Browse > Article

Inhibition of Gap Junctional Intercellular Communication by Food Preservatives Potassium Sorbate  

Hwang, Jae-Woong (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Chung, Ji-Hye (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jung, Ji-Won (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Jung, Ji-Youn (Department of Companion and Laboratory Animal Science, Kongju National University)
Kim, Sun-Jung (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Park, Jung-Ran (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Ahn, Ji-Yun (Food Function Research Division, Korea Food Research Institute)
Ha, Tae-Youl (Food Function Research Division, Korea Food Research Institute)
Kim, Sung-Ran (Food Function Research Division, Korea Food Research Institute)
Lee, Yong-Soon (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Kang, Kyung-Sun (Laboratory of Stem Cell and Tumor biology, Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Food Hygiene and Safety / v.21, no.4, 2006 , pp. 269-273 More about this Journal
Abstract
Potassium Sorbate (PS) is a potassium salt version of sorbic acid, which has antimicrobial and fungistatic features in foods. Therefore, PS is used as a food preservative against fungi and mold. PS has been found to be non-toxic even when taken in large quantities given its trait to be broken down in the body into water and carbon dioxide. Gap Junctional Intercellular Communication (GJIC) is essential in the maintenance of tissue homeostasis during development and differentiation. This study was made of the effects of PS on GJIC in WB-F344 rat liver epithelial (WB) cells. We found dramatic decrease of cell viability in time- and dose-dependent manners when WB cells were treated with PS. The effect of PS on GJIC is strong inhibition, and it took place in parallel with a hyperphosphorylation of connexin 43 expression. The finding that PS interferes with gap junction functionality should be considered with respect to the mechanism of PS-induced hepatotoxicity.
Keywords
Potassium Sorbate; Gap Junctional Intercellular Communication; hepatotoxicity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Perez-Prior, M.T., Manso, J.A., Garcia-Santos, Mdel P., Calle E. and Casado J.:Alkylating potential of potassiun sorbate. J. Agric. Food. Chem., 53, 10244-10247 (2005)   DOI   ScienceOn
2 Kumar, N.M. and Gilula, N.B.: The gap junction communication channel. Cell, 84, 381-388 (1996)   DOI   ScienceOn
3 Simon, A.M. and Goodenough, D.A.: Diverse functions of vertebrate gap junctions. Trends Cel.l Biol., 8, 477-483 (1998)   DOI   ScienceOn
4 Hwang, J.W., Park, J.S., Jo, E.H., Kim, S.J., Yoon, B.S., Kim, S.H., Lee, Y.S. and Kang, K.S.. : Chinese cabbage extracts and sulforaphane can protect $H_2O_2$-induced inhibition of gap junctional intercellular communication through the inactivation of ERK1/2 and p38 MAP kinases. J. Agric. Food. Chem., 53, 8205-8210 (2005)   DOI   ScienceOn
5 Thakur, B.R., Singh, K. and Arya, S.S.: Chemistry of Sorbates - A Basic Perspective. Food Rev. Int., 10, 71-91 (1994)   DOI   ScienceOn
6 el-Fouly, M.H., Trosko, J.E., Chang, C.C.: Scrape-loading and dye transfer. A rapid and simple technique to study gap junctional intercellular communication. Exp. Cell Res., 168, 422-430 (1987)   DOI   ScienceOn
7 Musil, L. S.; Cunningham, B.A., Edelman, G,M. and Goodenough, D.A.: Differential phosphorylation of the gap junction protein connexin43 in junctional communication-competent and -deficient cell lines. J. Cell Biol., 111, 2077-2088 (1990)   DOI