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Application of Open-type Liquid Culture for Large-scale Production of Mature Plus Tree of Eucalyptus pellita  

Park, So-Young (Biotechnology Division, Korea Forest Research Institute)
Moon, Heung-Kyu (Biotechnology Division, Korea Forest Research Institute)
Kim, Yong-Wook (Biotechnology Division, Korea Forest Research Institute)
Kim, Seon-Ja (Biotechnology Division, Korea Forest Research Institute)
Yi, Jae-Seon (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
Publication Information
Journal of Korean Society of Forest Science / v.97, no.6, 2008 , pp. 650-655 More about this Journal
Abstract
In an attempt to establish the mass proliferation system, Eucalyptus pellita, a 5-year-old plus tree, was cultured with three different culture types in 1L vessels: solid culture without ventilation (conventional culture), liquid culture without ventilation and open-type liquid culture with forced ventilation. Then the culture scale was subsequently increased from 1L to 10L in vessel volume. After 4 weeks of 1L-scale culture, the best growth was obtained by culturing plantlets on open-type liquid culture, suggesting that the in vitro plantlets growth can be enhanced by liquid medium and ventilation. In open-type large scale culture in 10L vessel, plantlets growth resulted in a 370% increase in the number of nodes, 3.6 times increase in leaf expansion, and 3.3 times increase in shoot length, while the conventional culture suppressed shoot growth due to the callusing on the leaves and lack of $CO_2$. The results indicated that the open-type large scale culture system was effective for enhancing productivity by improving growth of the plantlets in clonally proliferated plus tree, Eucalyptus pellita.
Keywords
Eucalyptus pellita; large scale culture; liquid medium; clonal propagation;
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