• International Journal of Oral Biology
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• 제45권4호
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• pp.197-203
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• 2020

Mesenchymal stem cells in the dental pulp exhibit a tendency for differentiation into various dental lineages and hold great potential as a major conduit for regenerative treatment in dentistry. Although they can be readily isolated from teeth, the exact characteristics of these stem cells have not been fully understood so far. When compared to two-dimensional (2D) cultures, three-dimensional (3D) cultures have the advantage of enriching the stem cell population. Hence, 3D-organoid culture and 3D-sphere culture were applied to dental pulp cells in the current study. Although the establishment of the organoid culture proved unsuccessful, the 3D-sphere culture readily initiated the stable generation of cell aggregates, which continued to grow and could be passaged to the second round. Interestingly, a significant increase in SOX2 expression was detected in the 3D-spheroid culture compared to the 2D culture. These results indicate the enrichment of the stemness-high population in the 3D-sphere culture. Thus, 3D-sphere culture may act as a link between the conventional and 3D-organoid cultures and aid in understanding the characteristics of dental pulp stem cells.

• 한국산림과학회지
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• 제97권6호
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• pp.650-655
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• 2008

유칼리나무 펠리타(Eucalyptus pellita) 선발목(5년생)의 기내 대량증식을 위해 1L 규모로 1) 고체배양(대조구), 2) 액체정치배양, 3) 개방형 액체배양 등 3가지 배양법이 시도 되었고, 그 결과를 토대로 배양규모를 10L로 확대하여 대량배양 가능성을 조사하였다. 1L 배양용기에서 4주간 배양한 결과, 공기가 공급 된 개방형 액체배양에서 가장 좋은 생장 결과를 얻었다. 이는 액체배지에 의한 원활한 염류 공급과 배양용기내 공기의 환기에 의해 얻어진 결과로 생각된다. 배양 규모를 10L로 확대하여 액체대량배양을 실시한 결과, 식물체의 생장은 대조구인 고체배양에 비교하여 마디 수에 있어서 370%, 엽 면적 3.6배, 그리고 신초 길이 3.3배 신장하는 결과를 보였다. 이는 개방형 액체대량배양 시스템이 유칼리나무 클론증식시 생산성을 향상시키는데 적합한 시스템임을 보여주는 결과라고 하겠다.

• KSBB Journal
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• 제26권1호
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• pp.87-91
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• 2011

Automatic counting of yeast cells in baker's yeast culture was tried using a conventional light microscope equipped with a pc camera. Relatively good binary image was obtained by using white LED as microscope light source, but uneven brightness distribution in original image hindered counting accuracy. A block binarization method using local thresholds proportional to local brightnesses was used to get improved binary images. The brightnesses of the blocks were expressed as the value component in HSV color model. Good quality binary images were obtained by binarization on $8{\times}6$ blocks of original images and connected-component labelling of the binarized images produced reliable counting results in the concentration range $1.4{\times}10^5/mL{\sim}1.4{\times}10^7\;cells/mL$.

• International Journal of Advanced Culture Technology
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• 제4권4호
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• pp.23-29
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• 2016

In this paper, the learning technique for CNN processor on vehicle is proposed. In the case of conventional CNN processors, weighted values learned through training are stored for use, but when there is distortion in the image due to the weather conditions, the accuracy is decreased. Therefore, the method of enhancing the input image for classification is general, but it has the weakness of increasing the processor size. To solve this problem, the CNN performance was improved in this paper through the learning method of the distorted image. As a result, the proposed method showed improvement of approximately 38% better accuracy than the conventional method.

• International Journal of Advanced Culture Technology
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• 제9권3호
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• pp.283-290
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• 2021

Recently, correlated superposition coding (CSC) has been proposed to implement non-orthogonal multiple access (NOMA) without successive interference cancellation (SIC), without loss of spectral efficiency, in contrast to conventional independent superposition coding (ISC). However, correlation between signals has reduced the average total allocated power, which results in degraded performance. Thus, in order to avoid the reduction of the average total allocated power owing to correlation between signals, this paper proposes a cross-correlated quadrature amplitude modulation (QAM) NOMA scheme under Rayleigh fading channel surroundings. First, we design the cross-correlated QAM NOMA scheme. Then, simulations demonstrate that for the weaker channel gain's user, the symbol error rate (SER) performance of the proposed cross-correlated QAM NOMA improves largely, whereas for the stronger channel gain's user, the SER performance of the proposed cross-correlated QAM CSM NOMA degrades little, compared to that of the conventional QAM NOMA.

• International Journal of Advanced Culture Technology
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• 제9권4호
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• pp.307-314
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• 2021

Recently, positively asymmetric binary pulse amplitude modulation (2PAM) has been proposed to improve the bit error rate (BER) performance of the weak channel gain user, with a tolerable BER loss of the strong channel gain user, for non-orthogonal multiple access (NOMA). However, the BER loss of the stronger channel gain user is inevitable in such positively asymmetric 2PAM NOMA scheme. Thus, we propose the negatively asymmetric 2PAM NOMA scheme. First, we derive closed-form expressions for the BERs of the negatively asymmetric 2PAM NOMA. Then, simulations demonstrate that for the stronger channel gain user, the BER of the proposed negatively asymmetric 2PAM NOMA improves, compared to that of the conventional positively asymmetric 2PAM NOMA. Moreover, we also show that for the weaker channel gain user, the BER of the proposed negatively asymmetric 2PAM NOMA is comparable to that of the conventional positively asymmetric 2PAM NOMA, over the power allocation range less than about 10 %.

• 한국의류학회지
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• 제24권4호
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• pp.538-548
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• 2000

A study analyzes the eclectic trend shown in Andy Warhol look to explain the pluralistic aspect which is expressed in the late 20th century's fashion. Eclecticism is a trial to dismantle the barriers between pure art and popular art, high culture and low culture. This pursues harmonious accommodation and coexistence of all areas without being confined in the conventional lofty taste or high art. This thesis studies the characteristic aspects of the eclectic trend by classifying this trend into sell culture, mass media and fashion around the Andy Warhol look. The sexual eclectic trend in Warhol look is bisexual one shown in clubland and the world of modeling and supplies fashion with abundant expression and ambiguity. Warhol established cultural eclecticism by compounding high cultural factors and low cultural factors. broke up the boundary between street fashion and high fashion and escaped from the fixed idea on materials and design. Also he generalized and democratized specialty or nobility conventional paintings had possessed through introducing repetition and mediocrity and fully utilized every kind of mass media, Hollywood movie stars and daily necessaries in producing works. Andy Warhol who asserted‘Business Art’that was the mixed form of artistry and commercialism had creative and futuristic taste and proposed the direction to develop current fashion and art where the concept of economy is importantly brought into relief.

• 한국환경농학회지
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• 제19권5호
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• pp.433-441
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• 2000

A feasibility study was performed to examine the agronomic application of treated sewage on paddy rice culture by field experiment for two consecutive years. The domestic sewage was treated by the constructed wetland system which was in subsurface flow type and consisted of sand and macrophyte. The effluent of the wetland system was used for irrigation water. The effluent was diluted to maintain the total nitrogen concentration below $26mg{\cdot}L^{-1}$ in the first year and used without dilution in the second year experiment. Growth components and yields were compared against the CONTROL plot where conventional method was applied. And also, soil characteristics of the plots before and after reclaimed sewage irrigation were analyzed. Generally, addition of the treated sewage to the irrigation water showed no adverse effects on paddy rice culture, and even enhancement was noticed in both growth and yield. Irrigation of treated sewage after concentration adjusted with conventional fertilization showed the better result, and the yield exceeded that of CONTROL case where clean water was irrigated. Soil characteristics changed after irrigation, and significant EC increasing was observed for the reclaimed sewage irrigation plots. From this study, it appears that reuse of treated sewage, as supplemental irrigation water could be a feasible and practical alternative. For full-scale application, further study is recommended on the specific guideline of major water quality components in treated sewage for irrigation and public health.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1928-1935
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• 2015

Microbial growth kinetics is often used to optimize environmental processes owing to its relation to the breakdown of substrate (contaminants). However, the quantification of bacterial populations in the environment is difficult owing to the challenges of monitoring a specific bacterial population within a diverse microbial community. Conventional methods are unable to detect and quantify the growth of individual strains separately in the mixed culture reactor. This work describes a novel quantitative PCR (qPCR)-based genomic approach to quantify each species in mixed culture and interpret its growth kinetics in the mixed system. Batch experiments were performed for both single and dual cultures of Pseudomonas putida and Escherichia coli K12 to obtain Monod kinetic parameters (μ_max and K_s). The growth curves and kinetics obtained by conventional methods (i.e., dry weight measurement and absorbance reading) were compared with that obtained by qPCR assay. We anticipate that the adoption of this qPCR-based genomic assay can contribute significantly to traditional microbial kinetics, modeling practice, and the operation of bioreactors, where handling of complex mixed cultures is required.

• Clinical and Experimental Reproductive Medicine
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• 제41권2호
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• pp.41-46
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• 2014

IVM refers to the maturation of immature oocytes in culture after their recovery from small antral follicles at the stage prior to selection and dominance. IVM requires little or no FSH in vivo and has been proposed as an alternative to conventional IVF, since it reduces the primary adverse effects caused by controlled ovarian stimulation, including the ovarian hyperstimulation syndrome. Moreover, IVM is a promising option for cases for which no standard protocol is suitable, such as FSH resistance, contraindications for ovarian stimulatory drugs, and the need for urgent fertility preservation. Recently, IVM has been used in women with regular cycles and normal ovaries. However, the pregnancy rate following IVM is suboptimal compared with that of conventional IVF, indicating that further studies to optimize the protocol and the culture conditions are warranted.