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http://dx.doi.org/10.3807/JOSK.2010.14.1.042

Particle Image Velocimetry of the Blood Flow in a Micro-channel Using the Confocal Laser Scanning Microscope  

Kim, Wi-Han (School of Mechanical Engineering, Kyungpook National University)
Kim, Chan-Il (School of Mechanical Engineering, Kyungpook National University)
Lee, Sang-Won (School of Mechanical Engineering, Kyungpook National University)
Lim, Soo-Hee (School of Mechanical Engineering, Kyungpook National University)
Park, Cheol-Woo (School of Mechanical Engineering, Kyungpook National University)
Lee, Ho (School of Mechanical Engineering, Kyungpook National University)
Park, Min-Kyu (School of Mechanical and Automotive Engineering Technology, Yeungnam College of Science & Technology)
Publication Information
Journal of the Optical Society of Korea / v.14, no.1, 2010 , pp. 42-48 More about this Journal
Abstract
We used video-rate Confocal Laser Scanning Microscopy (CLSM) to observe the motion of blood cells in a micro-channel. Video-rate CLSM allowed us to acquire images at the rate of 30 frames per second. The acquired images were used to perform Particle Image Velocimetry (PIV), thus providing the velocity profile of the blood in a micro-channel. While previous confocal microscopy-assisted PIV required exogenous micro/nano particles as the tracing particles, we employed blood cells as tracing particles for the CLSM in the reflection mode, which uses light back-scattered from the sample. The blood flow at various depths of the micro-channel was observed by adjusting the image plane of the microscope. The velocity profile at different depths of the channel was measured. The confocal micro-PIV technique used in the study was able to measure blood velocity up to a few hundreds ${\mu}m/sec$, equivalent to the blood velocity in the capillaries of a live animal. It is expected that the technique presented can be applied for in vivo blood flow measurement in the capillaries of live animals.
Keywords
Confocal microscopy; Particle image velocimetry; Blood flow;
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Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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