DOI QR코드

DOI QR Code

Nutritional Components and Physiological Activity of Centella asiatica Cultured in Chungju by Drying Method

건조방법에 따른 충주산 병풀의 영양성분과 생리활성

  • Eom, Hyun-Ju (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Shin, Hyun-Young (Dept. of Integrated Biomedical and Life Science, Korea University) ;
  • Ji, Yeong Mi (Chungju Agricultural Technology and Extension Center) ;
  • Kwon, Nu Ri (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Yoon, Hyang-Sik (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Kim, In Jae (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Song, Yong-sup (Chungcheongbukdo Agricultural Research and Extension Services) ;
  • Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
  • 엄현주 (충청북도농업기술원) ;
  • 신현영 (고려대학교 의생명융합과학과) ;
  • 지영미 (충주시농업기술센터) ;
  • 권누리 (충청북도농업기술원) ;
  • 윤향식 (충청북도농업기술원) ;
  • 김인재 (충청북도농업기술원) ;
  • 송용섭 (충청북도농업기술원) ;
  • 유광원 (한국교통대학교 식품영양학전공)
  • Received : 2021.02.19
  • Accepted : 2021.03.15
  • Published : 2021.04.30

Abstract

Centella asiatica (CA) has been used as a nutritional plant as well as a traditional herbal medicine around the world. This study, quality component (proximate composition, total polyphenol, and triterpenoid compound), and antioxidant and anti-inflammatory activities of CA dried using various methods were investigated. Proximate compositions of CA with different drying methods included a large amount of carbohydrates, crude protein, crude ash, and crude fiber. Among the drying methods, cold drying provided the highest total polyphenol content and antioxidant activity, while hot-air drying at 75℃ provided the lowest total polyphenol content and antioxidant activity. In addition, when the major triterpenoid compounds of CA were analyzed, the highest content of asiaticoside of triterpenoid glycoside was obtained with all drying methods. With respect to the total triterpenoid, the highest content was obtained with cold drying (68.8 mg/g) whereas natural drying (31.4 mg/g) provided the lowest content. In anti-inflammatory activity of LPS-stimulated RAW 264.7 cells, EtOH extract of cold drying showed a significantly higher inhibitory activity in comparison to the other drying methods. In conclusion, it is considered that the cold drying method is suitable for industrial preparation of functional materials with high physiological ingredients, and antioxidant and anti-inflammatory activities from CA.

Keywords

References

  1. Amerine MA, Ough CS. 1980. Methods for Analysis of Musts and Wine, Wiley Sons, pp. 176-180
  2. AOAC. 1990. Official Methods of Analysis. 15th ed. p.342. Association of Official Analytical Chemists
  3. Ariffin F, Chew SH, Bhupinder K, Karim AA Huda N.N2011. Antioxidant capacity and phenolic composition of fermented Centella asiatica herbal teas. J Sci Food Agric 91:2731-2739 https://doi.org/10.1002/jsfa.4454
  4. Bylka W, Znajdek-Awizen P, Studzinska-Sroka E, Brzezinska M. 2013. Centella asiatica in cosmetology. Postepy Dermatol Alergol 1:46-49 https://doi.org/10.5114/pdia.2013.33378
  5. Bylka W, Znajdek-Awizen P, Studzinska-Sroka E, Danczak-Pazdrowsk A, Brzezinska M. 2014. Centella asiatica in dermatology: An overview. Phytother Res 28:1117-1124 https://doi.org/10.1002/ptr.5110
  6. Cha JY, Kim HJ, Chung CH, Cho YS. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J Korean Soc Food Sci Nutr 28:1310-1315
  7. Chandrika UG, Prasad Kumarab PAAS. 2015. Gotu Kola (Centella asiatica): Nutritional properties and plausible health benefits. Adv Food Nutr Res 76:125-157 https://doi.org/10.1016/bs.afnr.2015.08.001
  8. Choi Y, Kim MH, Shin JJ, Park JM, Lee J. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32:723-727 https://doi.org/10.3746/JKFN.2003.32.5.723
  9. Goo YM, Kil YS, Sin SM, Lee DY, Jeong WM, Ko K, Yang K, Kim YH, Lee SW. 2018. Analysis of anti-bacterial, antiinflammatory, and skin-whitening effect of Centella asiatica (L.) Urban. J Plant Biotechnol 45: 117-124 https://doi.org/10.5010/JPB.2018.45.2.117
  10. Ha JH, Kwon MC, Kim Y, Jeong SS, Jeong MH, Hwang B, Lee HY. 2009. Enhancement of immuno-modulatory of Centella asiatica L. Urban with edible polymer through nano-encapsulation process. Korean J Med Crop Sci 17:257-265
  11. Jeon HJ, Kang HJ, Jung HJ, Kang YS, Lim CJ, Kim YM, Park EH. 2008. Anti-inflammatory activity of Taraxacum officinale. J Ethnopharmacol 115:82-88 https://doi.org/10.1016/j.jep.2007.09.006
  12. Jeon YJ, Han SH, Lee YW, Lee M, Yang KH, Kim HM. 2000. Dexamethasone inhibits IL-1β gene expression in LPS stimulated RAW 264.7 cells by blocking NF-κB/Rel and AP-1 activation. Immunopharmacology 48: 173-183 https://doi.org/10.1016/S0162-3109(00)00199-5
  13. Kil YS, Sin SM, Lee DY, Min JW, Yang KJ, Lee SW, Kim YH, Goo YM. 2018. Analysis triterpene glycoside levels and antioxidant activity in the different shoot tissues of Centella asiatica (L.) Urban. J Life Sci 28:917-922 https://doi.org/10.5352/JLS.2018.28.8.917
  14. Kim AR, Lee HJ, Jung HO, Lee JJ. 2014. Physicochemical composition of ramie leaf according to drying methods. J Korean Soc Food Sci Nutr 43:118-127 https://doi.org/10.3746/JKFN.2014.43.1.118
  15. Kim OT, Kim MY, Park YJ, Hong MH, Ahn JC, Oh MH, Hwang B. 2002. Production of triterpene glycosides form whole plant cultures of Centella asiatica (L.) Urban. Korean J Plant Biotechnol 29:281-285 https://doi.org/10.5010/JPB.2002.29.4.281
  16. Kwon MC, Han JG, Ha JH, Oh SH, Jin L, Jeong HS, Choi GP, Hwang B, Lee HY. 2008. Immuno-regulatory effect on Centella asiatica L. Urban extraction solvent associated with ultrasonification process. Korean J Med Crop Sci 16:294-300
  17. Lee JM. 2016. Physicochemical and antioxidant properties in Allium hookeri by hot airand freeze-drying methods. Korean J Food Preserv 23:57-62 https://doi.org/10.11002/KJFP.2016.23.1.57
  18. Lee YH, Choi HK, N'deh KPU, Choi YJ, Fan M, Kim EK, Chumg KH, An JH. 2020. Inhibitory effect of Centella asiatica extract on DNCB-induced atopic dermatitis in HaCaT cells and BALB/c mice. Nurtrients 12:411 https://doi.org/10.3390/nu12020411
  19. Lee YY, Kang SA. 2020. Antioxidative and anti-inflammatory activities of Salvia plebeia R. Br extracts. Korean J Food Nutr 33:483-492 https://doi.org/10.9799/KSFAN.2020.33.5.483
  20. Ministry of Food and Drug Safety. 1998. List of food ingredients. Available from http://www.foodsafetykorea.go.kr/portal/safefoodlife/foodMeterial/foodMeterialDB.do [cited 8 February 2021]
  21. Mohd Razali NN, Ng CT, Fong LY. 2019. Cardiovascular protective effects of Centella asiatica and its triterpenes: A review. Planta Med 85:1203-1215 https://doi.org/10.1055/a-1008-6138
  22. Ogunka-Nnoka CU, Igwe FU, Agwu J, Peter OJ, Wolugbom PH. 2020. Nutrient and phytochemical composition of Centella asiatica leaves. Med Aromat Plants 9:1-7
  23. Pittella F, Dutra RC, Junior DD, Lopes MTP, Barbosa NR. 2009. Antioxidant and cytotoxic activities of Centella asiatica (L) Urb. Int J Mol Sci 10:3713-3721 https://doi.org/10.3390/ijms10093713
  24. Rafamantanana MH, Rozet E, Raoelison GE, Cheuk K, Ratsimammanga SU, Hubert P, Quetin-Leclercq J. 2009. An improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycone in leaves of Centella asiataca (L.) Urb (Apiaceae). J Chromatogr B 877:2396-2402 https://doi.org/10.1016/j.jchromb.2009.03.018
  25. Roberty R, Anna P, Anna P, Catherine RE, Min P, Icoletta P. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  26. Shan J, Fu J, Zhao Z, Kong X, Huang H, Luo L, Yin Z. 2009. Chlorogenic acid inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in RAW264.7 cells through suppressing NF-kappaB and JNK/AP-1 activation. Int Immunopharmacol 9:1042-1048 https://doi.org/10.1016/j.intimp.2009.04.011
  27. Shin HY, Kim H, Jeong EJ, Kim JE, Lee KH, Bae YJ, Yu KW. 2020. Bioactive compounds, anti-oxidant activities and anti-inflammatory activities of solvent extracts from Centella asiatica cultured in Chungju. Korean J Food Nutr 33:692-701 https://doi.org/10.9799/KSFAN.2020.33.6.692
  28. Zainol MK, Abd-Hamid A, Yusof S, Muse R. 2003. Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.) Urban. Food Chem 81:575-581 https://doi.org/10.1016/S0308-8146(02)00498-3