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http://dx.doi.org/10.5352/JLS.2018.28.3.293

Anti-invasive Effect of the Solvent-partitioned Fractions from Viticis Fructus in PMA-induced HT-1080 Cells  

Son, Jaemin (Ocean Science & Technology School, Korea Maritime & Ocean University)
Kim, Hojun (Division of Marine Bioscience, Korea Maritime & Ocean University)
Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University)
Seo, Youngwan (Ocean Science & Technology School, Korea Maritime & Ocean University)
Publication Information
Journal of Life Science / v.28, no.3, 2018 , pp. 293-299 More about this Journal
Abstract
Viticis fructus (fruits of Vitex rotundifolia) is the dried fruit from Vitex rotundifolia; is a traditional medicine for treating inflammation, migraines, chronic bronchitis, headaches, eye pain, and gastrointestinal infections; and demonstrates various bioactivities, including anti-allergic, anti-cancer, and anti-inflammatory effects, which are partly due to its phenolic compound content. This study examines the inhibitory effects of viticis fructus (fruits of Vitex rotundifolia) on MMP-2 and MMP-9 expression using gelatin zymography and RT-PCR in phorbol-12-myristate-13-acetate (PMA)-induced HT-1080 fibro-sarcoma cells. Fruits of Vitex rotundifolia were extracted twice using dichloromethane ($CH_2Cl_2$) and methanol (MeOH). The combined crude extracts ($CH_2Cl_2$ and MeOH) significantly inhibited MMP-2 and MMP-9 activities in gelatin zymography. The combined extracts were fractionated into n-hexane, 85% aqueous methanol (85% aq. MeOH), n-butanol, and water, successively according to polarity. Among all solvent-partitioned fractions, 85% aq. MeOH fractions showed the strongest inhibition on the activation of MMP-2 and MMP-9 in gelatin zymography. In PMA-stimulated HT-1080 cells, the expression levels of MMP-2 and MMP-9 mRNA were also greatly inhibited by the 85% aq. MeOH fraction. These results suggest that viticis fructus can be used as an excellent source for anti-invasive agents.
Keywords
Anti-invasive; gelatin zymography; HT-1080; MMP inhibition; Vitex rotundifolia fructus;
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