Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effects of Feeding Fermented Colostrum Feed on the Growth to Piglets

발효초유사료 급여가 자돈의 성장에 미치는 영향

  • Na, Seuk-Han (Chung Mi Bio Inc.) ;
  • Choi, Seong-Hyun (Chung Mi Bio Inc.) ;
  • Renchinthand, Gereltuya (Division of Animal Science & Resources, College of Agriculture & Life Science, Chungnam National University) ;
  • Bae, Hyoung-Churl (Division of Animal Science & Resources, College of Agriculture & Life Science, Chungnam National University) ;
  • Nam, Myoung-Soo (Division of Animal Science & Resources, College of Agriculture & Life Science, Chungnam National University)
  • 나석한 (㈜청미바이오) ;
  • 최성현 (㈜청미바이오) ;
  • 랜친핸드 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 배형철 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 남명수 (충남대학교 농업생명과학대학 동물자원과학부)
  • Published : 2008.08.30
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Abstract

This study was carried out to assess the fermentation properties of lactic acid bacteria (LAB) isolated from bovine colostrum and effects of feeding fermented colostrum feed on the growth to piglet. A total of 427 colonies were isolated from bovine colostrum on the BCP plate count agar. These LAB isolated were subcultured in 10% reconstituted skim milk, and seven strain thereof were selected for their highest acid productions, and two strain thereof were finally selected for their excellent sugar utilization. These strains were identified as Streptococcus thermophilus and Streptococcus macedonicus based on l6S rDNA sequencing data, named S. thermophilus CNB-11 and S. macedonicus CNB-11 respectively. For fermentation profiles, sugar utilization, acid production and viable cell counts were excellent in S. thermophilus CNB-11 as compared with S. macedoniclts CNB-11 after 48 hour. The effect of feeding fermented colostrum feed 0.5% using S. thermophilus CNB-11 was investigated for growth rate, analysis of blood and incidence of diarrhea. 24 heads of piglets were divided into two groups: the experimental and the control of 12 animals each. The average growth rate in the pigs fed fermented colostrum feed was higher 16.73% compared with control diet (p<0.05). There were no differences in the concentrations of blood glucose, cholesterol, albumin and globulin in pigs fed fermented colostrum feed as compared with control piglets. Incidence of diarrhea was no in pigs fed fermented colostrum feed as compared with control piglets.

젖소 초유에서 427개의 유산균 집락을 분리하였고 이중 산 생성 및 당 이용성이 우수한 1번, 71번 집락을 16S rRNA gene sequence 834 bp의 분석결과 1번 집락은 99% S. macedonicus, 71번 집락은 16S rRNA gene sequence 736bp의 분석결과 99% S. thermophilus 균으로 밝혀졌다. 분리한 균은 각각 S. macedonicus mCNB-11와 S. thermophilus CNB-11로 명명하였다. S. macedonicus CNB-11와 S. thermophilus CNB-11의 발효특성을 L. fermentum과 비교하면 당 함량, pH, 적정산도, 유산균수 조사에서 L. fermentum CNB-11과 S. thermophilus CNB-11이 우수한 결과를 얻었다. 발효초유사료 0.5% 급여구가 대조구에 비해 증체율이 유의성 있게 16.73%증가하였다. 1일 증체량은 대조구에 비해 84.56 g이 높았다. 따라서 발효초유사료 급여에 따른 사양성적은 매우 우수한 것으로 확인되었다. 혈액성분 중 혈당, 콜레스테롤, 알부민, 글로블린의 양은 대조구와 발효초유사료 0.5% 급여구에서 차이는 나타나지 않았다. 자돈의 설사는 시험기간 4주간 설사증상을 보이는 개체는 대조구가 16.6%인데 비해 발효초유사료 0.5% 급여구에서는 전혀 나타나지 않았다.

Keywords

References

  1. AOAC (1980) Official Methods of Analysis of the Association Official Analytical Chemists. Washington, DC
  2. Aparna, H. S. and Salimath, P. V. (1999) Acidic glycoproteins of buffalo colostrum and their influence on the growth of Bifidobacterium bifidus. Nutr. Res. 19, 295-303 https://doi.org/10.1016/S0271-5317(98)00192-4
  3. Bae, H. C. and Nam, M. S. (2005) Fermentation properties of the mixed yogurt prepared with bovine milk and soybean milk. Korean J. Food Sci. Anim. Resour. 25, 483-493
  4. Bae, H. C., Renchinthand, G., Na, S. H., Cho, S. H., and Nam, M. S. (2007) Studies on situation and utilization of domestic colostrum. Korean J. Food Sci. Ani. Resour. 27, 517-521 https://doi.org/10.5851/kosfa.2007.27.4.517
  5. Bae, H. C., Min, J. Y., Kim, K. W., and Nam, M. S. (2008) Probiotic effects of fermented mixture of prepared with milk and soybean use to Lactobacillus salivarius sp. salivarius DF20 on piglets. Korea Patent 10-0803532
  6. Besser, T. E. and Gay, C. C. (1994) The importance of colostrum to the health of the neonatal calf. Vet. Clin. Nor. Amer. - Food Anim. Pract. 10, 107-117 https://doi.org/10.1016/S0749-0720(15)30591-0
  7. Chang, Y. H., Kim, J. K., Kim, H. J., Kim, W. Y., Kim, Y. B., and Park, Y. H. (2000) Probiotic effects of Lactobacillus reuteri BSA-131 on piglets. Korean J. Appl. Microbiol. Biotechnol. 28, 8-13
  8. Donovan, S. M. and Odle, J. (1994) Growth factors in milk as mediators of infant development. Ann. Rev. Nutr. 14, 147-167 https://doi.org/10.1146/annurev.nu.14.070194.001051
  9. Felsenstein, J. (1993) PHYLIP: Phylogenetic Inference Package, version 3.5. Seattle: University of Washington
  10. Foley, J. A. and Otterby. D. E. (1978) Availability, storage, treatment, composition, and feeding value of surplus colostrum. A review. J. Dairy Sci. 61, 1033-1060 https://doi.org/10.3168/jds.S0022-0302(78)83686-8
  11. Jeon, K. S. and Hwang, I. K. (2002) The hydrolysis of isoflavones by Bifidobacterium sp. Int-57 during soymilk fermentation. Korean Soybean Digest. 19, 42-47
  12. Johnson, J. L. (1994) Similarity analysis of rRNAs. In: Methods for general and molecular bacteriology. Gerhardt, P. R., Murray, G. E., Wood, W. A., and Krirg, N. R. (eds), American Society for Microbiology, Washington, DC, pp. 683-700
  13. Kato, I. K., Endo, K., and Yokokura, T. (1994) Effects of oral administration of Lactobacillus casei on antitumor responses induced by tumor resection in mice. Int. J. Immunopharmacol. 16, 29-34 https://doi.org/10.1016/0192-0561(94)90116-3
  14. Kim, S. K. and Hoe, K. C. (2000) Effects of preservative treatment, heating and freezing on compositional and microbiological changes of colostrum. J. Anim. Sci. Technol. 42, 659-668
  15. Kyriakis, S. C., Tsiloyiannis, V. K., Vlemmas, J., Sarris, K., Tsinas, A. C., Alexopoulos, C., and Jansegers, L. (1999) The effect of probiotics LPS 122 on the control of post-weaning diarrhoea syndrome of piglets. Res. Vet. Sci. 67, 223-228 https://doi.org/10.1053/rvsc.1999.0308
  16. Lane, D. J. (1991) 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. In : Stackebrandt, E. and Goodfellow, M. (eds), John Wiley and Sons, Chichester, pp. 115-175
  17. Larson, L. L., Owen, F. G., Albright, J. L., Appleman, R. D., Lamb, R. C., and Muller, L. D. (1977) Guidelines toward more uniformity in measuring and reporting calf experimental data. J. Dairy Sci. 60, 989-1003 https://doi.org/10.3168/jds.S0022-0302(77)83975-1
  18. Maeng, W. J., Kim, C. W., and Shin, H. T. (1989) Effect of feeding lactic acid bacteria concentrate (LBC, Streptococcus faecium cernelle 68) on the growth rate and prevention of scouring in piglet. Korean J. Anim. Sci. 31, 318-323
  19. Mistuoka, T. (1990) Bifidobacteria and their role in human health. J. Ind. Microbiol. 6, 263-268 https://doi.org/10.1007/BF01575871
  20. Muralidhara, K. S., Sheggery, G. G., and Elliker, D. C. (1977) Effect of feeding lactobacillus on the coliform and lactobacillus flora of intestinal tissue and feces from piglets. J. Food Prot. 40, 288-295 https://doi.org/10.4315/0362-028X-40.5.288
  21. Nagao, F., Nakayama, M., Muto, T., and Okumura, K. (2000) Effects of a fermented milk drink containing Lactobacillus casei strain Shirota on the immune system in healthy human subjects. Biosci. Biotechnol. Biochem. 64, 2706-2708 https://doi.org/10.1271/bbb.64.2706
  22. Perdigon, G., Galdeano, C. M., and Valdez, J. C. (2002) Interaction of lactic acid bacteria with the gut immune system. Eur. J. Clin. Nutr. 56(suppl.), 21-26 https://doi.org/10.1038/sj.ejcn.1601278
  23. Pollmann, D. S., Danielson, D. M., and Jr. Peo, E. R. (1980) Effect of Lactobacillus acidophilus on starter pigs fed a diet supplemented with lactose. J. Anim. Sci. 51, 638-644 https://doi.org/10.2527/jas1980.513638x
  24. Reiter. B. (1978) Review of the progress of dairy science: Antimicrobial systems in milk. J. Dairy Res. 45, 131-147 https://doi.org/10.1017/S0022029900016290
  25. Sandine, W. E., Muralidhara, K. S., Elliker, P. R., and England, D. E. (1972) Lactic acid bacteria in food and health: A review with special reference to enteropathogenic Escherichia coli as well as certain enteric disease and their treatment with antibiotics and lactobacilli. J. Milk Food Technol. 35, 691-702 https://doi.org/10.4315/0022-2747-35.12.691
  26. Shams, D. (1994) Growth factors in milk. Endocrine Regulations 28, 3-8
  27. Tacket, C. O., Binion, S. B., Bostwick, E., Losonsky, G., Roy, M. J., and Edelman, R. (1992) Efficacy of bovine immunoglobulin concentrate in preventing illness after Shigella flexneri challenge. Am. J. Trop. Med. Hyg. 47, 276-283 https://doi.org/10.4269/ajtmh.1992.47.276
  28. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) CLUSTALW: improving the sensitive of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acid Res. 22, 4673-4680 https://doi.org/10.1093/nar/22.22.4673

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