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http://dx.doi.org/10.48022/mbl.2201.01008

Characterization of 5-Enolpyruvylshikimate-3-Phosphate Synthase from Colwellia psychrerythraea  

Kim, Hak Jun (Department of Chemistry, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.50, no.2, 2022 , pp. 235-239 More about this Journal
Abstract
Psychrophiles have evolved to produce cold-adapted enzymes to enable survival in low-temperature environments. In this study, the cold adaptation of 5-enolpyruvylshikimate-3-phosphate synthase (CpsEPSPS) from Colwellia psychrerythraea, a model psychrophile, was analyzed. The optimum temperature for the activity of CpsEPSPS was found to be 25℃, with 35% activity remaining at 5℃. However, the unfolding temperature of CpsEPSPS was 54℃. This phenomenon is frequently observed in cold-active enzymes. As is the cases for most cold-active enzymes, the Km values of CpsEPSPS for its substrates were higher than those of Escherichia coli EPSPS. These results indicate that CpsEPSPS is cold-adapted, but not perfectly.
Keywords
Psychrophile; 5-enolpyruvylshikimate-3-phosphate synthase; cold adaptation; cold-active enzymes;
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