• 제목/요약/키워드: colostrum

검색결과 182건 처리시간 0.026초

한우 초유로부터 Lactoferrin의 분리.정제 (Isolation and Purification of Lactoferrin from Korean Native Cow's Colostrum)

  • 양희진;하월규;양동훈;박기문;이수원
    • 한국축산식품학회지
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    • 제20권2호
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    • pp.125-132
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    • 2000
  • Lactoferrin was isolated from the colostrum of Korean native cow by using several purification steps such as batch extraction, ion exchange chromatography, gel filtration chromatography and affinity chromatography. Other whey protein components that having similar molecular weight and affinity to lactoferrin were gradually removed from crude Korean native cow's lactoferrin during the purification steps. The amount of lactoferrin collected from a liter of Korean native cow's colostrum was 65mg and the recovery rate was 29.4%. The molecular weight of the purified Korean native cow's lactoferrin was estimated approximately 81,000dalton.

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발효초유사료 급여가 자돈의 성장에 미치는 영향 (Effects of Feeding Fermented Colostrum Feed on the Growth to Piglets)

  • 나석한;최성현;랜친핸드;배형철;남명수
    • 한국축산식품학회지
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    • 제28권3호
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    • pp.355-362
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    • 2008
  • 젖소 초유에서 427개의 유산균 집락을 분리하였고 이중 산 생성 및 당 이용성이 우수한 1번, 71번 집락을 16S rRNA gene sequence 834 bp의 분석결과 1번 집락은 99% S. macedonicus, 71번 집락은 16S rRNA gene sequence 736bp의 분석결과 99% S. thermophilus 균으로 밝혀졌다. 분리한 균은 각각 S. macedonicus mCNB-11와 S. thermophilus CNB-11로 명명하였다. S. macedonicus CNB-11와 S. thermophilus CNB-11의 발효특성을 L. fermentum과 비교하면 당 함량, pH, 적정산도, 유산균수 조사에서 L. fermentum CNB-11과 S. thermophilus CNB-11이 우수한 결과를 얻었다. 발효초유사료 0.5% 급여구가 대조구에 비해 증체율이 유의성 있게 16.73%증가하였다. 1일 증체량은 대조구에 비해 84.56 g이 높았다. 따라서 발효초유사료 급여에 따른 사양성적은 매우 우수한 것으로 확인되었다. 혈액성분 중 혈당, 콜레스테롤, 알부민, 글로블린의 양은 대조구와 발효초유사료 0.5% 급여구에서 차이는 나타나지 않았다. 자돈의 설사는 시험기간 4주간 설사증상을 보이는 개체는 대조구가 16.6%인데 비해 발효초유사료 0.5% 급여구에서는 전혀 나타나지 않았다.

국내산 초유의 가공방법에 따른 품질특성 연구 (Research on the Quality Characteristics of Domestic Colostrum according to the Processing Methods Employed)

  • 정석근;함준상;김동훈;안종남;채현석;유영모;장애라;권일경;이승규
    • 한국축산식품학회지
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    • 제29권4호
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    • pp.457-465
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    • 2009
  • 국내산 젖소 초유의 활용성을 높이기 위해 초유가공 방법별 특성을 분석한 결과를 요약하면 다음과 같다. 혼합초유의 유지방, 유단백질, 유당, 무지고형분은 각각 4.34, 6.99, 3.37, 11.10%이었다. 초유의 건조 전 총세균수와 대장균군수 수준은 각각 $3.0{\times}10^6$, $1.4{\times}10^3$ CFU/mL이었으나, 분무열풍 건조 후에는 각각 $8.8{\times}10^3$ CFU/mL과 음성으로 나타났으며, 동결 건조 시에는 각각 $3.7{\times}10^5$$4.0{\times}10^2$ CFU/mL으로 감소하는 것으로 나타났다. 초유의 품질을 향상시키기 위해 젖산균을 접종한 결과 자연발효처리 효과는 나타나지 않았으며, 저온 장시간 살균(LTLT) 후 젖산균을 접종한 처리구에서는 대장균군이 사멸하여 초유의 품질을 향상시킬 수 있었다. 산처리 초유보다는 젖산균접종 처리 초유가 대장균군의 억제효과는 높았으나, pH가 낮아 단백질 침전에 의한 유청 분리현상이 나타났다. 초유의 가공방법에 따른 IgG 함량의 영향을 평가하기 위해 LTLT 처리, 젖산 발효스타터, 분무건조, 동결건조를 실시한 결과 처리 전 초유 중 IgG 함량은 53.98 mg/mL이었으며, 처리 후에는 각각 33.28, 34.82, 21.98, 36.89 mg/mL로 감소하였다.

경기지역의 착유회수에 따른 초유의 이화학적 특성 (Physicochemical Properties of Colostrum by Milking Time of Gyeonggi Province)

  • 정석근;함준상;김동훈;안종남;채현석;유영모;장애라;권일경;이승규
    • 한국축산식품학회지
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    • 제29권4호
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    • pp.445-456
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    • 2009
  • 국내산 젖소 초유의 산차별, 착유 횟수별 성분특성을 구명하고 농가에서 이용 가능한 초유의 적정이용방법을 모색하여 잉여 초유의 저장성 향상 및 자원의 활용성을 높이고자 초유의 특성을 분석한 결과를 요약하면 다음과 같다. 초유의 유성분에 영향을 미치는 요인은 산차, 착유횟수, pH, 적정산도, 비중 등으로 확인되었다. 산차별 유성분의 변화를 보면 유지방은 산차에 따른 변화는 없었으며, 유단백질과 무지고형분은 산차가 높을수록 증가하였다. 분만 후 첫 회 착유한 초유의 유성분 중 유지방, 유단백질, 유당, 총고형분, 무지고형분은 각각 $6.16{\pm}2.39$, $14.78{\pm}4.30$, $2.57{\pm}0.77$, $24.28{\pm}4.36$, $18.12{\pm}4.08%$이었으며, 초유의 품질은 1-4회 착유분이 초유의 특성을 가지고 있었다. 초유의 착유횟수에 따른 체세포수는 분만 후 첫 회에는 $6.39{\pm}0.46$ log10 cell/mL 수준으로 매우 높게 나타나, 초유 납유시 체세포수를 고려한 품질관리에 관심을 가져야 할 것으로 나타났다. 분만 후 첫 회 착유한 초유의 aspartic acid, threonine, serine, glutamic acid, valine, leucine, lysine, 및 proline 등 주요 아미노산 함량은 각각 0.89, 0.71, 0.86, 1.75, 0.64, 0.95, 0.83, 및 0.95%이었고 착유횟수가 증가할수록 감소 하였다. 첫 회 착유한 초유의 myristic acid, palmitic acid, stearic acid, 및 oleic acid같은 주요 지방산 함량은 각각 총지방산의 14.3, 47.7, 7.41, 및 25.9%이었고, myristic acid, palmitic acid는 착유 횟수가 늘어남에 따라 증가하였으나 stearic acid와 oleic acid는 감소하였다. 첫 회 착유한 초유의 Ca, P, K, Na, Mg, Fe, Zn, 및 Cu함량은 각각 2,168, 1,959, 914, 761, 287, 1.7, 14.3, and 1.0 ppm이었고, 108시간 후 착유시에는 각각 1,389, 1,323, 838, 427, 131, 1.0, 4.7, 및 1.3 ppm으로 감소하였으며, Mn은 검출되지 않았다. 초유의 총 미생물수가 $3.0{\times}10^6$, 대장균군이 $1.4{\times}10^3$, 효 모곰팡이가 $1.6{\times}10^2$ CFU/mL이었다.

초유에 함유되어 있는 면역조절물질인 MIEF가 B 세포의 분화에 미치는 영향 (Induction of B Lymphocyte Differentiation by a Colostral Immunomodulatory Protein MIEF)

  • 이종호;이종길;한성순
    • 약학회지
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    • 제38권3호
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    • pp.351-357
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    • 1994
  • The levels of maternal immunity enhancing factor(MIEF), which is an immunomodulatory protein identified from bovine colostrum, were determined by indirect competitive enzyme-linked immunosorbent assay(ELISA) for the colostrum and normal milk collected during the first two weeks of lactation. The mean concentration of MIEF in the colostrum of the first day of lactation was $109\;{\mu}g/ml$, and fell from the third day of lactation to $3{\sim}4\;{\mu}g/ml$. The molecular weight of the purified MIEF determined by reducing SDS-PAGE and TSK G2000SW column chromatography was 22,000 and 24,000 daltons, respectively, showing that MIEF is a monomeric peptide in its native form. To examine the capacity of MIEF to induce differentiation of B Lymphocytes, human tonsillar Iymphocytes were cultured in the presence of different concentrations of MIEF, and then antibody secreting cells were enumerated by enzyme-linked immunospot(ELISPOT) assay. When added to cultures of human tonsillar Lymphocytes, MIEF induced differentiation of resting B Iymphocyte to antibody secreting plasma cells as efficiently as LPS.

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초유 유청분획의 EL-4 세포 증식 효과 (Effect of Colostral Whey Fraction on the Proliferation of EL-4 Cell)

  • 하월규;원도희;양희진;황경아;이수원
    • 한국축산식품학회지
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    • 제25권2호
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    • pp.244-249
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    • 2005
  • To investigate the effect of Holstein colostrum peptide fraction on proliferation of immune cell, polypeptide fractions were separated from acid whey into 3 fractions depending on molecular weight by ultrafiltration: Fraction I, which contains the polypeptide larger than 10,000 Da, Fraction n, which contains the polypeptide ranging from 1,000 Da to 10,000 Da and Fraction III, which contains the polypeptide smaller than 1,000 Da. EL-4 cell (murine T lymphoma cell) was used to evaluate immune enhancing effect of each fraction from Holstein colostrum. Fraction n showed the highest proliferative effect of the colostrum whey fractions on EL-4 cell at 1mg/mL compared with whole whey and other fractions and this proliferative activity was shown in dose dependent manner. Fraction n showed the highest proliferative effect on PMA (Phorbol 12-myristate 13-acetate) stimulated EL-4 cell. Heated Fraction n showed similar effect to native one on proliferation of both EL-4 cell and PMA stimulated EL-4 cell.

Comparative analysis of dietary exosome-derived microRNAs from human, bovine and caprine colostrum and mature milk

  • Yun, Bohyun;Kim, Younghoon;Park, Dong June;Oh, Sangnam
    • Journal of Animal Science and Technology
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    • 제63권3호
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    • pp.593-602
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    • 2021
  • Mammalian milk including microRNAs (miRNAs) as a novel class of noncoding RNAs, that can be transferred to infants and it plays on a critical role in biological functions such as immune regulation and development. However, the origin and functional importance of milk-derived miRNAs are still undetermined. This study applied RNA sequencing to explore the featured profiles of miRNA expression in colostrum and mature milk-originated exosomes from human, bovine, and caprine milk. These dietary exosome-derived miRNAs are highly conserved in human, bovine and caprine milk. Interestingly, abundant miRNAs expressed in human milk are similarly conserved across species. In addition, we confirmed that immune-related miRNAs (miR-30a-5p, miR-22-3p, and miR-26a) are commonly observed in the colostrum and mature milk of cows and caprines as well as humans. Our results provide new insights and resources for investigating the functionality of immune-associated miRNAs and evaluating physiological and biological condition in human, bovine and caprine milk as biomarkers.

한우 송아지의 초유섭취에 의한 수동면역 획득 상태 (A quantitative study of the transfer of colostral immunoglobulins to the newborn Korean native calf)

  • 김두;한홍율
    • 대한수의학회지
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    • 제29권2호
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    • pp.75-81
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    • 1989
  • The levels of the serum and colostral total protein and immunoglobulins of the Korean native cows immediately after parturition and colostrum-conferred passive immune status of the Korean native calves were studied, and the results obtained were summerized as follows: The mean total protein, total immunoglobulin, IgG, IgM, and IgA concentrations in sera of 10 Korean native cows immediately after parturition were $6.8{\pm}0.4g/dl$, $24.39{\pm}3.41mg/ml$, $19.49{\pm}2.70mg/ml$, $4.56{\pm}1.14mh/ml$, and $0.35{\pm}0.08mg/ml$ respectively. And the mean total protein, total immunoglobulin, IgG, IgM, and IgA concentrations in colostrum were $15.0{\pm}1.1g/dl$, $116.13{\pm}23.07mg/ml$, $101.51{\pm}22.59mg/ml$, $9.46{\pm}1.99mg/ml$, and $5.17{\pm}1.59mg/ml$ respectively. The mean concentrations of total protein and immunoglobulins in colostrum were 2.1 times to 14.8 times higher than those in serum. The mean concentrations of total protein, total immunoglobuln, IgG, IgM, and IgA in sera from 211 Korean native calves at 2 days old were $6.7{\pm}0.9g/dl$, $35.74{\pm}9.56mg/ml$, $29.06{\pm}8.07mg/ml$, $4.91{\pm}2.99mg/ml$, and $1,9{\pm}0.99mg/ml$ respectively. Although the calves had been suckled the dam's colostrum, each of calves was considerably various in levels of serum total protein and immunoglobulins and 16.1 percent of the calves were in a state of hypoglobulinemia.

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Characteristics of milk fever and mastitis following different milking method and preventive calcium injection performed during the colostrum period in dairy cattle

  • Jeon, Ryounghoon
    • 한국동물생명공학회지
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    • 제35권3호
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    • pp.273-278
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    • 2020
  • The milk fever and mastitis that occur during the colostrum period in dairy cattle cause great damage in the dairy industry by causing a rapid decline in the cattle's health and decreasing milk production. In order to prevent this, gradual milking (GM), calcium injection with gradual milking (CG), and calcium injection with complete milking (CC) methods are used. However, differences in effectiveness between these methods have not been established. Therefore, this study was conducted to determine the effects of each preventive method on milk fever and mastitis. The results of this study showed that while there was a significant negative correlation of GM with milk fever and mastitis (r = -0.657, p < 0.05), there was no correlation of CG and CC with milk fever and mastitis. The incidence of milk fever was significantly lower in CG and CC than in GM (p < 0.05) but no differences in the incidence of mastitis were observed between the three groups. No significant differences in the rate of recovery from milk fever and mastitis were observed between the three groups. The recurrence rate of milk fever was significantly higher in CG and CC than in GM (p < 0.05), though no differences in the recurrence rate of mastitis were observed. This study showed an inverse relationship between the incidence rates of milk fever and mastitis when calcium injection was not used during the colostrum period. When calcium injections were used, the incidence of milk fever was reduced without affecting the incidence of mastitis regardless of the milking method, but the recurrence rate of milk fever was increased. The results of this study would be useful for controlling blood calcium concentrations and establishing efficient milking strategy in order to effectively prevent milk fever and mastitis in dairy cattle during the colostrum period.

젖소 초유로부터 TGF-$\beta$1의 정제 (Purification of TGF-$\beta$1 from Bovine Colostrum)

  • 남명수;배형철;김평현;김완섭;고준수
    • 한국축산식품학회지
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    • 제22권4호
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    • pp.343-347
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    • 2002
  • TGF-$\beta$l은 여러 가지 생리활성 기능을 가지고 있기에 기능성식품 및 의약품 소재로 이용될 수 있다. 본 연구에서는 bovine colostrum milk로부터 TGF-$\beta$l을 분리 정제하기 위해 Cel-filtration chromatography, AF-heparin column chromatography 및 AF-heparin column rechromatography를 수행하여 TGF-$\beta$l을 정제하였다. 정제된 TGF-$\beta$1은 비환원조건하에서 전기영동을 수행하여 표준 TGF-$\beta$l과 같은 위치에 단일 band가 나타남으로 TGF-$\beta$l임을 확인하였다. 또한 환원 조건하에서 Western blot을 수행한 결과 TGF-$\beta$l 단일클론 항체와 결합하는 monomer 형태의 밴드를 확인하였다. 정제 TGF-$\beta$1의 회수율은 21%였다.