• 소성∙가공
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• 제12권2호
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• pp.123-127
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• 2003

The cold rolling conditions for the ultra thin steel for tension mask are very important because the residual stress that affects the flatness of strip is generate during the cold rolling. The residual stress in the sheet causes etching curls when it suffers perforation process. The residual stress through the thickness. To estimate the residual stress and deformation due to etching curl. FEM analysis is performed. Numerical simulation employ a ANSY5 5.6 and an elastic-plastic constitutive equation. The simulation results indicate the distribution of residual stress in the rolled sheet can be controlled by selecting the rolling conditions properly.

• 대한한방체열의학회지
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• 제2권1호
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• pp.17-23
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• 2003

Purpose The cold hypersensitivity is a subjective symptom and it is very difficult to evaluate the severity. It is possible to detect cold hypersensitivity by measuring the skin temperature on DITI, but there is limitation only using DITI to find the objective grade of the symptom. To set a new objective standard for the diagnosis of cold hypersensitivity, we examined the relationship between the Visual Analogue Scale (VAS) score for the cold hypersensitivity and the change of skin temperature on hands by cold stress test Method 23 patients with symptom of cold hypersensitivity were participated as subjects who visited the women medical center of Kangnam Kyunghee Korean Hospital, Kyung Hee Univ. from May 1, 2002 to August 31, 2002. There were all carefully examined to rule out other disease such as obesity, skin diseases, spinal nerve lesions and external wounds. Thermographic observations for this study were made using DITI. We performed cold stress test three times to compare with the results from thermographic observations by DITI: first, after 15 minutes-resting, second, right after 1 minutes soak in $20^{\circ}C$ water, the third for last, 10 minutes after the soak. VAS score was chosen to determine the severity of cold hypersensitivity. Result 1 male and 22 female patients were participated ranging in age from 22.17 to 45.21. There was a significant negative correlation between the recovery rate of finger skin temperature after cold stress test and the VAS score. And there was a significant positive correlation between the difference of finger skin temperature and the back and palm of hands after cold stress test and the VAS score. Conclusion In cold hypersensitivity patients, the cold stress test combined with DITI could be a accurate method for the objective evaluation of cold hypersensitivity, especially good at deciding the severity by numeric values. Using a more strict criterion, as diagnosing of cold hypersensitivity, and longer follow-up may improve the validity of the results attained in clinical trials.

• 한국작물학회지
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• 제49권4호
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• pp.289-294
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• 2004

This study was carried out to investigate the antioxidative enzymes and isozymes between chilling-tolerant and -susceptible varieties at the booting stage under cold water stress $(13^{\circ}C)$ in japonica rice. Total SOD, CAT, POX, and GR activities on the basis of protein were found to be important factors to defend cold water stress. Especially, SOD and CAT activities showed distinctive differences between chilling-tolerant and -susceptible varieties. Chilling-tolerant varieties were higher than chilling-susceptible varieties for SOD and CAT activities. One of eight isozyme bands for SOD was a inducible isoform. Three isozymes for CAT and one isozyme for POX were closely correlated with defense to cold water stress. Total GR activities except Stejaree 45 on the basis fresh weight and POX were increased by cold water stress, but there was no difference between chilling-tolerant and -sus­ceptible varieties.

• Korean Journal of Acupuncture
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• 제21권4호
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• pp.101-115
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• 2004

Objective : The present studies investigated the effects of 120 Hz high frequency electroacupuncture (EA) on the stress-induced stomach dysfunction in relation to its effect on the level of stress hormones and gastric mucosal damages. The gastric mucosal injury was induced by cold-restraint stress and two acupoints corresponding to Zusanli and Sanyinjiao in man were used. Methods : Cold-restraint stress produced typical gastric lesions in all rats of the stressed groups, but the number of ulcers as well as the mean ulcer diameter were reduced by 120 Hz EA pre-treatment. Results : The degranulation value of gastric mast cell was significantly higher in cold-restrained rats than in control ones. However, with the significant reduction of degranulation values of gastric mast cells in EA pre-treated rats compared with cold-restrained rats. Cold-restarint stress induced an elevated mRNA expression of pro-inflammatory gene such as cyclooxygenases-2 and tumor necrosis factor $(TNF)-{\alpha}$, but these expression were down-regulated in EA pre-treated rats. Immunohistochemical analysis showed that while the $inhibitory-{\kappa}B{\alpha}$ and $TNF-{\alpha}$ immunorection in the surface epithelium of the stomach tended to increase, both reactions in the EA pre-treated rats showed similar pattern as observed in controls. Conclusion : These results suggest that 120 Hz EA may act as a therapeutical means for gastric mucosal damages through an activation of pituitary adrenal system. It could be concluded that 120 Hz high frequency electroaucupuncture affords a good protective potential against stress-induced gastrointestinal dysfunction.

• 한국환경농학회지
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• 제36권2호
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• pp.106-112
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• 2017

BACKGROUND: Kale (Brassica oleracea) biosynthesizes various phytochemicals including glucosinolates, flavonoids, and carotenoids. Phytochemicals of plants are influenced by light, temperature, carbon dioxide, and growing conditions. Specifically, carotenoids are affected by temperature, light, and oxygen. The aim of this study was to investigate the effect of cold stress (day/night: $25^{\circ}C/20^{\circ}C$, $20^{\circ}C/15^{\circ}C$, $15^{\circ}C/10^{\circ}C$) on carotenoids in kale leaves. METHODS AND RESULTS: Kale was grown in pots for up to 50 days after sowing (DAS) in a greenhouse. For cold acclimation experiments, kale grown in growth chambers for 3 days and was subjected to low temperature for 4 days. The conditions maintained in the growth chambers were as follows: photoperiod, 12/12 h (day/night); light, fluorescent; and relative humidity, 60%. Carotenoid (lutein, ${\alpha}-carotene$, zeaxanthin, ${\beta}-carotene$) contents were analyzed by high-performance liquid chromatography (HPLC). The total carotenoid content gradually increased during cold acclimation for 3 days. When kale was subjected to cold stress, the total carotenoid content was high at $25^{\circ}C/20^{\circ}C$ treatment, but low at $15^{\circ}C/10^{\circ}C$ treatment. The total carotenoid content of kale leaves continuously grown in greenhouse decreased from 50 to 57 DAS (1,418 and 1,160 mgkg-1 dry wt., respectively). The lutein, ${\alpha}-carotene$, and ${\beta}-carotene$ contents were very low and the zeaxanthin contents were very high at $15^{\circ}C/10^{\circ}C$ treatment. When kale was subjected to cold stress, the ratio of individual to the total carotenoid contents of kale leaves was 4553% for -carotene and 210% for zeaxanthin. CONCLUSION: The ${\beta}-carotene$ and zeaxanthin contents in kale leaves indicate their sensitiveness toward cold stress.

• 한국정밀공학회지
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• 제17권10호
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• pp.77-82
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• 2000

The design of the prestressed cold extrusion die with two stress rings has been performed in this study. The cold extrusion has been simulated by the rigid-plastic FEM. The stress analysis of die has been performed for both after shrink fitting and during extrusion by using the elastic FEM and the Lame's equation. According to the variation of interferences and diameter ratios, the maximum effective stress has been evaluated. As results, interferences and diameters were determined by the minimization of the maximum effective stress of die insert. The comparison of the maximum effective stress between the proposed design and the conventional design has been discussed. It was found that the maximum effective stress in the die insert is considerably affected by the stiffness of the first stress ring.

• 대한기계학회논문집A
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• 제30권5호
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• pp.528-532
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• 2006

In the aerospace industry, Cold expansion has been used the most important method that is retarded of crack initiation from fastener hole surface. Cold expansion method(CEM) is that a oversized tapered mandrel goes through the hole in order to develop a compressive residual stress as the passing of the mandrel around the hole. Therefore, because of characteristic of mandrel inserting, Residual Stress Distributions (RSD) are differently generated form Entry, Mid and Exit position of the plate. Also, it is respected that RSD are changed as distances between holes. In this paper, It is performed a FE analysis of RSD by CEM and it is respectively shown RSD in the Entry, Mid and Exit position. It is compared residual stress results form the cold expansion in these two cases: the concurrent CEM and the sequential CEM. From this research, it has been found that compressive residual stress of Entry position is lower than other positions. Also, the concurrent CE of adjacent holes leads to much higher compressive residual stress than the sequential CE. In addition, in the sequential CE case, a compressive RSD of 1 step's hole around is lower than compressive RSD of 2 step's hole around.

• 미생물학회지
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• 제38권1호
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• pp.45-49
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• 2002

Bradyrhizobium japonicum 은 콩과 식물의 뿌리에 감염하여 뿌리흑을 형성 질소를 고정하는 독특한 능력을 갖는 토양 세균이며 미생물 비료제로 사용되고 있다. 본 연구에서는 저온에서 전처리한 B . japonicum 균주를 여러 가지 환경스트레스 조건에 노출하였을 때 생균수의 변화를 확인하였다. 저온 전처리는 16시간 동안 $4^{\circ}C$의 조건을 유지했다. 다양한 스트레스(알콜, 과산화수소, 고온, 건조)에 노출하였을 때, 저온 전처리한 것이 그렇지 않는 것보다 10~1,000배 정도 높은 생균수를 유지하였다. 이러한 내성중진 현상에 전처리 동안 새로운 단백질 합성이 수반되는 것을 단백질 합성 저해제 인 chloramphenicol을 전처리 과정에 포함하여 확인하였다. 저온 스트레스 내성에 관여하는 유전자를 B. japonicum genome 으로부터 중폭하였고 염기서열 분석을 실시하였다. 실험에서 확인된 B . japonicum의 CSP (Cold shock protein) 단백질의 부분적 아미노산 서열은 이미 확인된 다른 균주의 Csp 단백질과 유사함을 확인하였다.

• 대한기계학회논문집A
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• 제26권8호
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• pp.1615-1622
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• 2002

The purpose of this study is comparing cold expansion method with interference fit. Cold expansion method and interference fit of fastener hole is using in the aerospace industry. These treatment lead to an improvement of fatigue life to the compressive residual stresses developed on the hole surface. But Research is nothing to about difference effect of between cold expansion method and interference fit. So, this paper, it is shown that Comparing cold expansion method with interference fit using the finite element method. It is further shown that residual stress distribution according to plate thickness and clamping force.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 추계학술대회논문집A
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• pp.419-424
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• 2001

The purpose of this study is comparing cold expansion method with interference fit. Cold expansion method and interference fit of fastener hole is using in the aerospace industry. These treatment lead to an improvement of fatigue life to the compressive residual stresses developed on the hole surface. But Research is nothing to about difference effect of between cold expansion method and interference fit. So In this paper, It is shown that Comparing cold expansion method with interference fit. and It is further shown that residual stress distribution according to plate thickness.