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Purification of TGF-$\beta$1 from Bovine Colostrum  

남명수 (충남대학교 동물자원학부)
배형철 (충남대학교 동물자원학부)
김평현 (강원대학교 미생물학과)
김완섭 (강원대학교 축산가공학과)
고준수 (강원대학교 축산가공학과)
Publication Information
Food Science of Animal Resources / v.22, no.4, 2002 , pp. 343-347 More about this Journal
Abstract
Bovine colostrum contains various bio-functional Proteins. Especially, transforming: growth factor-${\beta}$1 (TGF-${\beta}$1) has a function in concerns with immune response. The purpose of this study was to establish the purification Processing of transforming growth factor-${\beta}$1(TGF-${\beta}$1). The highest concentration of TGF-${\beta}$1 was measured within 48 h after parturition in bovine colostrum using ELISA kit. Purification of TGF-${\beta}$1 from whey protein was carried out by the gel filtration, AF-heparin chromatography and AF-heparin rechromatography. After final purification step, TGF-${\beta}$1 with a molecular weight of 25 kD was obtained, and confirmed by silver staining and western blotting. Finally, TGF-${\beta}$1 was identified native form of 25 kD and reducing form of 12.5 kD by reducing agent.
Keywords
transforming growth factor-${\beta}$1; bio-functional proteins; bovine colostrum;
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