KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Automatic Counting of Yeast Cells in Baker's Yeast Culture Using PC Camera and Conventional Light Microscope

PC카메라와 일반광학현미경을 이용한 빵효모 배양액의 효모세포 자동계수

  • Received : 2010.08.05
  • Accepted : 2011.01.22
  • Published : 2011.02.28
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Abstract

Automatic counting of yeast cells in baker's yeast culture was tried using a conventional light microscope equipped with a pc camera. Relatively good binary image was obtained by using white LED as microscope light source, but uneven brightness distribution in original image hindered counting accuracy. A block binarization method using local thresholds proportional to local brightnesses was used to get improved binary images. The brightnesses of the blocks were expressed as the value component in HSV color model. Good quality binary images were obtained by binarization on $8{\times}6$ blocks of original images and connected-component labelling of the binarized images produced reliable counting results in the concentration range $1.4{\times}10^5/mL{\sim}1.4{\times}10^7\;cells/mL$.

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References

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