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The Characteristics of Toxin Production in the Korean Toxic Cyanobacteria (국내산 유독 남조류의 독소생산 특성)

  • Kim, Hwa-Bin;Park, Hae-Kyung;Shin, Kyodong;Moon, Jeong-Suk
    • Journal of Korean Society on Water Environment
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    • v.26 no.5
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    • pp.834-840
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    • 2010
  • To find out the toxin production characteristics of Korean harmful cyanobacteria, we isolated 14 cyanobacterial strains from Korean lakes and rivers and analyzed the kinds and cellular content of microcystins (MCYSTs) of cyanobacterial isolates using cultured biomass. And we measured the MCYSTs production by growth phase of two representative toxic strains, Microcystis aeruginosa (HG-015) and Anabaena planktonica (HG-012). Among seven cyanobacteral species, Microcystis wesenbergii showed the highest cellular MCYSTs content. MCYST-RR was the most dominant toxin reaching more than 85% of MCYSTs produced by isolated cyanbacterial strains. During the mass culture, Microcystis aeruginosa (HG-015) showed the highest yield and accumulation of MCYSTs in the exponential growth phase. However the cellular content of chlorophyll a and MCYSTs of Anabaena planktonica (HG-012) showed higher value in the stationary and early death phase than in the exponential growth phase. Our results suggest that control and removal of harmful cyanobacterial bloom before exponential growth phase may be effective to prevent health risk of cyanobacterial toxins in the drinking water sources.

Stratification of Lipid Content and Composition in Blubber of Marine Cetacean from Korean Waters (한국 근해에 서식하는 고래 피하지방의 층별 지방 함량 및 구성 변화)

  • Ko, Ah-Ra;Ju, Se-Jong;Moon, Dae-Yeon;Choi, Seok-Gwan;Kim, Zang-Geun;Shin, Kyung-Hoon
    • Ocean and Polar Research
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    • v.33 no.1
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    • pp.35-43
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    • 2011
  • Blubber plays different biological roles in cetaceans, including structural support, physiological demands (thermoregulation, streamlining, buoyancy, etc.), and energy storage. As such, biochemical composition, especially lipid, in the blubber may be vertically stratified in order to effectively assist with these functions. We analysed lipid content, class and fatty acid composition in the blubber of by-catch minke whale (Balaenoptera acutorostrata; n = 11), long-beaked common dolphin (Delphinus capensis; n = 3) and Pacific white-sided dolphin (Lagenorhynchus obiquidens; n = 2) from Korean waters. Lipid content for all three species ranged from 55 to 96% of wet weight, with a dominance of triacylglycerols. Vertical stratification of blubber lipid content was different between species. For minke whale and longbeaked common dolphin, it increased from inner (near the muscle) to outer (near the skin) layer, whereas it decreased for Pacific white-sided dolphin. Thirty-one fatty acids were found in all blubber samples and vertical stratification of fatty acid composition was similar among all three species. Among grouped fatty acids, monounsaturates were the most abundant, gradually increasing from inner to outer blubber layer, whereas polyunsaturates and saturates decreased. Such vertical stratification of lipid content and composition suggests that the modification and accumulation of lipid occur in the blubber itself in order to help cetaceans with their specific physiological needs.

Nutritional Components and Physiological Activity of Centella asiatica Cultured in Chungju by Drying Method (건조방법에 따른 충주산 병풀의 영양성분과 생리활성)

  • Eom, Hyun-Ju;Shin, Hyun-Young;Ji, Yeong Mi;Kwon, Nu Ri;Yoon, Hyang-Sik;Kim, In Jae;Song, Yong-sup;Yu, Kwang-Won
    • The Korean Journal of Food And Nutrition
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    • v.34 no.2
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    • pp.165-173
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    • 2021
  • Centella asiatica (CA) has been used as a nutritional plant as well as a traditional herbal medicine around the world. This study, quality component (proximate composition, total polyphenol, and triterpenoid compound), and antioxidant and anti-inflammatory activities of CA dried using various methods were investigated. Proximate compositions of CA with different drying methods included a large amount of carbohydrates, crude protein, crude ash, and crude fiber. Among the drying methods, cold drying provided the highest total polyphenol content and antioxidant activity, while hot-air drying at 75℃ provided the lowest total polyphenol content and antioxidant activity. In addition, when the major triterpenoid compounds of CA were analyzed, the highest content of asiaticoside of triterpenoid glycoside was obtained with all drying methods. With respect to the total triterpenoid, the highest content was obtained with cold drying (68.8 mg/g) whereas natural drying (31.4 mg/g) provided the lowest content. In anti-inflammatory activity of LPS-stimulated RAW 264.7 cells, EtOH extract of cold drying showed a significantly higher inhibitory activity in comparison to the other drying methods. In conclusion, it is considered that the cold drying method is suitable for industrial preparation of functional materials with high physiological ingredients, and antioxidant and anti-inflammatory activities from CA.

Application of Rapid Sample Preparation Method and Monitoring for Cholesterol Content in Chicken Egg and Egg powder

  • Park, Jung-Min;Jeong, In-Seek;Kwak, Byung-Man;Ahn, Jang-Hyuk;Leem, Donggil;Jeong, Jayoung;Kim, Jin-Man
    • Food Science of Animal Resources
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    • v.33 no.5
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    • pp.672-677
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    • 2013
  • The aim of this study was to develop sample preparation method and evaluate the exact cholesterol content in egg and egg powder purchased from Korean markets, and to determine whether significant differences exist among various egg products, since a variety of products are available in Korean markets and there are no recent databases for cholesterol. To evaluate the cholesterol content in chicken egg sold in Korean local market, a simple method using non-heated saponification to determine cholesterol for emulsified foods was applied. The results of recovery for egg and egg powder were in a range of 92.4-105.0%, with a relative standard deviation between 1.1% and 2.8% by using gas chromatography-flame ionization detector. Therefore, the total cholesterol content in whole egg was estimated between 160.8 and 226.3 mg/egg (AV(average) $186.8{\pm}3.5$), which is similar or lower than previously reported levels. The value for cholesterol in egg powder was estimated between 2.94 and 3.49 mg/g (AV $3.23{\pm}0.15$). We suggested method that can be applicable to chicken egg and egg powder matrix as providing rapid and accurate determination of cholesterol in egg and egg powder. This information will be helpful for processed food producers for deciding food labels of cholesterol content.

Study on Wine Quality of Domestic Grape Cultivar 'Cheonghyang' classified by Ripening Stage (국내 육성 품종 '청향' 포도의 숙도별 양조 적성 연구)

  • Lee, Hyo-Young;Jeon, Jin-A;Park, Young-Sik;Yi, Jae-Hyoung;Kwon, Hye-Jeong;Jeong, Seok-Tae
    • Journal of the East Asian Society of Dietary Life
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    • v.26 no.5
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    • pp.445-449
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    • 2016
  • In this study, Red pearl (4x) and MBA (2x) were crossed Gangwon-do Agricultural Research and Extension Services in 2000 to compare the brewing characteristics of Cheonghyang, a seedless kind of grape registered in 2009, by maturity. Fruit characteristics of Cheonghyang were examined after the first racking and 300 days after fermentation during aging in manufacturing wine based on maturity categories of being immature, mature, and overmature after harvest between August 25 and 27, 2014. The soluble solid matter content did not differ between the first racking following manufacturing wine and during ripening, and the alcohol content following the first racking was 12.9% for immature ones, 13.0% for mature ones, and 12.7% for overmature ones., The alcohol content decreased by appropriately 1.3% to 1.8% during ripening. The total acidity content was 0.9% for immature ones, 0.8% for mature ones, and 0.5% for overmature ones following the first racking and showed insignificant variations during ripening of 0.8%, 0.7%, and 0.5%, respectively, with wine made from mature ones showing proper acidity. The volatile acid content following the first racking during ripening, showing insignificant variation by maturity; however, it decreased after ripening. The sensory evaluation detected differences in acidity of wine between mature and overmature grapes. There was no difference in sour taste between immature and mature grapes; however, overmature ones had low levels of sour taste. Our results showed that mature wine had the best overall preference.

A Study on the Interest of SNS Users according to New Media Fashion Content Types -Focus on Vogue Korea's Official Instagram- (뉴미디어 패션 콘텐츠 유형에 따른 사용자의 SNS 관심도 연구 -보그 코리아 공식 인스타그램 중심으로-)

  • Lee, Chungsun;Lee, Seunghee
    • Journal of Fashion Business
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    • v.24 no.1
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    • pp.75-87
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    • 2020
  • The purpose of this study is to find trends in new media fashion content by analyzing the fashion content of the official Instagram accounts of domestic fashion magazines that are being transformed by digital media. The framework for these analysis of fashion content type and methods of production is based on one used in an earlier research project. Empirical analysis is conducted on Vogue Korea's official Instagram accounts, using the highest number of major views as the secondary measure of interest. After screening for fashion content in posts on the Vogue Korea account for four months, 291 short video postings were extracted to analyze the number of views the postings received. The results were categorized as 'star', 'show/exhibition', 'product', 'shop', 'fashion film', 'designer', or 'event', included in the data are the number of postings by type and the number of views by post. Based on the characteristics of the creator and the editing, the posts were classified into 'professional production highlight', 'professional production private', 'UCC' or 'GIF' videos, the number of views per post were also collected. The research results show different levels of interest depending on the type of fashion content, and also on the way the videos were produced. The study also investigated how the combination of these two factors affects interest. When producing a new media fashion content, combining a 'star' type post with 'professional production private' video content was most popular. The selection of production method is therefore important even given the same type of content.

Comparison of Phytochemical Constituents According to the Cultivation Method (Paddy Field Cultivation, Upland Field Cultivation) of Korean Fresh Ginseng (한국산 수삼의 재배환경(논, 밭)에 따른 식물화학성분 비교)

  • Her, Jae Young;Kim, Do Hyeong;Hwang, Yu Jin;Ko, Sung Kwon;Yang, Byung Wook
    • Journal of Korean Medicine for Obesity Research
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    • v.22 no.1
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    • pp.21-29
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    • 2022
  • Objectives: This study aimed to examine the changes in ingredients according to its cultivation method by examining the content of saponin and non-saponin components of ginseng. Methods: Ginseng saponin component analysis was compared and reviewed using the high-performance liquid chromatography method, and acidic polysaccharide component was measured using the carbazole sulfuric acid method. Results: The comparative analysis of ginseng saponin content of 4 and 6 years old fresh ginseng showed the following results. According to the cultivation method, upland field cultivation fresh ginseng showed higher average content of crude saponin than paddy field cultivation fresh ginseng. Whereas, paddy field cultivation fresh ginseng showed higher average content of total saponin than upland field cultivation fresh ginseng. Ginsenoside Rb1 showed higher content of paddy field cultivation fresh ginseng than upland field cultivation fresh ginseng in 6 years old ginseng. However, it showed higher content of upland field cultivation fresh ginseng than paddy field cultivation fresh ginseng in 4 years old ginseng. Additionally, ginsenoside Rg1 showed higher content of paddy field cultivation fresh ginseng than upland field cultivation fresh ginseng in 6 years old ginseng, whereas upland field cultivation fresh ginseng showed higher content of paddy field cultivation fresh ginseng in 4 years old ginseng. The effect on the content of ginseng saponins and acidic polysaccharides according to paddy field and upland field cultivation methods is considered to be small. Conclusions: The paddy field cultivation method, which is more efficient in production cost due to depletion of farmland and long-distance cultivation, is considered to be an economical cultivation method.

Effect of Cr on Flow Accelerated Corrosion of Carbon Steel (탄소강의 유동가속부식에 미치는 크롬의 영향)

  • Lee, Eun Hee;Kim, Kyung Mo;Kim, Hong Pyo;Kim, Dong Jin
    • Corrosion Science and Technology
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    • v.14 no.1
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    • pp.25-32
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    • 2015
  • The alloy content of structural materials of nuclear power plants has been recognized an important factor in predicting flow accelerated corrosion (FAC). In particular, many literature data reported that chromium content is one of the most important alloying element and even a small amount of chromium is effective to suppress FAC. This report reviewed and compared chromium models of Ducreux, Bouchacourt, and Kastner which were used in predicting FAC rates. The plant data indicate that Ducreux model may be conservative for the specimen containing 0.15 wt% chromium. The related articles were reviewed as follows. Combined effects of chromium content, pH, temperature, dissolved oxygen (DO), flow velocity, test time, and kinds of amine on the FAC rate were described. 0.1 wt% chromium in steel did not affect the FAC rate with changes in pH. The FAC rates pronounced with higher flow rate and increased with increasing test duration(600 d) for 0.013 wt% chromium. The FAC rates in mixed amine chemistry were higher than in ammonia chemistry, which may be lessened by the addition of chromium to the steel.

Post-Infectional Biochemical Changes in Mulberry Due to Xanthomonas campestris pv. mori Induced Bacterial Leaf Spot

  • Maji, M.D.;Sengupta, T.;Das, C.;Urs, S.Raje
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.2
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    • pp.255-259
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    • 2004
  • Post-infectional biochemical changes due to Xanthomonas campestris pv. mori (Xcm) infection in five elite mulberry varieties viz., $S_1$, $S_{1635}$, $V_1$, RF $S_{175}$ and JRH was studied under inoculated condition. It was revealed that total soluble sugar and protein content was significantly declined in all the varieties due to X. campestris infection. Total phenol content was at par prior to inoculation in all varieties, but it was significantly increased in $S_1$, RF $S_{175}$, $S_{1635}$ and JRH 7 days after inoculation. The correlation coefficient (r) between total soluble sugar and total phenol content was found positive (r = 0.825) and statistically significant. Similarly, correlation coefficient (r) between total soluble protein and phenol content was found positive (r = 0.897) and statistically significant. The present study indicates that X. campestris infected leaves are nutritionally inferior in quality and the duration of phenol production in a mulberry variety play decisive role on disease resistance.nce.

Effects of Maturity at Harvest and Wilting Days on Quality of Round Baled Rye Silage

  • Kim, J.G.;Chung, E.S.;Seo, S.;Ham, J.S.;Kang, W.S.;Kim, D.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.9
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    • pp.1233-1237
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    • 2001
  • A study was conducted to determine the effects of maturity at harvest and wilting days on the quality of round baled rye (Secale cereale L.) silage. This study was a $3{\times}3$ factorial arrangement in a split plot design with 3 replicates. The main plot was 3 harvesting dates at the stage of boot (20 Apr.), heading (29 Apr.) and flowering (14 May). The subplot was wilting day : 0 (unwilted), 0.5 and 1 day (0, 1, and 2 days at boot stage). Acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents of rye silage were significantly greater than those of rye before ensiling, but crude protein (CP) content and in vitro dry matter digestibility (IVDMD) were vice versa. Buffering capacity (BC) of rye harvested at flowering stage was decreased from 264 to 202 meq/kg at 1 day wilting, however, it was increased when harvested at boot or heading stage. The pH in wilted silage was the highest while that of flowering stage was the lowest. Water soluble carbohydrate (WSC) content of wilting rye was lower than that of unwilted, and the lowest at late harvesting stage. All plots had minimal WSC content for silage fermentation. Wilting treatment and delayed harvesting date caused an increase in dry matter (DM) content of round bale silage. The content of ammonia-N expressed as a portion of total N showed negative correlation with DM content. High quality silage according to ammonia-N content could be obtained from mid-harvest with wilting. There were highly significant differences in each organic acid between harvesting dates and wilting periods. Acetic and butyric acid contents were increased with delayed harvesting and prolonged wilting period, the lactic acid content, however, was decreased. This study demonstrated that harvest of rye from heading to flowering stage with wilting would be a recommendable method for making high quality rye silage using round bale system.