• Bulletin of the Korean Chemical Society
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• v.23 no.4
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• pp.610-612
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• 2002

A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative detection of organophosphorus insecticide cyanophos. An analogue (hapten) of cyanophos was synthesized and was coupled to BSA to produce polyclonal antibodi es from rabbits. The antisera were screened against another hapten coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigen-coated ELISA was developed, which showed an I50 of 310 ng/mL with the detection limit of 20 ng/mL. The antibodies showed negligible cross-reactivities with other organophosphorus pesticides except for parathion-methyl, which makes the assay suitable for the selective detection of cyanophos.

• Water Engineering Research
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• v.2 no.3
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• pp.187-196
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• 2001

An approximate analytical solution of a nonlinear hydro-thermo coupled diffusion equation is derived using the dimensionless form of the equation and transformation method. To derive an analytical solution, it is drastically assumed that the product of first order derivatives in the non-dimensionalized governing equation has little influence on the solution of heat and moisture behavior problem. The validity of this drastic assumption is demonstrated. Some numerical simulation is performed to investigate the applicability of a derived approximate analytical solution. The results show a good agreement between analytical and numerical solutions. The proposed solution may provide a useful tool in the verification process of the numerical models. Also, the solution can be used for the analysis of one-dimensional coupled heat and moisture movements in unsaturated porous media.

• Wind and Structures
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• v.20 no.2
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• pp.275-292
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• 2015

The presence of traffic on a slender long-span bridge deck will modify the cross-section profile of the bridge, which may influence the flutter derivatives and in turn, the critical flutter wind velocity of the bridge. Studies on the influence of vehicles on the flutter derivatives and the critical flutter wind velocity of bridges are rather rare as compared to the investigations on the coupled buffeting vibration of the wind-vehicle-bridge system. A typical streamlined cross-section for long-span bridges is adopted for both experimental and analytical studies. The scaled bridge section model with vehicle models distributed on the bridge deck considering different traffic flow scenarios has been tested in the wind tunnel. The flutter derivatives of the modified bridge cross section have been identified using forced vibration method and the results suggest that the influence of vehicles on the flutter derivatives of the typical streamlined cross-section cannot be ignored. Based on the identified flutter derivatives, the influence of vehicles on the flutter stability of the bridge is investigated. The results show that the effect of vehicles on the flutter wind velocity is obvious.

• Archives of Pharmacal Research
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• v.13 no.1
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• pp.9-13
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• 1990

4, 5-Diphenyl-2-mercaptiomidazole (I) was reacted with hydraziodyl halides IIa-d togive the S-alkyl derivatives III-a-d. Cyclization of IIIa-d afforded imidazo[2, 1-b]-thiazole derivatives Vla, b and VII. Treatment of 1 with a-chloroethylacetoaccetate (IV) gave ethyl 2(4, 5-diphenyl-2-imidazolinylthio)-3-keto-butyrate (V). Compound V coupled with benzendiazonium chloride to give the corresponding phenylhydrazo compound IIId. On heating V with polyphosphoric acid, cyclization took place and 2-acetyl-5, 6-diphenyl-imidazo [2, 1-b] thiazol-3-one (VIII) was obtained. The compound VIII was condensed with aromatic aldehydes to yield the cinnamoyl derivatives 1Xa, b. The antimicrobial activities of compounds IIIa-d, V, VIa, VII were examined.

• Bulletin of the Korean Chemical Society
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• v.24 no.6
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• pp.712-716
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• 2003

The semi-diabatic potential energy surfaces (PESs) of the excited states of polyatomic molecules can be constructed for use in ab initio molecular dynamics (AIMD) studies by relying on the continuity of the electronic energy, oscillator strength, and spherical extent of an excited state along with first derivatives of these quantities as computed by using the equation-of-motion coupled-cluster (EOM-CC) method. The semidiabatic PESs of both the π → $π^*$ valence excited state and the 3s-type Rydberg state of ethylene are presented and discussed in this paper, in conjunction with some of the AIMD results we obtained for these states.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1999.10a
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• pp.359-369
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• 1999

The paper describes the study of concurrent subspace optimization(CSSO) for coupled multidisciplinary design optimization (MDO) techniques in mechanical systems. This method is a solution to large scale coupled multidisciplinary system, wherein the original problem is decomposed into a set of smaller, more tractable subproblems. Key elements in CSSO are consisted of global sensitivity equation(GSE), subspace optimization (SSO), optimum sensitivity analysis(OSA), and coordination optimization problem(COP) so as to inquiry valanced design solutions finally, Automatic differentiation has an ability to provide a robust sensitivity solution, and have shown the numerical numerical effectiveness over finite difference schemes wherein the perturbed step size in design variable is required. The present paper will develop the automatic differentiation based concurrent subspace optimization(AD-CSSO) in MDO. An automatic differentiation tool in FORTRAN(ADIFOR) will be employed to evaluate sensitivities. The use of exact function derivatives in GSE, OSA and COP makes Possible to enhance the numerical accuracy during the iterative design process. The paper discusses how much influence on final optimal design compared with traditional all-in-one approach, finite difference based CSSO and AD-CSSO applying coupled design variables.

• Wind and Structures
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• v.14 no.4
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• pp.359-381
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• 2011

This paper deals with the flutter instability problem of flexible bridge decks in the framework of bimodal-coupled aeroelastic bridge system analysis. Based on the analysis of coefficients of the polynomials deduced from the singularity conditions of an integral wind-structure impedance matrix, a set of simplified formulations for calculating the critical wind velocity and coupled frequency are presented. Several case studies are discussed and comparisons with available approximated approaches are made and presented, along with a conventional complex eigenvalue analysis and numerical results. From the results, it is found that the formulas that are presented in this study are applicable to a variety of bridge cross sections that are not only prone to coupled-mode but also to single-mode-dominated flutter.

• Journal of Ocean Engineering and Technology
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• v.35 no.2
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• pp.99-112
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• 2021

The prediction of maneuverability is very important in the design process of an underwater vehicle. In this study, we predicted the steady turning ability of a symmetrical underwater vehicle while considering interactions between the yaw rate and drift/rudder angle through a simulation-based methodology. First, the hydrodynamic force and moment, including coupled derivatives, were obtained by computational fluid dynamics (CFD) simulations. The feasibility of CFD results were verified by comparing static drift/rudder simulations to vertical planar motion mechanism (VPMM) tests. Turning motion simulations were then performed by solving 2-degree-of-freedom (DOF) equations with CFD data. The turning radius, drift angle, advance, and tactical diameter were calculated. The results show good agreement with sea trial data and the effects on the turning characteristics of coupled interaction terms, especially between the yaw rate and drift angle.

• YAKHAK HOEJI
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• v.36 no.5
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• pp.506-511
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• 1992

The Prostaglandins were derivatized rapidly with monodansyl cadaverine as a fluorophore in mild conditions. The carboxylic moiety of prostaglandins was activated with diethyl phosphorocyanidate and successively coupled with fluorophore in dimethylformamide at room temperature. The labeling yield was reached about 95% at 15 min using arachidic acid $(C_{20:0})$ as a test sample. This derivative showed constant fluorescent intensity at $4^{\circ}C$ for 180 days. The derivatives of prostaglandins were shown high solvent selectivity with tetrahydrofuran in reversed-phase column. therefore, these derivatives could be successfully separated on YMC pack A-212(S-5 120A C8) column in tetrahydrofuran-based eluents. The detection limits of these derivatives was ca. 500 fmol and determination limits was ca. 5 pmol as injected amount in fluorescent detection $({\lambda}ex.\;340\;nm,\;{\lambda}em.\;520\;nm)$. In this method, the ranges of recovery and coefficient of variation were $93.6{\sim}102.7%$ and $4.3{\sim}5.8%$, respectively.

• Journal of Marine Bioscience and Biotechnology
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• v.15 no.2
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• pp.96-102
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• 2023

Salicornia herbacea, a noted halophyte, has been widely researched for its diverse physiological activities. The continuous exploration of its compounds is essential. This study employed gas chromatography (GC) coupled with mass spectrometry (MS) for qualitative analysis. This involved pretreatment including trimethylsilyl (TMS) derivatization of the S. herbacea extract, facilitating its GC analysis. Five compounds, including various fatty acids and β-sitosterol, were identified by direct analysis of the methanol extract of S. herbacea without pretreatment by GC-MS. Conversely, the analysis of the TMS-derivatized extract revealed 28 distinct peaks. Quantitative analysis further indicated that the predominant compounds in the S. herbacea extract were sugars and sugar derivatives, notably glucose, fructose, and glucitol. The collective concentration of these sugars and its derivatives amounted to 116.45 mg/g, representing 11.6% of the dry weight of the raw S. herbacea. Following sugars and their derivatives, fatty acids constituted the next most abundant group of compounds. However, the presence of amino acids and organic acids was relatively low.