• 한국양식학회지
• /
• 제19권4호
• /
• pp.254-260
• /
• 2006

본 연구에서는 국내에서 가장 많이 양식되고 있는 넙치에 있어서 단백질 사료원들의 단백질과 인소화율을 측정을 통해 저오염 사료 개발을 위한 사료원을 선택하는데 그 목적이 있다. 본 실험에 사용된 9가지 동 식물성 단백질원들은 $BAIFA-M^{TM}$(어분대체품), 어분(White fish meal, WFM), 넙치근육분(Flounder muscle powder, FMP), 오징어간분(Squid liver powder, SLP), 수지박(Leather meal, LM), 대두박(Soybean meal, SM), 콘글루텐밀(Corn gluten meal, CGM), 가금부산물(Poultry by-product PBP), 그리고 난단백질(Egg albumin) 이였다. 실험결과, 각 사료원의 단백질 소화율은 FMP, 94%; SLP, 92%; WFM, 86%; SM, 82%; CGM, 75%; LM, 72%; PBP, 72%; $BAIFA-M^{TM}$, 71%; EA, 30%로 나타났다. 각 사료원의 인 소화율은 FMP, 77%; SLP, 72%; $BAIFA-M^{TM}$, 65%; LM, 55%; WFM, 54%; PBP, 50%; CGM, 20%; EA, 20%; SM, 17%로 나타났다.

• Journal of Animal Science and Technology
• /
• 제62권6호
• /
• pp.801-811
• /
• 2020

The main objective of this in vitro study was to evaluate red ginseng byproduct (RGP) as a protein resource and its effects on rumen fermentation characteristics, microflora, CO2, and CH4 production in ruminants. Four treatments for in vitro fermentation using buffered rumen fluid over a 48 h incubation period were used: 1, RGP; 2, corn gluten feed (CGF); 3, wheat gluten (WG); and 4, corn germ meal. In vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), in vitro crude protein digestibility (IVCPD), volatile fatty acids, pH, and ammonia nitrogen (NH3-N) were estimated after 48 h incubation. Gas production was investigated after 3, 6, 12, 24, 36 and 48 h. The CO2 and CH4 were evaluated after 12, 24, 36, and 48 h. A significant difference in total gas production and CO2 emissions was observed (p < 0.01) at all incubation times. CH4 production in RGP were higher (p < 0.05) than that in other treatments but a higher CH4 portion in the total gas production was observed in WG (p < 0.05) at 48 h incubation. The IVDMD, IVNDFD, and IVCPD of RGP was lower than those of other conventional ingredients (p < 0.01). The RGP had the lowest NH3-N value among the treatments (p < 0.01). The RGP also had the lowest total VFA concentration (p < 0.01), but presented the highest acetate proportion and acetate to propionate ratio among the treatments (both, p < 0.01). The abundance of Prevotella ruminicola was higher in RGP than in WG (p < 0.01), whereas RGP has lower methanogenic archaea (p < 0.01). In conclusion, based on the nutritive value, IVDMD, low NH3-N, and decreased methanogenic archaea, RGP inclusion as a protein source in ruminant diets can be an option in replacing conventional feed sources.

• 한국식품위생안전성학회지
• /
• 제37권1호
• /
• pp.15-20
• /
• 2022

국내 유통되는 반려동물 사료의 살모넬라 분석시 증균배양법, 효소면역기법에 의한 분석, 종 특이 primer를 활용한 PCR 방법을 활용하여 비교 평가하였다. 시료 175점 Salmonella spp. 검출 결과 증균배양법 및 종 특이 primer를 활용한 PCR 방법에 의한 검출 방법에서 2점의 시료(육포, 옥수수 글루텐)가 양성으로 확인되었고, 효소면역기법에 의한 검출방법에서는 1점의 시료(옥수수 글루텐)가 양성으로 확인되었다. 증균배양법 및 효소면역기법에 의한 검출방법에 비해 종 특이 primer를 활용한 PCR 방법을 적용 할 경우 시료에서 분리된 균주의 종(species) 판별이 가능하였다.

• Journal of Animal Science and Technology
• /
• 제53권5호
• /
• pp.441-450
• /
• 2011

본 연구는 국내 농산부산물을 이용하여 in vitro 반추위 발효 특성을 조사하고 에너지 사료인 농후사료와 유사한 반추위 발효특성을 나타내는 농산부산물을 선정하여 최적배합수준을 통한 농후사료 대체 효과를 규명하기 위하여 수행하였다. 본 연구를 수행하기 위하여 국내에서 다량 생산되는 9점의 부산물(소맥피, 단백피, 제빵 부산물, 비지, 미강, 청치, 대두피, 주정박, 버섯배지)을 선발하였다. 1차 실험에서는 in vitro 배양을 통해 각각 원료사료의 시간대별(0, 3, 6, 12, 24, 48 h) 반추위 발효특성을 평가하였으며, 옥수수 및 대두박과 유사한 발효특성을 나타내는 원료사료 2종류를 선발 하였다. 최종적으로 선발된 2종류의 원료사료는 다른 원료사료와 혼합한 조합구를 만들어 in vitro 배양을 통한 옥수수와 대두박의 대체효과를 평가하였다. 실험설계는 옥수수와 대두박을 기본배합으로 하여, 알팔파, 티머시 건초 및 옥수수사일리지를 조사료로 사용하였다(T1). T2구는 대두박을 비지로 2.5% 대체, T3구는 옥수수를 청치로 15% 대체하였으며, T4구는 옥수수와 대두박을 청치와 비지로 각각 10 및 5% 대체하여 in vitro 반추위 발효특성을 평가하였다. 각 원료사료와 농산부산물의 in vitro 반추위 발효 특성을 조사한 결과, 옥수수 및 대두박과 유사한 발효특성을 나타내는 농산부산물로 청치와 비지를 선정하였다. 2차 실험에서는 pH의 경우, T4구에서 T1구와 비교하여 유의하게 낮았으며, 가스발생량은 T3 구에서 T1구와 비교하여 유의하게 높았다(p<0.05). 또한 건물 분해율은 3 및 24시간에 T4구에서 T1구와 비교하여 높았다(p<0.05). 결과적으로 농산부산물인 청치와 비지의 사용은 옥수수와 대두박을 일부 대체하기 위해 사용가능할 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제29권8호
• /
• pp.1159-1165
• /
• 2016

The nutritional value of feed proteins and their utilization by livestock are related not only to the chemical composition but also to the structure of feed proteins, but few studies thus far have investigated the relationship between the structure of feed proteins and their solubility as well as digestibility in monogastric animals. To address this question we analyzed soybean meal, fish meal, corn distiller's dried grains with solubles, corn gluten meal, and feather meal by Fourier transform infrared (FTIR) spectroscopy to determine the protein molecular spectral band characteristics for amides I and II as well as ${\alpha}$-helices and ${\beta}$-sheets and their ratios. Protein solubility and in vitro digestibility were measured with the Kjeldahl method using 0.2% KOH solution and the pepsin-pancreatin two-step enzymatic method, respectively. We found that all measured spectral band intensities (height and area) of feed proteins were correlated with their the in vitro digestibility and solubility ($p{\leq}0.003$); moreover, the relatively quantitative amounts of ${\alpha}$-helices, random coils, and ${\alpha}$-helix to ${\beta}$-sheet ratio in protein secondary structures were positively correlated with protein in vitro digestibility and solubility ($p{\leq}0.004$). On the other hand, the percentage of ${\beta}$-sheet structures was negatively correlated with protein in vitro digestibility (p<0.001) and solubility (p = 0.002). These results demonstrate that the molecular structure characteristics of feed proteins are closely related to their in vitro digestibility at 28 h and solubility. Furthermore, the ${\alpha}$-helix-to-${\beta}$-sheet ratio can be used to predict the nutritional value of feed proteins.

• 농업과학연구
• /
• 제44권1호
• /
• pp.86-94
• /
• 2017

In Korea, chemical analysis of animal feed is done through authorized feed analysis laboratories (AFALs). Analysis results among the AFALs need to be similar or within acceptable variations; however, there is no experimental evidence of their comparability. We aimed to determine the level of variation of feed chemical analysis results from different AFALs. For this, we requested analysis of four kinds of feed (corn, soybean meal, corn gluten feed, and ryegrass) to eight AFALs and the Cumberland Valley Analytical Services (CVAS) which is an internationally well-recognized feed analysis laboratory. The AFALs spent more time on analysis than did CVAS. Fiber analysis results varied significantly among laboratories. However, moisture, CP, and ash content values showed almost no variation. At least one AFAL obtained results with significant differences from CVAS for all tested values. These differences can be explained by the followings: 1) the standard methods for feed analysis (SMFA) established for AFALs are not detailed enough to control the analytical variations among different laboratories and 2) guidelines are insufficient for the quality control of analysis results in Korea. Failure to accurately identify the nutritional components of the feed could mean failure to provide adequate nutrients to the animals. Therefore, efforts to reduce the differences among AFALs, such as revising SMFA and publishing guidelines on quality control of feed analysis results, are important.

• 한국식품과학회지
• /
• 제18권5호
• /
• pp.357-363
• /
• 1986

식품의 주요 구성 성분의 현탁액에 대한 열전도도와 열확산도를 $-40^{\circ}C\;-150^{\circ}C$의 온도 범위와 10%-60%의 농도 범위애서 측정하였다. 세 종류의 서로 다른 농도에서 측정된 실험값으로부터 가정된 모델에 의해 순수 성분의 열전도도와 열확산도를 각각의 온도에서 구한 다음 컴퓨터 프로그램을 이용하여 모델의 상수들을 구하였다. 본 연구에서 발전시킨 모델을 사용하여 얻은 식품의 열전도도와 열확산도 수치를 기발표된 모든 액체식품의 데이타와 비교해 본 결과 3.8%의 오차가 있었다. 식품의 주요 구성 성분의 함량과 온도가 주어지면 본 연구에서 발전시킨 모델을 이용하여 열전도도와 열확산도 수치를 위의 오차 한계에서 구할 수 있다.

• 한국미생물·생명공학회지
• /
• 제19권6호
• /
• pp.614-618
• /
• 1991

토양세균 B.subtilis subsp. krictiensis로부터 항진균활성물질 KRF-001 복합체를 생산하기 위한 발효 조건을 조사하였다. Whey와 mannitol 등이 탄소원으로, CSL, corn gluten meal 그리고 polypeptone이 복합질소원으로 좋았다. 중온균인 생산균은 중성 pH조건에서 P.oryzae에 대한 bioactivity가 가장 높았으며, 0-10 DO의 낮은 용존산소 조건에서도 bioactivity가 유지되었다. 인산염의 농도가$ K_2HPO_4$, 0.047에서 0.097로 높아짐에 따라 1/2의 bioactivity 감소가 관찰되었다. 한편 UV 및 MNNG에 의한 돌연변이에 의해 우수균주 3개를 선별하였다.

• Journal of Microbiology and Biotechnology
• /
• 제21권8호
• /
• pp.869-873
• /
• 2011

Most medium formulations for improving culture of entomopathogenic nematodes (EPN) based on protein sources have used enriched media like animal feed such as dried egg yolk, lactalbumin, and liver extract, among other ingredients. Most results, however, showed unstable yields and longer production time. Many of the results do not show the detailed parameters of fermentation. Soy flour, cotton seed flour, corn gluten meal, casein powder, soytone, peptone, casein hydrolysates, and lactalbumin hydrolysate as protein sources were tested to determine the source to support optimal symbiotic bacteria and nematode growth. The protein hydrolysates selected did not improve bacterial cell mass compared with the yeast extract control, but soy flour was the best, showing 75.1% recovery and producing more bacterial cell number ($1.4{\times}10^9$/ml) than all other sources. The highest yield ($1.85{\times}10^5$ IJs/ml), yield coefficient ($1.67{\times}10^6$ IJs/g medium), and productivity ($1.32{\times}10^7$ IJs/l/day) were also achieved at enriched medium with soybean protein.

• 한국수산과학회지
• /
• 제49권2호
• /
• pp.146-153
• /
• 2016

A feeding experiment was conducted to determine the nutritional value of various dietary protein sources without a vitamin premix supplementation in juvenile black rockfish, Sebastes schlegeli. Diets containing similar quantities of lipid and carbohydrate with 50% crude protein from fish meal (FM)/soybean meal (SBM)/corn gluten meal (CGM), FM only, casein/gelatin, high FM/low casein and low FM/high casein were fed to rockfish along with a commercial rockfish diet. The diet containing only 65.6% FM and the commercial rockfish diet produced significantly greater weight gain (WG) and feed efficiency (FE) than all other semipurified diets (SPDs); the economical diet with 52.8% FM, 10% SBM and 5% CGM produced lower WG than the diet containing FM and commercial rockfish diet, but FE and survival were similar both diets. Intermediate responses were observed for rockfish fed the SPD containing 32.8% FM and 27.4% casein, while SPDs containing 10% FM and 41.8% casein and 38.5% casein and 10% gelatin yielded the lowest WG and FE. Some differences in tissue indices and body composition of rockfish were induced by the diets containing various protein sources and levels without the vitamin premix supplementation.