• Animal Bioscience
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• v.35 no.6
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• pp.814-825
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• 2022

Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland. Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional annotation and weighted gene co-expression network analysis (WGCNA). Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day -2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day -14, day -2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved. Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine.

• Korean Journal of Agricultural Science
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• v.22 no.1
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• pp.82-95
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• 1995

These experiments were carried out to isolate SIgA from human and bovine milk. The immunochemical properties of SIgA from human and bovine milk were examined by Gel filtration, DEAE and SDS-PAGE. Double Immunodiffusion, and Immunoelectrophoresis. The results obtained are as follows: 1. Human SIgA was purified from colostrum of Korean women by repeated gel filtration on Sephadex G-200 and Sepharose 6B, but bovine SigA was not cleary purified from bovine colostrum of Holstein cows by anion exchange chromatography using DEAE-Sephadex A-50 and gel filtration on Sephadex G-200 and Sepharose 6B. 2. The immunochemical properties of fractions from gel filtration on the Sephadex G-200 and Sepharose 6B column as assessed by Immunoelectrophoresis and double Immunodiffusion to identify the presence of IgM in first peak fraction, and the presence of pure SIgA in second peak fraction. However, Bovine SigA rich fraction from bovine colostrum of Holstein cows contained a large amount of $IgG_1$-dimer in addition to SIgA. 3. The fragments of reduced bovine colostrum SIgA rich fraction were estimated to have molecular weights of secretory component, heavy chain and light chain (75,000-80,000, 50,000-60,000, 25,000-27,000 daltons) by SDS-PAGE, respectively. Those were similar to the molecular weight of reduced SIgA from human milk.

• Journal of Animal Science and Technology
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• v.65 no.6
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• pp.1308-1322
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• 2023

Minerals is required small amounts among various nutrients, but it has a significant impact on sow longevity and reproduction performance. This study was carried out to see the beneficial effects of marine-derived Ca-Mg complex on the reproductive performance of sows during four-parity periods. Seventy-two gilts ([Yorkshire × Landrace] × Duroc), with an average body weight of 181 kg, were randomly allocated to three groups; CON (basal diet), 0.3LC (CON - MgO - 0.3% limestone + 0.4% Ca-Mg complex), and 0.7LC (CON - MgO - 0.7% limestone + 0.4% Ca-Mg complex). During parity 3 and 4, the expression level of SCD gene was lower in the umbilical cord of piglets born to 0.3LC and 0.7LC sows compared with the CON sows. During parity 2, 3 and 4, SLC2A2 and FABP4 gene expressions were higher in the umbilical cord of piglets born to 0.7LC sows and the placenta of sows from 0.3LC groups, respectively. Ca-Mg complex increased (p < 0.05) Ca and Mg concentrations in sows and their piglets' serum as well as in colostrum regardless of parities. The serum vitamin D concentration was higher (p < 0.05) in their first parity, whereas serum prolactin and estrogen concentrations were higher (p < 0.05) during the fourth and third parity, respectively. The growth hormone concentrations were higher (p < 0.05) in the piglets born to sows during the first and second parity. The fat and immunoglobulin A (IgA) concentrations in colostrum were higher (p < 0.05) during the third and fourth parity, respectively. A reduction (p < 0.05) in salivary cortisol, epinephrine, and norepinephrine concentrations was observed in 0.3LC and 0.7LC sow groups compared with CON after farrowing regardless of parity, however before farrowing, a reduction in norepinephrine was observed. Before farrowing, the epinephrine and norepinephrine concentrations were higher (p < 0.05) during the first and second parity. After farrowing, the concentration of these hormones was higher during the second parity. Taken together, sows' parity and dietary Ca-Mg complex supplementation influenced serum metabolites, colostrum nutrients, stress hormones as well as the gene expressions related to lipid and glucose metabolism.

• Journal of the Korean Home Economics Association
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• v.24 no.4
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• pp.51-55
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• 1986

The composition of human milk was compared with that of cow's milk. The contents of amino acids were analyzed by amino acid autoanalyzer. The content of glutamic acid in cow's milk was three times as much as that in human milk. The content of essential amino acid in human colostrum was twice as much as that in mature milk.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.2
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• pp.133-138
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• 1998

Plasma and milk samples of 24 periparturient Holstein cows were collected from 1 week prepartum to 10 week postpartum to measure the effects of parity on mineral status in periparturient cows. Cows were fed mainly mixed ration with the concentrate supplement during the experimental period to meet nutrient requirement of dairy cattle for TDN, protein, and minerals, Plasma Ca of cows decrease as parity increased, but plasma Mg, K, Fe and Zn were lowest in the first lactation cows. Plasma inorganic P, Na, and alkaline phosphatase were not affected by the parity. Plasma Ca, Fe and Zn of cows decreased at parturition, but plasma Mg increase. Plasma Ca of the first, second and third and more lactation cows at parturition were 9.65, 8.96, and 8.92 mg/dl, respectively. Colostral Ca, P, Mg, Na, and Zn were highest in the first lactation cows, although colostrum yield was lower. Milk yield from 1 to 10 weeks postpartum was lowest in the first lactation cows, but mineral concentrations in milk were not affected by the parity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.1
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• pp.47-50
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• 1985

Changes in fatty acid composition of phospholipids from human milk during the course of lactation were determined in milk from Korean lactating mothers giving birth at term. The levels of 8 : 0 increased significantly from colostral to transitional stages with a further small decrease at mature stage. The proportions of 10 : 0, 12 : 0 and 14 : 0 increased markedly from colostrum to transitional to mature milk. The w6-derived long-chain polyunsaturated fatty acids, 20 : 2w5, 20 : 4w6 and 22 : 4w5, were decreased with time of lactation. The levels of 24 : 1w9 were significantly higher in transitional milk than in colostrum and then significantly decreased to lowers than the colostral levels at mature milk.

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.595-602
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• 2006

The purpose of this study is to demonstrate antimicrobial activities of the lactoferrin and its peptic hydrolysates obtained from the colostrums of Hanwoo(Korean native cattle) and Holstein cattle. In the measurement of antimicrobial activity to E. coli O111 and other microorganisms, bovine lactoferrin showed a higher antimicrobial activity than that of Hanwoo cow's lactoferrin . The minimal inhibitory concentration (MIC) of lactoferrin against E. coli O111 was exhibited 1.5mg/ml(Holstein) and 2.75mg/ml (Hanwoo). The same result was also observed between bovine lactoferrin hydrolysate (0.12mg/ml) and Hanwoo cow's lactoferrin hydrolysate (0.25mg/ml). In addition of lysozyme, antimicrobial activity of lactoferrin was increased.

• Korean Journal of Community Nutrition
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• v.1 no.3
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• pp.433-440
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• 1996

This study was carried out using a questionnaire in order to investigate factors affecting the status and the method of infant feeding in Incheon area. The subjects were 126 monthers of infants ranging from 3 months to 18 months. The results are : the percentages of those feeding colostrum and breast milk has decreased in comparison with the previous results. But the percentage of breast-feeding gets higher in a case where colostrum or breast milk is first fed after delivery. Also frequency and birth order of child plays a part : the more frequently monthers try to feed breast milk in the earliest period of feeding, the more they keep breast-feeding, and the latter-born(the second-born or the third born) children get more chances to be breast-fed than the first-born. Another noticeable factor is what babies feed on during hospitalization. if they get fed more breast milk than infant formula while they are in hospital, they tend to keep feeding on breast milk after they come back home. Breast-feeding group have good knowledge and information abut the importance of breast-feeding. The data shows that they believe the superiority of breast milk.

• Korean Journal of Veterinary Research
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• v.47 no.1
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• pp.1-6
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• 2007

The importance of milk for the growth and health of a newborn offspring is well known. Milkcontains immunoglobulin G (Ig G), Ig A, lactoperoxidase, lactoferin, cytokines, and growth factors.Osteopontin, one of the multifunctional proteins, is secreted by macrophages, T cel, and epithelial cells.bovine milk have not been clarified. The aim of this study was to observe the expression of osteopontin,in bovine milk during the lactation period or bovine mamary glands..Western blot analysis detected thatosteopontin was expressed in bovine milk whey and mamary glands. The expression level of osteopontinin colostrum whey was higher than those in early milk and mature milk whey. Immunohistochemistryshowed that osteopontin was detected in the glandular epithelium and epithelial cels of intralobular ductof mamary glands. These findings suggest that osteopontin transiently shows high expression in colostrumand plays a potential role in the immunological development of breast-fed calves.

• Journal of Dairy Science and Biotechnology
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• v.26 no.2
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• pp.1-8
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• 2008

Human milk contains the nutrients for the growth and the development of the newborn. It contains specific proteins, lipids, and other components designed to be easily digestible and which play important roles in infant nutrition. However, when the lactation is failure or milk secretion is not enough, bovine milk based dried formulations, which is called infant formula, have been widely used for infant feeding. In this study, we investigated protein composition and amino acids profile related to easy digestion; fatty acids composition and LCPUFA(DHA and ARA) related to brain and eye development; synbiotics which take advantage of both probiotics and prebiotics; and colostrum-originated functional micronutrient as major issues in the recent infant formula. Domestic infant formulas, which are based on the research of human and bovine milk rich in functional components for immunity, growth, anti-allergenicity, digestion and brain development, have been developed to be similar to human milk.