• 대한기계학회논문집B
• /
• 제36권4호
• /
• pp.391-395
• /
• 2012

본 논문에서는 서로 다른 밀도의 유체 내 바이오 물질이 받는 중력과 부력 차를 이용한 연속적 세포 분리기를 제안하였다. 종래의 크기별 세포분리는 서로 다른 크기의 동일한 밀도를 가지는 세포를 분리하는데 한계가 있다. 반면, 본 논문에서 제안하는 세포 분리기는 미소유로 상하부에 밀도가 다른 다층 유체층 내에서 세포가 받는 중력과 부력 차이로 크기는 다르지만 동일한 밀도를 가지는 세포를 효율적으로 분리할 수 있다. 밀도가 다른 유체층(PBS, 밀도=1.0g/ml, Ficoll, 밀도=1.1g/ml) 내에서 전혈로부터 백혈구(직경=$6-10{\mu}m$, 밀도=1.06~1.1g/ml), 적혈구(직경=$4-6{\mu}m$, 밀도=1.09~1.2g/ml)를 밀도에 따라 분리한 효율이 각각 $90.9{\pm}9.1%$와 $86.4{\pm}1.99%$로 측정되었다 따라서, 본 세포 분리기는 크기 편차가 있는 동일 밀도의 세포를 크기에 둔감하고 밀도에만 민감한 분리가 가능하다.

• 대한전자공학회논문지SD
• /
• 제41권5호
• /
• pp.107-114
• /
• 2004

본 논문에서는 CDMA 및 TDMA 기술을 기반으로 하는 무선 원격계측 시스템용 모뎀에 적합한 최적 하드웨어 구조 및 VLSI 설계에 대해서 제시한다. 무선 원격계측 시스템은 계측이 필요한 다수의 지점에 소형 무선계측기(RT: Remote Terminal)를 설치하고, 설치된 무선계측기로부터 계측데이터를 무선으로 수집하기 위해 제안된 시스템이다. 무선 원격계측 시스템은 계측데이터를 전송하기 위한 다수의 RT와 RT로부터 데이터를 수집하는 1개의 CU(Central Unit)로 구성된다. 본 논문에서는 이러한 RT와 CU용 모뎀에 적합한 최적 하드웨어 구조를 제안하였고, 제안된 구조를 Verilog HDL로 설계 및 검증하였다. Verilog HDL로 설계된 모뎀은 Synopsys/sup TM/툴을 이용하여 게이트 수준으로 합성되었고, 합성결과 RT 및 CU용 모뎀은 0.6㎛ 공정에서 각각 약 27K 게이트와 222K 게이트의 하드웨어 복잡도를 가졌다. 제안된 모뎀은 Altera/sup TM/ FPGA로 구현 및 검증되었다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제3권2호
• /
• pp.157-164
• /
• 1999

To determine molecular mechanisms of Aquaporin-CD (AQP2) gene regulation, the promoter region of the AQP2 gene was examined by transiently transfecting a promoter-luciferase reporter fusion gene into mouse renal collecting duct cell lines such as mIMCD-3, mIMCD-K2, and M-1 cells, and NIH3T3 mouse embryo fibroblast cells. PCR-Southern analysis reveals that mIMCD-3 and mIMCD-K2 cells express AQP2, but M-1 and NIH3T3 cells do not, and that the treatment with cpt-cAMP $(400\;{\mu}M)$) or forskolin/isobutylmethylxanthine (IBMX) increased the AQP2 expression in IMCD cells. In both IMCD and NIH3T3 cells, the constructs containing the promoter of AQP2 gene showed promoter activities, indicating lack of tissue-specific element in the 1.4 kb 5'-flanking region of the mouse AQP2 gene. Luciferase activity in the IMCD cells transfected with the construct containing 5-flanking region showed responsiveness to cpt-cAMP, indicating that the 1.4 kb 5'-flanking region contains the element necessary for the regulatory mechanism by cAMP. The promoter-luciferase constructs which do not have a cAMP-responsible element (CRE) still showed the cAMP responsiveness in IMCD cells, but not in NIH3T3 cells. Increase in medium osmolarity did not affect AQP2 promoter activity in mIMCD-K2 cells. These results demonstrate that AQP2 gene transcription is increased with cAMP treatment through multiple motifs including CRE in the 5'-flanking region of the gene in vitro, and the regulatory mechanism may be important for in vivo regulation of AQP2 expression.

• Childhood Kidney Diseases
• /
• 제20권1호
• /
• pp.11-17
• /
• 2016

Appropriate control of diet and water intake is important for maintaining normal blood pressure, fluid and electrolyte homeostasis in the body. It is relatively understood that the amount of sodium and potassium intake directly affects blood pressure and regulates ion transporters; Na and K channel functions in the kidney. However, little is known about whether diet and water intake regulates Aquaporin (AQP) function. AQPs, a family of aquaporin proteins with different types being expressed in different tissues, are important for water absorption by the cell. Water reabsorption is a passive process driven by osmotic gradient and water permeability is critical for this process. In most of the nephron, however, water reabsorption is unregulated and coupled to solute reabsorption, such as AQP1 mediated water absorption in the proximal tubule. AQP2 is the only water channel founded so far that can be regulated by hormones in the kidney. AQP2 expressed in the apical membrane of the principal cells in the collecting tubule can be regulated by vasopressin (antidiuretic hormone) controlling the final volume of urine excretion. When vasopressin binds to its receptor on the collecting duct cells, it stimulates the translocation of AQP2 to the membrane, leading to increased water absorption via this AQP2 water channel. However, some studies also indicated that the AQP2 is also been regulated by vasopressin independent mechanism. This review is focused on the regulation of AQP2 by diet and the amount of water intake on salt and water homeostasis.

• 한국고분자학회:학술대회논문집
• /
• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
• /
• pp.186-186
• /
• 2006

We report a new architecture for high efficiency polymer solar cells introducing a new concept of 'optical spacer' with new material. By implementing a novel solution-based titanium oxide ($TiO_{x}$) layer between the active layer and the electron collecting Al electrode, we invented a way to increase ${\sim}50\;%$ in power conversion efficiency compared to conventional polymer solar cells. Now the new devices exhibit ${\sim}6\;%$ power conversion efficiency, which is the highest value reported to date for a polymer based photovoltaic cell. The $TiO_{x}$ layer increases the efficiency by modifying the spatial distribution of the light intensity inside the device, thereby creating more photogenerated charge carriers in the bulk heterojunction layer.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2010년도 하계학술대회 논문집
• /
• pp.258-258
• /
• 2010

In this work, back bias effects in the program of the silicon-oxide-nitride-oxide-silicon (SONOS) cell using two-step pulse sequence, are investigated. Two-step pulse sequence is composed of the forward biases for collecting the electrons at the substrate terminal and back bias for injecting the hot electrons into the nitride layer. With an aid of the back bias for electron injection, we obtain a program time as short as 600 ns and an ultra low-voltage operation with a substrate voltage of -3 V.

• Toxicological Research
• /
• 제13권4호
• /
• pp.401-408
• /
• 1997

Adult male ICR mice were exposed to methylmercuric chloride (CH$_3$HgCI) through drinking water for 80 days. The distribution of mercury in the kidney, liver, spleen and cerebellum of the mouse was examined according to a autometallographic silver-enhancement technique based on a physical development process which renders mercury deposit visible. Grains of mercury traces were located in the proximal convoluted tubules. Lesser staining of the grains was seen in the collecting tubules of medulla. The glomerular basement membrane was void. In the liver, mercury accumulations were present primarily in the hepatocytes around portal area containing interlobular bile duct, artery and portal vein. Also grains of mercury traces were accumulated in the white pulp of the spleen and Purkinje cell layer of the cerebellum.

• 대한수의학회지
• /
• 제1권1호
• /
• pp.72.2-72.2
• /
• 1961

Since April, 1957, 200 White Leghorn and 100 Newhampshire had been brought to the poultry farm of our college from Songwhan National Breeding Station. The looses due to avian tuberculosis were 67 chickens by June, 1959. Thirteen of them were examined histopathologically. Grayish white tuberculous nodules varying in size were recognized in the liver, spleen, bone marrow, and intestine of all affected birds. The heart rind testis were involved in each case, and pulmonary tuberculosis which, unlike in mammals, was kown to be rarely encountered in birds was observed in two cases. One case showed amyloid like degeneration in hepatic cell cords of the liver, glomerular tufts and collecting ducts of the kidney. Lesions in the spleen presented typical Sago spleen. Sections from the liver, spleen, intestine, and lungs were stained by Ziel-Neelsen stain. Acid Fast stained bacilli were found embeded in the cytoplasm of epithlioid cells and foreign-body giant cells.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
• /
• pp.234-234
• /
• 2013

The counter electrodes in dye-sensitized solar cells (DSSCs) play roles in not only collecting electrons from external circuit but also reducing I3- to I- in electrolytes. Generally, conventional counter electrodes for DSSCs are prepared from the high temperature treatment of the H2PtCl6 precursor solution at $400^{\circ}C$ However, the more simplified fabrication process of counter electrodes is required for the commercialization of DSSCs. In this work, we developed novel fabrication process of counter electrodes using nano-second pulsed laser. DSSCs employing counter electrodes prepared by laser process showed conversion efficiency of 6.75% with short-circuit current of 12.73 mA/cm2, open-circuit voltage of 0.74 V and fill factor of 0.72. Closer investigating of photovoltaic properties will be reported.

• Transactions on Electrical and Electronic Materials
• /
• 제17권1호
• /
• pp.21-24
• /
• 2016

A junction box used in a 3 kW photovoltaic power generation system plays a role in collecting and supplying the direct current voltage produced by photovoltaic modules to an inverter. It is also used for facilitating maintenance checks and protecting the module and inverter by keeping the voltage constant. As for the junction box, using it in a parallel connection creates a difference between the setup modules. In order to compensate, an inverse voltage diode is used. But the high-power created through the solar generator can be delivered to the inverter through the inverter regularly. Therefore, a component can break down due to excess heat. And consequently short circuits and electric leakage occurs. In this study, using a junction box that enabled the bypass of high electric power, it was possible to reduce heat generation by approximately 35℃ when compared to a standard junction box.