• Journal of Life Science
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• v.31 no.3
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• pp.257-265
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• 2021

Heterotrimeric kinesin-2 is a molecular motor protein of the kinesin superfamily (KIF) that moves along a microtubule plus-end directed motor protein. It consists of three different motor subunits (KIF3A, KIF3B, and KIF3C) and a kinesin-associated protein 3 (KAP3) that form a heterotrimeric complex. Heterotrimeric kinesin-2 interacts with many different binding proteins through the cargo-binding domain of the KIF3s. The activity of heterotrimeric kinesin-2 is regulated to ensure that the cargo is directed to the right place at the right time. How this regulation occurs, however, remains in question. To identify the regulatory proteins for heterotrimeric kinesin-2, we performed yeast two-hybrid screening and found a specific interaction with creatine kinase B (CKB), which is the brain isoform of cytosolic creatine kinase enzyme. CKB bound to the cargo-binding domain of KIF3A but did not interact with the KIF3B, KIF5B, or KAP3 in the yeast two-hybrid assay. The carboxyl (C)-terminal region of CKB is essential for the interaction with KIF3A. Another protein kinase, CaMKIIa, interacted with KIF3A, but GSK3a did not interact with KIF3A in the yeast two-hybrid assay. KIF3A interacted with GST-CKB-C but not with GSK-CKB-N or GST alone. When co-expressed in HEK-293T cells, CKB co-localized with KIF3A and co-immunoprecipitated with KIF3A and KIF3B but not KIF5B. These results suggest that the CKB-KIF3A interaction may regulate the cargo transport of heterotrimeric kinesin-2 under energy-compromised conditions in cells.

• Journal of Life Science
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• v.34 no.5
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• pp.333-338
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• 2024

Kinesin-1 is a heterotetrameric protein composed of two heavy chains (KHCs, also known as KIF5s) with a motor domain and two light chains (KLCs) without a motor domain. KIF5 has three subtypes, namely, KIF5A, KIF5B, and KIF5C, which share high amino acid homology except in their carboxy (C)-terminal region. KIF5A is responsible for transporting cargo within the cell. The adaptor proteins that bind to the C-terminal region of KIF5A mediate between kinesin-1 and cargo. However, the proteins regulating the intracellular cargo transport of kinesin-1 have not yet been fully identified. In this study, we identified ADP-ribosylation factor GTPase-activating protein 1 (ArfGAP1), which is involved in the intracellular trafficking of lysosomes, as a binding partner of KIF5A. KIF5A binds to the C-terminal region of ArfGAP1, and ArfGAP1 binds to the C-terminal region of KIF5A but does not interact with KIF5B, KIF5C, kinesin light chain 1 (KLC1), or KIF3A. When co-expressed in mammalian cells, ArfGAP1 co-localized with KIF5A and co-immunoprecipitated with KIF5A, KIF5B, and KLC1, but not with KIF3B. These results suggest that kinesin-1 may be regulated by ArfGAP1 in the intracellular transport of cargo.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.47 no.4
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• pp.79-89
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• 2010

Recently, distributed video coding (DVC) has been actively studied for low complexity video encoder. The complexity of the encoder in DVC is much simpler than that of traditional video coding schemes such as H.264/AVC, but the complexity of the decoder in DVC increases. In this paper, we propose the Region-Of-Interest (ROI) based DVC with low decoding complexity. The proposed scheme uses the ROI, the region the motion of objects is quickly moving as the input of the Wyner-Ziv (WZ) encoder instead of the whole WZ frame. In this case, the complexity of encoder and decoder is reduced, and the bite rate decreases. Experimental results show that the proposed scheme obtain 0.95 dB as the maximum PSNR gain in Hall Monitor sequence and 1.87 dB in Salesman sequence. Moreover, the complexity of encoder and decoder in the proposed scheme is significantly reduced by 73.7% and 63.3% over the traditional DVC scheme, respectively. In addition, we employ the layered belief propagation (LBP) algorithm whose decoding convergence speed is 1.73 times faster than belief propagation algorithm as the Low-Density Parity-Check (LDPC) decoder for low decoding complexity.

• Journal of Digital Convergence
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• v.10 no.6
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• pp.133-140
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• 2012

This study starts from interests on an endeavor of each economy unit, especially the enterprises, for the survival and sustainability of themselves. Competition, how to get it, maintain it and advance it! Agreeing the perceivedness of industries, scholars and public; what the core competition of manufacturer is an competitiveness of product, this study focuses on the PLM system as the key solution for obtaining the competitiveness of product with reviewing the contents and studies of PLM formulated. PLM is highlighted as the one of top tier ICT solution for the product development domain of manufacturing industry today. First of all, this study deals with the opinion of actual work-site and management of our small&medium business for the benefits of PLM based on product development; the impact on the performances of management, which is the key objective of the existence of enterprises. In conclusion, the answers for these questionnaires shows positive perception of actual work-sites and managers for the PLM system. They agree some useful effects on shortening the term and downsizing the cost of product development due to Data integration and Process management, even if they have not full confidence on the effectiveness of the individual functionality of the PLM system.

• Journal of the Korean Society of Groundwater Environment
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• v.6 no.1
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• pp.23-32
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• 1999

A new numerical method using solution-adaptive grids (SAG) is developed to solve the Richards' equation (RE) for unsaturated flow in porous media. Using a grid generation technique, the SAG method automatically redistributes a fixed number of grid points during the flow process, so that more grid points are clustered in regions of large solution gradients. The method uses the coordinate transformation technique to employ a new transformed RE, which is solved with the standard finite difference method. The movement of grid points is incorporated into the transformed RE, and therefore all computation is performed on fixed grid points of the transformed domain without using any interpolation techniques. Thus, numerical difficulties arising from the movement of the wetting front during the infiltration process have been substantially overcome by the new method. Numerical experiments for an one-dimensional infiltration problem are presented to compare the SAG method to the modified Picard method using a fixed grid. Results show that accuracy of a SAG solution using 41 nodes is comparable with the solution of the fixed grid method using 201 nodes, while it requires only 50% of the CPU time. The global mass balance and the convergence of SAG solutions are strongly affected by the time step size (Δt) and the weighting parameter (${\gamma}$) used for generating solution-adaptive grids. Thus, the method requires automated readjustment of Δt and ${\gamma}$ to yield mass-conservative and convergent solutions, although it may increase computational costs. The method can be effective especially for simulating unsaturated flow and other transport problems involving the propagation of a sharp-front.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.728-739
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• 2020

Using webometric big data, this study examines the diversity of online information sources about the novel coronavirus causing the COVID-19 pandemic. Specifically, it focuses on some 28 countries where confirmed coronavirus cases occurred in February 2020. In the results, the online visibility of Australia, Canada, and Italy was the highest, based on their producing the most relevant information. There was a statistically significant correlation between the hit counts per country and the frequency of visiting the domains that act as information channels. Interestingly, Japan, China, and Singapore, which had a large number of confirmed cases at that time, were providing web data related to the novel coronavirus. Online sources were classified using an N-tuple helix model. The results showed that government agencies were the largest supplier of coronavirus information in cyberspace. Furthermore, the two-mode network technique revealed that media companies, university hospitals, and public healthcare centers had taken a positive attitude towards online circulation of coronavirus research and epidemic prevention information. However, semantic network analysis showed that health, school, home, and public had high centrality values. This means that people were concerned not only about personal prevention rules caused by the coronavirus outbreak, but also about response plans caused by life inconveniences and operational obstacles.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.38 no.2
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• pp.110-118
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• 2002

The element free Galerkin method has been suggested by Belyschko, etc. it is applied for linear elastic analysis of solid problems and its convergence characteristics and stability are shown according to the weight function, influence domain and scattered points. The various numerical examples are performed to check the efficiency of 1D EFG and 2D EFG program by changing factors. As a result it have the best results when it used the cubic spline weight function and the scaling parameter d$_{max}$=2. These programs were developed by mixed language programming method using Visual Basic and the C language. so it is fast and efficient. and visually shown the result.t.

• Journal of Digital Convergence
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• v.12 no.7
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• pp.133-145
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• 2014

Recently, Hospital information systems have the large databases by wide range offices for hospital management, health care to improve the quality of care. However, hospital information systems for information security measures are insufficient. Therefore, when we construct the hospital information system, we have to audit the information security measures for them, and we have to manage the ISMS(Information Security Management System) to maintain the information protection level through the risk managements. In this paper, we suggested the hospital information security audit model for the protection of health information privacy by the current hospital information systems, information security management system(ISMS), and hospital information security requirements and threats. We derived the check items compared with ISO27799 reflected the characteristics of the hospital. We classified the security domains as the physical, technical, administrative domain, and derived the check items for information security. We also designed the check lists by mapping the ISO27799 risk management process to improve the security and efficiency simultaneously. Our model by the five-point scale survey of IT experts was verified the suitability with the average of 4.91 points.

• Journal of Digital Convergence
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• v.18 no.11
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• pp.215-226
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• 2020

Chatbot is emerging as an important interface window for business. This change is due to the continued development of chatbot-related research from NLP to NLU and NLG. However, the reality is that the methodological study of drawing domain knowledge and developing it into a user-friendly interactive interface is weak in the process of developing chatbot. In this paper, in order to present the process criteria of chatbot development, we applied it to the actual project based on the methodology presented in the previous paper and improved the development methodology. In conclusion, the productivity of the test phase, which is the most important step, was improved by 33.3%, and the number of iterations was reduced to 37.5%. Based on these results, the "3 Phase and 17 Tasks Development Methodology" was presented, which is expected to dramatically improve the trial and error of the chatbot development.

• Journal of Digital Convergence
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• v.15 no.8
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• pp.455-463
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• 2017

Background/Objectives: The purpose of the study is to derive a goal usable in the field by exploring the case management success experiences of case managers. Civil case managers state that what is still important in case management practice is qualitative change made in the practice process. Therefore, the study will deal with detailed contents about case management that civil domain case managers consider to be important. Methods/Statistical analysis: For data collection, the study used focus group interview on 20 professional case management social workers and used Grounded theory method by Glaser for data analysis. The analysis of collected data consisted of a process of finding the categories and characteristics of the research problem. Findings: As a result of analysis, 4 categories, 10 subcategories, and 25 elements were derived for success experiences of case management practice. The categories were divided into satisfaction and ability improvement of client, case manager ability improvement, establishment of organization and environment function, and multilateral trust relationship strengthening. Improvements/Applications: The results of this study further clarified the goal of field case management practice and it was suggested as a plan to reflect on future case management related institutions evaluation.