation facilities. Through this study, the possibility of groundwater use was evaluated according to the quality of the groundwater for hydroponic cultivation facilities. Good groundwater quality, on average, is pH 6.61, EC 0.27 dS/m, NO3-N 7.64 mg/L, NH4+-N 0.80 mg/L, PO4-P 0.09 mg/L, K+ 6.26 mg/L, Ca2+ 18.57 mg/L, Mg2+ 4.38 mg/L, Na+ 20.85 mg/L, etc. All of these satisfy the water quality standard for raw water in nutrient cultivation. But in the case of farmers experiencing problems with groundwater quality, most of the items exceeded the water quality standard. As a result of the analysis, it was judged that purifying groundwater of unsuitable quality for crop cultivation, and using it as raw water, was effective in terms of water quality and soil purification. If an agricultural water purification system is constructed based on the results of this study, it is judged that the design will be easy because the items to be treated can be estimated. If a purification system is established, it can use groundwater directly in the facility and for horticulture. These study results will be available for use in sustainable agriculture and environments.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.384-392
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• 2022

The objective of this study was to estimated nitrogen content and chlorophyll using RGB, Hyperspectral sensors to diagnose of nitrogen nutrition in apple tree leaves. Spectral data were acquired through image processing after shooting with high resolution RGB and hyperspectral sensor for two-year-old 'Hongro/M.9' apple. Growth data measured chlorophyll and leaf nitrogen content (LNC) immediately after shooting. The growth model was developed by using regression analysis (simple, multi, partial least squared) with growth data (chlorophyll, LNC) and spectral data (SPAD meter, color vegetation index, wavelength). As a result, chlorophyll and LNC showed a statistically significant difference according to nitrogen fertilizer level regardless of date. Leaf color became pale as the nutrients in the leaf were transferred to the fruit as over time. RGB sensor showed a statistically significant difference at the red wavelength regardless of the date. Also hyperspectral sensor showed a spectral difference depend on nitrogen fertilizer level for non-visible wavelength than visible wavelength at June 10th and July 14th. The estimation model performance of chlorophyll, LNC showed Partial least squared regression using hyperspectral data better than Simple and multiple linear regression using RGB data (Chlorophyll R²: 81%, LNC: 81%). The reason is that hyperspectral sensor has a narrow Full Half at Width Maximum (FWHM) and broad wavelength range (400-1,000 nm), so it is thought that the spectral analysis of crop was possible due to stress cause by nitrogen deficiency. In future study, it is thought that it will contribute to development of high quality and stable fruit production technology by diagnosis model of physiology and pest for all growth stage of tree using hyperspectral imagery.

• Journal of Bio-Environment Control
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• v.31 no.3
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• pp.246-254
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• 2022

Domestic facility agriculture grows rapidly, such as modernization and large-scale. And the production scale increases significantly compared to the area, accounting for about 60% of the total agricultural production. Greenhouses require energy input to create an appropriate environment for stable mass production throughout the year, but the energy load per unit area is large because of low insulation properties. Through the rooftop greenhouse, one of the types of urban agriculture, energy that is not discarded or utilized in the building can be used in the rooftop greenhouse. And the cooling and heating load of the building can be reduced through optimal greenhouse operation. Dynamic energy analysis for various environmental conditions should be preceded for efficient operation of rooftop greenhouses, and about 40% of the solar energy introduced in the greenhouse is energy exchange for crops, so it should be considered essential. A major analysis is needed for each sensible heat and latent heat load by leaf surface temperature and evapotranspiration, dominant in energy flow. Therefore, an experiment was conducted in a rooftop greenhouse located at the Korea Institute of Machinery and Materials to analyze the energy exchange according to the growth stage of crops. A micro-meteorological and nutrient solution environment and growth survey were conducted around the crops. Finally, a regression model of leaf temperature and evapotranspiration according to the growth stage of leafy vegetables was developed, and using this, the dynamic energy model of the rooftop greenhouse considering heat transfer between crops and the surrounding air can be analyzed.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.423-431
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• 2022

Due to increase of interest in 'carbon neutrality', attempts at agricultural use of CO₂ are increasing. In this study, we used the dry-ice made by CO₂ as by-product in thermoelectric power plant on CO₂ fertilization for production of leafy vegetable in greenhouses. The dry-ice was supplied on three leafy vegetable farms (Allium tuberosum Rottl. ex Spreng, Aster scaber, and Oenanthe stolonifera DC.) located in Hadong, Gyeongsangnamdo. Two greenhouses were used in each leaf vegetable crops, one greenhouse used as the control (non-treatment), other greenhouse used as supplied CO₂. For CO₂ fertilization, a gas sublimated from dry ice was supplied to the greenhouse using a specially designed prototype supply machine. A. tuberosum greenhouse has no difference of CO₂ concentration between the control, and CO₂ fertilization and shown high CO₂ concentration both greenhouses. However, the CO₂ concentrations in A. scaber and O. stolonifera greenhouses were increased in CO₂ fertilization treatment. The growth of A. scaber and O. stolonifera were increased in CO₂ fertilization, and the yield also increased to 36% and 25% than the control, respectively. As a result of economic analysis, the A. scaber has increase of income rate, however A. tuberosum and O. stolonifera has decreased income rate. Thus, the use of the dry-ice made by CO₂ as by-product in thermoelectric power plant has possibility to increase productivity of the leafy vegetable in greenhouse and have agricultural use value.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.559-572
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• 2021

A survey was conducted to analyze water masses and spatial distributions of phytoplankton communities at 15 stations on the surface and chlorophyll a maximum layers (CML) in the western South Sea of Korea from September 8 to 9, 2021. As a result, water masses were classified into Coastal Waters (CW) with relatively low salinity, the Tsushima Warm Current (TWC) with high water temperature and high salinity, and mixed waters (MW) showing a mixture of these two water masses. Turbidity showed high concentration in both the surface and CML. The chlorophyll a concentration was as low as 0.90±0.43 ㎍ L^-1 in the surface, more than 1.1 ㎍ L^-1 in CW, around 1.0 ㎍ L^-1 in MW, and less than 0.5 ㎍ L^-1 in the TWC. CML was 1.64±0.54 ㎍ L^-1. Regarding species composition of phytoplankton communities, there were 57 species in 31 genera(diatoms, 57.8%; dinoflagellates, 35.1%; and other phytoflagellates, 7.1%). The phytoplankton standing crop had 4.6±7.6 cells mL^-1 in the surface, more than 30 cells mL^-1 in the CW, 2-5 cells mL^-1 in the MW, and less than 2 cells mL^-1 in the TWC. CML was slightly higher than the surface with a variation of 5.7±8.4 cells mL^-1. Dominant species were found to be Rhizosolenia flagilissima f. flagilissima, Skeletonema costatum-ls, and Nitzschia sp./ small size in the surface. For the CML Rh. flagilisima f. flagilissima showed a dominance of 12.0%. For the surface, the diversity variation was 2.36±0.40, which was high for TWC but low for MW. For CML, the diversity variation was 2.29±0.52, which was slightly lower than that of the surface. The dominance in the surface was 0.50±0.15, with a fluctuation range of more than 0.5 in MW and less than 0.5 in the TWC, which was different from the diversity. According to correlation analysis and principal component analysis (PCA), the presence of phytoplankton standing crops was high in CW but low in MW and TWC. That is, phytoplankton communities in early autumn were strongly affected by the expansion and mixing of water masses in western South Sea.

• Journal of Bio-Environment Control
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• v.32 no.2
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• pp.106-114
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• 2023

With rising concerns about pesticide spray drift by aerial application, this study attempt to evaluate aerodynamic property and collection efficiency of spray drift according to the leaf area index (LAI) of crop for preventing undesirable pesticide contamination by the spray-drift tunnel experiment. The collection efficiency of the plant with 'Low' LAI was measured at 16.13% at a wind speed of 1 m·s^-1. As the wind speed increased to 2 m·s^-1, the collection efficiency of plant with the same LAI level increased 1.80 times higher to 29.06%. For the 'Medium' level LAI, the collection efficiency was 24.42% and 43.06% at wind speed of 1 m·s^-1 and 2 m·s^-1, respectively. For the 'High' level LAI, it also increased 1.24 times higher as the wind speed increased. The measured results indicated that the collection of spray droplets by leaves were increased with LAI and wind speed. This also implied that dense leaves would have more advantages for preventing the drift of airborne spray droplets. Aerodynamic properties also tended to increase as the LAI increased, and the regression analysis of quadric equation and power law equation showed high explanatory of 0.96-0.99.