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http://dx.doi.org/10.4014/jmb.1409.09031

Cloning, Expression, and Characterization of a Cold-Adapted and Surfactant-Stable Alginate Lyase from Marine Bacterium Agarivorans sp. L11  

Li, Shangyong (Key Laboratory of Marine Drugs)
Yang, Xuemei (Chinese Ministry of Education)
Zhang, Lan (Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology)
Yu, Wengong (School of Medicine and Pharmacy, Ocean University of China)
Han, Feng (School of Medicine and Pharmacy, Ocean University of China)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.5, 2015 , pp. 681-686 More about this Journal
Abstract
The purpose of this study was to find a cold-adapted and surfactant-stable alginate lyase as a candidate for biotechnological and industrial applications. The gene for a new alginate lyase, AlyL1, from Agarivorans sp. L11 was cloned and expressed in Escherichia coli. The recombinant AlyL1 was most active at 40℃ (1,370 U/mg). It was a cold-adapted alginate lyase, which showed 54.5% and 72.1% of maximum activity at 15℃ and 20℃, respectively. AlyL1 was an alkaliphilic enzyme and most active at pH 8.6. In addition, it showed high stability in the presence of various surfactants at a high concentration (from 0.1% to 1% (w/v)). AlyL1 was an endo-type alginate lyase that degraded both polyM and polyG blocks, yielding disaccharides and trisaccharides as the main products. This is the first report of the cloning and functional expression of a cold-adapted and surfactant-stable alginate lyase. AlyL1 might be an interesting candidate for biotechnological and industrial applications.
Keywords
Alginate lyase; cold-adapted; surfactant-stable; alkaliphilic; Agarivorans;
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