• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.1
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• pp.140-151
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• 2001

This study based on the theoretical understanding of multi-layer planting which have engineering, ecological and landscape benefits, was conducted to find out the status of multi-layer planting in the apartment and neighborhood park in Seoul. This study was also aimed to seek for the problematic matters, and suggest a solution on the current multi-layer planting. The results of this study were as follows; 1) Since landscape woody plants have been classified just as tree and shrub in Korea, the classification for the multi-layer planting has been unreasonable, and landscape woody plants might have been classified as tree, sub-tree and shrub, or upper, middle, and lower-layer, It could be defined that upper layer is over eight meters in full growth, middle over 3-8 meters and lower under 3 meters. 2) In apartments, the upper layer consisted of eighteen species, the middle and lower layer seven species each. In neighborhood parks, the upper layer consisted of fifteen species, and the middle and lower layer five species each. 3) In terms of planting year of the surveyed areas, there were no differences in the number of species when planting year of the apartment was divided into two groups, the first half(1900-1995) and the second(1996-2000). But, in terms of individual occupation, the percentage was decreased in upper layer, while there was increasing in middle and lower layer. 4) As the result of survey of multi-layered area, it appeared that apartment was shown 0.65 percent and neighborhood park 0.61 percent of the planted area, which was less than 1 percentage of landscape architecturally planted area. 5) In apartment, the number of individual in middle layers has been increased in the first half and the second, but with respect to the correlation with multi-layered area, the apartments had the "$\rho$=0.208", saying that increasing middle layer was scattered planting instead of multi-layered planting. 6) In planting at the apartments in Korea, the planting density was limited, because the layer division was restricted to only tree and shrub. On the contrary, it was divided into upper, middle and lower tree in Japan. Therefore, in Korea, it should be classified as the planting density by dividing into tree, sub-tree, and shrubs, or upper, middle and lower tree by the law. And, it should be considered that the multi-layered planting has a proper organic relation as well as the planting density.g density.

• The KIPS Transactions:PartA
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• v.19A no.1
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• pp.1-8
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• 2012

Network-based intelligent robot is connected with network system, provides interactions with humans, and carries out its own roles on ubiquitous computing environments. URC (Ubiquitous Robot Companion) robot has been proposed to develop network-based robot by applying distributed computing techniques. On URC robot, it is possible to save the computing power of robot client by environments, has been proposed to develop robot software using service-oriented architecture on server-client computing environments. The SOMAR client robot consists of two layers - device service layer and robot service layer. The device service controls physical devices, and the robot service abstracts robot's services, which are newly defined and generated by combining many device services. RSEL (Robot Service Executing Language) is defined in this paper to represent relations and connections between device services and robot services. A RESL document, including robot services by combining several device services, is translated to a programming language for robot client system using RSEL translator, then the translated source program is compiled and uploaded to robot client system with RPC (Remote Procedure Call) command. A SOMAR client system is easy to be applied to embedded systems of host/target architecture. Moreover it is possible to produce a light-weight URC client robot by reducing workload of RSEL processing engine.

• Journal of Embryo Transfer
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• v.30 no.3
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• pp.239-248
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• 2015

The nature of molecular mechanisms governing embryonic cell block is largely unknown, but recent reports have demonstrated that proper execution of programmed cell death is crucial for this process. The main objective of this study is to determine effects of programmed cell death on porcine oocytes development in vitro after parthenogenesis. Among the blastocysts matured in 3MA, MAP1LC3A and ATG5 RNA gene expression level increased in the order of Cyst < 3MA < RP. However, Casp-3 and TNF-r RNA gene expression level decreased in the order of RP < 3MA < Cyst. Expression of mTOR within the RP-cultured blastocyst was the most highly to the inner cell mass, while 3MA-cultured blastocyst showed very lowest expression in inner cell mass. The expression of mTOR showed a pattern opposite to that of MAP1LC3A. That is, its expression was the lowest in Cyst group. When the enzymatic activity of MMP-2 and MMP-9 was assessed in culture, the level of active MMP-9 was higher expression in the medium of each RP treatment group, with the level of another treatment group being relatively higher. Analyses of TIMP-2 and TIMP-3 revealed that their expression was higher in groups that did not receive RP treatment. More specifically, the level of TIMP-2 was not affected by Cyst treatment, while the level of TIMP-3 was higher in 3MA and RP treatment group. There was highly cell division activation efficiency of parthenogenesis on cultured system of RP supplement IVC medium. Therefore, these results suggest that embryo development was significantly increased in conditional culture medium with active autophagy as compared to common cultured condition. Further investigation of this distinction may enable the development of innovative improvements for the production of porcine somatic cell nuclear transfer.

• Journal of Animal Science and Technology
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• v.54 no.4
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• pp.283-290
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• 2012

Cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. The objective of this study was to investigate the effect of taurine, hypotaurine and trehalose as antioxidants on the function of the freezing-thawed sperm in Korean Jeju Black Bull. The semen was cryopreserved with tris egg yolk extendercontaining 7% glycerol and treated with 20mM taurine, hypotaurine and trehalose. Frozen-thawed sperms were evaluated for sperm motility, viability, membrane integrity, acrosome integrity and sperm penetration ability. The results were compared to semen cryopreserved in tris egg yolk extender containing 7% glycerol only as control. Frozen-thawed semen evaluation clearlyindicated that the addition of taurine or hypotaurine significantly improved (p<0.05) the motility and viability compared to control spermatozoa. Moreover, in membrane integrity, swollen sperm ratio was significantly increased (p<0.05) in taurine, hypotaurine or trehalose compared to control. In sperm acrosome integrity, F pattern ratio was increased (p<0.05) in hypotaurine among treatments, and AR pattern was significantly lowered (p<0.05) in taurine, hypotaurine and trehalose. In assessed sperm fertilizing ability, taurine, hypotaurine or trehalose significantly improved (p<0.05) the ratio of pronucleus formation and SFI. Finally, compared with the control, addition of taurine, hypotaurine or trehalose as an antioxidant to the freezing extender showed more positive effects on the frozen-thawed spermatozoa. It is concluded that the addition of taurine, hypotaurine, or trehalose to the freezing extender could reduce cryodamage of the Korean Jeju Black Bull spermatozoa.

• Journal of Korean Society of Rural Planning
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• v.26 no.2
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• pp.107-116
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• 2020

Up to date, the majority research on the major landscape elements in organic farming has been mainly focused on the practice of seeking efficiency. The problem is that this type of study contributes to polluting the agricultural environment and damaging the ecological circulation system. As an alternative, there is a growing body of research on organic farming, but it is not widely applied that research on how to manage the landscape considering the scenic characteristics of farming villages practicing organic farming. Therefore, this study was carried out in the conservative aspects of rural landscapes in order to effectively manage the landscape of organic agriculture and, intended to be used to maintain and preserve natural and ecologically harmonious landscapes by deriving management methods suitable for landscape elements targeting the major landscape elements of the organic farming complex. To carry out, this study performed the experts survey which is composed of 13 major landscape elements, including rice paddies and fields, monoculture and diverse crops, dirt roads, windbreak trees, accent planting, dum-bung(small pond), natural small river, natural waterways, plastic film houses, one-storied houses, and pavilion. As a result, Farm land was formed in a square shape, concentrated in an independent space, planted companion plants around the crop, and covered with plants to manage the borders. As for the surrounding environment, it was analyzed that the aspart road system circulating through the village, the evergreen broad-leaved windbreak forest around the cultivated land, and the accent plant located at the entrance of the village were suitable. The hydrological environment consists of Round small pond made of stone in an open space, natural rivers around the village, and natural channels around the farmland, and The Major facilities are suitable for greenhouses that are shielded by plants in independent regions, and wooden duck houses located inside the cultivation area are suitable and The settlement facilities were analyzed to be suitable for single-story brick houses located in independent residential areas, pavilion located with greenery in the center of the village, and educational spaces shielded with wood from arable land. If supplementary evaluation criteria suitable for the management of organic farming landscape are additionally supplemented based on the results derived from this study, It is expected to enhance the landscape value of ecologically superior organic farming.

• Journal of Wetlands Research
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• v.15 no.1
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• pp.71-78
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• 2013

An exceptional case of inhabitation of a Sphagnum sp. was firstly confirmed at abandoned paddy terrace (APT) wetland in Ansan. Water sampling for analyzing of physicochemical conditions including nutrients such as NP, $Ca^{2+}$ and $Mg^{2+}$ was performed and the vegetation map for distribution of Sphagnum sp., topographical map, and flora list for companion species were made at field in June 2011. From the results, the Sphagnum sp. in the study site was identified as S. palustre and it covered about 8% of the wetland cover of 3,200 $m^2$. Most distributions of S. palustre were observed at tussock structures as micro-topography by sedges and grasses within a wetland (74%) and the shaded slope area under Pinus densiflora's canopy in wetland boundary (26%). Despite that APT in Ansan is relatively lower wetland in altitude than high moors, the contents of calcium ($0.45{\pm}0.2$) and magnesium ($1.48{\pm}0.6$) ion which are critical limiting factors for Sphagnum spp. were very low levels as well as NP ($PO_4$-P, $0.02{\pm}0.0$; $NO_3$-N, $0.25{\pm}0.3$; $NH_4$-N, $0.06{\pm}0.1$) and it could enable the inhabitation of S. palustre in lower APT.

• Journal of Food Hygiene and Safety
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• v.21 no.4
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• pp.197-203
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• 2006

Parabens are most wildly used in food, cosmetics and pharmaceutic products as preservatives caused of safety and cheap. we had examined that paraben had estrogenic activity through the in vivo and in vitro experiments in last year. We demonstrated that most of parabens(ethyl, butyl, propyl, isobutyl, isopropyl) increased significantly uterus weight as well as induced proliferation of MCF-7 cell and binding of estrogen receptor as endocrine disrupter compounds. In this study, we evaluated that whether parabens have effect on male reproductive system or not. the male rats were administrated parabens by oral injection then examined separation of preputial day for $PND23\simPND52$. As the results, most parabens delayed pubertal development compare to control group. The separation of preputial day of Butyl and Propyl parabens at high concentration were PND 44 days and PND 45days compared to control group as PND 40 days. Even though, parabens as endocrine disrupter wildly spread in food, cosmetics and pharmaceutic products, we didn't have the safe guideline. In abroad, they are re-evaluating safety assessment for parabens. In conclusion, parabens delayed pubertal development in juvenile parabens are consider as endocrine disrupter chemicals.

• Journal of Food Hygiene and Safety
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• v.21 no.4
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• pp.269-273
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• 2006

Potassium Sorbate (PS) is a potassium salt version of sorbic acid, which has antimicrobial and fungistatic features in foods. Therefore, PS is used as a food preservative against fungi and mold. PS has been found to be non-toxic even when taken in large quantities given its trait to be broken down in the body into water and carbon dioxide. Gap Junctional Intercellular Communication (GJIC) is essential in the maintenance of tissue homeostasis during development and differentiation. This study was made of the effects of PS on GJIC in WB-F344 rat liver epithelial (WB) cells. We found dramatic decrease of cell viability in time- and dose-dependent manners when WB cells were treated with PS. The effect of PS on GJIC is strong inhibition, and it took place in parallel with a hyperphosphorylation of connexin 43 expression. The finding that PS interferes with gap junction functionality should be considered with respect to the mechanism of PS-induced hepatotoxicity.

• Animal cells and systems
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• v.15 no.2
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• pp.161-168
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• 2011

Siberian chipmunks, Tamias sibiricus, are one of several popular companion animals found in the pet shops of South Korea. At present, however, there have been no studies done in South Korea examining their origin even though they could be potential carriers of zoonotic diseases, and are a species of concern for efficient conservation and management strategies. Sequences of the mitochondrial cytochrome b gene (1140 bp) were determined to investigate the origin of Siberian chipmunks sold in four South Korean pet shops through comparison with sequence data from animals of known locality. Nine Siberian chipmunks were collected from pet shops in South Korea, which resulted in nine haplotypes. One (AR) of these coincided with the haplotype previously described. Phylogenetic and network analyses using 53 haplotypes including 45 haplotypes from GenBank showed three phylogenetic groups in South Korea, almost concordant to locality, designated as northern, central, and southern parts as described in a previous study. Of the nine individuals examined from the pet shops, eight were clustered into the northern phylogroup but one (cgrb9153) was grouped with the southern phylogroup, implying that at least the Siberian chipmunks examined in this study did not originate from other countries. It is likely that most individuals sold in the pet shops of Seoul were caught in the wild in Gyeonggi-do and Gangwon-do, or are maternal descendants of captive-bred individuals originating from the northern part of South Korea. It is recommended that conservation and management units of Korean chipmunks should be examined in further detail.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.465-470
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• 2007

To evaluate the effect of companion plant (Phlox subulata and Glechoma hederacea var. longituba) on conservation of soil in slopped highland, coverage of crops and soil erosion were investigated with 3 different conditions. The coverage rate of Phlox subulata with Chinese cabbage cultivation was kept on approximately 100% from Chinese cabbage-transplanting to -harvest. The coverage rate of Glechoma hederacea var. longituba with Chinese cabbage cultivation was low at it's early stage, however, reached to approximately 100% at the time of Chinese cabbage harvest. In contrast, the coverage rate of Chinese cabbage cultivation without cover crop, and simultaneous transplanting with Chinese cabbage and cover crop were approximately 60%. Losses of soil and nitrate nitrogen were much lower in Phlox subulata with Chinese cabbage cultivation ($0.1{\sim}0.2ton\;ha^{-1}$, $0.2{\sim}0.4kg\;NO_3{^-}-N\;ha^{-1}$) than those in Chinese cabbage cultivation without cover crop ($20.8ton\;ha^{-1}$, $2.1kg\;NO_3{^-}-N\;ha^{-1}$), and simultaneous transplanting with Chinese cabbage and cover crop ($8.9{\sim}10.5ton\;ha^{-1}$, $1.5{\sim}2.2kg\;NO_3{^-}-N\;ha^{-1}$). Cover plants suppressed the weed occurrence up to about 70%. Live mulching with cover plants set a good effects on weed suppression and reduction of soil and nutrient loss. Therefore intercropping with Phlox subulata will make great contributions to soil conservation in Chinese cabbage cultivated highland.