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연근별 산양삼의 일반성분 및 항산화 효과

Proximate Analysis and Antioxidant Activity of Cultivated Wild Panax ginseng

  • 이근 (연세대학교 생물소재공학협동과정) ;
  • 최광수 (경희대학교 식품생명공학과) ;
  • 이주열 (경희대학교 식품생명공학과) ;
  • 윤소정 (단국대학교 식품공학과) ;
  • 김우기 (경희대학교 식품생명공학과) ;
  • 이형재 (단국대학교 식품공학과) ;
  • 백무열 (경희대학교 식품생명공학과) ;
  • 황재관 (연세대학교 생물소재공학협동과정)
  • Lee, Geun (Graduate Program in Biomaterials Science and Engineering, Yonsei University) ;
  • Choi, Gwang-Su (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lee, Ju-Yeol (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Yun, So-Jung (Department of Food Engineering, Dankook University) ;
  • Kim, Wooki (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Lee, Hyungjae (Department of Food Engineering, Dankook University) ;
  • Baik, Moo-Yeol (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hwang, Jae-Kwan (Graduate Program in Biomaterials Science and Engineering, Yonsei University)
  • 투고 : 2017.05.30
  • 심사 : 2017.06.13
  • 발행 : 2017.08.31

초록

산양삼의 연근별 특징을 분석한 결과 3년근에서는 ginsenoside 등의 성분이 활발하게 생성되고, 연령이 늘어갈 수록 부피 성장이 중점적으로 일어나는 것으로 나타났다. 또한 산성다당체 함량, 총 페놀 함량, 총 플라보노이드 함량 및 항산화능 모두 3년근 산양삼이 다른 연근보다 유의적(p<0.05)으로 높은 결과 값을 나타내었다. 결과적으로 3년근의 경우 단위 중량당 더 많은 양의 성분들을 가지고 있으므로 파종 후 장기간 재배보다는 단시간에 수확하는 것이 재배를 하는 임업인이나 제품을 개발하는 기업인 모두에게 유리 할 것으로 판단된다. 따라서 ginsenoside의 함량 및 다른 생리활성 성분들의 양으로 연근별 산양삼의 특징을 비교 할 경우 비록 부피는 작지만 가격이 싸고 개체수가 많은 3년근을 이용하여 제품을 개발하는 것이 산업적으로 이득이 될 수 있을 것으로 판단된다. 본 연구의 결과는 평창산 산양삼에 한정된 항산화 결과로서 국내 다른 지역에서 자생하는 연근별 산양삼에 대한 세부적인 기능성 연구가 추가적으로 필요한다.

Proximate analysis and antioxidant activity of cultivated wild Panax ginseng (CWPG) were investigated to provide fundamental information of CWPG with different ages and to increase its industrial application. Proximate analyses of CWPG with different ages were performed. Extraction of CWPG with different ages was carried out using heat-reflux extraction, and their extraction yield, crude saponin content, ginsenoside content, and antioxidant activity were analyzed. Moisture content decreased, but crude fat and crude protein were increased with aging. Extraction yield and crude saponin contents did not show a specific pattern while 5-year-old CWPG revealed the highest extraction yield and crude saponin content. All CWPGs showed typical ginsenoside profiles containing C-K and Rh2 ginsenosides, which are not found in ginseng. The 3-year-old CWPG showed the highest antioxidant activity including total phenolic content, total flavonoid content, and DPPH and ABTS radical scavenging activities. Moreover, 3-year-old CWPG also revealed the highest acidic polysaccharide content. Therefore, these results suggested that 3-year-old CWPG, which is the cheapest, can be used in industrial application due to its high antioxidant activity and acidic polysaccharide content with similar ginsenoside profile compared to 5- and 7-year-old CWPGs.

키워드

과제정보

연구 과제 주관 기관 : 농림축산식품부

참고문헌

  1. Ando T, Tanaka O, Shibata S. 1971. Chemical studies on the oriental plant drugs. (XXY) Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Yakugaku Zasshi 25: 28-32.
  2. AOAC, 2002. Official methods of analysis of AOAC International 17th edition, Arlington, VA, USA.
  3. Bae MJ, Kim SJ, Ye EJ, Nam HS, Park EM. 2009. Antioxidant activity of tea made from Korean mountain-cultivated leaves and its influence on lipid metabolism. Korean J. Food Culture 24: 77-83.
  4. Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28: 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
  5. Byeon SE, Yi YS, Oh J, Yoo BC, Hong S, Cho JY. 2012. The role of Src kinase in macrophage-mediated inflammatory responses. Mediaors Inflamm. 2012.
  6. Do JH, Lee HO, Lee SK, Jang JK, Lee SD, Sung HS. 1993. Colorimetric determination of acidic polysaccharide from Panax ginseng, its extraction condition and stability. Korean J. Ginseng Sci. 17: 139-144.
  7. Gerschman R, Gilbert DL, Nye SW, Dwyer P, Fenn WO. 1954. Oxygen poisoning and x-irradiation: a mechanism in common. Science 119: 623-626. https://doi.org/10.1126/science.119.3097.623
  8. Han JS, Tak HS, Lee GS, Kim JS, Woo RJ, Choi JE. 2013a. Comparison of ginsenoside content and ratio of root tissue according to root age and diameter in Panax ginseng C. A. Meyer. Korean J. Med. Crop Sci. 21: 342-347. https://doi.org/10.7783/KJMCS.2013.21.5.342
  9. Han JS, Tak HS, Lee KS, Kim JS, Choi JE. 2013b. Comparison of ginsenoside content according to age and diameter in Panax ginseng C. A. Meyer cultivated by direct seeding. Korean J. Med. Crop Sci. 21: 184-190. https://doi.org/10.7783/KJMCS.2013.21.3.184
  10. Jang HY, Park HS, Kwon KR, Rhim TJ. 2008. A study on the comparison of antioxidant effects among wild ginseng, cultivated wild ginseng, and cultivated ginseng extracts. J. Pharmacopuncture 11: 67-78. https://doi.org/10.3831/KPI.2008.11.3.067
  11. Kang KS, Yamabe N, Kim HY, Park JH, Yokozawa T. 2008. Therapeutic potential of 20(S)-ginsenoside Rg(3) against streptozotocin-induced diabetic renal damage in rats. Eur. J. Pharmacol. 591: 266-272 https://doi.org/10.1016/j.ejphar.2008.06.077
  12. Kim DO, Jeong SW, Lee CY. 2003. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 81: 321-326. https://doi.org/10.1016/S0308-8146(02)00423-5
  13. Kim JH, Kim JK. 2005. Effect of extracting conditions on chemical compositions of Korean mountain ginseng extract. J. Korean Soc. Food Sci. Nutr. 34: 862-868. https://doi.org/10.3746/jkfn.2005.34.6.862
  14. Kim KT, Yoo KM, Lee JW, Eom SH, Hwang IK, Lee CY. 2007. Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J. Ethnopharmacol. 111: 443-450. https://doi.org/10.1016/j.jep.2007.01.004
  15. Lee CR, Whang WK, Shin CG, Lee HS, Han ST, Im BO, Ko SK. 2004. Comparison of ginsenoside composition and contents in fresh ginseng roots cultivated in Korea, Japan, and China at various ages. Korean J. Food Sci. Technol. 36: 847-850.
  16. Lee HJ, Yoo BS, Byun SY. 2000. Differences in free amino acids between Korean ginsengs and mountain ginsengs. Korean J. Biotechnol. Bioeng. 15: 323-328.
  17. Li XG, Kang SJ, Han JS, Kim JS, Choi JE. 2009. Effects of root diameter within different root parts on ginsenoside composition of Yunpoong cultivar in Panax ginseng C. A. Meyer. Korean J. Med. Crop Sci. 17: 452-457.
  18. McCord JM, Fridovich I. 1969. Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049-6055.
  19. Namba T, Yoshizaki M, Tomimori T, Kobashi K, Mitsui K. 1974. Fundamental studies on the evaluation of the crude drugs. 3. Chemical and biochemical evaluation of ginseng and related crude drugs (author's transl). Yakugaku Zasshi 94: 252-260. https://doi.org/10.1248/yakushi1947.94.2_252
  20. Pan HY, Qu Y, Zhang JK, Kang TG, Dou DQ. 2013. Antioxidant activity of ginseng cultivated under mountainous forest with different growing years. J. Ginseng Res. 37: 355-360. https://doi.org/10.5142/jgr.2013.37.355
  21. Shin JY, Song JY, Yun YS, Yang HO, Rhee DK, Pyo S. 2002. Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function. Immunopharmacol. Immunotoxicol. 24: 469-482. https://doi.org/10.1081/IPH-120014730
  22. Singleton VL, Orthofer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Method. Enzymol. 299: 152-178.
  23. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39: 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  24. Yang BW, Im BO, Ko SK. 2006. Comparison of non-saponin composition and contents in fresh ginseng roots cultivated in different areas and at various ages. Yakhakhoe Chi. 50: 215-219.
  25. Yoo BS, Lee HJ, Byun SY. 2000. Differences in phenolic compounds between Korean ginseng and mountain ginseng. Korean J. Biotechnol. Bioeng. 15: 120-124.
  26. Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2

피인용 문헌

  1. Ginsenoside contents and antioxidant activities of cultivated mountain ginseng (Panax ginseng C.A. Meyer) with different ages vol.26, pp.1, 2019, https://doi.org/10.11002/kjfp.2019.26.1.90
  2. Global Trends in Research on Wild-Simulated Ginseng: Quo Vadis? vol.12, pp.6, 2021, https://doi.org/10.3390/f12060664
  3. Retraction to: Inhibitory activity against biological enzyme and anti-microbial activity of phenolics from Sambucus sieboldiana var. pendula Leaves vol.64, pp.2, 2017, https://doi.org/10.3839/jabc.2021.063
  4. Changes in the Growth Characteristics and Compound Contents of 2-Year Old Ginseng according to Chitosan and Ultraviolet Light Treatment vol.29, pp.4, 2021, https://doi.org/10.7783/kjmcs.2021.29.4.253
  5. Comprehensive comparison of nutritional constituents and antioxidant activity of cultivated ginseng, mountain-cultivated ginseng, and whole plant parts of mountain-cultivated ginseng vol.64, pp.4, 2017, https://doi.org/10.3839/jabc.2021.064