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Establishment of tissue culture and acclimatization method for in vitro mass propagation of Echeveria laui and Echeveria elegans (에케베리아 라우이(Echeveria laui)와 엘레강스(Echeveria elegans)의 대량증식을 위한 조직배양 및 순화 조건 확립)

  • Kim, Youn Hee;Lee, Gee Young;Kim, Hye Hyeong;Lee, Jae Hong;Jung, Jae Hong;Lee, Sang Deok
    • Journal of Plant Biotechnology
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    • v.46 no.1
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    • pp.22-31
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    • 2019
  • The objective of this study was to investigate the suitable parts for callus induction and optimal concentrations of growth regulators contained in the medium affecting shooting and rooting Echeveria laui and Echeveria elegans for in vitro mass production. To determine the suitable plant parts for callus induction, the leaves were divided into upper, medium and bottom parts and cultured on MS medium at different concentrations with $0{\sim}2mgL^{-1}\;NAA$ and $0{\sim}4 mgL^{-1}BA$. The upper and middle parts of leaves both showed 100% callus formation rate with $NAA\;1\;mgL^{-1}$ and $BA\;1\;mgL^{-1}$ treatment in E. laui. The middle parts of leaves showed 83.3% callus formation rate at $NAA\;2\;mgL^{-1}$ and BA 4 mgL-1 treatment in E. elegans. The shoot induction rate from callus was highest at $NAA\;0.1\;mgL^{-1}$ and $BA\;3\;mgL^{-1}$ treatment in E. laui and $NAA\;0.3\;mgL^{-1}$ in E. elegans. In addition, the number of shoots formation was 10.4 shoots high in $NAA\;1\;mgL^{-1}$ and $BA\;1\;mgL^{-1}$ treatment in E. laui and 12.0 shoots in most effective $NAA\;1\;mgL^{-1}$ and $BA\;0.1\;mgL^{-1}$ treatment in E. elegans. In the case of acclimatization of regenerated plant, growth characteristics did not show any significant difference (35 ~ 55%) shading with respect to the different ratio of substrate mixture, and it was determined that would be appropriate considered plant height and appearance preference of E. laui and E. elegans. It was established that the optimization of culture condition was responsible for the mass propagation in vitro cultures of E. laui and E. elegans.

The Morphologic Characteristics of Step-pool Structures in a Steep Mountain Stream, Chuncheon, Gangwon-do (강원도 춘천시 근교의 산지계류에 형성된 계단상 하상구조의 특징)

  • Kim, Suk Woo;Chun, Kun Woo;Park, Chong Min;Nam, Soo Youn;Lim, Young Hyup;Kim, Young Seol
    • Journal of Korean Society of Forest Science
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    • v.100 no.2
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    • pp.202-211
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    • 2011
  • The geometric characteristics of step-pool structures and how they are influenced by channel characteristics were investigated in a steep mountain stream in the Experimental Forests of Kangwon National University in Chuncheon, Gangwon-do. Average values of steps for the study reaches were as follows: step spacing, 4.69 m; step height, 0.47 m; step drop, 0.71 m; step-forming particle sizes, 0.68 m; number, 21steps/ 100 m; the ratio of step spacing to channel width, 0.5; and step steepness, 0.13. Relationships between spacing and height of steps and channel gradient showed a negative- and positive correlation, respectively, whereas all geometric variables of steps manifested poor correlation with channel width. Therefore, step steepness, expressed as the ratio of step height to step spacing, increased as channel gradient increased. The ratio of step steepness to channel gradient representing the criterion of maximum flow resistance was 1.2, indicating the channel bed's stable condition. In particular, the relationship between the ratio of step drop to step height and channel gradient showed a significant negative correlation, suggesting the influence of step-pool geometry in trapping sediment and providing an aquatic habitat. Positive correlations also exist between spacing and drop of steps and step particles. Our findings suggest that the dynamics of step-pool structures may strongly control physical and ecological environments in steep mountain streams, so understanding them is essential for stream management.

An Analysis of the Managerial Level's Gender Gap and "Glass Ceiling" of the Corporation (기업 관리직의 젠더 격차와 "유리천장" 분석)

  • Cho, Heawon;Hahm, Inhee
    • 한국사회정책
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    • v.23 no.2
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    • pp.49-81
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    • 2016
  • This study agrees with the idea that a situation centered perspective provides a useful contribution in understanding women's attitude on organizations. Women's occupational experiences are less related to their "femaleness" than to the structural constraints inherent in the occupational positions women fill. So characteristics of the organizational situation including gender composition and hierarchical status may "shape and define" women's experience on the job. The present study examined the managerial level's gender gap and "glass ceiling" of the corporation. According to Kanter, if the ratio of women to men in organizations begins to shift, as affirmative action and new hiring and promotion policies promised, forms of relationships and corporate culture should also change. However, the mere presence of women on workplace may not, in itself, result in women-friendly work condition. This study analyzes "Korean Women Manger Panel survey(2010 3rd. wave)" to examine how much gender gap of the managerial level persists and when the glass ceiling effect emerges. Using t-test and ANOVA, various aspects of the gender gap within managerial level were verified. The most significant finding is the glass ceiling effect starts from very low level of management. Policy implications from the statistical analysis of the Panel survey are: 1) We need to increase the absolute number of the women managers for securing middle level women leadership pipe line. 2) We need to confront the fact that the glass ceiling starts from the very low managerial level, and to explore more realistic way to break up the vicious circle for the tokenism. and 3) We need to looking beyond numbers in approaching women's matter at work. At the cultural and institutional level, work-family programs and policies, women's ratings of their competence, and family-friendly organization's climate should be considered.

A Study on the Structural Reinforcement of the Modified Caisson Floating Dock (개조된 케이슨 플로팅 도크의 구조 보강에 대한 연구)

  • Kim, Hong-Jo;Seo, Kwang-Cheol;Park, Joo-Shin
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.1
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    • pp.172-178
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    • 2021
  • In the ship repair market, interest in maintenance and repair is steadily increasing due to the reinforcement of prevention of environmental pollution caused by ships and the reinforcement of safety standards for ship structures. By reflecting this effect, the number of requests for repairs by foreign shipping companies increases to repair shipbuilders in the Southwest Sea. However, because most of the repair shipbuilders in the southwestern area are small and medium-sized companies, it is difficult to lead to the integrated synergy effect of the repair shipbuilding companies. Moreover, the infrastructure is not integrated; hence, using the infrastructure jointly is a challenge, which acts as an obstacle to the activation of the repair shipbuilding industry. Floating docks are indispensable to operating the repair shipbuilding business; in addition, most of them are operated through renovation/repair after importing aging caisson docks from overseas. However, their service life is more than 30 years; additionally, there is no structure inspection standard. Therefore, it is vulnerable to the safety field. In this study, the finite element analysis program of ANSYS was used to evaluate the structural safety of the modified caisson dock and obtain additional structural reinforcement schemes to solve the derived problems. For the floating docks, there are classification regulations; however, concerning structural strength, the regulations are insufficient, and the applicability is inferior. These insufficient evaluation areas were supplemented through a detailed structural FE-analysis. The reinforcement plan was decided by reinforcing the pontoon deck and reinforcement of the side tank, considering the characteristics of the repair shipyard condition. The final plan was selected to reinforce the side wing tank through the structural analysis of the decision; in addition, the actual structure was fabricated to reflect the reinforcement plan. Our results can be used as reference data for improving the structural strength of similar facilities; we believe that the optimal solution can be found quickly if this method is used during renovation/repair.

Effect of Growth Temperature and MA Storage on Quality and Storability of Red Romaine Baby Leaves (생육온도와 MA저장이 적로메인 상추 어린잎의 품질과 저장성에 미치는 영향)

  • Choi, Dam Hee;Lee, Joo Hwan;Choi, In-Lee;Kang, Ho-Min
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.27 no.3
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    • pp.187-192
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    • 2021
  • This study was conducted to compare the quality of baby leaves grown under several temperature conditions and the storage properties of MA storage for romaine lettuce. It was grown for 5 weeks under an artificial light source (200 µmol·m-2·s-1) in a chamber at 21℃, 28℃, and 35℃. The growth and quality of red romaine lettuce that grown in different temperatures were investigated at the end of cultivation, and the oxygen, carbon dioxide, and ethylene concentrations in the 20,000 cc OTR film and perforated film packed with lettuces were measured for 36 and 12 days, respectively. The red romaine lettuce baby leaf was examined for color, chlorophyll, and visual quality at the end of storage. The maximum quantum yield of baby leaf grown in different temperatures at 7days before the harvest was higher at 21℃ and 28℃ growth temperature treatments. On harvest day, the leaf length measured was longest at 28℃, and the leaf width was wider at 21℃ and 28℃, and the number of leaves was similar to 5-6 at all cultivation temperatures. Leaf weight, root weight, and dry weight were found to be higher at 21℃, and tended to decrease as the cultivation temperature increased. The concentration of ethylene in the film of the MA storage treatments was maintained at 1~2 µL·L-1 until the end of storage in all treatments regardless of the cultivation temperature. Oxygen concentration in the MA treatment used 20,000 OTR film was maintained at around 19.5%, and carbon dioxide concentration around 1% that was satisfied the CA conditions. Both Hunter a* and b* values were generally higher in the MA storage treatment at the end of storage day. The chlorophyll content was decreased as the cultivation temperature increased, and was lower in the MA storage treatment than in the perforated film treatment. Visual quality was 3 points or higher in the MA storage treatment at 21℃ growth treatment, and it was maintained marketability. As the above results, the growth of baby leaves of romaine lettuce was the best at 21℃ treatment, and the lower the cultivation temperature, the longer the shelf life. And it was possible to extend the shelf life by 3 times by showing excellent visual quality at the MA storage treatment that satisfies the carbon dioxide concentration of CA condition until the end of storage day.

Identification of LED Lights for the Attraction of Bemisia Tabaci and Effect of Host Plant in the Initial Periods (담배가루이 유인용 LED 선발과 기주식물이 초기 유인력에 미치는 영향)

  • Kwon, D.H.;Kwon, M.J.;Yang, D.Y.;Ahn, Y.K.;Hong, K.H.;Park, M.R.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.22 no.2
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    • pp.123-133
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    • 2020
  • Four LEDs (blue, green, red, and white light) were tested to identify the most attractive wave length to utilize as the forecasting tools for the B. tabaci in glass houses. Attractiveness was evaluated by the total number of the B. tabaci attached to a yellow sticky trap. In the condition of no host plant supplement, the attraction efficacy was ordered from high to low as blue light (107.3±2.5), white light (83.0±12.1), red light (58±21.8), and green light (39.7±8.1). In the supplement of the host plant, the attraction was observed in the order of blue light (52±17.4), red light (38.7±5.8), green light (12.7±1.5), and white light (11.7±5.0). In both experimental conditions, blue light showed the highest attraction. In terms of the host plant effect to LED attraction, it varied following as white light (85.9%), green light (68.1%), blue light (51.6%), and red light (33.3%). This result suggests that red light is the least affected by the host plant. In the evaluation of the relative control efficacy, it was determined following as red light (66.7%), blue light (48.5%), green light (31.9%) and white light (14.1%) (F3,8 = 14.7, P = 0.001). Taken together, blue light had a very high initial attraction, and red light was revealed low attraction effect by the supplement of the host plant. In field demonstration experiments, a high attractive efficacy was not observed due to low-temperature conditions, but similar higher attractive efficacy was observed in blue and red lights compared to the control. The commercialization of LEDs using red and blue in the future is expected to provide important information regarding B. tabaci population density forecast in glass house.

Analysis of Local Government Environmental Impact Assessment (EIA) Ordinances and Preparation of Consultation Guidelinesfor EIA - A Case of Incheon Metropolitan City - (지방자치단체 환경영향평가 조례 현황 분석과 협의 지침서 작성 방안 - 인천광역시 사례를 중심으로 -)

  • Lee, Jongook;Cho, Kyeong Doo
    • Journal of Environmental Impact Assessment
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    • v.31 no.4
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    • pp.226-240
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    • 2022
  • Local governments over a certain size in Republic of Korea may conduct Environmental Impact Assessment (EIA) considering regional characteristics if it is necessary, in accordance with Article 42 of the 「Environmental Impact Assessment Act」. However, it was investigated that the number of local government EIA operation in many local governments was less than initial expectations. In order to improve it, the status of ordinances and consultation guidelines which are different for each local government need to be compared, and the institutional issues forthe relevant local governments must be found considering regional characteristics. Furthermore, detailed regulation and guidance on the local government EIA procedure should be included in the consultation guidelines and related information need to be provided. In this study, focusing on the case of Incheon Metropolitan City, the status of local government EIA ordinances in metropolitan cities and provinces with a similar condition was investigated, and the types and scope of target projects were compared and analyzed. In addition, consultation guidelines forIncheon Metropolitan City were written, and improvements on the procedure flow and overall schedule designation derived from the process were presented. In the case of Incheon Metropolitan City, there were no detailed information officially announced regarding the regulations of the local government EIA ordinance and follow-up management, so the administrative system of the local government needed to be reinforced in this field. Meanwhile, considering the status of local environment and geography, some target project types were deemed necessary to be added: port construction projects, water resource development projects, railroad construction projects, and military facilities installation projects. The results of this study will provide useful information to local governments which want to improve their operation effectiveness by reorganizing the local government EIA system and preparing specific guidelines.

Ventilation at Supra-Optimal Temperature Leading High Relative Humidity Controls Powdery Mildew, Silverleaf Whitefly, Mite and Inhibits the Flowering of Korean Melon in a Greenhouse Cultivation (참외 시설 재배 시 고온에서의 환기 처리에 의한 상대습도 상승과 흰가루병, 담배가루이, 응애 방제 및 개화 억제)

  • Seo, Tae Cheol;Kim, Jin Hyun;Kim, Seung Yu;Cho, Myeong Whan;Choi, Man Kwon;Ryu, Hee Ryong;Shin, Hyun Ho;Lee, Choung Keun
    • Journal of Bio-Environment Control
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    • v.31 no.1
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    • pp.43-51
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    • 2022
  • This study was conducted to investigate the effect of ventilation at high temperature on the control of powdery mildew, silverleaf whitefly two-spotted spider mite occurred at Korean melon cultivation greenhouse, and on leaf rolling and flowering of the plant in summer season. 'Alchanggul' grafted onto 'Hidden Power' rootstock was planted on soil bed with the distance of 40 cm. Three ventilation temperatures of 45℃, 40℃, and 35℃ as set points were compared. Ventilation treatment was done by control of side window operation from 18th June to 13th July when silverleaf whitefly, mite, and powdery mildew were occurred in all greenhouses. The temperature inside greenhouse was increased up to the set temperature point on sunny days and maintained for about 9 hours with high relative humidity at 45℃ condition. The differences of day maximum air temperature and day minimum RH were the highest at 45℃ treatment. After 11 days of treatments, the damage by powdery mildew and two-spotted spider mite was almost recovered at 45℃ treatment but not at 40 and 35℃. The population of silverleaf whitefly and two-spotted spider mite were significantly decreased at 45℃ treatment at 14 days after treatment, while powdery mildew symptom was not significantly decreased. Leaf rolling was observed at high temperature but not severe at 45℃ treatment. After 26 days of treatments, female flowers did not bloom at all at 45℃ treatment, and the number of male flowers was 1.2 among 15 nodes of newly grown shoots. As the result, it indicates that ventilation at the high temperature of 45℃ for about 2 to 3 weeks can be an applicable method to control above mentioned pests and disease, and to recover the vegetative growth of Korean melon by reducing flowering of the plant.

A Characteristics and Management Plan of Elaeocarpus sylvestris var. ellipticus Population Distributed in Munseom(island), a Natural Monument (천연기념물 문섬의 담팔수 개체군 특성 및 관리방안)

  • Choi, Byoung-Ki;Lee, Ho-Sang;Seo, Yeon-Ok;Choi, Hyung-Soon;Yang, Ju-Eun;Song, Kuk-Man;Song, Gwan-Pil
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.39 no.1
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    • pp.37-42
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    • 2021
  • Munseom (Island) is known as the largest native Elaeocarpus sylvestris var. ellipticus in Korea. Recently, disease damage from the E. sylvestris var. ellipticus community has been reported in Munseom. This study was conducted to understand the damage situation and growth characteristics of the E. sylvestris var. ellipticus population in Munseom. The purpose of this study was to evaluate the spontaneous regeneration potential of the community by grasping the characteristics of the population of surviving E. sylvestris var. ellipticus individuals, and to discuss ways to restore native habitats. A total population survey was conducted to determine the distribution status of E. sylvestris var. ellipticus individuals. The field survey carefully recorded the height of tree, DBH, DRH, and growing status, along with GPS location information of the individual. The growth status of E. sylvestris var. ellipticus individuals distributed in Munseom and the characteristics of tree height, DBH, DRH of each individual were analyzed. The total number of E. sylvestris var. ellipticus populations identified in Munseom was 293. The dense E. sylvestris var. ellipticus community reported in 2005 has been identified as being greatly damaged or damaged. In particular, the damage was more serious in mature trees with a height of 6m or more forming the canopy layer in the forest, and it was confirmed that 80.6% of the mature trees died. In the growth characteristics of the old-growth tree, which is an indicator of the health of the forest based on DRH, 75.4% died or the growth condition was found to be poor. In order to restore the native habitat of E. sylvestris var. ellipticus in Munseom, conservation efforts such as pest control, preservation of mature trees, control of the territory of young trees, and reintroduction after ex-situ transplantation etc. are considered necessary.

Comparative Analysis of Oral Health Awareness, Knowledge and Behavior according to the Major of Some Female Students for Life Care (일부 여학생들의 라이프케어를 위한 전공별 구강보건 인식, 지식 및 행태 비교분석)

  • Lee, Mi-Lim;Lee, Hyo-Cheol
    • Journal of Korea Entertainment Industry Association
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    • v.14 no.1
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    • pp.149-158
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    • 2020
  • The purpose of this study was to examine relation of oral health awareness, knowledge and behavior in department dental hygiene, health-related majors and health-unrelated majors in attempt to provide basic data of oral health promotion and oral health education. This study were 591 female H university students in Gwangju. The interests of oral health, the recognition of importance for oral health, and recognition of one's own oral health status were highest in dental hygiene students, followed by health-unrelated majors and health-related majors. The level of oral health knowledge by the major was highest in dental hygiene students(9.73), followed by health-related majors(9.14) and health-unrelated majors(9.05). In the oral health behavior by major, students who brushed more than three times a day, used the oral care products and received regular dental examinations within 1 year were the highest in the dental hygiene major, followed by health-related majors and health-unrelated majors. The experience of scaling within 1 year was the highest in the dental hygiene major, followed by health-related majors and health-unrelated majors. The higher the oral health knowledge, increased awareness of oral health concern and the importance of oral health. Also, the higher the degree of interest in oral health, the greater the recognition that oral health is important and the more the oral health condition is perceived as healthy, It was found that the number of brushing increased. In this results, the higher the oral health knowledge, the higher the oral health awareness and the oral health behavior. It is necessary to find ways to develop or utilize various oral health education for university students.