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http://dx.doi.org/10.5187/JAST.2006.48.4.595

Antimicrobial Activities of Lactoferrin and its Hydrolysate Obtained from the Colostrum of Hanwoo and Holstein Cattle  

양희진 (Department of Food Science & Biotechnology, Sungkyunkwan University)
이수원 (Department of Food Science & Biotechnology, Sungkyunkwan University)
Publication Information
Journal of Animal Science and Technology / v.48, no.4, 2006 , pp. 595-602 More about this Journal
Abstract
The purpose of this study is to demonstrate antimicrobial activities of the lactoferrin and its peptic hydrolysates obtained from the colostrums of Hanwoo(Korean native cattle) and Holstein cattle. In the measurement of antimicrobial activity to E. coli O111 and other microorganisms, bovine lactoferrin showed a higher antimicrobial activity than that of Hanwoo cow's lactoferrin . The minimal inhibitory concentration (MIC) of lactoferrin against E. coli O111 was exhibited 1.5mg/ml(Holstein) and 2.75mg/ml (Hanwoo). The same result was also observed between bovine lactoferrin hydrolysate (0.12mg/ml) and Hanwoo cow's lactoferrin hydrolysate (0.25mg/ml). In addition of lysozyme, antimicrobial activity of lactoferrin was increased.
Keywords
Lactoferrin; Hanwoo; Colostrum; Antimicrobial activity;
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1 Aisen, P. and Listowsky, I. 1980. Iron transport and storage proteins. Annu. Rev. Biochem. 49
2 Arnold. R. R.. Cole. M. F. and McGhee. J. R. 1977. A bactericidal effect for human Iactoferrin. Science. 197:263-265   DOI
3 Bellamy. W.. Wakabayashi. H., Takase, M.. Kawase. K.. Shimamura, S. and Tomita. M. 1993. Killing of Candida albicans by lactoferricin B. a potent antimicrobial peptide derived from the N-terminal region of bovine lactoferrin Med. Microbiol. Immunol. 182:97-105
4 Bellamy. W.. Yamauchi. K.. Wakabayashi. H., Takase, M.. Takakura, N.. Shimamura, S. and M. Tomita. 1994. Antifungal properties of lactoferricin B. a peptide derived from the N-terminal region of bovine lactoferrcin. Lett. Appl. Microbiol. 18: 230-233   DOI   ScienceOn
5 Brock. J. H. 1985. Part I. I: Metal proteins with non-redox roles: In Metalloproteins. P. Harrison (Ed.). Macmillan Press. London. U.K., p183-262
6 Bullen. J. J. and Armstrong. J. A. 1979. The role of lactoferrin in the bactericidal function of polymorphonuclear leucocytes. Immunology. 36: 781-791
7 Chen. J. P. and Wang. C. H. 1991. Microfiltration affinity purification of lactoferrin and immunoglobulin G from cheese whey. J. Food Sci. 56:701-706   DOI
8 Dionysius. D. A.. Herse. J. B. and Grieve. P. A. 1991. Extraction of lactoperoxidase and lactoferrin from whey using batch ion exchange techniques. Aust, J. Dairy Technol. 46(Nov): 72-76
9 Gurr. M. I. 1981. Review of the progress of dairy science: human and artificial milks for infant feeding. J. Dairy Res. 48:519-554   DOI   ScienceOn
10 Lee. S. S.. Ko. S. B.. Oh, W. Y., Yang, Y. B.. Kim. K. I. and Cho, B. W. 1998. Determination of phylogenetic relationships of Korean native and Cheju nativ cttle to other breeds using PCR-RFLP of mtDNA D-loop region. Kor. J. Anim. Sci. 40:335-344
11 Mansson. B.. Geborek, P.. Saxne. T. and Bjornsson. S. 1990. Cytidine deaminase activity in synovial fluid of patients with rheumatoid arthritis: relation to lactoferrin. acidosis. and cartilage proteoglycan release. Ann Rheum Dis. 49. 594-597   DOI
12 Suzuki. T.. Yamauchi. K., Kawase, K., Tomita, M., Kiyosawa, I. and Dkonogi, S. 1989. Collaborative bacteriostatic activity of bovine lactoferrin with lysozyme against Escherichia coli 0111. Agric. Biol. Chem. 53:1705-1707   DOI
13 Tomita. M.. Takase, M.. Bellamy. W. and Shimamura. S. 1994. A review: the active peptide of lactoferrin. Acta Paediatr. Jpn. 36:585-591   DOI
14 Van der Kraan M. I. A. Groenink. J.. Nazmi. K.. Veerman. E. C.. Bolscher. J. G. and Nieuw Amerongen. A. V. 2004. Lactoferrampin: a novel antimicrobial peptide in the NI-domain of bovine lactoferrin. Peptides. 25: 177-183   DOI   ScienceOn
15 Ellison. R. T. 3rd and Giehl. T. J. 1991. Killing of gram-negative bacteria by lactoferrin and lysozyme. J. Clin. Invest. 88: 1080-1091   DOI
16 Bellamy. W. Takase. M.. Wakabayashi. H. Kawase. K. and Tomita. M. 1992. Antibacterial spectrum of lactoferricin B. a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. J. Appl. Bacteriol. 73:472-479   DOI
17 Tomita M.. Bellamy, W., Takase, M.. Yamauchi, K.. Wakabayashi. H. and Kawase, K. 1991. Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin. J. Dairy Sci. 74:4137-4142   DOI
18 Nemet. K. and Simonovits, I. 1985. The biological role of lactoferrin. Haematologia. 18:3-12
19 Rose. T. M.. Plowman, G. D.. Teplow, D. B.. Dreyer, W. J.. Hellstrom. K. E. and Brown. J. P. 1986. Primary structure of the human melanoma-associated antigen p97(melanotransferrin) deduced from the mRNA sequence. Proc. Natl. Acad. Sci. U.S.A.. 83:1261-1265
20 Broxmcyer. H. E.. DeSousa. M.. Smithyman, A.. Ralph. P. Hamilton. J., Kurland. J. I. and Bognacki. J. 1980. Specificity and modulation of the action of lactoferrin. a negative feedback regulator of myelopoiesis. Blood. 55:324-33
21 Yang. H. J., Son. D. H.. Ha. W. K. and Lee. S. W. 2005. Biochemical properties of lactoferrins from Korean native cow and bovine colostrum. Korean. J. Food Sci. Ani. Resour. 25:98-102
22 Woods. G. L. and Washington. J. A. 1995. Antibacterial susceptibility tests: Dilution and diffusion methods. : In Manual of clinical microbiology. P. R. Murray. E. J. Baron. M. A. Pfaller. F. C. Tenover. and R. H. Yolken (Ed.). 6th ed.. ASM Press. Washington D. C.. p1327-1341