• 한국마이크로전자및패키징학회:학술대회논문집
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• 한국마이크로전자및패키징학회 2002년도 춘계 기술심포지움 논문집
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• pp.79-84
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• 2002

The bottom-leaded plastic(BLP) packages have attracted substantial attention since its appearance in the electronic industry. Since the solder materials have relatively low creep resistance and are susceptible to low cycle fatigue, the life of the solder joints under the thermal loading is a critical issue for the reliability The represent study established a finite element model for the analysis of the solder joint reliability under thermal cyclic loading. An elasto-plastic constitutive relation was adopted for solder materials in the modeling and analysis. A 28-pin BLP assembly is modeled to investigate the effects of various epoxy molding compound, leadframe materials on solder joint reliability. The fatigue life of solder joint is estimated by the modified Coffin-Hanson equation. The two coefficients in the equation are also determined. A new design for lead is also evaluated by using finite element analysis. Parametric studies have been conducted to investigate the dependence of solder joint fatigue life on various package materials.

• Steel and Composite Structures
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• 제42권4호
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• pp.569-590
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• 2022

This paper presents a combined experimental and numerical study on stainless steel end-plate connections, with an emphasis placed on their ultimate behaviour and rotation capacity. In the experimental phase, six connection specimens made of austenitic and lean duplex stainless steels are tested under monotonic loads. The tests are specifically designed to examine the close-to-failure behaviour of the connections at large deformations. It is observed that the rotation capacity is closely related to fractures of the stainless steel bolts and end-plates. In the numerical phase, an advanced finite element model suitable for fracture simulation is developed. The incorporated constitutive and fracture models are calibrated based on the material tests of stainless steel bolts and plates. The developed finite element model exhibits a satisfactory accuracy in predicting the close-to-failure behaviour of the tested connections. Finally, the moment resistance and rotation capacity of stainless steel end-plate connections are assessed based on the experimental tests and numerical analyses.

• 소성∙가공
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• 제14권8호통권80호
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• pp.681-688
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• 2005

The high-temperature deformation mechanisms of a ${\alpha}+{\beta}$ titanium alloy (Ti-6Al-4V), near-a titanium alloy (Ti-6.85Al-1.6V) and a single-phase a titanium alloy (Ti-7.0Al-1.5V) were deduced within the framework of inelastic-deformation theory. For this purpose, load relaxation tests were conducted on three alloys at temperatures ranging from 750 to $950^{\circ}C$. The stress-versus-strain rate curves of both alloys were well fitted with inelastic-deformation equations based on grain matrix deformation and grain-boundary sliding. The constitutive analysis revealed that the grain-boundary sliding resistance is higher in the near-${\alpha}$ alloy than in the two-phase ${\alpha}+{\beta}$ alloy due to the difficulties in relaxing stress concentrations at the triple-junction region in the near-${\alpha}$ alloy. In addition, the internal-strength parameter (${\sigma}^*$) of the near-${\alpha}$ alloy was much higher than that of the ${\alpha}+{\beta}$ alloy, thus implying that dislocation emission/ slip transfer at ${\alpha}/{\alpha}$ boundaries is more difficult than at ${\alpha}/{\beta}$ boundaries.

• Computers and Concrete
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• 제25권5호
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• pp.383-400
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• 2020

Due to higher stiffness to weight, higher corrosion resistance, higher strength to weight ratios and good durability, concrete composite structures provide many advantages as compared with conventional materials. Thus, they have wide applications in the field of concrete construction. This research focuses on the structural behavior of steel-tube CFRP confined concrete (STCCC) columns under axial concentric loading. A nonlinear finite element analysis (NLFEA) model of STCCC columns was simulated using ABAQUS which was then, calibrated for different material and geometric models of concrete, steel tube and CFRP material using the experimental results from the literature. The comparative study of the NLFEA predictions and the experimental results indicated that the proposed constitutive NLFEA model can accurately predict the structural performance of STCCC columns. After the calibration of NLFEA model, an extensive parametric study was performed to examine the effects of different critical parameters of composite columns such as; (i) unconfined concrete strength, (ii) number of CFRP layers, (iii) thickness of steel tube and (iv) concrete core diameter, on the axial load capacity. Furthermore, a large database of axial strength of 700 confined concrete compression members was developed from the previous researches to give an analytical model that predicts the ultimate axial strength of composite columns accurately. The comparison of the predictions of the proposed analytical model was done with the predictions of 216 NLFEA models from the parametric study. A close agreement was represented by the predictions of the proposed constitutive NLFEA model and the analytical model.

• BMB Reports
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• 제47권5호
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• pp.274-279
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• 2014

Bortezomib has been known as the most promising anti-cancer drug for multiple myeloma (MM). However, recent studies reported that not all MM patients respond to bortezomib. To overcome such a stumbling-block, studies are needed to clarify the mechanisms of bortezomib resistance. In this study, we established a bortezomib-resistant cell line (U266/velR), and explored its biological characteristics. The U266/velR showed reduced sensitivity to bortezomib, and also showed cross-resistance to the chemically unrelated drug thalidomide. U266/velR cells had a higher proportion of CD138 negative subpopulation, known as stem-like feature, compared to parental U266 cells. U266/velR showed relatively less inhibitory effect of prosurvival NF-${\kappa}B$ signaling by bortezomib. Further analysis of RNA microarray identified genes related to ubiquitination that were differentially regulated in U266/velR. Moreover, the expression level of CD52 in U266 cells was associated with bortezomib response. Our findings provide the basis for developing therapeutic strategies in bortezomib-resistant relapsed and refractory MM patients.

• 한국지반환경공학회 논문집
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• 제13권3호
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• pp.85-90
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• 2012

본 논문은 ABAQUS 유한 요소 프로그램을 사용하여 다층지반(화강풍화토-점토-화강풍화토)에서의 외말뚝과 군말뚝의 횡하중에 대한 영향을 설명한다. 본 연구에서의 변수는 캡이 없는 외말뚝과 캡이 있는 군말뚝으로, 파일의 직경은 0.5m, 길이는 10m인 말뚝을 사용하였다. 수치 해석은 말뚝의 간격을 (s=3D, 4D, 5D) 변화시켜 외말뚝과 군말뚝의 거동을 비교하기 위하여 수행되었다. $1{\times}3$군말뚝(leading pile, middle pile, trail pile)은 각각의 말뚝의 저항분포와 수평저항력을 조사하기 위하여 선정되었다. 점토의 해석모델은 Druker-Prager 구성관계를 이용하였고, 화강풍화토의 물성치는 기존의 논문을 사용하였으며, 말뚝은 탄성 원형 콘크리트로 모델링하였다. 해석 결과, 말뚝의 간격이 넓어짐에 따라 P-multiplier의 값이 leading pile의 영향을 덜 받는 것으로 나타났다. 또한, 단층지반이 다층지반에 비해 수평저항력이 약 4~20% 크게 작용하는 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제21권7호
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• pp.686-696
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• 2011

To deal with pathogens, plants have evolved sophisticated mechanisms including constitutive and induced defense mechanisms. Phytohormones play important roles in plant growth and development, as well as in the systemic response induced by beneficial and pathogen microorganisms. In this work, we identified an Aspergillus ustus isolate that promotes growth and induces developmental changes in Solanum tuberosum and Arabidopsis thaliana. A. ustus inoculation on A. thaliana and S. tuberosum roots induced an increase in shoot and root growth, and lateral root and root hair numbers. Assays performed on Arabidopsis lines to measure reporter gene expression of auxin-induced/ repressed or cell cycle controlled genes (DR5 and CycB1, respectively) showed enhanced GUS activity, when compared with mock-inoculated seedlings. To determine the contribution of phytohormone signaling pathways in the effect elicited by A. ustus, we evaluated the response of a collection of hormone mutants of Arabidopsis defective in auxin, ethylene, cytokinin, or abscisic acid signaling to the inoculation with this fungus. All mutant lines inoculated with A. ustus showed increased biomass production, suggesting that these genes are not required to respond to this fungus. Moreover, we demonstrated that A. ustus synthesizes auxins and gibberellins in liquid cultures. In addition, A. ustus induced systemic resistance against the necrotrophic fungus Botrytis cinerea and the hemibiotrophic bacterium Pseudomonas syringae DC3000, probably through the induction of the expression of salicylic acid, jasmonic acid/ethylene, and camalexin defense-related genes in Arabidopsis.

• Journal of Microbiology and Biotechnology
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• 제25권12호
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• pp.2100-2105
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• 2015

Probiotic bacteria must have not only tolerance against bile salt but also no genes for antibiotic resistance. Leuconostoc citreum is a dominant lactic acid bacterium in various fermented foods, but it is not regarded as a probiotic because it lacks bile salt resistance. Therefore, we aimed to construct a bile salt-resistant L. citreum strain by transforming it with a bile salt hydrolase gene (bsh). We obtained the 1,001 bp bsh gene from the chromosomal DNA of Lactobacillus plantarum and subcloned it into the pCB4170 vector under a constitutive P710 promoter. The resulting vector, pCB4170BSH was transformed into L. citreum CB2567 by electroporation, and bile salt-resistant transformants were selected. Upon incubation with glycodeoxycholic acid sodium salt (GDCA), the L. citreum transformants grew and formed colonies, successfully transcribed the bsh gene, and expressed the BSH enzyme. The recombinant strain grew in up to 0.3% (w/v) GDCA, conditions unsuitable for the host strain. In in vitro digestion conditions of 10 mM bile salt, the transformant was over 67.6% viable, whereas only 0.8% of the host strain survived.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.168-168
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• 2017

Drought conditions during cultivation reduce agricultural production yield less than a theoretical maximum yield under normal condition. Plant specific NAC transcription factors in rice are known to play an essential roles in stress resistance transcriptional regulation. In this study, we report the rice (Oryza sativa L japonica) NAM, AFTF and CUC transcription factor OsNAC17, which is predominantly induced by abiotic stress in leaf, was contribute to the drought tolerance mediated reactive oxygen species (ROS) in transgenic rice plants. Constitutive (PGD1) promoter was introduced to overexpress OsNAC17 and produced the transgenic PDG1:OsNAC17. Overexpression of OsNAC17 throughout the whole plant improved drought resistance phenotype at the vegetative stage. Morphological characteristics such as grain yield, grain filling rate, and total grain weight improved by 22~64% over wild type plants under drought conditions during the reproductive stage. The improved drought tolerance in transgenic rice was involved in reducing stomatal density up to 15% than in wild type plants and in increasing reactive oxygen species-scavenging enzyme. DEG profiling experiment identified 119 up-regulated genes by more than twofold (P<0.01). These genes included UDP-glycosyltransferase family protein, similar to 2-alkenal reductase (NADPH-dependent oxireductase), similar to retinol dehydrogenase 12, Lipoxygenase, and NB-ARC domain containing protein related in cell death. Furthermore, OsNAC17 was act as a transcriptional activator, which has an activation domain in C-terminal region. These result demonstrate that the overexpression of OsNAC17 improve drought tolerance by regulating ROS scavenging enzymes and by reducing stomatal density

• 대한임상검사과학회지
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• 제38권1호
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• pp.38-44
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• 2006

A simple and easy modification of AST by disk diffusion was tested for the detection of induced clindamycin resistant Staphylococci and their antimicrobial susceptibility at the same time. The incidence of inducible clindamycin resistant staphylococci in blood culture and their MIC characterization at Seoul National University Hospital was analyzed by an AST contained disk approximation test (D-zone test) and Etest, respectively. Of the total 309 staphylococcal isolates, 139 (45%) isolates presented constitutive resistance to ERY and CLI (ERY-R, CLI-R phenotype), and 59 were ERY-I/R and CLI-S phenotypes. Of the 59 isolates, 19 (32%) isolates were inducible resistant to CLI. The incidence was higher in S. aureus (66.7%) than coagulase-negative staphylococci (CNS, 26.0%). Especially, methicillin-resistant staphylococci (MRSA, 100%; MRCNS, 45.5%) presented higher inducibility than methicillin susceptible (MSSA, 50%; MSCNS, 20%). For most of the inducible clindamycin resistant staphylococci (15 of 19 isolates), their ERY MIC were high (>$128_{\mu}g/mL$) and were methicillin resistant. The remaining 4 isolates were methicillin susceptible and their ERY MIC were of intermediate concentrations ($1-4_{\mu}g/mL$). We concluded that suscetibility testing of staphylococci, especially methicillin resistant, should include the D-zone test.