• Parasites, Hosts and Diseases
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• v.39 no.2
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• pp.177-183
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• 2001

The objectives of the present study were to (1) determine the susceptibility of Anopheles sinensis to Korean isolates of Plasmodium vivax, (2) establish a method to collect large quantities of P. vivax sporozoites for use as antigen in seroepidemiological studies, and (3) investigate the characteristics of Korean isolates of P. vivax sporozoites. Females of Anopheles sinensis were collected at non-epidemic area, Seokwha-ri, Cheongwon-gun and Chungcheongbuk-do using tent-trap methods coupled with dry ice. The females were artificially infected with gameiocytes of P. vivax using blood obtained from P vivax malaria patients. Individual mosquitoes were infected using either a parafilm-covered glass feeding apparatus or were allowed to feed on naturally infected volunteers. Mosquitoes were sacrificed between 16 and 18 days post-feeding and an enzyme-linked immunosorbent assay (ELISA) was used to detect sporozoites. Four (33.4%) of 12 mosquitoes, which were fed on naturally infected volunteers directly, were positive for sporozoites. In cases, the mosquitoes allowed to feed on whole blood which were extract from three different patients with heparin treated vacuutainers using a parafilm-covered glass apparatus. Two of 55 (3.6%) were positive which blood sample was maintained at room temperature for 8 hours, 1 of 68 (1.5%) was positive which blood was maintained at $4^{\circ}C$ for 24 hours and 1 of 47 (2.3%) was positive at 4$^{\circ}C$ for 48 hours. The mean number of sporozoites was estimated about 818 (n=8; range of 648-1,056) based on optical density values of ELISA.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.1
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• pp.43-51
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• 2002

The cloning and expression of $\beta-glucosidase$ II, encoded by the gene ${\beta}glu2$, from thermotolerant yeast Pichia etchellsii into Escherichia coli is described. Cloning of the 7.3 kb BamHI/SalI yeast insert containing ${\beta}glu2$ in pUC18, which allowed for reverse orientation of the insert, resulted in better enzyme expression. Transformation of this plasmid into E. coli JM109 resulted in accumulation of the enzyme in periplasmic space. At $50^{\circ}C$, the highest hydrolytic activity of 1686 IU/g protein was obtained on sophorose. Batch and fed-batch techniques were employed for enzyme production in a 14 L bioreactor. Exponential feeding rates were determined from mass balance equations and these were employed to control specific growth rate and in turn maximize cell growth and enzyme production. Media optimization coupled with this strategy resulted in increased enzyme units of 1.2 kU/L at a stabilized growth rate of $0.14\;h^{-l}$. Increased enzyme production in bioreactor was accompanied by formation of inclusion bodies.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.8
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• pp.830-838
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• 2003

In this paper, TX/RX dual operation microstrip single antenna for satellite communication is designed, analyzed, fabricated and measured. TX/RX frequency ranges are 14.0∼l4.5 GHz, 11.7∼12.75 GHz in respectively and vertical and horizontal polarizations are used for TX and RX. This antenna uses microstrip direct feeding for RX and aperture coupled strip-line feeding for TX and accommodates stacked elements for a high directivity and wide impedance bandwidth. In an analysis of single element, FDTD and MOM was compared and FDTD analysis was more accurate because of the consideration of finite structure and imperfect two ground planes. The proposed structure facilitates generally to an extension of two dimensional array and lower an unwanted radiation by strip-line feed in TX. TX/RX 8${\times}$4 array has a return loss below -10 dB, -14 dB in TX, RX respectively and a gain ranging from 19.1∼20.7 dB in TX, 21.2∼21.8 dB in RX which has a radiation efficiency of 43∼5l %, 52∼57 %.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.16 no.9 s.100
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• pp.872-882
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• 2005

In this paper, we examined the operating principle of a passive tag antenna for RFID system in UHF band. Based on the study, we proposed a novel RFID tag antenna which adopts the inductively coupled feeding structure to match antenna impedance to a capacitively loaded commercial tag chip. The proposed tag antenna consists of microstrip lines on a thin PET substrate for low-cost fabrication. The detail structure of the tag antenna were optimized using a full electromagnetic wave simulator of IE3D in conjunction with a Pareto genetic algorithm and the size of the tag antenna can be reduced up to kr=0.27($2 cm^2$). We built some sample antennas and measured the antenna characteristics such as a return loss, an efficiency, and radiation patterns. The readable range of the tag antenna with a commercial RFID system showed about 1 to 3 m.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.11
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• pp.84-90
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• 2010

In this paper, the reduced size of broad bandpass filter with parallel coupled line structure is presented. This proposed filter can control bandwidth of narrow to broadband by adjusting the coupling coefficient. The conventional filter is operated with a narrow band. If a higher bandwidth is desired than the conventional narrow bandwidth, it is hard to realize due to the coupling coefficient between feeding line and resonator. In this paper, to overcome this limitation, a proposed bandpass filter is designed with reduced size due to SIR(Stepped Impedance Resonator) and multi-grade type structures than conventional one, and it has characteristics of adjusting bandwidth freely as per quantity of coupling coefficient. The proposed bandpass filter that, experimental results of insertion and return losses are 0.42 dB and 20.9 dB with bandwidth of 60 % at the center frequency of 5.8 GHz, respectively.

• Journal of the Korean Institute of Telematics and Electronics D
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• v.36D no.12
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• pp.11-20
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• 1999

We have designed and fabricated direct-and gap-coupled microstrip hairpin-comb filters by patterning double-sided YBCO films on a single 50-mm-diameter, 0.5-mm-thick $LaAlO_3$ wafer. Both filters have a center frequency at 1.773 GHz, 12 MHz bandwidth, 0.5 dB minimum insertion loss in the passband, and very strong out-of- band rejection. Due to two attenuation poles below and above the passband, the direct-coupled hairpin-comb filter showed a better skirt characteristic than the gapcoupled hairpin-comb filter which had only one attenuation pole below the passband.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.5
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• pp.705-712
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• 2001

In this paper, a new type of aperture coupled microstrip patch antenna with tilted-beam based on the principal of the dipole yagi antenna is proposed and investigated experimentally. Its configuration is composed by 3 types of patches; reflector, driver, and director. Tilted beam patterns are effected by many parameters as those of dipole yagi antenna; sizes of the patches, gaps between the patches, characteristics of the substrates, feeding method and etc. Therefore, in this paper, the effects of varying design parameters of this antenna are studied with a goal of enhancing the gain and tilting the beams. A microtrip patch antenna with tilted beam based on performance trade-offs is designed and fabricated. Measured and simulated results for return loss and radiation patterns are presented. It has 45$^{\circ}$ tilted beam and very close to simulation beam pattern at resonant frequency, 2.58 GHz.

• Progress in Superconductivity and Cryogenics
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• v.18 no.1
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• pp.41-45
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• 2016

The technology of supplying the electric power by wireless power transfer (WPT) is expected for the next generation power feeding system since it can supply the power to portable devices without any connectors through large air gap. As such a technology based on strongly coupled electromagnetic resonators is possible to deliver the large power and recharge them seamlessly; it has been considered as a noble option to wireless power charging system in the various power applications. Recently, various HTS wires have now been manufactured for demonstrations of transmission cables, motors, MAGLEV, and other electrical power components. However, since the HTS magnets have a lower index n value intrinsically, they are required to be charged from external power system through leads or internal power system. The portable area is limited as well as the cryogen system is bulkier. Thus, we proposed a novel design of wireless power charging system for superconducting HTS magnet (WPC4SM) based on resonance coupling method. As the novel system makes possible a wireless power charging using copper resonance coupled coils, it enables to portable charging conveniently in the superconducting applications. This paper presented the conceptual design and operating characteristics of WPC4SM using different shapes' copper resonance coil. The proposed system consists of four components; RF generator of 370 kHz, copper resonance coupling coils, impedance matching (IM) subsystem and HTS magnet including rectifier system.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.4A
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• pp.407-413
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• 2011

In this paper, the reduced size of broad bandpass filter with parallel coupled line structure is presented. This proposed filter can control bandwidth of narrow to broadband by adjusting the coupling coefficient. The conventional filter is operated with a narrow band. If a higher bandwidth is desired than the conventional narrow bandwidth, it is hard to realize due to the coupling coefficient between feeding line and resonator. In this paper, to overcome this limitation, a proposed bandpass filter is designed with reduced size due to SIR (Stepped Impedance Resonator), U-shaped open stub with hair-pin and vertical-type structure than conventional one, and it has characteristics of adjusting bandwidth freely as per quantity of coupling coefficient. The proposed bandpass filter that, experimental results of insertion and return losses are 0.42 dB and 24.5 dB with bandwidth of 60 % at the center frequency of 5.8 GHz, respectively.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.10 no.7
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• pp.1131-1137
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• 1999

In this paper, a novel design method of an apertured coupled microstrip patch antenna with the single feeding structure is proposed for dual resonance frequencies with mutually perpendicular polarizations. The characteristics of this antenna are experimentally investigated. In order to achieve this goal, a new type of square patch with double notches is used as a radiator and the crossed slot and the bended mictrostrip feeder are adopted for the dual polarizations in the aperture-coupled structure. For the application of the proposed antenna, a Ku-band Tx/Rx $2\times$ subarray antenna is designed and manufactured. Also, the applicability of the antenna as a ground terminal is examined through performance analysis. According to the measurement, the gain of the antenna is 10dBi at the center frequencies of Tx and Rx, the side lobe level is lower than -13dB, and the cross polarization lebel is below 17 dB.