• Title/Summary/Keyword: content analyses

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Estimation of Pork Quality Traits Using Exsanguination Blood and Postmortem Muscle Metabolites

  • Choe, J.H.;Choi, M.H.;Ryu, Y.C.;Go, G.W.;Choi, Y.M.;Lee, S.H.;Lim, K.S.;Lee, E.A.;Kang, J.H.;Hong, K.C.;Kim, B.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.6
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    • pp.862-869
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    • 2015
  • The current study was designed to estimate the pork quality traits using metabolites from exsanguination blood and postmortem muscle simultaneously under the Korean standard pre- and post-slaughter conditions. A total of 111 Yorkshire (pure breed and castrated male) pigs were evaluated under the Korean standard conditions. Measurements were taken of the levels of blood glucose and lactate at exsanguination, and muscle glycogen and lactate content at 45 min and 24 h postmortem. Certain pork quality traits were also evaluated. Correlation analysis and multiple regression analysis including stepwise regression were performed. Exsanguination blood glucose and lactate levels were positively correlated with each other, negatively related to postmortem muscle glycogen content and positively associated with postmortem muscle lactate content. A rapid and extended postmortem glycolysis was associated with high levels of blood glucose and lactate, with high muscle lactate content, and with low muscle glycogen content during postmortem. In addition, these were also correlated with paler meat color and reduced water holding capacity. The results of multiple regression analyses also showed that metabolites in exsanguination blood and postmortem muscle explained variations in pork quality traits. Especially, levels of blood glucose and lactate and content of muscle glycogen at early postmortem were significantly associated with an elevated early glycolytic rate. Furthermore, muscle lactate content at 24 h postmortem alone accounted for a considerable portion of the variation in pork quality traits. Based on these results, the current study confirmed that the main factor influencing pork quality traits is the ultimate lactate content in muscle via postmortem glycolysis, and that levels of blood glucose and lactate at exsanguination and contents of muscle glycogen and lactate at postmortem can explain a large portion of the variation in pork quality even under the standard slaughter conditions.

Proximate Analysis and Antioxidant Activity of Cultivated Wild Panax ginseng (연근별 산양삼의 일반성분 및 항산화 효과)

  • Lee, Geun;Choi, Gwang-Su;Lee, Ju-Yeol;Yun, So-Jung;Kim, Wooki;Lee, Hyungjae;Baik, Moo-Yeol;Hwang, Jae-Kwan
    • Food Engineering Progress
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    • v.21 no.3
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    • pp.208-214
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    • 2017
  • Proximate analysis and antioxidant activity of cultivated wild Panax ginseng (CWPG) were investigated to provide fundamental information of CWPG with different ages and to increase its industrial application. Proximate analyses of CWPG with different ages were performed. Extraction of CWPG with different ages was carried out using heat-reflux extraction, and their extraction yield, crude saponin content, ginsenoside content, and antioxidant activity were analyzed. Moisture content decreased, but crude fat and crude protein were increased with aging. Extraction yield and crude saponin contents did not show a specific pattern while 5-year-old CWPG revealed the highest extraction yield and crude saponin content. All CWPGs showed typical ginsenoside profiles containing C-K and Rh2 ginsenosides, which are not found in ginseng. The 3-year-old CWPG showed the highest antioxidant activity including total phenolic content, total flavonoid content, and DPPH and ABTS radical scavenging activities. Moreover, 3-year-old CWPG also revealed the highest acidic polysaccharide content. Therefore, these results suggested that 3-year-old CWPG, which is the cheapest, can be used in industrial application due to its high antioxidant activity and acidic polysaccharide content with similar ginsenoside profile compared to 5- and 7-year-old CWPGs.

Studies on the Prediction of the Shelf-life of Kochujang through the Physicochemical and Sensory Analyses during Storage (고추장 저장 중 이화학 및 관능적 특성에 의한 유통기간 예측에 대한 연구)

  • Lee, Ki-Young;Kim, Hyung-Suk;Lee, Hyeon-Gyu;Han, Ouk;Chang, Un-Jae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.4
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    • pp.588-594
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    • 1997
  • In this study, the shelf-life of Kochujang during storage was predicted through physicochemical and sensory analyses. Amino nitrogen, lightness, characteristics of surface color, pH and number of viable cell counts in Kochujang decreased during storage, while ammonia nitrogen, titratable acidity and viscosity increased. Among the physicochemical analyses, amino nitrogen content exhibited the highest correlation with sensory score. The marginal amounts of amino nitrogen was 170.6mg%. Degradation rate of amino nitrogen was a first order reaction. Q$_{10}$-value and the activation energy of Kochujang during storage were 1.80 and 8.6kca1/mol, respectively. The shelf-life Predicted of Kochujang at each storage temperature was calculated. The shelf-life predicted was 467 days at 1$0^{\circ}C$, 261 days at 2$0^{\circ}C$ and 133 days at 35$^{\circ}C$.

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Chracteristics of Primary Health Practice and Diagnosis-Cluster Pattern in Health Insurance (의원의 특성에 따른 상병진단군의 분포에 대한 연구)

  • Yoon, Jong-Ryool;Moon, Ok-Ryun;Huh, Jung;Kim, Chang-Yup
    • Health Policy and Management
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    • v.3 no.2
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    • pp.100-129
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    • 1993
  • This study is designed to find out some intra-clinic factors affecting the content of practice provided by primary care physicians in Korea, and proposed factors in this study are characteristcs of each private clinc --- physician-related variables(age, sex, specialty), bfed-related variables for inpatient care, laboratory-related variables for precise diagnosis. We have tried to estimate the difference of disease entities cared by each primary care physician according to above factors by analyzin gdisease data claimed during one month(April, 1992) to National Federation of Medical Insurance. The diagnosis codes by ICD-9 in the research disease data were reclassified to 'diagnosis clusters' by virtue of clinical similarities for effective analyses. We have converted frequent-tsing ICD-9 codes to 86 diagnosis clusters, which incorporated 97.4 percents of all ambulatory visits to private clinics. This result means proposed diagnosis-cluster method is effective tool for analysis of the content of ambulatory medical care carried out by primary care physicians. Comparisons and analyses of multiple diagnosis-clusters made on the basis of presented factors were done and the results were as follows; - Major factors affecting the difference between diagnosis-cluster pattern by each variables were phyusician's age, sex, specialty and bed counts of each private clinic for inpatient care and the size of laboratories of each clinic. - Middle aged(30th to 40th) group physicians are providing more comprehensive care than 20th or above 50th aged groups. Male physicians are more adequate for comprehensive care than female physicians, because woman-doctors are providing narrow-spectrum care. The content of practice of obstetricians and gynecologists shows much difference from primary medical practice, and they cannot be included in primary care physician, this study suggested. Pediatricians are also providing short-spectum acre, and nearly all visits to pediatricians were incorporated only 2-3 diagnosis-clusters. General surgeons' practices are very similar to general practioners' or family physicians' practices, the means they are providing primary care rather than special surgical care. And small number of beds(under 5 beds) and only basic(2-3 sorts of)diagnostic apparatuses are sufficient for primary physicians' clinic to carry out primary care. In conclusion, to reinforce primary care department in Korea, there must be support with health policy to expand office-based primary care practice-- with small number of beds for inpatient care and only basic laboratories-- provided by general practitioner of family physician.

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Genetic Stock Identification of Spotted Flounder, Verasper variegatus from Yeocheun, Korea (범가자미에 대한 유전학적 동정)

  • KIM Kyung Kil;KIM Yoon;NAM Yoon Kwan;KIM Dong Soo
    • Journal of Aquaculture
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    • v.6 no.3
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    • pp.221-233
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    • 1993
  • Cell size, DNA content, chromosome and PCR-based mitochondrial 12S rRNA gene analyses were conducted to obtain basic informations for genetic stock identification of spotted flounder (Verasper variegatus) from Yeocheun, Korea. The mean erythrocytic and nuclear volumes of spotted flounder were $211.10{\mu}m^3$ and $23.03{\mu}m^3$, respectively. The haploid DNA content of this species was 0.79 pg/cell which correspond to $46.5\%$ of carp and to $22.6\%$ of mammals. Spotted flounder had the 2n = 46 acrocentric chromosomes but no heteromorphic sex chromosomes was found. Mitochondrial DNA gene for 12S ribosomal RNA was amplified by polymerase chain reaction (PCR) and the PCR products were subjected to digestion with 15 restriction endonucleases. Restriction enzyme analyses revealed that Ava I, Mae II, Sma I and Xba I had one restriction site in the mitochondrial 12S rRNA gene segment of spotted flounder, while Mae I had two. Segments of 12S rRNA gene from mitochondria in spotted flounder were sequenced and compared with channel catfish and human as controls. The 12S rRNA gene of this species was more similar to that of channel catfish than to human's.

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UV-Vis and ED-XRF Analyses of Natural Black Colored Pearls from Freshwater Cultured Shells (UV-Vis와 ED-XRF를 이용한 자연 색상의 담수 흑 양식진주 분석)

  • Kim, Hea-Yeon;Park, Jong-Wan
    • The Korean Journal of Malacology
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    • v.24 no.3
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    • pp.243-251
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    • 2008
  • Analyses of UV-Vis spectrum and reflectance are useful tools to identify pearls of which color was naturally made or chemically changed. Contents of some trace elements of pearls by using ED-XRF may give us the information that the pearls were originated from marine shells or freshwater shells, and may give us whether the pearls were chemically treated or not. Three types of chemically untreated pearls, freshwater cultured, Akoya cultured and Tahitian cultured, were treated with silver nitrate. UV-Vis absorbance spectrum of Ag was changed after treatment, and reflectance of pearls was decreased. Absorbance of natural black colored Tahitian cultured pearls at 400, 500 and 700 nm was observed. Natural black colored freshwater cultured pearls showed two characteristic absorption patterns in UV-Vis spectra at 380-400, 480-500 nm. Unlike naturally black colored Tahitian cultured pearls, the absorption spectrum at 700 nm could not be found from black freshwater cultured pearls. Manganese was not detected in the pearls from marine shells, and strontium content of the pearls from marine cultured shells was higher than that from freshwater cultured shells. According to ED-XRF analysis, Ag contents of silver nitrate treated pearls increased as more time passed, because silver ion invaded to nacre layer of the pearls. Since Ag content of the black pearls known as untreated ranged under 0.1%, analysis of Ag content may used to distinguish the black pearls are treated or not in marketplace.

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Content Analyses of Green Life-Teaching Materials in Korean and Japanese High School Home Economics Textbooks (한.일 고등학교 가정교과서 녹색생활 학습자료 분석)

  • Cho, Jae-Soon;Park, Mi-Jeong;Jang, Sang-Ock;Lee, Yon-Suk
    • Journal of Korean Home Economics Education Association
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    • v.23 no.1
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    • pp.19-36
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    • 2011
  • The purpose of this paper was to explore the teaching materials in the Korean and Japanese high school Home Economics textbooks from the perspective of green life education. Although sustainable living at home has been emphasized as one of the main issues of Home Economics since the first curriculum period, green growth education has reinforced in the 2009 revised curriculum. The content analyses had been done to explore the teaching materials of five areas in the 6 Korean and 4 Japanese high school Home Economics textbooks through two checking steps. The material form of table-figure-pictures was the most frequently shown in both Korean and Japanese textbooks. Green life-teaching materials were more likely in the area of household management/consumer activity than in any other ones. All textbooks of both countries have included the green life-teaching materials according to the elements of contents mentioned in the curricula. The quantity and contents of the materials were some what differ by textbooks, so home economics teacher need to cross check the all textbooks to select useful information although the textbooks have included various types of green materials over the 5 areas. Home Economics curriculum need to actively and concretely include the green growth educational elements suggested by the previous study.

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Content Analyses of Green Life-Educational Materials in Korean and Japanese Middle School Horne Economics Textbooks (한.일 중학교 가정교과서의 녹색생활교육 내용분석)

  • Park, Mi-Jeong;Cho, Jae-Soon;Jang, Sang-Ock;Lee, Yon-Suk
    • Journal of Korean Home Economics Education Association
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    • v.23 no.1
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    • pp.1-17
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    • 2011
  • The purpose of this paper was to explore the materials in the Korean and Japanese Home Economics textbooks in middle school with the perspective of green life education. Home Economics is one of the relevant subjects to green growth education. Therefore, home economics teachers need to teach green life based on the low $CO_2$-green growth in the class. Sustainable living at home has been emphasized as one of the main importance of Home Economics since the first curriculum period. However, it is required to reinforce in the 2009 revised curriculum. The content analyses had been done to explore the materials of five areas in the 8 Korean and 2 Japanese junior highschool Home Economics textbooks through two checking steps. Each subject area of Home Economics had various form of materials such as terminology, table figure pictures, reading, and activity, related to green life related to environmental, economic, and social sustenance. The forms and the quantity of materials were some what differ by subject areas as well as textbooks. The levels of material contents were ranged from the knowledge, recognition, to intention to practicum of green life. The green life materials were more likely practical in Japanese textbooks rather than Korean ones. It is recommended teachers to choose the valuable materials over the textbooks and practical green life should clearly mentioned in home economics curriculum to be showed in textbooks.

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Physiological and Biochemical Analyses of Rice Sensitivities to UVB Radiation

  • Hidema, Jun;Kumagai, Tadashi
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.162-165
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    • 2002
  • Rice is widely cultivated in various regions throughout Asia. Over a five-year period, we investigated the effects of supplemental UVB radiation on the growth and yield of Japanese rice cultivars in the field. The findings of that study indicated that supplemental UVB radiation has inhibitory effects on the growth and grain development. Furthermore, we investigated the sensitivity to UVB radiation of rice cultivars of 5 Asian rice ecotypes, and found that rice cultivars vary widely in UVB sensitivity. The aim of our study is improving UVB resistance in plants by bioengineering or breeding programs. In order to make it, there is need to find the molecular origin of the sensitivity to UVB. Cyclobutane pyrimidine dimer (CPD) is major UV-induced DNA lesions. Plants possess two mechanisms to cope with such DNA damage. The first is the accumulation of UV-absorbing compounds. Our previous data showed that the steady-state CPD levels in leaves of rice grown under chronic radiation in any culture were not so greatly influenced by the increased UV-absorbing compounds content, although there was a significant positive correlation between the CPD levels induced by challenge UVB exposure and the UV-absorbing compounds content. The other is the repair of DNA damage. Photorepair is the major pathway in plants for repairing CPD. We found that the sensitivity to UVB could seriously correlate with the low ability in CPD photorepair in rice plants. These results suggest that photo lyase might be an excellent candidate for restoration by way of selective breeding or engineering in rice.

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The Effects of Blend Composition and Blending Time on the Ester Interchange Reaction and Tensile Properties of PLA/LPCL/HPCL Blends

  • Yoon, Cheol-Soo;Ji, Dong-Sun
    • Fibers and Polymers
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    • v.4 no.2
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    • pp.59-65
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    • 2003
  • PLA/LPCL/HPCL blends composed of poly(lactic acid) (PLA), low molecular weight poly($\varepsilon$-caprolactone) (LPCL), and high molecular weight poly($\varepsilon$-caprolactone) (HPCL) were prepared by melt blending for bioabsorbable fila-ment sutures. The effects of blend composition and blending time on the ester interchange reaction by alcoholysis in the PLA/LPCL/HPCL blends were studied. Their thermal properties and the miscibility due to the ester interchange reaction were investigated by $^1{H-NMR}$, DSC, X-ray, and UTM analyses. The hydroxyl group contents of LPCL in the blends decreafed by the ester interchange reaction due to alcoholysis. Thus, the copolymer was formed by the ester interchange reaction at $200^{\circ}C$ for 30-60 minutes. The thermal properties of PLA/LPCL/HPCL blends such as melting temperature and heat of fusion decreased with increasing ester interchange reaction levels. However, the miscibility among the three poly-mers was improved greatly by ester interchange reaction. Tensile strength and modulus of PLA/LPCL/HPCL blend fibers increased with increasing HPCL content, while the elongation at break of the blend fibers increased with increasing LPCL content.