• 한국레이저가공학회:학술대회논문집
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• 한국레이저가공학회 2006년도 추계학술발표대회 논문집
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• pp.123-127
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• 2006

Hybrid type microscope with a Scanning Confocal Microscope (SCM) and a shear-force Atomic Force Microscope (AFM) is suggested and preliminarily studied. A image of $120{\times}120{\mu}m^2$ is obtained within 1 second by SCM because scan speed of a X-axis and Y-axis are 1kHz and 1Hz, respectively. Shear-force AFM is able to correctly measure the hight and width of sample with a resolution 8nm. However, the scan speed is slow and it is difficult to distinguish a surface composed of different kinds of materials. We have carried out the measurement of total image of a sample by SCM and an exact analysis of each image by shear-force AFM.

• 한국정밀공학회지
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• 제25권11호
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• pp.52-57
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• 2008

We demonstrate the operation of an apparatus that we call the laser scanning confocal microscopy. It is valuable tool of the investigations for imaging process. We measured the thin metal structure through the SCM manufacture. Confocal microscopy offers several advantages including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens than conventional optical microscope. This research is manufactured of scanning confocal microscopy and after measured of metal materials structure.

• 비파괴검사학회지
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• 제21권2호
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• pp.169-176
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• 2001

근대 산업에서 기계 부품들의 정밀도와 표면 마무리 조건은 그 어느 때보다 더 절박하게 요구되고 있는 가운데, 표면 형상의 측정과 이해는 공학 기술자뿐만 아니라 물리학자나 화학자도 논은 관심을 갖게 되었다. 이런 상황에서 광학적 측정 방법은 opto-mechatronics의 이점을 가지고 진동 측정이나 균열, 결함의 검출 등의 분야에서 유용한 측정 방법으로 사용되고 있고, 표면 형상학의 분야에서 다시 한 번 중요한 역할을 할 것으로 기대를 받고 있다. 본 연구에서는 공초점 현미경의 원리에 대해 서술하였고, 기존의 스캔 방식 공초점 현미경보다 한 차원 개선된 3차원 표면형상 측정 시스템을 개발하였다. 그리고, 표면의 기하적인 형상에 대한 정보를 얻고 그 형상을 가시화하기 위해 컴퓨터 시스템과 통합하고 그에 필요한 소프트웨어를 개발하였다.

• 한국동물학회:뉴스레터
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• 제16권1호
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• pp.60-65
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• 1999

• 한국광학회:학술대회논문집
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• 한국광학회 2004년도 제15회 정기총회 및 동계학술발표회
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• pp.58-59
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• 2004

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2009년도 춘계학술대회 논문집
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• pp.301-302
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• 2009

• 한국정보통신학회논문지
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• 제15권12호
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• pp.2697-2704
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• 2011

조직 공학에서는 하이드로 젤 내 3차원 세포 배양 과정에서 세포와 세포간의 공생관계를 관찰하게 되는데, 시간이 경과함에 따라 세포들의 증가나 상호작용, 분화, 사멸되는 과정들을 고배율 공초점 현미경을 이용하여 단층 촬영하고, 촬영된 이미지들로부터 세포의 공생 메커니즘이나 변화 과정을 관찰 및 분석하기 위한 연구가 진행되고 있다. 본 연구에서는 하이드로 젤 내 3차원 세포 배양 과정에서 단층 촬영된 세포 이미지로부터 클러스터 경계선 정보를 추출하기 위한 수준별 잡음 제거 방법과 클러스터 경계선 추출 방법을 제안하였다. 실험을 통해 제안된 방법은 하이드로젤로 인한 좁쌀 모양의 잡음에 뛰어난 잡음 제거 성능을 보여 줄 뿐만 아니라 복잡한 클러스터들에 대해서 도 정확한 클러스터 경계선을 추출함을 보여 주었다.

• 제어로봇시스템학회논문지
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• 제19권11호
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• pp.979-983
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• 2013

The three-dimensional measurement method of confocal systems is a spot scanning method which has a high resolution and good illumination efficiency. However, conventional confocal systems had a weak point in that it has to perform XY axis scanning to achieve FOV (Field of View) vision through spot scanning. There are some methods to improve this problem involving the use of a galvano mirror [1], pin-hole array, etc. Therefore, in this paper we propose a method to improve a parallel mode confocal system using a micro-lens and pin-hole array in a dual microscope configuration. We made an area scan possible by using a combination MLA (Micro Lens Array) and pin-hole array, and used an objective lens to improve the light transmittance and signal-to-noise ratio. Additionally, we made it possible to change the objective lens so that it is possible to select a lens considering the reflection characteristic of the measuring object and proper magnification. We did an experiment using 5X, 2.3X objective lens, and did a calibration of height using a VLSI calibration target.

• Journal of the Optical Society of Korea
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• 제14권1호
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• pp.42-48
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• 2010

We used video-rate Confocal Laser Scanning Microscopy (CLSM) to observe the motion of blood cells in a micro-channel. Video-rate CLSM allowed us to acquire images at the rate of 30 frames per second. The acquired images were used to perform Particle Image Velocimetry (PIV), thus providing the velocity profile of the blood in a micro-channel. While previous confocal microscopy-assisted PIV required exogenous micro/nano particles as the tracing particles, we employed blood cells as tracing particles for the CLSM in the reflection mode, which uses light back-scattered from the sample. The blood flow at various depths of the micro-channel was observed by adjusting the image plane of the microscope. The velocity profile at different depths of the channel was measured. The confocal micro-PIV technique used in the study was able to measure blood velocity up to a few hundreds ${\mu}m/sec$, equivalent to the blood velocity in the capillaries of a live animal. It is expected that the technique presented can be applied for in vivo blood flow measurement in the capillaries of live animals.

• 한국발생생물학회지:발생과생식
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• 제21권3호
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• pp.229-235
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• 2017

In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash-like movement angles were compared between fresh and low-temperature-preserved ($17^{\circ}C$ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from $11.0{\pm}2.3beats/s$ (fresh sperm) to $5.7{\pm}1.8beats/s$ and increased the flagellar bending angle from $19.8^{\circ}{\pm}13.8^{\circ}$ (fresh) to $30.6^{\circ}{\pm}15.6^{\circ}$. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques.