YAKHAK HOEJI (약학회지)
- Volume 36 Issue 5
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- Pages.506-511
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- 1992
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
A Sensitive Fluorescent Detection Method for Prostaglandins by High Performance Liquid Chromatography
고속액체크로마토그라피에 의한 프로스타글란딘류의 고감도 형광 분석법
- Lee, Yong-Moon (College of Pharmacy, Chungbuk National University) ;
- Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University)
- Published : 1992.10.29
Abstract
The Prostaglandins were derivatized rapidly with monodansyl cadaverine as a fluorophore in mild conditions. The carboxylic moiety of prostaglandins was activated with diethyl phosphorocyanidate and successively coupled with fluorophore in dimethylformamide at room temperature. The labeling yield was reached about 95% at 15 min using arachidic acid
Keywords
- Prostaglandins;
- monodansyl cadaverine;
- carboxylic moiety;
- fluorescent detection;
- high performance liquid chromatography