• Journal of Life Science
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• v.28 no.3
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• pp.293-299
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• 2018

Viticis fructus (fruits of Vitex rotundifolia) is the dried fruit from Vitex rotundifolia; is a traditional medicine for treating inflammation, migraines, chronic bronchitis, headaches, eye pain, and gastrointestinal infections; and demonstrates various bioactivities, including anti-allergic, anti-cancer, and anti-inflammatory effects, which are partly due to its phenolic compound content. This study examines the inhibitory effects of viticis fructus (fruits of Vitex rotundifolia) on MMP-2 and MMP-9 expression using gelatin zymography and RT-PCR in phorbol-12-myristate-13-acetate (PMA)-induced HT-1080 fibro-sarcoma cells. Fruits of Vitex rotundifolia were extracted twice using dichloromethane ($CH_2Cl_2$) and methanol (MeOH). The combined crude extracts ($CH_2Cl_2$ and MeOH) significantly inhibited MMP-2 and MMP-9 activities in gelatin zymography. The combined extracts were fractionated into n-hexane, 85% aqueous methanol (85% aq. MeOH), n-butanol, and water, successively according to polarity. Among all solvent-partitioned fractions, 85% aq. MeOH fractions showed the strongest inhibition on the activation of MMP-2 and MMP-9 in gelatin zymography. In PMA-stimulated HT-1080 cells, the expression levels of MMP-2 and MMP-9 mRNA were also greatly inhibited by the 85% aq. MeOH fraction. These results suggest that viticis fructus can be used as an excellent source for anti-invasive agents.

• Journal of Pharmacopuncture
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• v.19 no.2
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• pp.137-144
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• 2016

Objectives: Several studies have revealed that systemic hypertension is strongly associated with cataractogenesis. However, the pathophysiology and treatment is often unclear. In this study, we evaluated the anti-cataractogenic effect of cinnamaldehyde (CA), a natural organic compound, in rats with fructose-induced hypertension. Methods: The rats were divided into six groups. For six weeks, the normal group received a suspension of 0.5% carboxy methyl cellulose (10 mL/kg/day, p.o.) while five other groups received a 10% (w/v) fructose solution in their drinking water to induce hypertension. By the end of the third week hypertension had been induced in all the animals receiving fructose. From the beginning of the fourth week to the end of the sixth week, one of those five groups (control) continued to receive only 10% (w/v) fructose solution, one group (standard) received ramipril (1 mg/kg/day, p.o.) plus 10% (w/v) fructose solution, and three groups (experimental) received CA at doses of 20, 30, and 40 mg/kg/day p.o., plus 10% (w/v) fructose solution. Blood pressure was measured weekly using a non-invasive blood pressure apparatus. After six weeks, the animals were sacrificed, and the anti-cataractogenic effects on the eye lenses were evaluated. Results: Administration of fructose elevated both the systolic and the diastolic blood pressures, which were significantly reduced by CA at all dose levels. In the control group, a significant increase in the malonaldehyde (MDA) level and decreases in the total protein, $Ca^{2+}$adenosine triphosphate (ATP)ase activity, glutathione peroxidase, catalase, superoxide dismutase and glutathione levels, as compared to the normal group, were observed. Administration of CA at all doses significantly restored the enzymatic, non-enzymatic, antioxidants, total protein, and $Ca^{2+}$ATPase levels, but decreased the MDA level, as compared to the control group. Conclusion: The present study revealed that CA modulated the antioxidant parameters of the serum and lens homogenates in hypertension-induced cataractogenic animals.

• Journal of Korean Ophthalmic Optics Society
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• v.4 no.2
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• pp.129-133
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• 1999

For the myopia eyes of the people which are ametropia, the classified distribution has showed % for the simple myopic, 50% for the compound myopic astigmatism, 15% for the simple myopic astigmatism and 19% for the mixed astigmatism. The myopic ametropia for the both eyes has the distribution of 35% for -0.50D~-2.00Dptr, 54% for -2.00~6.00Dptr, and 11% for over -6.00Dptr. The classifying distribution for the age for the myopic ametropia was 54% for 15~20, 22% for 21~40, 14% for 41~60 and 10% for 61. The occupational distribution for the myopic ametropia has showed 61.5% for the student(Middle, High, College), 13.5% for the office worker, and 15% for the house wives as well as the small business. The hour affecting the refraction most for a day was after P.M. 7 which recorded 45% as the highest value. The reason is that the myopia degree decreases in the morning as the cornea flats and the situation is reversed in the afternoon so that there is a difference of Sph -0.50D and as getting darker the refraction degree of the light coming through the enlarged pupil refraction around the cornea is high. For the seasons the highest myopic degree has been recorded for 68% in the summer due to the shortage of nutrition and the climination inside the body by the exhaustion of sweat. In the blood types A and B are distributed closely as 34% and more active man with O has recorded higher myopic degree than woman. However woman showed higher accommodation power than man regardless the blood types. In the characteristic factors of myopic eye, the character feels fatigue easily has showed the distribution for 42% which is the highest and it could be classified largely by two the.

• Journal of Aquaculture
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• v.11 no.3
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• pp.337-344
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• 1998

This paper documented mophological changes of embryonic development and first zoea larvae of snow crab, Chinoecetes opilio. Female crabs were sampled by the Danish seine fishery at the depth about 200m in Sep. 1997 in the eastern coast of Korea. Female with newly berried eggs was reared at the water temperature of 5$^{\circ}C$ till the time of hatching. The results obtained are as follwos. Embryonic development : According to morphogenesis of fertilized eggs, the developemental process of the embryo was classified into the following seven stages : First stage (cleavage and blastula stage, 24 days) Second stage (gastrula stage, 72 days) Third stage (nauplius stage, 22 days) Fourth stage (metanauplius stage, 57days) Fifth stage (stage of a pigmentary deposit in the compound eye, 30 days) sixth stage (chromatophore appearance stage in maxillipede, 56 days) Seventh stage (hatching stage, 36 days) Larvae hatched as prezoeas and they molted to first zoea in about an hour. The first zoea is 4.6 to 5.1mm in length, 3.2~3.6mm in width. The abdomen consists of five segments and a bifurcate telson.

• The Korean Journal of Zoology
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• v.15 no.3
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• pp.111-131
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• 1972

Fine structures of retina of an ommatidium in dragonfly, having eyes of closed rhabdom type, were studied under light and electron microscopes. The ommatidia consisted of eight retinular cells distributed in a circular pattern and the retinular layer in turn can be divided into three sublayers according to the number of cells in the retina. Each retinular cell has different starting points in the retina and the length of retinular cells is varied greatly; the length of one distal retinular cell shows one half of that of others. In the middle layer, three proximal retinular cells interconnect the adjacent two rhabdoms which are triangular in the appearence of the cross section which in turn consisted of tubular, parallel and lamellated microvilli. The rhabdom is formed by three rhabdomeres, each of which is separated by $120^{\circ}$ between them, but they can be distinguished into two parts according to electron density. Around the outer part of microvilli composing rhabdom, electron density was much less than the inner part of the structure. The microvilli of the inner part appear to be connected to the cytoplasm of retinular cells. Rough endoplsmic reticulum with enlarged cisternae runs through the vacuoles in the outer part of distal retinular cells. Abundant mitochondria concentrated in the vicinity of rhabdom are found at the central part of the retinular cells, while in the area of immediate vicinity of the rhabdom, prominent vacuoles are observed. Above the rhabdom of an ommtidium stands a crystalline cone which is consisted of four cone cells arranged radially along the axis. The crystalline cone is surrounded by cells containing pigment granules. The outermost photoreceptor element of an ommatidium is corneal lens.

• Journal of Dairy Science and Biotechnology
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• v.34 no.2
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• pp.117-135
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• 2016

The present study was performed to develop a functional raw food material from hydrolyzed whey protein powder (23%-GNANA) medication containing sialic acid as a marker compound that is naturally occurring at 7% concentration in GMP (glycomacropeptide). GMP is used worldwide in foodstuffs for babies and infants and is obtained from the milk protein as safe food. While the purpose of our detailed evaluation was aimed to assess preliminary NOAEL values for and above 2,000 mg/kg/day, a clinical dose allowance for 23%-GNANA (as per characteristic of a functional health product, a highly refined test substance of 23% (v/v) sialic acid combined in GMP), at the same time we also wanted to assess the safety of GMP hydrolyzate lacking sialic acid but with identical properties as GMP. Animal safety evaluation was conducted using 23%-GNANA as the test substance, produced from hydrolyzed whey protein powder (product name: HELICOBACTROL-23; provided by Medinutrol Inc. [Korea]; composed of 23% sialic acid and GMP protein) after isolating the sialic acid using enzymes approved as food additives, with GMP as a raw material, and subsequently increasing the content of xx up to 23% through 80% (v/v) ethanol soaking and concentrating, in accordance with GLP Guideline. The animal safety evaluation mentioned above was made on the basis of toxicity in SPF Sprague-Dawley female and male rats dosed with 10 mL of the test substance diluted to 0, 1,250, 2,500, and 5,000 mg/kg directly into their stomachs for 90 d. This was determined in terms of the general symptoms and animal viability, weight and amount of feed intake, eye examination, uracrasia tests, hematological and blood biochemical disorder tests, blood coagulation test, abnormal intestine weight, abnormalities during postmortem and histopathological examinations. Statistical significance was set at P<0.05. Based on the toxicity determination, a certain minor effect associated with the test substance was observed in male rats with no major effects of the tested substance, in comparison with the control group dosed with sterilized water. Nevertheless, the NOAEL value, evaluated as per toxicity criteria, was verified as 5,000 mg/kg/day (P<0.05). Similarly, for female rats, a certain minor effect associated with the test substance was observed in 5,000 mg/kg/day dosed group, with no major effect, yet the NOAEL value (as assessed as per toxicity criteria) was determined to be 5,000 mg/kg/day (P<0.05), which was the same as for male rats. Accordingly, the NOAEL values of the test substances for all female and male rats were finally verified as 5,000 mg/kg/day (P<0.05). In conclusion, it was determined that the 23%-GNANA test substance exceeds 2,000 mg/kg/day, the clinical allowance characteristic for functional health food, and was finally evaluated to cause no safety concerns when used as a raw material in functional health food production, which was the ultimate goal of the present study.

• Journal of Bio-Environment Control
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• v.25 no.1
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• pp.16-23
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• 2016

This study was carried out to investigate the effects of artificial light sources on growth and yield of hydroponically grown Peucedanum japonicum in plant factory. Treatments were composed with; florescent lamp(FL) as control, and LED lights; R:B(2:1, RB), R:B:W(2:1:3, RBW), and R:B:G:W(2:1:0.5:3, RBGW). Plant height of RBGW and FL treatments were superior to RB and RBW. Leaf weight of RBW and RB were superior to FL and RBGW. There were no significant difference of leaf length and thickness among the treatments. Lightness of leaves was same tendency with plant height. Total phenolic compound content was the high in order of RB as $105.77mg{\cdot}100g^{-1}$ GE, RBW as $92.52mg{\cdot}100g^{-1}$ GE, FL as $89.08mg{\cdot}100g^{-1}$ GE, and RBGW $82.00mg{\cdot}100g^{-1}$ GE. Total flavonoids were not detected in all treatments. Vitamin C content was the highest in RB and the lowest in FL. Total dietary fiber were the highest in FL and the lowest in RBGW. There was no significant difference cystein and methionine contents among the treatments. Concludely, yield, total phenolic compounds, and vitamin C content was high in RBW and RB. We reached conclusion that RBW is best artificial light source considering yield, functionality and eye fatigability when work. We recommend to further study LED pulse and duty rates for increasing functionality.

• Journal of Aquaculture
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• v.6 no.4
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• pp.235-253
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• 1993

The freshwater crab, Eriocheir japonicus inhabits from sub-tropical to temperate zone in Asia. This species belongs to a large size group among freshwater crabs. Common size of this crab is 5-6cm in carapace length and occasionally 7cm in carapace length. This species of crab used to inhabit in estuaries, rivers and inland waters in Korea. However, natural population recently has been rapidly decreased because of pollution and lost their habitats by suburban development. Therefore, development of proper methods of seedling production to increase natural stock became necessity. As parts of achieving this goal, duration from mating to spawning, egg incubation period, and egg development of this species were studied. The influence of temperatures and salinities on the egg incubation and hatching was also investigated. It took 2-8 hours from mating to egg spawning and the spawning lasted 3-9 hours from the first spawning. Egg numbers per female (6cm in carapace length) were 380,000­410,000. Optimum temperature for egg incubation was $17\~23^{\circ}C$ and optimum salinity, $14.0\~31.5\%o$. Incubation period of the eggs at $14^{\circ}C,\;17^{\circ}C,\;20^{\circ}C,\;26^{\circ}C,\;and\;28^{\circ}C$ was 42, 28, 21, 15, and 14 days. respectively. Relation between temperature (X) and incubation days (Y) was LogY = Log 2764.267 - 1.608 LogX. A female can spawn 4-6 times per year by manipulation of environmental conditions. Under the conditions of $18^{\circ}C\;and\;24.5\%o$, it took 6 days up to embryo formation, 18 days up to compound eye formation, 22 days up to abdominal movement, and 25 days up to hatch out as zoea larvae.