• 한국미생물·생명공학회지
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• 제50권2호
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• pp.235-239
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• 2022

Psychrophiles have evolved to produce cold-adapted enzymes to enable survival in low-temperature environments. In this study, the cold adaptation of 5-enolpyruvylshikimate-3-phosphate synthase (CpsEPSPS) from Colwellia psychrerythraea, a model psychrophile, was analyzed. The optimum temperature for the activity of CpsEPSPS was found to be 25℃, with 35% activity remaining at 5℃. However, the unfolding temperature of CpsEPSPS was 54℃. This phenomenon is frequently observed in cold-active enzymes. As is the cases for most cold-active enzymes, the K_m values of CpsEPSPS for its substrates were higher than those of Escherichia coli EPSPS. These results indicate that CpsEPSPS is cold-adapted, but not perfectly.

• 동의생리병리학회지
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• 제19권5호
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• pp.1191-1194
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• 2005

The aim of this study was to investigate the change of Heart Rate Variability(HRV) that mild cold stimulus on the forehead of healthy men induces. 34 healthy male subjects participated in the experiments. On the first test series, 15 subjects were applied to the mild cold stimulus by the devise for cold stimulation. In the second test series, 10 subjects With heat Syndrome and 5 subjects With cold Syndrome were applied to the mild cold Stimulus. Aa a additional test, 4 subjects with cold syndrome were applied to the warm stimulus in the last test series. We analyzed the HRV through measuring electrocardiogram.(ECG). The result of this study is comparatively clear. In the first test series, mild cold stimulus made parasympathetic nervous system be activated. In the second test series, mild cold stimulus made parasympathetic nervous system be activated both in subjects with heat syndrome and subjects with cold syndrome, and heat syndrome shows more active parasympathetic nervous system rather than cold syndrome subjects do. In the last test series, 2 subjects with cold syndrome respond the mild cold stimulus. That means warm stimulus of cold condition subjects made parasympathetic nervous system active in 2 of 4 subjects. We found out that mild cold stimulus on forehead makes parasympathetic nervous system be activated in healthy male, and it can be interpreted that mild cold stimulus make healthy human be relaxed. Also, subjects have different fondness of thermal stimulus according to their Cold or Heat condition Preferences. Last test series shows that we need to investigate effect of the warm stimulus heat syndrome subjects.

• 한국초전도ㆍ저온공학회논문지
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• 제14권3호
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• pp.60-64
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• 2012

In this paper, an investigation of a room temperature active magnetic regenerative refrigerator is carried out. Experimental apparatus includes two active magnetic regenerators containing 186 g of Gd spheres. Four E-type thermocouples are installed inside the Active magnetic regenerator(AMR) to observe the instantaneous temperature variation of AMR. Both warm and cold heat exchangers are designed for large temperature span. The cold heat exchanger, which separates the two AMRs, employs a copper tube with length of 80 mm and diameter of 6.35 mm. In order to minimize dead volume between the warm heat exchanger and AMRs, the warm heat exchangers are located close to the AMRs. The deionized water is used as a heat transfer fluid, and maximum 1.4 T magnetic field is supplied by Halbach array of permanent magnets. The AMR plate, which contains the warm and the cold heat exchangers and the AMRs, has reciprocating motion using a linear actuator and each AMR is alternatively magnetized and demagnetized by a Halbach array of permanent magnet. Since the gap of the Halbach array of permanent magnets is 25 mm and two warm heat exchangers have the motion through it, a compact printed circuit heat exchanger (PCHE) is used as a warm heat exchanger. A maximum no-load temperature span of 26.8 K and a maximum cooling power of 33 W are obtained from the fabricated Active Magnetic Regenerative Refrigerator (AMRR).

• Journal of Microbiology and Biotechnology
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• 제25권5호
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• pp.681-686
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• 2015

The purpose of this study was to find a cold-adapted and surfactant-stable alginate lyase as a candidate for biotechnological and industrial applications. The gene for a new alginate lyase, AlyL1, from Agarivorans sp. L11 was cloned and expressed in Escherichia coli. The recombinant AlyL1 was most active at 40℃ (1,370 U/mg). It was a cold-adapted alginate lyase, which showed 54.5% and 72.1% of maximum activity at 15℃ and 20℃, respectively. AlyL1 was an alkaliphilic enzyme and most active at pH 8.6. In addition, it showed high stability in the presence of various surfactants at a high concentration (from 0.1% to 1% (w/v)). AlyL1 was an endo-type alginate lyase that degraded both polyM and polyG blocks, yielding disaccharides and trisaccharides as the main products. This is the first report of the cloning and functional expression of a cold-adapted and surfactant-stable alginate lyase. AlyL1 might be an interesting candidate for biotechnological and industrial applications.

• 한국지반환경공학회 논문집
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• 제17권1호
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• pp.49-54
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• 2016

동토지반을 구성하는 지층 중 표층에 분포하는 활동층은 계절에 따라 동결과 융해를 반복하여 지표면의 동상을 야기한다. 동상 높이는 활동층의 두께에 큰 영향을 받으므로, 동토지반 상부 인프라시설의 안전한 설계 및 시공을 위하여 활동층의 두께 산정은 매우 중요한 부분을 차지한다. 본 연구에서는 경량화된 원위치 관입시험 방법인 동적 콘 관입기를 이용하여 동토지반에서 활동층이 분포하는 심도를 평가하고자 하였다. 동적 콘 관입시험을 적용하기 위한 대상현장으로서 알래스카에 위치한 솔로몬 지역의 동토지반이 선택되었으며, 해당 지역의 두 개소에서 지중온도계측 및 동적 콘 관입시험이 수행되었다. 적용실험 결과 동적 콘 관입시험으로부터 획득된 동적 콘 관입지수는 활동층 및 영구동토층에서 서로 상이한 값을 나타내는바 동적 관입특성에 따른 활동층과 영구동토층의 경계부 심도가 산정되었으며, 경계부 심도에서 아이스 렌즈층으로 판단되는 구간이 감지되었다. 해당 개소에서 획득된 지중온도분포도에서 영상 및 영하 온도의 경계 심도는 본 연구의 동적 콘 관입시험으로부터 획득된 활동층 분포 심도와 부합하는 결과를 보였다. 본 연구에서 적용된 동적 콘 관입기는 대형장비의 접근 및 적용에 한계가 있는 극한지 동토 지역의 활동층 심도평가를 위하여 활용될 수 있을 것이라 기대된다.

• 미생물학회지
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• 제46권1호
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• pp.73-79
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• 2010

극지연구소(KOPRI)는 국내외적으로 유일하게 남북극 지역에서 분리한 저온적응성 박테리아 균주를 대상으로 culture collection(약 6,300균주)을 구축하여 운영하고 있다. 보유 중인 프로테아제(protease) 생산 균주들(총 874균주) 중에서 활성이 높은 프로테아제를 생산하는 78개의 균주들을 1차 선발한 후, 1% skim milk가 포함된 0.1${\times}$ ZoBell 고체배지에 접종하고 다양한 온도($5-30^{\circ}C$)에서 배양하면서 세포외분비성 프로테아제의 활성을 비교하였다. 위의 신속하고 직접적인 균주 스크리닝 방법을 통해서, 최종적으로 저온활성 프로테아제를 생산하는 15개의 저온적응성 균주들을 선발하였다. 최종 선발된 균주들은 16S rRNA 유전자의 분석결과 Pseudoalteromonas (13균주)와 Flavobacterium (2균주) 속(genus)으로 분류되었고, $5-15^{\circ}C$ 저온에서도 활성을 나타내는 저온성 프로테아제를 생산하였다. 15개 균주들이 생산하는 각각의 프로테아제는 특이적 화합물에 의한 효소활성 억제 정도에 따라 5개의 그룹(serine protease, aspartic protease, cysteine protease, metalloprotease, 그리고 미분류 프로테아제)으로 분류되었다. 본 실험을 통해서 선발한 남북극 유래 박테리아 균주들은 새로운 저온활성 프로테아제를 발굴하기 위한 유용한 생물자원으로서의 가치를 가지고 있다.

• 산업공학
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• 제25권2호
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• pp.153-162
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• 2012

The reliability is defined as a probability that a system will operate properly for a specified period of time under the design operating conditions without failure and it has been considered as one of the major design parameters in the field of industries. Reliability-Redundancy Optimization Problem(RROP) involves selec tion of components with multiple choices and redundancy levels for maximizing system reliability with constraints such as cost, weight, etc. However, in practice both active and cold standby redundancies may be used within a particular system design. Therefore, a redundancy strategy(active, cold standby) for each subsystem in order to maximize system reliability is considered in this study. Due to the nature of RROP, i.e. NP-hard problem, A Parallel Particle Swarm Optimization(PPSO) algorithm is proposed to solve the mathematical programming model and it gives consistently better quality solutions than existing studies for benchmark problems.

• 한국미생물·생명공학회지
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• 제50권1호
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• pp.71-80
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• 2022

Lipases (triacylglycerol acylhydrolases [EC 3.1.1.3]) are water-soluble enzymes. They catalyze the hydrolysis of fats and oils. A cold-active lipase from marine Bacillus cereus HSS, isolated from the Mediterranean Sea, Alexandria, Egypt, was purified and characterized. The total purification depending on lipase activity was 438.9 fold purification recording 632 U/mg protein. The molecular weight of the purified lipase was estimated to be 65 kDa using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The optimum substrate concentration, enzyme concentration, pH, and temperature were 1.5 mM, 100 µl, pH 6 and 10℃, respectively. The lipase was tolerant to NaCl concentrations ranging from 1.5 to 4.5%. The lipase was affected by the tested metal ions, and its activity was inhibited by 16% in the presence of 0.05 M SDS. The application of the cold-active lipase for the removal of an oil stain from a white cotton cloth showed that it is a promising biological agent for the treatment of oily wastes and other related applications. To the best of our knowledge, this is the first report of the purification and characterization of a lipase from marine B. cereus HSS isolated from the Mediterranean Sea.

• 약학회지
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• 제32권4호
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• pp.251-257
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• 1988

A simple and sensitive HPLC method was developed for the simultaneous determination of ten kinds of active ingredients formulated in commercial cough-cold mixtures. A group of Pseudoephedrine HCl, dl-Methylephedrine HCl, Noscapine, Chlorophenylamine maleate, Dextromethorphan HBr and Phenylpropanolamine HCl were determined at 254nm using a Novapak $C_{18}$ column with mobile phase consisting of a mixture of methanol-acetonitrile-1, 4dioxane-tetrahydrofuran-water(12 : 20 : 20 : 5 : 43, pH4.7) containing 0.013M-dioctyl sodium sulfosuccinate. The another group of Acetoaminophen, Caffeine, Guaifenesin and Ethenzamide were also determined at 254nm using a Novapak $C_{18}$ column as the stationary phase, and a mixture of methanol-1% aqueous acetic acid (3 : 7). The results indicate that these methods are accurate and precise with relative standard deviation of not more than 1% (n=5) for the above active ingredients.

• Ocean and Polar Research
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• 제27권2호
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• pp.221-230
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• 2005

Recently there has been a rapid accumulation of knowledge of microbial life in cold and frozen ecosystems. This understanding has revealed the extensive diversity of psychrophilic prokaryotes. Cultivation-based and molecular-based surveys have been performed in Antarctic habitats ranging from glacial ice to continental shelf sediments. Results indicate that psychrophilic taxa permeate throughout the Bacteria while they represent a more mysterious element of diversity in the Archaea owing to a notable lack of cultured strains. In certain cold climate ecosystems the diversity of psychrophilic populations reach levels comparable to the richest temperate equivalents. Within these communities must exist tremendous genetic diversify that is potentially of fundamental and of practical value. So far this genetic pool has been hardly explored. Only recently have genomic data become available for various psychrophilic prokaryotes and more is required. This owes to the fact that psychrophilic microbes possess manifold mechanisms for cold adaptations, which not only Provide enhanced survival and Persistence but Probably also contributes to niche specialisation. These mechanisms, including cold-active and ice-active proteins, polyunsaturated lipids and exopolysaccharides also have a great interest to biotechnologists.