• Reproductive and Developmental Biology
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• v.33 no.1
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• pp.63-69
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• 2009

In this study we tried to construct a more efficient tetracycline-inducible gene expression system by replacing previous key elements with more advance ones. At the beginning, we substituted PGK (phophoglycerate kinase) promoter for CMV (cytomegalovirus) promoter to control "$rtTA2^sM2$" which has been known for high induction efficiency in response to tetracycline. With this modification, expression of the EGFP marker gene under the induction condition was significantly increased. Next, we replaced "TRE" fragment with a modified version named "TRE-tighf" which has been reported to have higher affinity and specificity to the transactivator by minor base change of the "TRE" DNA fragment sequence. Use of "TRE-tighf" instead of "TRE" resulted in more than 10 fold increment in terms of induction efficiency and significant decrement of background expression in non-inducible condition. By combining PGK promoter and "TRE-tight" fragment, we could upgrade previous tetracycline-inducible system to show more stringent turn on/off gene switch ability and stronger expression of the gene of our interest. Use of this newly developed system must be very helpful to the studies of gene expression, especially to the transgenic animal study in which non-controllable constitutive expression of the transgene has been one of the urgent problems to be solved.

• Journal of Biomedical Engineering Research
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• v.28 no.5
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• pp.609-617
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• 2007

In this paper, a new rehabilitation training system was developed to improve equilibrium sense of the elderly by combining virtual reality technology with a fixed exercise bicycle. In order to evaluate the effectiveness of the training system, the elderly participated as test subject in the investigation of the influence of different the parameters on postural balance control. We measured three different running modes of virtual bicycle system with two successive sets. The parameters measured were running time, velocity, the weight movement, the degree of the deviation from the road, and the variables about the center of pressure. The repeated training, our results showed that the running capability of the elderly improve compared. In addition, it was found out that the ability of postural control and the equilibrium sense was improved with the presentation of the visual feedback information of the distribution of weight. From the results of this experiment, we showed that our newly developed system might be useful in the diagnosis of equilibrium sense or in the improvement of the sense of sight and, somatic, and vestibular sense of the elderly in the field of rehabilitation training.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.1055-1060
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• 2009

In this paper, we design an e-Learning system for the Driver's License Teste studying through the Internet. The proposed system make users to be arrived at the goal for the license in a shorter time by offering learning contents and items according to the item-responses made by the users based on the Item Response Theory. Moreover we design the scheme to give the optimum items and the most necessary content to the user during the learning procedure in the form of concept-based objects. All the items in the problem bank DB maintain their difficulties, discriminations, and guessing parameters as is the case of 3-parameter logistic model. In addition user profile DB stores users' status informations, item responses, and ability parameters. Using these structures and combining agents, we can offer the optimum learning process or dynamic personalized studying structure to the user. We can construct interface agent and content selection and feedback agent with the DB's described above. User can study without any awareness of system operations or personal fitting scheme.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.116-116
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• 2018

Bioconversion, the enzymatic process by microorganism on organic precursor to desired products. The natural extract is converted into a form that can be easily absorbed into the skin, while scaling up of to higher quantity is possible. Selection of naturally processed raw material rather than chemically processed is preferred. Therefore, fermentation was carried out by mixing Rubus coreanus Miquel, soybean, Zanthoxylum schinifolium as bioconverting materials, the possibility of inflammation, whitening material were checked. In this study, useful microorganisms were isolated from various salted fish, and 16S rDNA sequence was analyzed to confirm their genetic characteristics. Combining the three natural materials using bioconversion technology to study their activity before and after fermentation. To evaluate the antioxidant activity and the active ingredient content the DPPH radical scavenging activity and the total polyphenol content were examined. Raw 264.7 cells were used to evaluate MTT assay, NO and $TNF-{\alpha}$ production inhibitory activity. Also, to evaluate the whitening activity, tyrosinase inhibitory activity and melanin formation inhibitory activity were measured using B16F10 cells. In total 34 strains were obtained from various salted fish. The effective strains useful for the bioconversion process, showed that DPPH radical scavenging ability and polyphenol content were increased in the two kinds of microbial treatment groups compared to the untreated group. 16S rDNA sequencing analysis of the strains showed excellent in Pediococcus pentosaceus B1 in comparison. An increase of up-to 156% in anti-oxidative activity and 141% in polyphenol content was observed after bioconversion. In addition, after mixed fermentation the toxidty of Raw 264.7 and B16F10 cells tended to decrease and a significant increase was observed in anti-inflammatory activity as well. Also, tyrosinase activity and melanin significantly. synthesis decreased significantly.

• Journal of Digital Convergence
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• v.11 no.4
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• pp.229-234
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• 2013

The purpose of this study is to develop a lighting apparatus of the illuminance and color temperature to maximize the ability of the optimal combination of light sources that can be controlled efficiently control device. Finding people comfortable feeling for indoor lighting that can be used in a variety of color temperature illumination area by combining light sensitivity can be realized. Lighting apparatus for fluorescent lamps with different color temperature of 2000K and 8000K, and by varying the quantity of each of the fluorescent lamps, the illuminance of lighting equipment and color temperature through optical simulations were evaluated. By infrared remote control receiver, divided into 5 types of relaxation, conversation, meeting, hospitality, arts and the lighting environment you want to transfer the PC0 ~ PC4 through the parallel port on the mode selected by the user at the receiving end the DC voltage output. EEFL inverter input DC voltage and the DC input voltage, depending on the level of EEFL dimming value (illuminance and color temperature) lighting environment you want to create change while using a PIR sensor EEFL automatically turn off if people do not have was developed so that the power consumption so you can save.

• Journal of the Korean Data and Information Science Society
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• v.18 no.2
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• pp.327-344
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• 2007

Ridge partial least squares regression (RPLS) is a regression method which can be obtained by combining ridge regression and partial least squares regression and is intended to provide better predictive ability and less sensitive to overfitting. In this paper, explicit expressions for the shrinkage factor of RPLS are developed. The structure of the shrinkage factor is explored and compared with those of other biased regression methods, such as ridge regression, principal component regression, ridge principal component regression, and partial least squares regression using a near infrared data set.

• 한국정보디스플레이학회:학술대회논문집
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• 2006.08a
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• pp.647-650
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• 2006

Low temperature polycrystalline silicon (LTPS) technology using a high power laser have been widely applied to thin film transistors (TFTs) for liquid crystal, organic light emitting diode (OLED) display, driver circuit for system on glass (SOG) and static random access memory (SRAM). Recently, the semiconductor industry is continuing its quest to create even more powerful CPU and memory chips. This requires increasing of individual device speed through the continual reduction of the minimum size of device features and increasing of device density on the chip. Moreover, the flat panel display industry also need to be brighter, with richer more vivid color, wider viewing angle, have faster video capability and be more durable at lower cost. Kornic Systems Co., Ltd. developed the $KORONA^{TM}$ LTP/GLTP series - an innovative production tool for fabricating flat panel displays and semiconductor devices - to meet these growing market demands and advance the volume production capabilities of flat panel displays and semiconductor industry. The $KORONA^{TM}\;LTP/GLTP$ series using DPSS laser and XeCl excimer laser is designed for the new generation of the wafer & FPD glass annealing processing equipment combining advanced low temperature poly-silicon (LTPS) crystallization technology and object-oriented software architecture with a semistandard graphical user interface (GUI). These leading edge systems show the superior annealing ability to the conventional other method. The $KORONA^{TM}\;LTP/GLTP$ series provides technical and economical benefits of advanced annealing solution to semiconductor and FPD production performance with an exceptional level of productivity. High throughput, low cost of ownership and optimized system efficiency brings the highest yield and lowest cost per wafer/glass on the annealing market.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.24 no.1
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• pp.52-56
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• 2011

The ongoing Superconducting Fault Current Limiter(SFCL) development mainly has focused on the application of commercializaton and power system through combining with normal-conducting device, moving away from current-limiting method, which is solely dependant on the existing superconductor. Compared to the structural development above, on the other hand, the research on applying superconducting current-limiting element to SFCL, the heart of SFCL, still has a lot left to do, apart form traditional resistive type SFCL. In this study, we looked into the current limiting characteristic of SFCL using core and coil. YBCO coated conductor with stainless steel stabilizer layer was verified by the excellent of current-limiting element of the resistive type SFCL that has a high Jc and index as well as being superior in mechanical property. Also, we study temperature characteristics and resistance characteristics, max voltage, response time and current-limiting ability that can be an indicator as current-limiting element while applying to superconducting current-limiting element caused by variation of winding direction, winding ratio of SFCL using core and coil.

• Journal of the Korea Convergence Society
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• v.9 no.7
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• pp.205-211
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• 2018

The purpose of this study is to propose a strategy to educate the core contents of various major fields in practical and convergent ways in universities in order to prepare for the 4th industrial revolution era. First, convergence field is established in the university curriculum in addition to the existing major and the liberal arts field. Second, convergence courses are created in connection with job ability, which are major curriculum but only for students in other departments. Third, convergence courses are organized in the form of module curriculum, which is a small-sized and independent curriculum consisting of 4 courses. D University, one of the local private universities in Korea, has provided such convergence module education since 2016, and satisfactory results have been obtained in the course enrollment rate and the course evaluation, etc. By combining these convergence modules in various ways, it is possible to easily create customized majors required and designed by local companies at low cost.

• The Journal of Korean Medicine
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• v.29 no.3
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• pp.11-20
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• 2008

Objectives: Warm-needling is a method combining the effects of acupuncture with the effects of moxibustion. We need to standardize the characteristics of warm-needling in order to achieve consistency in its operational mechanisms and effects, which will improve clinical ability in the field of Eastern medicine. Methods: In this study, using the LabVIEW system on the warm-needling technique, we measured and compared partial temperature changes which varied according to the density of the moxa cone and the moxa cone's core peak temperature. Results& Conclusions: Examination of the warm-needle's partial temperature in relation to the cone density of the 0.8g moxa specimen suggests that a lower density of the moxa cone corresponds to a higher peak temperature and but with a shorter duration. During the effective stimulus time, the lower the density of the moxa cone, the shorter the duration of the effective stimulus time and the higher the mean temperature. Conversely, the higher the density of the moxa cone, the longer the effective stimulus time and lower the mean temperature.