• Title/Summary/Keyword: combining ability

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Enzymatic Hydrolysis for Effective Extraction of Antioxidative Compounds from Hizikia fusiformis

  • Siriwardhana, Nalin;Jeon, You-Jin;Kim, Soo-Hyun;Ha, Jin-Hwan;Heo, Soo-Jin;Lee, Ki-Wan
    • ALGAE
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    • v.19 no.1
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    • pp.59-68
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    • 2004
  • Hizikia fusiformis hydroysates by five carbohydrases (Viscozyme, Celluclast, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase) were investigated for their extraction efficacy (yield and total total polyphenolic content) and antioxidative activity (DPPH radical and hydrogen peroxide scavenging activity). Termamyl and Ultraflo of the carbohydrases and Flavourzyme and Alcalase of proteases were selected by their high eficacy of extraction and antioxidative activity. Selected enzymes were used to investigate the optimum enzymatic reaction time and dosage (enzyme/substrate ratio) suitable for hydorolysis. Optimum reaction time for the enzymatic hydrolysis was 3 days and optimum dosage of hydrolysis was observed as 5%. Simultaneously, Ultraflo of the two carbohydrases and Alcalse of the two proteases were selected as the most effective enzymes. Combination of Ultraflo and Alcalase under optimum hydrolysis conditions could intensify the extraction efficacy of antioxidative materials form H. fusiformis. The hydrolysate obtained by combining the enzymes was separated into four different molecular weight fractions (<1kD, 1-10 kD, 10-30 kD and >30 kD) and recorded the polyphenolic content distribution and respective antioxidative ability. The fraction <1kD was identified as less effective and those fractions > 1kD indicated comparatively higher antioxidative activities related to their polyphenolic content.

Breeding of a New Silkworm Variety, Kumhwangjam, with a Sex-Limited Cocoon Color for Spring Rearing Season

  • Kang, Pil Don;Sohn, Bong Hee;Lee, Sang Uk;Kim, Mi Ja;Jung, I Yeon;Kim, Young Soon;Kim, Yung Dae;Lee, Heui Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.89-93
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    • 2004
  • A new silkworm variety, Kumhwangjam, with a sex-limited cocoon color for spring rearing season is F$_1$ hybrid between Jam 309, a Japanese race bred from Y1/9153, and Jam 310, a Chinese race from Y1/8586. In the local adaptability test performed at 8 local areas in spring rearing season of 2003, major characteristics of Kumhwangjam were mainly similar to those of the check variety Kumokjam. The new silkworm variety, Kumhwangjam, showed a sex-limited cocoon color: yellow for female and white for male with elliptical cocoon shape. This sex-limited cocoon color can be used as a valuable marker for discrimination between female and male for various purposes.

Practice of industrial strain improvement (제 1차 한.중 생명공학 심포지움)

  • Lei, Zhao-zu
    • The Microorganisms and Industry
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    • v.19 no.2
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    • pp.34-41
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    • 1993
  • Industrial strain improvement is concerned with developing or modifying microorganisms used in production of commercially important fermentation products. The aim is to reduce the production cost by improving productivity of a strain and manipulating specific characteristics such as the ability to utilize cheaper raw materials or resist bacteriophages. The traditional empirical approach to strain improvement is mutation combined with selection and breeding techniques. It is still used by us to improve the productivity of organisms in amino acids, organic acids and enzymes production. The breeding of high L-lysine-producing strain Au112 is one of the outstanding examples of this approach. It is a homoserine auxotroph with AEC, TA double metabolic analogue resistant markers. The yield reaches 100 g/l. Besides, the citric acid-producing organism Aspergillus niger, Co827, its productivity reaches the advanced level in the world, is also the result of a series mutations especially with $^60Co{\gamma}$-radiation. The thermostable .alpha.-amylase producing strain A 4041 is the third example. By combining physical and chemical mutations, the strain A 4041 becomes an asporogenous, catabolite derepressed mutant with rifamycin resistant and methionine, arginine auxotroph markers. The .alpha.-amylase activity reaches 200 units/ml. The fourth successful example of mutation in strain improvement is the glucoamylase-producing strain Aspergillus niger SP56, its enzyme activity is 20,000 units/ml, 4 times of that of the parental strain UV-11. Recently, recombinant DNA approach provides a worthwhile alternative strategy to industrial strain improvement. This technique had been used by us to increase the thermostable .alpha.-amylase production and on some genetic researches.

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A Study on the Prediction Diagnosis System Improvement by Error Terms and Learning Methodologies Application (오차항과 러닝 기법을 활용한 예측진단 시스템 개선 방안 연구)

  • Kim, Myung Joon;Park, Youngho;Kim, Tai Kyoo;Jung, Jae-Seok
    • Journal of Korean Society for Quality Management
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    • v.47 no.4
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    • pp.783-793
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    • 2019
  • Purpose: The purpose of this study is to apply the machine and deep learning methodology on error terms which are continuously auto-generated on the sensors with specific time period and prove the improvement effects of power generator prediction diagnosis system by comparing detection ability. Methods: The SVM(Support Vector Machine) and MLP(Multi Layer Perception) learning procedures were applied for predicting the target values and sequentially producing the error terms for confirming the detection improvement effects of suggested application. For checking the effectiveness of suggested procedures, several detection methodologies such as Cusum and EWMA were used for the comparison. Results: The statistical analysis result shows that without noticing the sequential trivial changes on current diagnosis system, suggested approach based on the error term diagnosis is sensing the changes in the very early stages. Conclusion: Using pattern of error terms as a diagnosis tool for the safety control process with SVM and MLP learning procedure, unusual symptoms could be detected earlier than current prediction system. By combining the suggested error term management methodology with current process seems to be meaningful for sustainable safety condition by early detecting the symptoms.

Genetic Improvement of Some Traits in Four Strains of Silkworm, Bombyx mori L.

  • Moghaddam S. H. Hosseini;Jomeh K. N. Emam;Mirhosseini S. Z.;Gholamy M. R.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.10 no.2
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    • pp.95-99
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    • 2005
  • A breeding plan was carried out on four commercial strains of silkworm (Bombyx mori L.) 101, 102, 103 and 104 to improve some important traits. Genetic gain or response to selection $({\Delta}G)$, heritability of cocoon shell weight (CSW) and specific combining ability effects were estimated to determine the strains that can be improved. Strain 101 had lowest heritabitity, ${\Delta}G$ and viability. Strain 102 was acceptable in selection response but its viability was low. Therefore these two strains were not suitable for more selection. As a result, only lines 103 and 104 were chosen for further improvement. Intra population selection based on independent culling level method practiced from third to sixth generation for both productive and viability traits simultaneously. While CSW and CW had increasingly enhanced during primary generations, they went slightly up after third generation. According to negative genetic correlation, viability decreased during primary generations, but after third generation that paid attention to balanced development of both productive and viability traits, viability increased so that the pupation rate reached to $91\%$ in 103 and $97\%$ in 104 for last generation $(G_8)$.

Bagging deep convolutional autoencoders trained with a mixture of real data and GAN-generated data

  • Hu, Cong;Wu, Xiao-Jun;Shu, Zhen-Qiu
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.13 no.11
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    • pp.5427-5445
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    • 2019
  • While deep neural networks have achieved remarkable performance in representation learning, a huge amount of labeled training data are usually required by supervised deep models such as convolutional neural networks. In this paper, we propose a new representation learning method, namely generative adversarial networks (GAN) based bagging deep convolutional autoencoders (GAN-BDCAE), which can map data to diverse hierarchical representations in an unsupervised fashion. To boost the size of training data, to train deep model and to aggregate diverse learning machines are the three principal avenues towards increasing the capabilities of representation learning of neural networks. We focus on combining those three techniques. To this aim, we adopt GAN for realistic unlabeled sample generation and bagging deep convolutional autoencoders (BDCAE) for robust feature learning. The proposed method improves the discriminative ability of learned feature embedding for solving subsequent pattern recognition problems. We evaluate our approach on three standard benchmarks and demonstrate the superiority of the proposed method compared to traditional unsupervised learning methods.

Representing Fuzzy, Uncertain Evidences and Confidence Propagation for Rule-Based System

  • Zhang, Tailing
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.1254-1263
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    • 1993
  • Representing knowledge uncertainty , aggregating evidence confidences , and propagation uncertainties are three key elements that effect the ability of a rule-based expert system to represent domains with uncertainty . Fuzzy set theory provide a good mathematical tool for representing the vagueness associated with a variable when , as the condition of a rule , it only partially corresponds to the input data. However, the aggregation of ANDed and Ored confidences is not as simple as the intersection and union operators defined for fuzzy set membership. There is, in fact, a certain degree of compensation that occurs when an expert aggregates confidences associated with compound evidence . Further, expert often consider individual evidences to be varying importance , or weight , in their support for a conclusion. This paper presents a flexible approach for evaluating evidence and conclusion confidences. Evidences may be represented as fuzzy or nonfuzzy variables with as associat d degree of certainty . different weight can also be associated degree of certainty. Different weights can also be assigned to the individual condition in determining the confidence of compound evidence . Conclusion confidence is calculated using a modified approach combining the evidence confidence and a rule strength. The techniques developed offer a flexible framework for representing knowledge and propagating uncertainties. This framework has the potention to reflect human aggregation of uncertain information more accurately than simple minimum and maximum operator do.

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Construction of Resource Recovery System for Organic Wastes (유기성 폐기물의 자원화 체제구축에 관한 연구)

  • 양재경;최경민
    • Journal of Korea Technology Innovation Society
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    • v.2 no.2
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    • pp.290-308
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    • 1999
  • In this study a system for the treatment or recyling of organic wastes from both urban and rural area was recommended. It was developed based on the resource recovery system regarding human being by four tectnologies; forage, methane production, high-grade composting and complete decomposition. High quality compost can be produced by combining several kind of wastes produced from urban and agricultural areas. High quality compost must possess not only general characteristics of ordinary compost, but also a superior ability to improve the soil properties and must contain more nutrients for plant. Cedar chips were recommended as the main bulking agent to adjust moisture contents and air permeability. Charcoal and zeolite can be used not only as the second bulking agent but also as fertilizer for improve the soil amendment. Complete decomposition of organic wastes is defined by organic matter being completely converted to $CO_2$ and water. All the input water was evaporated by the heat produced through the oxidation of organic matter, In the present study, the complete treatments were successfully achieved for Shochu wastewater, swine wastes, thickened excess sewage sludge, wastes produced by Chinese restaurant and anaerobic digested sludge. First of all, recycling center of organic wastes should be established for the protect the environments and effective recovery of organic resources. This may means the way to derive the recovery of human value.

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Development of the Activity Posture Classifier for Ubiquitous Health Care (유비쿼터스 헬스케어를 위한 활동상태 분류기 개발)

  • Kim, Se-Jin;Chung, Wan-Young;Jeong, Do-Un
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2007.06a
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    • pp.703-706
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    • 2007
  • The real-time monitoring about the activity of the human provides useful information about the activity quantity and an ability. This study developed a system for human physical activity assessment in ambulatory monitoring using portable sensing device combining a tri-axial accelerometer and wireless sensor node. This real-time system is able to identify several postures, posture transitions and movements with classification algorithm. In addition, this system also features fall detection capability. The results of the assessment for evaluating the performance of the system show high identification accuracy.

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PWM Variable Carrier Generating Method for OEW PMSM with Dual Inverter and Current Ripple Analysis according to Zero Vector Position (듀얼 인버터 개방 권선형 영구자석 동기 전동기 제어를 위한 PWM 가변 캐리어 생성법 및 영벡터 위치에 따른 전류 리플 분석)

  • Shim, Jae-Hoon;Choi, Hyeon-Gyu;Ha, Jung-Ik
    • The Transactions of the Korean Institute of Power Electronics
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    • v.25 no.4
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    • pp.279-285
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    • 2020
  • An open-end winding (OEW) permanent magnet synchronous motor with dual inverters can synthesize large voltages for a motor with the same DC link voltage. This ability has the advantage of reducing the use of DC/DC boost converters or high voltage batteries. However, zero-sequence voltage (ZSV), which is caused by the difference in the combined voltage between the primary and secondary inverters, can generate a zero-sequence current (ZSC) that increases system losses. Among the methods for eliminating this phenomenon, combining voltage vector eliminated ZSV cannot be accomplished by the conventional Pulse Width Modulation(PWM) method. In this study, a PWM carrier generation method using functionalization to generate a switching pattern to suppress ZSC is proposed and applied to analyze the control influence of the center-zero vector in the switching sequence about the current ripple.