Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Antimicrobial Activities of Lactoferrin and its Hydrolysate Obtained from the Colostrum of Hanwoo and Holstein Cattle

한우와 젖소 초유로부터 분리한 Lactoferrin과 가수분해물의 항균활성

  • Published : 2006.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study is to demonstrate antimicrobial activities of the lactoferrin and its peptic hydrolysates obtained from the colostrums of Hanwoo(Korean native cattle) and Holstein cattle. In the measurement of antimicrobial activity to E. coli O111 and other microorganisms, bovine lactoferrin showed a higher antimicrobial activity than that of Hanwoo cow's lactoferrin . The minimal inhibitory concentration (MIC) of lactoferrin against E. coli O111 was exhibited 1.5mg/ml(Holstein) and 2.75mg/ml (Hanwoo). The same result was also observed between bovine lactoferrin hydrolysate (0.12mg/ml) and Hanwoo cow's lactoferrin hydrolysate (0.25mg/ml). In addition of lysozyme, antimicrobial activity of lactoferrin was increased.

본 연구는 우리나라 재래종인 한우로부터 초유를 얻어 Lf을 분리정제한 후 한우 Lf와 젖소 Lf의 항균활성을 확인하였다. E. coli O111및 기타 미생물에 대한 항균성은 젖소 Lf가 한우 Lf 보다 높았으며, 젖소 Lf 가수분해물도 한우 Lf 가수분해물보다 마찬가지로 높았다. MIC에서는 E. coli O111 경우 젖소 Lf가 1.5mg/ml, 한우 Lf은 2.75mg/ml이며 젖소 Lf 가수분해물은 0.12mg/ml, 한우 Lf 가수분해물은 0.25mg/ml로 항균성 실험과 동일하게 젖소 Lf 가수분해물의 항균활성이 더 높은 것으로 나타났다. 한편 Lf과 lysozyme의 첨가는 항균활성을 상승시키는 효과를 나타내었다.

Keywords

References

  1. Aisen, P. and Listowsky, I. 1980. Iron transport and storage proteins. Annu. Rev. Biochem. 49
  2. Arnold. R. R.. Cole. M. F. and McGhee. J. R. 1977. A bactericidal effect for human Iactoferrin. Science. 197:263-265 https://doi.org/10.1126/science.327545
  3. Bellamy. W. Takase. M.. Wakabayashi. H. Kawase. K. and Tomita. M. 1992. Antibacterial spectrum of lactoferricin B. a potent bactericidal peptide derived from the N-terminal region of bovine lactoferrin. J. Appl. Bacteriol. 73:472-479 https://doi.org/10.1111/j.1365-2672.1992.tb05007.x
  4. Bellamy. W.. Wakabayashi. H., Takase, M.. Kawase. K.. Shimamura, S. and Tomita. M. 1993. Killing of Candida albicans by lactoferricin B. a potent antimicrobial peptide derived from the N-terminal region of bovine lactoferrin Med. Microbiol. Immunol. 182:97-105
  5. Bellamy. W.. Yamauchi. K.. Wakabayashi. H., Takase, M.. Takakura, N.. Shimamura, S. and M. Tomita. 1994. Antifungal properties of lactoferricin B. a peptide derived from the N-terminal region of bovine lactoferrcin. Lett. Appl. Microbiol. 18: 230-233 https://doi.org/10.1111/j.1472-765X.1994.tb00854.x
  6. Brock. J. H. 1985. Part I. I: Metal proteins with non-redox roles: In Metalloproteins. P. Harrison (Ed.). Macmillan Press. London. U.K., p183-262
  7. Broxmcyer. H. E.. DeSousa. M.. Smithyman, A.. Ralph. P. Hamilton. J., Kurland. J. I. and Bognacki. J. 1980. Specificity and modulation of the action of lactoferrin. a negative feedback regulator of myelopoiesis. Blood. 55:324-33
  8. Bullen. J. J. and Armstrong. J. A. 1979. The role of lactoferrin in the bactericidal function of polymorphonuclear leucocytes. Immunology. 36: 781-791
  9. Chen. J. P. and Wang. C. H. 1991. Microfiltration affinity purification of lactoferrin and immunoglobulin G from cheese whey. J. Food Sci. 56:701-706 https://doi.org/10.1111/j.1365-2621.1991.tb05360.x
  10. Dionysius. D. A.. Herse. J. B. and Grieve. P. A. 1991. Extraction of lactoperoxidase and lactoferrin from whey using batch ion exchange techniques. Aust, J. Dairy Technol. 46(Nov): 72-76
  11. Ellison. R. T. 3rd and Giehl. T. J. 1991. Killing of gram-negative bacteria by lactoferrin and lysozyme. J. Clin. Invest. 88: 1080-1091 https://doi.org/10.1172/JCI115407
  12. Gurr. M. I. 1981. Review of the progress of dairy science: human and artificial milks for infant feeding. J. Dairy Res. 48:519-554 https://doi.org/10.1017/S0022029900022020
  13. Lee. S. S.. Ko. S. B.. Oh, W. Y., Yang, Y. B.. Kim. K. I. and Cho, B. W. 1998. Determination of phylogenetic relationships of Korean native and Cheju nativ cttle to other breeds using PCR-RFLP of mtDNA D-loop region. Kor. J. Anim. Sci. 40:335-344
  14. Mansson. B.. Geborek, P.. Saxne. T. and Bjornsson. S. 1990. Cytidine deaminase activity in synovial fluid of patients with rheumatoid arthritis: relation to lactoferrin. acidosis. and cartilage proteoglycan release. Ann Rheum Dis. 49. 594-597 https://doi.org/10.1136/ard.49.8.594
  15. Nemet. K. and Simonovits, I. 1985. The biological role of lactoferrin. Haematologia. 18:3-12
  16. Rose. T. M.. Plowman, G. D.. Teplow, D. B.. Dreyer, W. J.. Hellstrom. K. E. and Brown. J. P. 1986. Primary structure of the human melanoma-associated antigen p97(melanotransferrin) deduced from the mRNA sequence. Proc. Natl. Acad. Sci. U.S.A.. 83:1261-1265
  17. Suzuki. T.. Yamauchi. K., Kawase, K., Tomita, M., Kiyosawa, I. and Dkonogi, S. 1989. Collaborative bacteriostatic activity of bovine lactoferrin with lysozyme against Escherichia coli 0111. Agric. Biol. Chem. 53:1705-1707 https://doi.org/10.1271/bbb1961.53.1705
  18. Tomita M.. Bellamy, W., Takase, M.. Yamauchi, K.. Wakabayashi. H. and Kawase, K. 1991. Potent antibacterial peptides generated by pepsin digestion of bovine lactoferrin. J. Dairy Sci. 74:4137-4142 https://doi.org/10.3168/jds.S0022-0302(91)78608-6
  19. Tomita. M.. Takase, M.. Bellamy. W. and Shimamura. S. 1994. A review: the active peptide of lactoferrin. Acta Paediatr. Jpn. 36:585-591 https://doi.org/10.1111/j.1442-200X.1994.tb03250.x
  20. Van der Kraan M. I. A. Groenink. J.. Nazmi. K.. Veerman. E. C.. Bolscher. J. G. and Nieuw Amerongen. A. V. 2004. Lactoferrampin: a novel antimicrobial peptide in the NI-domain of bovine lactoferrin. Peptides. 25: 177-183 https://doi.org/10.1016/j.peptides.2003.12.006
  21. Woods. G. L. and Washington. J. A. 1995. Antibacterial susceptibility tests: Dilution and diffusion methods. : In Manual of clinical microbiology. P. R. Murray. E. J. Baron. M. A. Pfaller. F. C. Tenover. and R. H. Yolken (Ed.). 6th ed.. ASM Press. Washington D. C.. p1327-1341
  22. Yang. H. J., Son. D. H.. Ha. W. K. and Lee. S. W. 2005. Biochemical properties of lactoferrins from Korean native cow and bovine colostrum. Korean. J. Food Sci. Ani. Resour. 25:98-102

Cited by

  1. Immunological Activity of Bovine Colostral Whey Protein Containing TGF-β from Imsil Province vol.32, pp.3, 2012, https://doi.org/10.5851/kosfa.2012.32.3.339